Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Toll-like receptor 8

Gene

Tlr8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. Acts via MYD88 and TRAF6, leading to NF-kappa-B. activation, cytokine secretion and the inflammatory response (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Immunity, Inflammatory response, Innate immunity

Enzyme and pathway databases

ReactomeiR-MMU-1679131. Trafficking and processing of endosomal TLR.
R-MMU-168181. Toll Like Receptor 7/8 (TLR7/8) Cascade.
R-MMU-975110. TRAF6 mediated IRF7 activation in TLR7/8 or 9 signaling.
R-MMU-975138. TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation.
R-MMU-975155. MyD88 dependent cascade initiated on endosome.

Names & Taxonomyi

Protein namesi
Recommended name:
Toll-like receptor 8
Alternative name(s):
CD_antigen: CD288
Gene namesi
Name:Tlr8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:2176887. Tlr8.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 818ExtracellularSequence analysisAdd BLAST795
Transmembranei819 – 839HelicalSequence analysisAdd BLAST21
Topological domaini840 – 1032CytoplasmicSequence analysisAdd BLAST193

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3137280.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000003473624 – 1032Toll-like receptor 8Add BLAST1009

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi29N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi36 ↔ 49By similarity
Glycosylationi88N-linked (GlcNAc...)Sequence analysis1
Glycosylationi111N-linked (GlcNAc...)Sequence analysis1
Glycosylationi122N-linked (GlcNAc...)Sequence analysis1
Glycosylationi160N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi177 ↔ 181By similarity
Glycosylationi182N-linked (GlcNAc...)Sequence analysis1
Glycosylationi231N-linked (GlcNAc...)Sequence analysis1
Glycosylationi242N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi252 ↔ 265By similarity
Disulfide bondi255 ↔ 262By similarity
Glycosylationi268N-linked (GlcNAc...)Sequence analysis1
Glycosylationi288N-linked (GlcNAc...)Sequence analysis1
Glycosylationi304N-linked (GlcNAc...)Sequence analysis1
Glycosylationi353N-linked (GlcNAc...)Sequence analysis1
Glycosylationi357N-linked (GlcNAc...)Sequence analysis1
Glycosylationi411N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi470 ↔ 500By similarity
Glycosylationi502N-linked (GlcNAc...)Sequence analysis1
Glycosylationi513N-linked (GlcNAc...)Sequence analysis1
Glycosylationi581N-linked (GlcNAc...)Sequence analysis1
Glycosylationi671N-linked (GlcNAc...)Sequence analysis1
Glycosylationi743N-linked (GlcNAc...)Sequence analysis1
Glycosylationi754N-linked (GlcNAc...)Sequence analysis1
Glycosylationi783N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP58682.
PaxDbiP58682.
PeptideAtlasiP58682.
PRIDEiP58682.

PTM databases

iPTMnetiP58682.
PhosphoSitePlusiP58682.

Expressioni

Gene expression databases

BgeeiENSMUSG00000040522.
CleanExiMM_TLR8.
ExpressionAtlasiP58682. baseline and differential.
GenevisibleiP58682. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with MYD88 via their respective TIR domains (By similarity). Interacts with BTK (By similarity). Interacts with UNC93B1. Interacts with SMPDL3B (PubMed:26095358).By similarity2 Publications

Protein-protein interaction databases

MINTiMINT-4996881.
STRINGi10090.ENSMUSP00000036762.

Structurei

3D structure databases

ProteinModelPortaliP58682.
SMRiP58682.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati41 – 61LRR 1Add BLAST21
Repeati62 – 85LRR 2Add BLAST24
Repeati87 – 109LRR 3Add BLAST23
Repeati120 – 143LRR 4Add BLAST24
Repeati145 – 165LRR 5Add BLAST21
Repeati166 – 194LRR 6Add BLAST29
Repeati195 – 218LRR 7Add BLAST24
Repeati220 – 239LRR 8Add BLAST20
Repeati240 – 267LRR 9Add BLAST28
Repeati281 – 304LRR 10Add BLAST24
Repeati306 – 329LRR 11Add BLAST24
Repeati331 – 360LRR 12Add BLAST30
Repeati361 – 384LRR 13Add BLAST24
Repeati388 – 411LRR 14Add BLAST24
Repeati413 – 436LRR 15Add BLAST24
Repeati471 – 494LRR 16Add BLAST24
Repeati520 – 543LRR 17Add BLAST24
Repeati545 – 572LRR 18Add BLAST28
Repeati574 – 598LRR 19Add BLAST25
Repeati600 – 621LRR 20Add BLAST22
Repeati629 – 652LRR 21Add BLAST24
Repeati654 – 677LRR 22Add BLAST24
Repeati678 – 701LRR 23Add BLAST24
Repeati702 – 725LRR 24Add BLAST24
Repeati727 – 749LRR 25Add BLAST23
Repeati752 – 776LRR 26Add BLAST25
Domaini869 – 1016TIRPROSITE-ProRule annotationAdd BLAST148

Sequence similaritiesi

Belongs to the Toll-like receptor family.Curated
Contains 26 LRR (leucine-rich) repeats.Curated
Contains 1 TIR domain.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4641. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00760000119006.
HOGENOMiHOG000230468.
HOVERGENiHBG018601.
InParanoidiP58682.
KOiK10170.
OMAiCFFTFFV.
OrthoDBiEOG091G014D.
TreeFamiTF351113.

Family and domain databases

Gene3Di3.40.50.10140. 1 hit.
3.80.10.10. 5 hits.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR026906. LRR_5.
IPR000157. TIR_dom.
IPR027175. TLR8.
[Graphical view]
PANTHERiPTHR24365:SF296. PTHR24365:SF296. 4 hits.
PfamiPF13306. LRR_5. 1 hit.
PF13855. LRR_8. 4 hits.
PF01582. TIR. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 13 hits.
SM00255. TIR. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
SSF52200. SSF52200. 1 hit.
PROSITEiPS51450. LRR. 22 hits.
PS50104. TIR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P58682-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENMPPQSWI LTCFCLLSSG TSAIFHKANY SRSYPCDEIR HNSLVIAECN
60 70 80 90 100
HRQLHEVPQT IGKYVTNIDL SDNAITHITK ESFQKLQNLT KIDLNHNAKQ
110 120 130 140 150
QHPNENKNGM NITEGALLSL RNLTVLLLED NQLYTIPAGL PESLKELSLI
160 170 180 190 200
QNNIFQVTKN NTFGLRNLER LYLGWNCYFK CNQTFKVEDG AFKNLIHLKV
210 220 230 240 250
LSLSFNNLFY VPPKLPSSLR KLFLSNAKIM NITQEDFKGL ENLTLLDLSG
260 270 280 290 300
NCPRCYNAPF PCTPCKENSS IHIHPLAFQS LTQLLYLNLS STSLRTIPST
310 320 330 340 350
WFENLSNLKE LHLEFNYLVQ EIASGAFLTK LPSLQILDLS FNFQYKEYLQ
360 370 380 390 400
FINISSNFSK LRSLKKLHLR GYVFRELKKK HFEHLQSLPN LATINLGINF
410 420 430 440 450
IEKIDFKAFQ NFSKLDVIYL SGNRIASVLD GTDYSSWRNR LRKPLSTDDD
460 470 480 490 500
EFDPHVNFYH STKPLIKPQC TAYGKALDLS LNNIFIIGKS QFEGFQDIAC
510 520 530 540 550
LNLSFNANTQ VFNGTEFSSM PHIKYLDLTN NRLDFDDNNA FSDLHDLEVL
560 570 580 590 600
DLSHNAHYFS IAGVTHRLGF IQNLINLRVL NLSHNGIYTL TEESELKSIS
610 620 630 640 650
LKELVFSGNR LDRLWNANDG KYWSIFKSLQ NLIRLDLSYN NLQQIPNGAF
660 670 680 690 700
LNLPQSLQEL LISGNKLRFF NWTLLQYFPH LHLLDLSRNE LYFLPNCLSK
710 720 730 740 750
FAHSLETLLL SHNHFSHLPS GFLSEARNLV HLDLSFNTIK MINKSSLQTK
760 770 780 790 800
MKTNLSILEL HGNYFDCTCD ISDFRSWLDE NLNITIPKLV NVICSNPGDQ
810 820 830 840 850
KSKSIMSLDL TTCVSDTTAA VLFFLTFLTT SMVMLAALVH HLFYWDVWFI
860 870 880 890 900
YHMCSAKLKG YRTSSTSQTF YDAYISYDTK DASVTDWVIN ELRYHLEESE
910 920 930 940 950
DKSVLLCLEE RDWDPGLPII DNLMQSINQS KKTIFVLTKK YAKSWNFKTA
960 970 980 990 1000
FYLALQRLMD ENMDVIIFIL LEPVLQYSQY LRLRQRICKS SILQWPNNPK
1010 1020 1030
AENLFWQSLK NVVLTENDSR YDDLYIDSIR QY
Length:1,032
Mass (Da):119,358
Last modified:July 27, 2011 - v2
Checksum:iCA192552F08E33EA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti613R → H in AAK62677 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY035890 mRNA. Translation: AAK62677.1.
AL731735 Genomic DNA. Translation: CAM14949.1.
BC132054 mRNA. Translation: AAI32055.1.
CCDSiCCDS41209.1.
RefSeqiNP_001300689.1. NM_001313760.1.
NP_001300690.1. NM_001313761.1.
NP_573475.2. NM_133212.3.
UniGeneiMm.196676.

Genome annotation databases

EnsembliENSMUST00000049023; ENSMUSP00000036762; ENSMUSG00000040522.
ENSMUST00000112170; ENSMUSP00000107793; ENSMUSG00000040522.
GeneIDi170744.
KEGGimmu:170744.
UCSCiuc009uwy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY035890 mRNA. Translation: AAK62677.1.
AL731735 Genomic DNA. Translation: CAM14949.1.
BC132054 mRNA. Translation: AAI32055.1.
CCDSiCCDS41209.1.
RefSeqiNP_001300689.1. NM_001313760.1.
NP_001300690.1. NM_001313761.1.
NP_573475.2. NM_133212.3.
UniGeneiMm.196676.

3D structure databases

ProteinModelPortaliP58682.
SMRiP58682.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4996881.
STRINGi10090.ENSMUSP00000036762.

Chemistry databases

ChEMBLiCHEMBL3137280.

PTM databases

iPTMnetiP58682.
PhosphoSitePlusiP58682.

Proteomic databases

MaxQBiP58682.
PaxDbiP58682.
PeptideAtlasiP58682.
PRIDEiP58682.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000049023; ENSMUSP00000036762; ENSMUSG00000040522.
ENSMUST00000112170; ENSMUSP00000107793; ENSMUSG00000040522.
GeneIDi170744.
KEGGimmu:170744.
UCSCiuc009uwy.1. mouse.

Organism-specific databases

CTDi51311.
MGIiMGI:2176887. Tlr8.

Phylogenomic databases

eggNOGiKOG4641. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00760000119006.
HOGENOMiHOG000230468.
HOVERGENiHBG018601.
InParanoidiP58682.
KOiK10170.
OMAiCFFTFFV.
OrthoDBiEOG091G014D.
TreeFamiTF351113.

Enzyme and pathway databases

ReactomeiR-MMU-1679131. Trafficking and processing of endosomal TLR.
R-MMU-168181. Toll Like Receptor 7/8 (TLR7/8) Cascade.
R-MMU-975110. TRAF6 mediated IRF7 activation in TLR7/8 or 9 signaling.
R-MMU-975138. TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation.
R-MMU-975155. MyD88 dependent cascade initiated on endosome.

Miscellaneous databases

PROiP58682.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040522.
CleanExiMM_TLR8.
ExpressionAtlasiP58682. baseline and differential.
GenevisibleiP58682. MM.

Family and domain databases

Gene3Di3.40.50.10140. 1 hit.
3.80.10.10. 5 hits.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR026906. LRR_5.
IPR000157. TIR_dom.
IPR027175. TLR8.
[Graphical view]
PANTHERiPTHR24365:SF296. PTHR24365:SF296. 4 hits.
PfamiPF13306. LRR_5. 1 hit.
PF13855. LRR_8. 4 hits.
PF01582. TIR. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 13 hits.
SM00255. TIR. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
SSF52200. SSF52200. 1 hit.
PROSITEiPS51450. LRR. 22 hits.
PS50104. TIR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTLR8_MOUSE
AccessioniPrimary (citable) accession number: P58682
Secondary accession number(s): A2AHI9, Q91XI7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.