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P58682

- TLR8_MOUSE

UniProt

P58682 - TLR8_MOUSE

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Protein

Toll-like receptor 8

Gene

Tlr8

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. Acts via MYD88 and TRAF6, leading to NF-kappa-B. activation, cytokine secretion and the inflammatory response (By similarity).By similarity

GO - Molecular functioni

  1. DNA binding Source: UniProtKB
  2. double-stranded RNA binding Source: UniProtKB
  3. drug binding Source: Ensembl
  4. single-stranded RNA binding Source: UniProtKB

GO - Biological processi

  1. cellular response to mechanical stimulus Source: Ensembl
  2. defense response to virus Source: UniProtKB
  3. I-kappaB kinase/NF-kappaB signaling Source: Ensembl
  4. inflammatory response Source: UniProtKB-KW
  5. innate immune response Source: UniProtKB-KW
  6. microglial cell activation involved in immune response Source: Ensembl
  7. MyD88-dependent toll-like receptor signaling pathway Source: InterPro
  8. pathogen-associated molecular pattern dependent induction by symbiont of host innate immune response Source: Ensembl
  9. positive regulation of innate immune response Source: UniProtKB
  10. positive regulation of interferon-alpha biosynthetic process Source: UniProtKB
  11. positive regulation of interferon-beta biosynthetic process Source: UniProtKB
  12. positive regulation of interferon-gamma biosynthetic process Source: UniProtKB
  13. positive regulation of interleukin-8 biosynthetic process Source: UniProtKB
  14. regulation of cytokine secretion Source: InterPro
  15. toll-like receptor 8 signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Immunity, Inflammatory response, Innate immunity

Enzyme and pathway databases

ReactomeiREACT_198527. TRAF6 mediated IRF7 activation in TLR7/8 or 9 signaling.
REACT_198976. Trafficking and processing of endosomal TLR.
REACT_229712. TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation.
REACT_242921. Toll Like Receptor 7/8 (TLR7/8) Cascade.
REACT_256727. MyD88 dependent cascade initiated on endosome.

Names & Taxonomyi

Protein namesi
Recommended name:
Toll-like receptor 8
Alternative name(s):
CD_antigen: CD288
Gene namesi
Name:Tlr8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:2176887. Tlr8.

Subcellular locationi

Membrane By similarity; Single-pass type I membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 818795ExtracellularSequence AnalysisAdd
BLAST
Transmembranei819 – 83921HelicalSequence AnalysisAdd
BLAST
Topological domaini840 – 1032193CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 10321009Toll-like receptor 8PRO_0000034736Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi29 – 291N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi36 ↔ 49By similarity
Glycosylationi88 – 881N-linked (GlcNAc...)Sequence Analysis
Glycosylationi111 – 1111N-linked (GlcNAc...)Sequence Analysis
Glycosylationi122 – 1221N-linked (GlcNAc...)Sequence Analysis
Glycosylationi160 – 1601N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi177 ↔ 181By similarity
Glycosylationi182 – 1821N-linked (GlcNAc...)Sequence Analysis
Glycosylationi231 – 2311N-linked (GlcNAc...)Sequence Analysis
Glycosylationi242 – 2421N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi252 ↔ 265By similarity
Disulfide bondi255 ↔ 262By similarity
Glycosylationi268 – 2681N-linked (GlcNAc...)Sequence Analysis
Glycosylationi288 – 2881N-linked (GlcNAc...)Sequence Analysis
Glycosylationi304 – 3041N-linked (GlcNAc...)Sequence Analysis
Glycosylationi353 – 3531N-linked (GlcNAc...)Sequence Analysis
Glycosylationi357 – 3571N-linked (GlcNAc...)Sequence Analysis
Glycosylationi411 – 4111N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi470 ↔ 500By similarity
Glycosylationi502 – 5021N-linked (GlcNAc...)Sequence Analysis
Glycosylationi513 – 5131N-linked (GlcNAc...)Sequence Analysis
Glycosylationi581 – 5811N-linked (GlcNAc...)Sequence Analysis
Glycosylationi671 – 6711N-linked (GlcNAc...)Sequence Analysis
Glycosylationi743 – 7431N-linked (GlcNAc...)Sequence Analysis
Glycosylationi754 – 7541N-linked (GlcNAc...)Sequence Analysis
Glycosylationi783 – 7831N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP58682.
PRIDEiP58682.

PTM databases

PhosphoSiteiP58682.

Expressioni

Gene expression databases

BgeeiP58682.
CleanExiMM_TLR8.
ExpressionAtlasiP58682. baseline and differential.
GenevestigatoriP58682.

Interactioni

Subunit structurei

Homodimer. Interacts with MYD88 via their respective TIR domains (By similarity). Interacts with BTK (By similarity). Interacts with UNC93B1.By similarity1 Publication

Protein-protein interaction databases

MINTiMINT-4996881.

Structurei

3D structure databases

ProteinModelPortaliP58682.
SMRiP58682. Positions 31-808.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati41 – 6121LRR 1Add
BLAST
Repeati62 – 8524LRR 2Add
BLAST
Repeati87 – 10923LRR 3Add
BLAST
Repeati120 – 14324LRR 4Add
BLAST
Repeati145 – 16521LRR 5Add
BLAST
Repeati166 – 19429LRR 6Add
BLAST
Repeati195 – 21824LRR 7Add
BLAST
Repeati220 – 23920LRR 8Add
BLAST
Repeati240 – 26728LRR 9Add
BLAST
Repeati281 – 30424LRR 10Add
BLAST
Repeati306 – 32924LRR 11Add
BLAST
Repeati331 – 36030LRR 12Add
BLAST
Repeati361 – 38424LRR 13Add
BLAST
Repeati388 – 41124LRR 14Add
BLAST
Repeati413 – 43624LRR 15Add
BLAST
Repeati471 – 49424LRR 16Add
BLAST
Repeati520 – 54324LRR 17Add
BLAST
Repeati545 – 57228LRR 18Add
BLAST
Repeati574 – 59825LRR 19Add
BLAST
Repeati600 – 62122LRR 20Add
BLAST
Repeati629 – 65224LRR 21Add
BLAST
Repeati654 – 67724LRR 22Add
BLAST
Repeati678 – 70124LRR 23Add
BLAST
Repeati702 – 72524LRR 24Add
BLAST
Repeati727 – 74923LRR 25Add
BLAST
Repeati752 – 77625LRR 26Add
BLAST
Domaini869 – 1016148TIRPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the Toll-like receptor family.Curated
Contains 26 LRR (leucine-rich) repeats.Curated
Contains 1 TIR domain.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG4886.
GeneTreeiENSGT00760000119006.
HOGENOMiHOG000230468.
HOVERGENiHBG018601.
InParanoidiP58682.
KOiK10170.
OMAiPATWFEN.
OrthoDBiEOG7VB2DM.
TreeFamiTF351113.

Family and domain databases

Gene3Di3.40.50.10140. 1 hit.
InterProiIPR000483. Cys-rich_flank_reg_C.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR026906. LRR_5.
IPR000157. TIR_dom.
IPR027175. TLR8.
[Graphical view]
PANTHERiPTHR24365:SF227. PTHR24365:SF227. 1 hit.
PfamiPF00560. LRR_1. 1 hit.
PF13306. LRR_5. 1 hit.
PF13504. LRR_7. 1 hit.
PF13855. LRR_8. 3 hits.
PF01582. TIR. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 2 hits.
SM00082. LRRCT. 1 hit.
[Graphical view]
SUPFAMiSSF52200. SSF52200. 1 hit.
PROSITEiPS51450. LRR. 22 hits.
PS50104. TIR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P58682-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MENMPPQSWI LTCFCLLSSG TSAIFHKANY SRSYPCDEIR HNSLVIAECN
60 70 80 90 100
HRQLHEVPQT IGKYVTNIDL SDNAITHITK ESFQKLQNLT KIDLNHNAKQ
110 120 130 140 150
QHPNENKNGM NITEGALLSL RNLTVLLLED NQLYTIPAGL PESLKELSLI
160 170 180 190 200
QNNIFQVTKN NTFGLRNLER LYLGWNCYFK CNQTFKVEDG AFKNLIHLKV
210 220 230 240 250
LSLSFNNLFY VPPKLPSSLR KLFLSNAKIM NITQEDFKGL ENLTLLDLSG
260 270 280 290 300
NCPRCYNAPF PCTPCKENSS IHIHPLAFQS LTQLLYLNLS STSLRTIPST
310 320 330 340 350
WFENLSNLKE LHLEFNYLVQ EIASGAFLTK LPSLQILDLS FNFQYKEYLQ
360 370 380 390 400
FINISSNFSK LRSLKKLHLR GYVFRELKKK HFEHLQSLPN LATINLGINF
410 420 430 440 450
IEKIDFKAFQ NFSKLDVIYL SGNRIASVLD GTDYSSWRNR LRKPLSTDDD
460 470 480 490 500
EFDPHVNFYH STKPLIKPQC TAYGKALDLS LNNIFIIGKS QFEGFQDIAC
510 520 530 540 550
LNLSFNANTQ VFNGTEFSSM PHIKYLDLTN NRLDFDDNNA FSDLHDLEVL
560 570 580 590 600
DLSHNAHYFS IAGVTHRLGF IQNLINLRVL NLSHNGIYTL TEESELKSIS
610 620 630 640 650
LKELVFSGNR LDRLWNANDG KYWSIFKSLQ NLIRLDLSYN NLQQIPNGAF
660 670 680 690 700
LNLPQSLQEL LISGNKLRFF NWTLLQYFPH LHLLDLSRNE LYFLPNCLSK
710 720 730 740 750
FAHSLETLLL SHNHFSHLPS GFLSEARNLV HLDLSFNTIK MINKSSLQTK
760 770 780 790 800
MKTNLSILEL HGNYFDCTCD ISDFRSWLDE NLNITIPKLV NVICSNPGDQ
810 820 830 840 850
KSKSIMSLDL TTCVSDTTAA VLFFLTFLTT SMVMLAALVH HLFYWDVWFI
860 870 880 890 900
YHMCSAKLKG YRTSSTSQTF YDAYISYDTK DASVTDWVIN ELRYHLEESE
910 920 930 940 950
DKSVLLCLEE RDWDPGLPII DNLMQSINQS KKTIFVLTKK YAKSWNFKTA
960 970 980 990 1000
FYLALQRLMD ENMDVIIFIL LEPVLQYSQY LRLRQRICKS SILQWPNNPK
1010 1020 1030
AENLFWQSLK NVVLTENDSR YDDLYIDSIR QY
Length:1,032
Mass (Da):119,358
Last modified:July 27, 2011 - v2
Checksum:iCA192552F08E33EA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti613 – 6131R → H in AAK62677. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY035890 mRNA. Translation: AAK62677.1.
AL731735 Genomic DNA. Translation: CAM14949.1.
BC132054 mRNA. Translation: AAI32055.1.
CCDSiCCDS41209.1.
RefSeqiNP_573475.2. NM_133212.2.
XP_006528780.1. XM_006528717.1.
UniGeneiMm.196676.

Genome annotation databases

EnsembliENSMUST00000049023; ENSMUSP00000036762; ENSMUSG00000040522.
ENSMUST00000112170; ENSMUSP00000107793; ENSMUSG00000040522.
GeneIDi170744.
KEGGimmu:170744.
UCSCiuc009uwy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY035890 mRNA. Translation: AAK62677.1 .
AL731735 Genomic DNA. Translation: CAM14949.1 .
BC132054 mRNA. Translation: AAI32055.1 .
CCDSi CCDS41209.1.
RefSeqi NP_573475.2. NM_133212.2.
XP_006528780.1. XM_006528717.1.
UniGenei Mm.196676.

3D structure databases

ProteinModelPortali P58682.
SMRi P58682. Positions 31-808.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-4996881.

Chemistry

ChEMBLi CHEMBL3137280.

PTM databases

PhosphoSitei P58682.

Proteomic databases

PaxDbi P58682.
PRIDEi P58682.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000049023 ; ENSMUSP00000036762 ; ENSMUSG00000040522 .
ENSMUST00000112170 ; ENSMUSP00000107793 ; ENSMUSG00000040522 .
GeneIDi 170744.
KEGGi mmu:170744.
UCSCi uc009uwy.1. mouse.

Organism-specific databases

CTDi 51311.
MGIi MGI:2176887. Tlr8.

Phylogenomic databases

eggNOGi COG4886.
GeneTreei ENSGT00760000119006.
HOGENOMi HOG000230468.
HOVERGENi HBG018601.
InParanoidi P58682.
KOi K10170.
OMAi PATWFEN.
OrthoDBi EOG7VB2DM.
TreeFami TF351113.

Enzyme and pathway databases

Reactomei REACT_198527. TRAF6 mediated IRF7 activation in TLR7/8 or 9 signaling.
REACT_198976. Trafficking and processing of endosomal TLR.
REACT_229712. TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation.
REACT_242921. Toll Like Receptor 7/8 (TLR7/8) Cascade.
REACT_256727. MyD88 dependent cascade initiated on endosome.

Miscellaneous databases

NextBioi 370310.
PROi P58682.
SOURCEi Search...

Gene expression databases

Bgeei P58682.
CleanExi MM_TLR8.
ExpressionAtlasi P58682. baseline and differential.
Genevestigatori P58682.

Family and domain databases

Gene3Di 3.40.50.10140. 1 hit.
InterProi IPR000483. Cys-rich_flank_reg_C.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR026906. LRR_5.
IPR000157. TIR_dom.
IPR027175. TLR8.
[Graphical view ]
PANTHERi PTHR24365:SF227. PTHR24365:SF227. 1 hit.
Pfami PF00560. LRR_1. 1 hit.
PF13306. LRR_5. 1 hit.
PF13504. LRR_7. 1 hit.
PF13855. LRR_8. 3 hits.
PF01582. TIR. 1 hit.
[Graphical view ]
SMARTi SM00369. LRR_TYP. 2 hits.
SM00082. LRRCT. 1 hit.
[Graphical view ]
SUPFAMi SSF52200. SSF52200. 1 hit.
PROSITEi PS51450. LRR. 22 hits.
PS50104. TIR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of murine Toll-like receptor 8."
    Heil F.J., Lipford G.B., Wagner H., Bauer S.M.
    Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Spleen.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. "Unc93B1 biases Toll-like receptor responses to nucleic acid in dendritic cells toward DNA- but against RNA-sensing."
    Fukui R., Saitoh S., Matsumoto F., Kozuka-Hata H., Oyama M., Tabeta K., Beutler B., Miyake K.
    J. Exp. Med. 206:1339-1350(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH UNC93B1.

Entry informationi

Entry nameiTLR8_MOUSE
AccessioniPrimary (citable) accession number: P58682
Secondary accession number(s): A2AHI9, Q91XI7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: July 27, 2011
Last modified: November 26, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3