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P58682

- TLR8_MOUSE

UniProt

P58682 - TLR8_MOUSE

Protein

Toll-like receptor 8

Gene

Tlr8

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. Acts via MYD88 and TRAF6, leading to NF-kappa-B. activation, cytokine secretion and the inflammatory response By similarity.By similarity

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB
    2. double-stranded RNA binding Source: UniProtKB
    3. drug binding Source: Ensembl
    4. single-stranded RNA binding Source: UniProtKB

    GO - Biological processi

    1. cellular response to mechanical stimulus Source: Ensembl
    2. defense response to virus Source: UniProtKB
    3. I-kappaB kinase/NF-kappaB signaling Source: Ensembl
    4. inflammatory response Source: UniProtKB-KW
    5. innate immune response Source: UniProtKB-KW
    6. microglial cell activation involved in immune response Source: Ensembl
    7. MyD88-dependent toll-like receptor signaling pathway Source: InterPro
    8. pathogen-associated molecular pattern dependent induction by symbiont of host innate immune response Source: Ensembl
    9. positive regulation of innate immune response Source: UniProtKB
    10. positive regulation of interferon-alpha biosynthetic process Source: UniProtKB
    11. positive regulation of interferon-beta biosynthetic process Source: UniProtKB
    12. positive regulation of interferon-gamma biosynthetic process Source: UniProtKB
    13. positive regulation of interleukin-8 biosynthetic process Source: UniProtKB
    14. regulation of cytokine secretion Source: InterPro
    15. toll-like receptor 8 signaling pathway Source: InterPro

    Keywords - Molecular functioni

    Receptor

    Keywords - Biological processi

    Immunity, Inflammatory response, Innate immunity

    Enzyme and pathway databases

    ReactomeiREACT_198527. TRAF6 mediated IRF7 activation in TLR7/8 or 9 signaling.
    REACT_198976. Trafficking and processing of endosomal TLR.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Toll-like receptor 8
    Alternative name(s):
    CD_antigen: CD288
    Gene namesi
    Name:Tlr8
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:2176887. Tlr8.

    Subcellular locationi

    Membrane By similarity; Single-pass type I membrane protein By similarity

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 10321009Toll-like receptor 8PRO_0000034736Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi29 – 291N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi36 ↔ 49By similarity
    Glycosylationi88 – 881N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi111 – 1111N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi122 – 1221N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi160 – 1601N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi177 ↔ 181By similarity
    Glycosylationi182 – 1821N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi231 – 2311N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi242 – 2421N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi252 ↔ 265By similarity
    Disulfide bondi255 ↔ 262By similarity
    Glycosylationi268 – 2681N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi288 – 2881N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi304 – 3041N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi353 – 3531N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi357 – 3571N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi411 – 4111N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi470 ↔ 500By similarity
    Glycosylationi502 – 5021N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi513 – 5131N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi581 – 5811N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi671 – 6711N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi743 – 7431N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi754 – 7541N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi783 – 7831N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiP58682.
    PRIDEiP58682.

    PTM databases

    PhosphoSiteiP58682.

    Expressioni

    Gene expression databases

    ArrayExpressiP58682.
    BgeeiP58682.
    CleanExiMM_TLR8.
    GenevestigatoriP58682.

    Interactioni

    Subunit structurei

    Homodimer. Interacts with MYD88 via their respective TIR domains By similarity. Interacts with BTK By similarity. Interacts with UNC93B1.By similarity1 Publication

    Protein-protein interaction databases

    MINTiMINT-4996881.

    Structurei

    3D structure databases

    ProteinModelPortaliP58682.
    SMRiP58682. Positions 31-808.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini24 – 818795ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini840 – 1032193CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei819 – 83921HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati41 – 6121LRR 1Add
    BLAST
    Repeati62 – 8524LRR 2Add
    BLAST
    Repeati87 – 10923LRR 3Add
    BLAST
    Repeati120 – 14324LRR 4Add
    BLAST
    Repeati145 – 16521LRR 5Add
    BLAST
    Repeati166 – 19429LRR 6Add
    BLAST
    Repeati195 – 21824LRR 7Add
    BLAST
    Repeati220 – 23920LRR 8Add
    BLAST
    Repeati240 – 26728LRR 9Add
    BLAST
    Repeati281 – 30424LRR 10Add
    BLAST
    Repeati306 – 32924LRR 11Add
    BLAST
    Repeati331 – 36030LRR 12Add
    BLAST
    Repeati361 – 38424LRR 13Add
    BLAST
    Repeati388 – 41124LRR 14Add
    BLAST
    Repeati413 – 43624LRR 15Add
    BLAST
    Repeati471 – 49424LRR 16Add
    BLAST
    Repeati520 – 54324LRR 17Add
    BLAST
    Repeati545 – 57228LRR 18Add
    BLAST
    Repeati574 – 59825LRR 19Add
    BLAST
    Repeati600 – 62122LRR 20Add
    BLAST
    Repeati629 – 65224LRR 21Add
    BLAST
    Repeati654 – 67724LRR 22Add
    BLAST
    Repeati678 – 70124LRR 23Add
    BLAST
    Repeati702 – 72524LRR 24Add
    BLAST
    Repeati727 – 74923LRR 25Add
    BLAST
    Repeati752 – 77625LRR 26Add
    BLAST
    Domaini869 – 1016148TIRPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the Toll-like receptor family.Curated
    Contains 26 LRR (leucine-rich) repeats.Curated
    Contains 1 TIR domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG4886.
    GeneTreeiENSGT00750000117479.
    HOGENOMiHOG000230468.
    HOVERGENiHBG018601.
    InParanoidiA2AHI9.
    KOiK10170.
    OMAiPATWFEN.
    OrthoDBiEOG7VB2DM.
    TreeFamiTF351113.

    Family and domain databases

    Gene3Di3.40.50.10140. 1 hit.
    InterProiIPR000483. Cys-rich_flank_reg_C.
    IPR001611. Leu-rich_rpt.
    IPR003591. Leu-rich_rpt_typical-subtyp.
    IPR026906. LRR_5.
    IPR000157. TIR_dom.
    IPR027175. TLR8.
    [Graphical view]
    PANTHERiPTHR24365:SF227. PTHR24365:SF227. 1 hit.
    PfamiPF00560. LRR_1. 1 hit.
    PF13306. LRR_5. 1 hit.
    PF13504. LRR_7. 1 hit.
    PF13855. LRR_8. 3 hits.
    PF01582. TIR. 1 hit.
    [Graphical view]
    SMARTiSM00369. LRR_TYP. 2 hits.
    SM00082. LRRCT. 1 hit.
    [Graphical view]
    SUPFAMiSSF52200. SSF52200. 1 hit.
    PROSITEiPS51450. LRR. 22 hits.
    PS50104. TIR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P58682-1 [UniParc]FASTAAdd to Basket

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    MENMPPQSWI LTCFCLLSSG TSAIFHKANY SRSYPCDEIR HNSLVIAECN     50
    HRQLHEVPQT IGKYVTNIDL SDNAITHITK ESFQKLQNLT KIDLNHNAKQ 100
    QHPNENKNGM NITEGALLSL RNLTVLLLED NQLYTIPAGL PESLKELSLI 150
    QNNIFQVTKN NTFGLRNLER LYLGWNCYFK CNQTFKVEDG AFKNLIHLKV 200
    LSLSFNNLFY VPPKLPSSLR KLFLSNAKIM NITQEDFKGL ENLTLLDLSG 250
    NCPRCYNAPF PCTPCKENSS IHIHPLAFQS LTQLLYLNLS STSLRTIPST 300
    WFENLSNLKE LHLEFNYLVQ EIASGAFLTK LPSLQILDLS FNFQYKEYLQ 350
    FINISSNFSK LRSLKKLHLR GYVFRELKKK HFEHLQSLPN LATINLGINF 400
    IEKIDFKAFQ NFSKLDVIYL SGNRIASVLD GTDYSSWRNR LRKPLSTDDD 450
    EFDPHVNFYH STKPLIKPQC TAYGKALDLS LNNIFIIGKS QFEGFQDIAC 500
    LNLSFNANTQ VFNGTEFSSM PHIKYLDLTN NRLDFDDNNA FSDLHDLEVL 550
    DLSHNAHYFS IAGVTHRLGF IQNLINLRVL NLSHNGIYTL TEESELKSIS 600
    LKELVFSGNR LDRLWNANDG KYWSIFKSLQ NLIRLDLSYN NLQQIPNGAF 650
    LNLPQSLQEL LISGNKLRFF NWTLLQYFPH LHLLDLSRNE LYFLPNCLSK 700
    FAHSLETLLL SHNHFSHLPS GFLSEARNLV HLDLSFNTIK MINKSSLQTK 750
    MKTNLSILEL HGNYFDCTCD ISDFRSWLDE NLNITIPKLV NVICSNPGDQ 800
    KSKSIMSLDL TTCVSDTTAA VLFFLTFLTT SMVMLAALVH HLFYWDVWFI 850
    YHMCSAKLKG YRTSSTSQTF YDAYISYDTK DASVTDWVIN ELRYHLEESE 900
    DKSVLLCLEE RDWDPGLPII DNLMQSINQS KKTIFVLTKK YAKSWNFKTA 950
    FYLALQRLMD ENMDVIIFIL LEPVLQYSQY LRLRQRICKS SILQWPNNPK 1000
    AENLFWQSLK NVVLTENDSR YDDLYIDSIR QY 1032
    Length:1,032
    Mass (Da):119,358
    Last modified:July 27, 2011 - v2
    Checksum:iCA192552F08E33EA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti613 – 6131R → H in AAK62677. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY035890 mRNA. Translation: AAK62677.1.
    AL731735 Genomic DNA. Translation: CAM14949.1.
    BC132054 mRNA. Translation: AAI32055.1.
    CCDSiCCDS41209.1.
    RefSeqiNP_573475.2. NM_133212.2.
    XP_006528780.1. XM_006528717.1.
    UniGeneiMm.196676.

    Genome annotation databases

    EnsembliENSMUST00000049023; ENSMUSP00000036762; ENSMUSG00000040522.
    ENSMUST00000112170; ENSMUSP00000107793; ENSMUSG00000040522.
    GeneIDi170744.
    KEGGimmu:170744.
    UCSCiuc009uwy.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY035890 mRNA. Translation: AAK62677.1 .
    AL731735 Genomic DNA. Translation: CAM14949.1 .
    BC132054 mRNA. Translation: AAI32055.1 .
    CCDSi CCDS41209.1.
    RefSeqi NP_573475.2. NM_133212.2.
    XP_006528780.1. XM_006528717.1.
    UniGenei Mm.196676.

    3D structure databases

    ProteinModelPortali P58682.
    SMRi P58682. Positions 31-808.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4996881.

    PTM databases

    PhosphoSitei P58682.

    Proteomic databases

    PaxDbi P58682.
    PRIDEi P58682.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000049023 ; ENSMUSP00000036762 ; ENSMUSG00000040522 .
    ENSMUST00000112170 ; ENSMUSP00000107793 ; ENSMUSG00000040522 .
    GeneIDi 170744.
    KEGGi mmu:170744.
    UCSCi uc009uwy.1. mouse.

    Organism-specific databases

    CTDi 51311.
    MGIi MGI:2176887. Tlr8.

    Phylogenomic databases

    eggNOGi COG4886.
    GeneTreei ENSGT00750000117479.
    HOGENOMi HOG000230468.
    HOVERGENi HBG018601.
    InParanoidi A2AHI9.
    KOi K10170.
    OMAi PATWFEN.
    OrthoDBi EOG7VB2DM.
    TreeFami TF351113.

    Enzyme and pathway databases

    Reactomei REACT_198527. TRAF6 mediated IRF7 activation in TLR7/8 or 9 signaling.
    REACT_198976. Trafficking and processing of endosomal TLR.

    Miscellaneous databases

    NextBioi 370310.
    PROi P58682.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P58682.
    Bgeei P58682.
    CleanExi MM_TLR8.
    Genevestigatori P58682.

    Family and domain databases

    Gene3Di 3.40.50.10140. 1 hit.
    InterProi IPR000483. Cys-rich_flank_reg_C.
    IPR001611. Leu-rich_rpt.
    IPR003591. Leu-rich_rpt_typical-subtyp.
    IPR026906. LRR_5.
    IPR000157. TIR_dom.
    IPR027175. TLR8.
    [Graphical view ]
    PANTHERi PTHR24365:SF227. PTHR24365:SF227. 1 hit.
    Pfami PF00560. LRR_1. 1 hit.
    PF13306. LRR_5. 1 hit.
    PF13504. LRR_7. 1 hit.
    PF13855. LRR_8. 3 hits.
    PF01582. TIR. 1 hit.
    [Graphical view ]
    SMARTi SM00369. LRR_TYP. 2 hits.
    SM00082. LRRCT. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52200. SSF52200. 1 hit.
    PROSITEi PS51450. LRR. 22 hits.
    PS50104. TIR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of murine Toll-like receptor 8."
      Heil F.J., Lipford G.B., Wagner H., Bauer S.M.
      Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Spleen.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    4. "Unc93B1 biases Toll-like receptor responses to nucleic acid in dendritic cells toward DNA- but against RNA-sensing."
      Fukui R., Saitoh S., Matsumoto F., Kozuka-Hata H., Oyama M., Tabeta K., Beutler B., Miyake K.
      J. Exp. Med. 206:1339-1350(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH UNC93B1.

    Entry informationi

    Entry nameiTLR8_MOUSE
    AccessioniPrimary (citable) accession number: P58682
    Secondary accession number(s): A2AHI9, Q91XI7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 31, 2002
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 115 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3