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P58681 (TLR7_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Toll-like receptor 7
Gene names
Name:Tlr7
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length1050 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Ref.2

Subunit structure

Interacts with MYD88 via their respective TIR domains By similarity. Interacts with UNC93B1. Ref.3

Subcellular location

Endoplasmic reticulum membrane; Single-pass type I membrane protein. Endosome. Lysosome. Cytoplasmic vesicle › phagosome. Note: Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist. Ref.5

Sequence similarities

Belongs to the Toll-like receptor family.

Contains 28 LRR (leucine-rich) repeats.

Contains 1 TIR domain.

Ontologies

Keywords
   Biological processImmunity
Inflammatory response
Innate immunity
   Cellular componentCytoplasmic vesicle
Endoplasmic reticulum
Endosome
Lysosome
Membrane
   DomainLeucine-rich repeat
Repeat
Signal
Transmembrane
Transmembrane helix
   Molecular functionReceptor
   PTMGlycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processI-kappaB phosphorylation

Inferred from electronic annotation. Source: Compara

MyD88-dependent toll-like receptor signaling pathway

Inferred from electronic annotation. Source: InterPro

cellular response to mechanical stimulus

Inferred from electronic annotation. Source: Compara

defense response to virus

Inferred from mutant phenotype Ref.2. Source: UniProtKB

inflammatory response

Inferred from electronic annotation. Source: UniProtKB-KW

innate immune response

Inferred from electronic annotation. Source: UniProtKB-KW

microglial cell activation involved in immune response

Inferred from electronic annotation. Source: Compara

pathogen-associated molecular pattern dependent induction by symbiont of host innate immune response

Inferred from electronic annotation. Source: Compara

positive regulation of NF-kappaB import into nucleus

Inferred from electronic annotation. Source: Compara

positive regulation of chemokine production

Inferred from electronic annotation. Source: Compara

positive regulation of interferon-alpha biosynthetic process

Inferred from mutant phenotype Ref.2. Source: UniProtKB

positive regulation of interferon-beta biosynthetic process

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of interferon-gamma biosynthetic process

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of interleukin-6 production

Inferred from direct assay PubMed 18261938. Source: MGI

positive regulation of interleukin-8 biosynthetic process

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of interleukin-8 production

Inferred from electronic annotation. Source: Compara

response to chemical stimulus

Inferred from electronic annotation. Source: Compara

toll-like receptor 7 signaling pathway

Inferred from mutant phenotype Ref.2. Source: UniProtKB

   Cellular_componentearly phagosome

Inferred from direct assay Ref.5. Source: UniProtKB

endoplasmic reticulum

Inferred from direct assay Ref.5. Source: UniProtKB

endoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

endosome

Inferred from direct assay Ref.5. Source: UniProtKB

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

lysosome

Inferred from direct assay Ref.5. Source: UniProtKB

plasma membrane

Inferred from electronic annotation. Source: Compara

   Molecular_functiondouble-stranded RNA binding

Inferred from sequence or structural similarity. Source: UniProtKB

drug binding

Inferred from electronic annotation. Source: Compara

siRNA binding

Inferred from mutant phenotype PubMed 15723075. Source: UniProtKB

single-stranded RNA binding

Inferred from mutant phenotype PubMed 14976262. Source: UniProtKB

transmembrane signaling receptor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Chain27 – 10501024Toll-like receptor 7
PRO_0000034734

Regions

Topological domain27 – 837811Extracellular Potential
Transmembrane838 – 85821Helical; Potential
Topological domain859 – 1050192Cytoplasmic Potential
Repeat42 – 6423LRR 1
Repeat65 – 8723LRR 2
Repeat89 – 11123LRR 3
Repeat126 – 14924LRR 4
Repeat151 – 17020LRR 5
Repeat171 – 19525LRR 6
Repeat203 – 22624LRR 7
Repeat228 – 24720LRR 8
Repeat248 – 27326LRR 9
Repeat275 – 28915LRR 10
Repeat290 – 31223LRR 11
Repeat314 – 33724LRR 12
Repeat339 – 36426LRR 13
Repeat369 – 39224LRR 14
Repeat396 – 41924LRR 15
Repeat421 – 44323LRR 16
Repeat493 – 51624LRR 17
Repeat517 – 54226LRR 18
Repeat543 – 56523LRR 19
Repeat567 – 58923LRR 20
Repeat596 – 61924LRR 21
Repeat620 – 64526LRR 22
Repeat650 – 67324LRR 23
Repeat675 – 69824LRR 24
Repeat699 – 72224LRR 25
Repeat724 – 74623LRR 26
Repeat747 – 77024LRR 27
Repeat773 – 79624LRR 28
Domain890 – 1037148TIR

Amino acid modifications

Modified residue10111Phosphoserine Ref.4
Glycosylation661N-linked (GlcNAc...) Potential
Glycosylation691N-linked (GlcNAc...) Potential
Glycosylation1671N-linked (GlcNAc...) Potential
Glycosylation1901N-linked (GlcNAc...) Potential
Glycosylation2151N-linked (GlcNAc...) Potential
Glycosylation3871N-linked (GlcNAc...) Potential
Glycosylation5241N-linked (GlcNAc...) Potential
Glycosylation5351N-linked (GlcNAc...) Potential
Glycosylation5911N-linked (GlcNAc...) Potential
Glycosylation6801N-linked (GlcNAc...) Potential
Glycosylation7211N-linked (GlcNAc...) Potential
Glycosylation8001N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P58681 [UniParc].

Last modified January 31, 2002. Version 1.
Checksum: 495B75DEE849D8EE

FASTA1,050121,837
        10         20         30         40         50         60 
MVFSMWTRKR QILIFLNMLL VSRVFGFRWF PKTLPCEVKV NIPEAHVIVD CTDKHLTEIP 

        70         80         90        100        110        120 
EGIPTNTTNL TLTINHIPSI SPDSFRRLNH LEEIDLRCNC VPVLLGSKAN VCTKRLQIRP 

       130        140        150        160        170        180 
GSFSGLSDLK ALYLDGNQLL EIPQDLPSSL HLLSLEANNI FSITKENLTE LVNIETLYLG 

       190        200        210        220        230        240 
QNCYYRNPCN VSYSIEKDAF LVMRNLKVLS LKDNNVTAVP TTLPPNLLEL YLYNNIIKKI 

       250        260        270        280        290        300 
QENDFNNLNE LQVLDLSGNC PRCYNVPYPC TPCENNSPLQ IHDNAFNSLT ELKVLRLHSN 

       310        320        330        340        350        360 
SLQHVPPTWF KNMRNLQELD LSQNYLAREI EEAKFLHFLP NLVELDFSFN YELQVYHASI 

       370        380        390        400        410        420 
TLPHSLSSLE NLKILRVKGY VFKELKNSSL SVLHKLPRLE VLDLGTNFIK IADLNIFKHF 

       430        440        450        460        470        480 
ENLKLIDLSV NKISPSEESR EVGFCPNAQT SVDRHGPQVL EALHYFRYDE YARSCRFKNK 

       490        500        510        520        530        540 
EPPSFLPLNA DCHIYGQTLD LSRNNIFFIK PSDFQHLSFL KCLNLSGNTI GQTLNGSELW 

       550        560        570        580        590        600 
PLRELRYLDF SNNRLDLLYS TAFEELQSLE VLDLSSNSHY FQAEGITHML NFTKKLRLLD 

       610        620        630        640        650        660 
KLMMNDNDIS TSASRTMESD SLRILEFRGN HLDVLWRAGD NRYLDFFKNL FNLEVLDISR 

       670        680        690        700        710        720 
NSLNSLPPEV FEGMPPNLKN LSLAKNGLKS FFWDRLQLLK HLEILDLSHN QLTKVPERLA 

       730        740        750        760        770        780 
NCSKSLTTLI LKHNQIRQLT KYFLEDALQL RYLDISSNKI QVIQKTSFPE NVLNNLEMLV 

       790        800        810        820        830        840 
LHHNRFLCNC DAVWFVWWVN HTDVTIPYLA TDVTCVGPGA HKGQSVISLD LYTCELDLTN 

       850        860        870        880        890        900 
LILFSVSISS VLFLMVVMTT SHLFFWDMWY IYYFWKAKIK GYQHLQSMES CYDAFIVYDT 

       910        920        930        940        950        960 
KNSAVTEWVL QELVAKLEDP REKHFNLCLE ERDWLPGQPV LENLSQSIQL SKKTVFVMTQ 

       970        980        990       1000       1010       1020 
KYAKTESFKM AFYLSHQRLL DEKVDVIILI FLEKPLQKSK FLQLRKRLCR SSVLEWPANP 

      1030       1040       1050 
QAHPYFWQCL KNALTTDNHV AYSQMFKETV

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of murine Toll-like receptor 7."
Heil F.J., Lipford G.B., Wagner H., Bauer S.M.
Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Macrophage.
[2]"Innate antiviral responses by means of TLR7-mediated recognition of single-stranded RNA."
Diebold S.S., Kaisho T., Hemmi H., Akira S., Reis e Sousa C.
Science 303:1529-1531(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[3]"The interaction between the ER membrane protein UNC93B and TLR3, 7, and 9 is crucial for TLR signaling."
Brinkmann M.M., Spooner E., Hoebe K., Beutler B., Ploegh H.L., Kim Y.M.
J. Cell Biol. 177:265-275(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH UNC93B1.
[4]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1011, MASS SPECTROMETRY.
Tissue: Liver.
[5]"UNC93B1 delivers nucleotide-sensing toll-like receptors to endolysosomes."
Kim Y.M., Brinkmann M.M., Paquet M.E., Ploegh H.L.
Nature 452:234-238(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY035889 mRNA. Translation: AAK62676.1.
IPIIPI00122181.
RefSeqNP_573474.1. NM_133211.3.
UniGeneMm.23979.

3D structure databases

ProteinModelPortalP58681.
SMRP58681. Positions 46-835, 892-1036.
ModBaseSearch...

PTM databases

PhosphoSiteP58681.

Proteomic databases

PaxDbP58681.
PRIDEP58681.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000060719; ENSMUSP00000061853; ENSMUSG00000044583.
ENSMUST00000112161; ENSMUSP00000107787; ENSMUSG00000044583.
ENSMUST00000112164; ENSMUSP00000107789; ENSMUSG00000044583.
GeneID170743.
KEGGmmu:170743.
UCSCuc009uwz.1. mouse.

Organism-specific databases

CTD51284.
MGIMGI:2176882. Tlr7.

Phylogenomic databases

eggNOGCOG4886.
HOGENOMHOG000230468.
HOVERGENHBG018601.
InParanoidP58681.
KOK05404.
OrthoDBEOG4BCDM5.

Gene expression databases

ArrayExpressP58681.
BgeeP58681.
CleanExMM_TLR7.
GenevestigatorP58681.
GermOnlineENSMUSG00000044583. Mus musculus.

Family and domain databases

InterProIPR000483. Cys-rich_flank_reg_C.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000372. LRR-contain_N.
IPR000157. TIR_dom.
IPR026880. TLR7.
[Graphical view]
PANTHERPTHR24365:SF1. PTHR24365:SF1. 1 hit.
PfamPF00560. LRR_1. 2 hits.
PF01582. TIR. 1 hit.
[Graphical view]
SMARTSM00369. LRR_TYP. 3 hits.
SM00082. LRRCT. 1 hit.
SM00013. LRRNT. 1 hit.
[Graphical view]
SUPFAMSSF52200. TIR. 1 hit.
PROSITEPS51450. LRR. 19 hits.
PS50104. TIR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChEMBLCHEMBL6085.
NextBio370306.
SOURCESearch...

Entry information

Entry nameTLR7_MOUSE
AccessionPrimary (citable) accession number: P58681
Secondary accession number(s): Q923I1
Entry history
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: January 31, 2002
Last modified: May 29, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents