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P58682 (TLR8_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Toll-like receptor 8
Alternative name(s):
CD_antigen=CD288
Gene names
Name:Tlr8
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1032 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. Acts via MYD88 and TRAF6, leading to NF-kappa-B. activation, cytokine secretion and the inflammatory response By similarity.

Subunit structure

Homodimer. Interacts with MYD88 via their respective TIR domains By similarity. Interacts with BTK By similarity. Interacts with UNC93B1. Ref.4

Subcellular location

Membrane; Single-pass type I membrane protein By similarity.

Sequence similarities

Belongs to the Toll-like receptor family.

Contains 26 LRR (leucine-rich) repeats.

Contains 1 TIR domain.

Ontologies

Keywords
   Biological processImmunity
Inflammatory response
Innate immunity
   Cellular componentMembrane
   DomainLeucine-rich repeat
Repeat
Signal
Transmembrane
Transmembrane helix
   Molecular functionReceptor
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processI-kappaB kinase/NF-kappaB signaling

Inferred from electronic annotation. Source: Ensembl

cellular response to mechanical stimulus

Inferred from electronic annotation. Source: Ensembl

defense response to virus

Inferred from sequence or structural similarity. Source: UniProtKB

inflammatory response

Inferred from electronic annotation. Source: UniProtKB-KW

innate immune response

Inferred from electronic annotation. Source: UniProtKB-KW

microglial cell activation involved in immune response

Inferred from electronic annotation. Source: Ensembl

pathogen-associated molecular pattern dependent induction by symbiont of host innate immune response

Inferred from electronic annotation. Source: Ensembl

positive regulation of innate immune response

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of interferon-alpha biosynthetic process

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of interferon-beta biosynthetic process

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of interferon-gamma biosynthetic process

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of interleukin-8 biosynthetic process

Inferred from sequence or structural similarity. Source: UniProtKB

toll-like receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from sequence or structural similarity. Source: UniProtKB

double-stranded RNA binding

Inferred from sequence or structural similarity. Source: UniProtKB

drug binding

Inferred from electronic annotation. Source: Ensembl

single-stranded RNA binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 10321009Toll-like receptor 8
PRO_0000034736

Regions

Topological domain24 – 818795Extracellular Potential
Transmembrane819 – 83921Helical; Potential
Topological domain840 – 1032193Cytoplasmic Potential
Repeat41 – 6121LRR 1
Repeat62 – 8524LRR 2
Repeat87 – 10923LRR 3
Repeat120 – 14324LRR 4
Repeat145 – 16521LRR 5
Repeat166 – 19429LRR 6
Repeat195 – 21824LRR 7
Repeat220 – 23920LRR 8
Repeat240 – 26728LRR 9
Repeat281 – 30424LRR 10
Repeat306 – 32924LRR 11
Repeat331 – 36030LRR 12
Repeat361 – 38424LRR 13
Repeat388 – 41124LRR 14
Repeat413 – 43624LRR 15
Repeat471 – 49424LRR 16
Repeat520 – 54324LRR 17
Repeat545 – 57228LRR 18
Repeat574 – 59825LRR 19
Repeat600 – 62122LRR 20
Repeat629 – 65224LRR 21
Repeat654 – 67724LRR 22
Repeat678 – 70124LRR 23
Repeat702 – 72524LRR 24
Repeat727 – 74923LRR 25
Repeat752 – 77625LRR 26
Domain869 – 1016148TIR

Amino acid modifications

Glycosylation291N-linked (GlcNAc...) Potential
Glycosylation881N-linked (GlcNAc...) Potential
Glycosylation1111N-linked (GlcNAc...) Potential
Glycosylation1221N-linked (GlcNAc...) Potential
Glycosylation1601N-linked (GlcNAc...) Potential
Glycosylation1821N-linked (GlcNAc...) Potential
Glycosylation2311N-linked (GlcNAc...) Potential
Glycosylation2421N-linked (GlcNAc...) Potential
Glycosylation2681N-linked (GlcNAc...) Potential
Glycosylation2881N-linked (GlcNAc...) Potential
Glycosylation3041N-linked (GlcNAc...) Potential
Glycosylation3531N-linked (GlcNAc...) Potential
Glycosylation3571N-linked (GlcNAc...) Potential
Glycosylation4111N-linked (GlcNAc...) Potential
Glycosylation5021N-linked (GlcNAc...) Potential
Glycosylation5131N-linked (GlcNAc...) Potential
Glycosylation5811N-linked (GlcNAc...) Potential
Glycosylation6711N-linked (GlcNAc...) Potential
Glycosylation7431N-linked (GlcNAc...) Potential
Glycosylation7541N-linked (GlcNAc...) Potential
Glycosylation7831N-linked (GlcNAc...) Potential
Disulfide bond36 ↔ 49 By similarity
Disulfide bond177 ↔ 181 By similarity
Disulfide bond252 ↔ 265 By similarity
Disulfide bond255 ↔ 262 By similarity
Disulfide bond470 ↔ 500 By similarity

Experimental info

Sequence conflict6131R → H in AAK62677. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P58682 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: CA192552F08E33EA

FASTA1,032119,358
        10         20         30         40         50         60 
MENMPPQSWI LTCFCLLSSG TSAIFHKANY SRSYPCDEIR HNSLVIAECN HRQLHEVPQT 

        70         80         90        100        110        120 
IGKYVTNIDL SDNAITHITK ESFQKLQNLT KIDLNHNAKQ QHPNENKNGM NITEGALLSL 

       130        140        150        160        170        180 
RNLTVLLLED NQLYTIPAGL PESLKELSLI QNNIFQVTKN NTFGLRNLER LYLGWNCYFK 

       190        200        210        220        230        240 
CNQTFKVEDG AFKNLIHLKV LSLSFNNLFY VPPKLPSSLR KLFLSNAKIM NITQEDFKGL 

       250        260        270        280        290        300 
ENLTLLDLSG NCPRCYNAPF PCTPCKENSS IHIHPLAFQS LTQLLYLNLS STSLRTIPST 

       310        320        330        340        350        360 
WFENLSNLKE LHLEFNYLVQ EIASGAFLTK LPSLQILDLS FNFQYKEYLQ FINISSNFSK 

       370        380        390        400        410        420 
LRSLKKLHLR GYVFRELKKK HFEHLQSLPN LATINLGINF IEKIDFKAFQ NFSKLDVIYL 

       430        440        450        460        470        480 
SGNRIASVLD GTDYSSWRNR LRKPLSTDDD EFDPHVNFYH STKPLIKPQC TAYGKALDLS 

       490        500        510        520        530        540 
LNNIFIIGKS QFEGFQDIAC LNLSFNANTQ VFNGTEFSSM PHIKYLDLTN NRLDFDDNNA 

       550        560        570        580        590        600 
FSDLHDLEVL DLSHNAHYFS IAGVTHRLGF IQNLINLRVL NLSHNGIYTL TEESELKSIS 

       610        620        630        640        650        660 
LKELVFSGNR LDRLWNANDG KYWSIFKSLQ NLIRLDLSYN NLQQIPNGAF LNLPQSLQEL 

       670        680        690        700        710        720 
LISGNKLRFF NWTLLQYFPH LHLLDLSRNE LYFLPNCLSK FAHSLETLLL SHNHFSHLPS 

       730        740        750        760        770        780 
GFLSEARNLV HLDLSFNTIK MINKSSLQTK MKTNLSILEL HGNYFDCTCD ISDFRSWLDE 

       790        800        810        820        830        840 
NLNITIPKLV NVICSNPGDQ KSKSIMSLDL TTCVSDTTAA VLFFLTFLTT SMVMLAALVH 

       850        860        870        880        890        900 
HLFYWDVWFI YHMCSAKLKG YRTSSTSQTF YDAYISYDTK DASVTDWVIN ELRYHLEESE 

       910        920        930        940        950        960 
DKSVLLCLEE RDWDPGLPII DNLMQSINQS KKTIFVLTKK YAKSWNFKTA FYLALQRLMD 

       970        980        990       1000       1010       1020 
ENMDVIIFIL LEPVLQYSQY LRLRQRICKS SILQWPNNPK AENLFWQSLK NVVLTENDSR 

      1030 
YDDLYIDSIR QY 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of murine Toll-like receptor 8."
Heil F.J., Lipford G.B., Wagner H., Bauer S.M.
Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Spleen.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[4]"Unc93B1 biases Toll-like receptor responses to nucleic acid in dendritic cells toward DNA- but against RNA-sensing."
Fukui R., Saitoh S., Matsumoto F., Kozuka-Hata H., Oyama M., Tabeta K., Beutler B., Miyake K.
J. Exp. Med. 206:1339-1350(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH UNC93B1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY035890 mRNA. Translation: AAK62677.1.
AL731735 Genomic DNA. Translation: CAM14949.1.
BC132054 mRNA. Translation: AAI32055.1.
CCDSCCDS41209.1.
RefSeqNP_573475.2. NM_133212.2.
XP_006528780.1. XM_006528717.1.
UniGeneMm.196676.

3D structure databases

ProteinModelPortalP58682.
SMRP58682. Positions 31-808.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4996881.

PTM databases

PhosphoSiteP58682.

Proteomic databases

PaxDbP58682.
PRIDEP58682.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000049023; ENSMUSP00000036762; ENSMUSG00000040522.
ENSMUST00000112170; ENSMUSP00000107793; ENSMUSG00000040522.
GeneID170744.
KEGGmmu:170744.
UCSCuc009uwy.1. mouse.

Organism-specific databases

CTD51311.
MGIMGI:2176887. Tlr8.

Phylogenomic databases

eggNOGCOG4886.
GeneTreeENSGT00750000117479.
HOGENOMHOG000230468.
HOVERGENHBG018601.
InParanoidA2AHI9.
KOK10170.
OMAPATWFEN.
OrthoDBEOG7VB2DM.
TreeFamTF351113.

Gene expression databases

ArrayExpressP58682.
BgeeP58682.
CleanExMM_TLR8.
GenevestigatorP58682.

Family and domain databases

Gene3D3.40.50.10140. 1 hit.
InterProIPR000483. Cys-rich_flank_reg_C.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR026906. LRR_5.
IPR000157. TIR_dom.
IPR027175. TLR8.
[Graphical view]
PANTHERPTHR24365:SF227. PTHR24365:SF227. 1 hit.
PfamPF00560. LRR_1. 1 hit.
PF13306. LRR_5. 1 hit.
PF13504. LRR_7. 1 hit.
PF13855. LRR_8. 3 hits.
PF01582. TIR. 1 hit.
[Graphical view]
SMARTSM00369. LRR_TYP. 2 hits.
SM00082. LRRCT. 1 hit.
[Graphical view]
SUPFAMSSF52200. SSF52200. 1 hit.
PROSITEPS51450. LRR. 22 hits.
PS50104. TIR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio370310.
PROP58682.
SOURCESearch...

Entry information

Entry nameTLR8_MOUSE
AccessionPrimary (citable) accession number: P58682
Secondary accession number(s): A2AHI9, Q91XI7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot