Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Toll-like receptor 7

Gene

Tlr7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.1 Publication

GO - Molecular functioni

  1. double-stranded RNA binding Source: UniProtKB
  2. drug binding Source: Ensembl
  3. single-stranded RNA binding Source: UniProtKB
  4. siRNA binding Source: UniProtKB
  5. transmembrane signaling receptor activity Source: InterPro

GO - Biological processi

  1. cellular response to mechanical stimulus Source: Ensembl
  2. defense response to virus Source: UniProtKB
  3. I-kappaB phosphorylation Source: MGI
  4. inflammatory response Source: UniProtKB-KW
  5. innate immune response Source: UniProtKB-KW
  6. microglial cell activation Source: Ensembl
  7. MyD88-dependent toll-like receptor signaling pathway Source: InterPro
  8. positive regulation of chemokine production Source: MGI
  9. positive regulation of interferon-alpha biosynthetic process Source: UniProtKB
  10. positive regulation of interferon-beta biosynthetic process Source: UniProtKB
  11. positive regulation of interferon-gamma biosynthetic process Source: UniProtKB
  12. positive regulation of interleukin-6 production Source: MGI
  13. positive regulation of interleukin-8 biosynthetic process Source: UniProtKB
  14. positive regulation of interleukin-8 production Source: MGI
  15. positive regulation of NF-kappaB import into nucleus Source: MGI
  16. regulation of protein phosphorylation Source: MGI
  17. toll-like receptor 7 signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Immunity, Inflammatory response, Innate immunity

Enzyme and pathway databases

ReactomeiREACT_271614. TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation.
REACT_297122. TRAF6 mediated IRF7 activation in TLR7/8 or 9 signaling.
REACT_338311. Trafficking and processing of endosomal TLR.
REACT_340382. Toll Like Receptor 7/8 (TLR7/8) Cascade.
REACT_348851. MyD88 dependent cascade initiated on endosome.

Names & Taxonomyi

Protein namesi
Recommended name:
Toll-like receptor 7
Gene namesi
Name:Tlr7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:2176882. Tlr7.

Subcellular locationi

Endoplasmic reticulum membrane 1 Publication; Single-pass type I membrane protein 1 Publication. Endosome 1 Publication. Lysosome 1 Publication. Cytoplasmic vesiclephagosome 1 Publication
Note: Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini27 – 837811ExtracellularSequence AnalysisAdd
BLAST
Transmembranei838 – 85821HelicalSequence AnalysisAdd
BLAST
Topological domaini859 – 1050192CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. early phagosome Source: UniProtKB
  3. endoplasmic reticulum Source: UniProtKB
  4. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  5. endosome Source: UniProtKB
  6. integral component of membrane Source: UniProtKB-KW
  7. lysosome Source: UniProtKB
  8. plasma membrane Source: MGI
  9. receptor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence AnalysisAdd
BLAST
Chaini27 – 10501024Toll-like receptor 7PRO_0000034734Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi66 – 661N-linked (GlcNAc...)Sequence Analysis
Glycosylationi69 – 691N-linked (GlcNAc...)Sequence Analysis
Glycosylationi167 – 1671N-linked (GlcNAc...)Sequence Analysis
Glycosylationi190 – 1901N-linked (GlcNAc...)Sequence Analysis
Glycosylationi215 – 2151N-linked (GlcNAc...)Sequence Analysis
Glycosylationi387 – 3871N-linked (GlcNAc...)Sequence Analysis
Glycosylationi524 – 5241N-linked (GlcNAc...)Sequence Analysis
Glycosylationi535 – 5351N-linked (GlcNAc...)Sequence Analysis
Glycosylationi591 – 5911N-linked (GlcNAc...)Sequence Analysis
Glycosylationi680 – 6801N-linked (GlcNAc...)Sequence Analysis
Glycosylationi721 – 7211N-linked (GlcNAc...)Sequence Analysis
Glycosylationi800 – 8001N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP58681.
PRIDEiP58681.

PTM databases

PhosphoSiteiP58681.

Expressioni

Gene expression databases

BgeeiP58681.
CleanExiMM_TLR7.
ExpressionAtlasiP58681. baseline and differential.
GenevestigatoriP58681.

Interactioni

Subunit structurei

Interacts with MYD88 via their respective TIR domains (By similarity). Interacts with UNC93B1.By similarity1 Publication

Structurei

3D structure databases

ProteinModelPortaliP58681.
SMRiP58681. Positions 32-835, 892-1036.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati42 – 6423LRR 1Add
BLAST
Repeati65 – 8723LRR 2Add
BLAST
Repeati89 – 11123LRR 3Add
BLAST
Repeati126 – 14924LRR 4Add
BLAST
Repeati151 – 17020LRR 5Add
BLAST
Repeati171 – 19525LRR 6Add
BLAST
Repeati203 – 22624LRR 7Add
BLAST
Repeati228 – 24720LRR 8Add
BLAST
Repeati248 – 27326LRR 9Add
BLAST
Repeati275 – 28915LRR 10Add
BLAST
Repeati290 – 31223LRR 11Add
BLAST
Repeati314 – 33724LRR 12Add
BLAST
Repeati339 – 36426LRR 13Add
BLAST
Repeati369 – 39224LRR 14Add
BLAST
Repeati396 – 41924LRR 15Add
BLAST
Repeati421 – 44323LRR 16Add
BLAST
Repeati493 – 51624LRR 17Add
BLAST
Repeati517 – 54226LRR 18Add
BLAST
Repeati543 – 56523LRR 19Add
BLAST
Repeati567 – 58923LRR 20Add
BLAST
Repeati596 – 61924LRR 21Add
BLAST
Repeati620 – 64526LRR 22Add
BLAST
Repeati650 – 67324LRR 23Add
BLAST
Repeati675 – 69824LRR 24Add
BLAST
Repeati699 – 72224LRR 25Add
BLAST
Repeati724 – 74623LRR 26Add
BLAST
Repeati747 – 77024LRR 27Add
BLAST
Repeati773 – 79624LRR 28Add
BLAST
Domaini890 – 1037148TIRPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the Toll-like receptor family.Curated
Contains 28 LRR (leucine-rich) repeats.Curated
Contains 1 TIR domain.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG4886.
HOGENOMiHOG000230468.
HOVERGENiHBG018601.
InParanoidiP58681.
KOiK05404.
OMAiAFYLSHQ.
OrthoDBiEOG7VB2DM.
PhylomeDBiP58681.
TreeFamiTF325595.

Family and domain databases

Gene3Di3.40.50.10140. 1 hit.
InterProiIPR000483. Cys-rich_flank_reg_C.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000372. LRR-contain_N.
IPR000157. TIR_dom.
IPR026880. TLR7.
[Graphical view]
PANTHERiPTHR24365:SF222. PTHR24365:SF222. 1 hit.
PfamiPF00560. LRR_1. 2 hits.
PF13855. LRR_8. 4 hits.
PF01582. TIR. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 3 hits.
SM00082. LRRCT. 1 hit.
SM00013. LRRNT. 1 hit.
[Graphical view]
SUPFAMiSSF52200. SSF52200. 1 hit.
PROSITEiPS51450. LRR. 19 hits.
PS50104. TIR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P58681-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVFSMWTRKR QILIFLNMLL VSRVFGFRWF PKTLPCEVKV NIPEAHVIVD
60 70 80 90 100
CTDKHLTEIP EGIPTNTTNL TLTINHIPSI SPDSFRRLNH LEEIDLRCNC
110 120 130 140 150
VPVLLGSKAN VCTKRLQIRP GSFSGLSDLK ALYLDGNQLL EIPQDLPSSL
160 170 180 190 200
HLLSLEANNI FSITKENLTE LVNIETLYLG QNCYYRNPCN VSYSIEKDAF
210 220 230 240 250
LVMRNLKVLS LKDNNVTAVP TTLPPNLLEL YLYNNIIKKI QENDFNNLNE
260 270 280 290 300
LQVLDLSGNC PRCYNVPYPC TPCENNSPLQ IHDNAFNSLT ELKVLRLHSN
310 320 330 340 350
SLQHVPPTWF KNMRNLQELD LSQNYLAREI EEAKFLHFLP NLVELDFSFN
360 370 380 390 400
YELQVYHASI TLPHSLSSLE NLKILRVKGY VFKELKNSSL SVLHKLPRLE
410 420 430 440 450
VLDLGTNFIK IADLNIFKHF ENLKLIDLSV NKISPSEESR EVGFCPNAQT
460 470 480 490 500
SVDRHGPQVL EALHYFRYDE YARSCRFKNK EPPSFLPLNA DCHIYGQTLD
510 520 530 540 550
LSRNNIFFIK PSDFQHLSFL KCLNLSGNTI GQTLNGSELW PLRELRYLDF
560 570 580 590 600
SNNRLDLLYS TAFEELQSLE VLDLSSNSHY FQAEGITHML NFTKKLRLLD
610 620 630 640 650
KLMMNDNDIS TSASRTMESD SLRILEFRGN HLDVLWRAGD NRYLDFFKNL
660 670 680 690 700
FNLEVLDISR NSLNSLPPEV FEGMPPNLKN LSLAKNGLKS FFWDRLQLLK
710 720 730 740 750
HLEILDLSHN QLTKVPERLA NCSKSLTTLI LKHNQIRQLT KYFLEDALQL
760 770 780 790 800
RYLDISSNKI QVIQKTSFPE NVLNNLEMLV LHHNRFLCNC DAVWFVWWVN
810 820 830 840 850
HTDVTIPYLA TDVTCVGPGA HKGQSVISLD LYTCELDLTN LILFSVSISS
860 870 880 890 900
VLFLMVVMTT SHLFFWDMWY IYYFWKAKIK GYQHLQSMES CYDAFIVYDT
910 920 930 940 950
KNSAVTEWVL QELVAKLEDP REKHFNLCLE ERDWLPGQPV LENLSQSIQL
960 970 980 990 1000
SKKTVFVMTQ KYAKTESFKM AFYLSHQRLL DEKVDVIILI FLEKPLQKSK
1010 1020 1030 1040 1050
FLQLRKRLCR SSVLEWPANP QAHPYFWQCL KNALTTDNHV AYSQMFKETV
Length:1,050
Mass (Da):121,837
Last modified:January 31, 2002 - v1
Checksum:i495B75DEE849D8EE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY035889 mRNA. Translation: AAK62676.1.
CCDSiCCDS30531.1.
CCDS72469.1.
RefSeqiNP_001277684.1. NM_001290755.1.
NP_001277685.1. NM_001290756.1.
NP_001277686.1. NM_001290757.1.
NP_573474.1. NM_133211.4.
UniGeneiMm.23979.
Mm.489377.

Genome annotation databases

EnsembliENSMUST00000060719; ENSMUSP00000061853; ENSMUSG00000044583.
ENSMUST00000112161; ENSMUSP00000107787; ENSMUSG00000044583.
ENSMUST00000112164; ENSMUSP00000107789; ENSMUSG00000044583.
GeneIDi170743.
KEGGimmu:170743.
UCSCiuc009uwz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY035889 mRNA. Translation: AAK62676.1.
CCDSiCCDS30531.1.
CCDS72469.1.
RefSeqiNP_001277684.1. NM_001290755.1.
NP_001277685.1. NM_001290756.1.
NP_001277686.1. NM_001290757.1.
NP_573474.1. NM_133211.4.
UniGeneiMm.23979.
Mm.489377.

3D structure databases

ProteinModelPortaliP58681.
SMRiP58681. Positions 32-835, 892-1036.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

BindingDBiP58681.
ChEMBLiCHEMBL3137280.

PTM databases

PhosphoSiteiP58681.

Proteomic databases

PaxDbiP58681.
PRIDEiP58681.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000060719; ENSMUSP00000061853; ENSMUSG00000044583.
ENSMUST00000112161; ENSMUSP00000107787; ENSMUSG00000044583.
ENSMUST00000112164; ENSMUSP00000107789; ENSMUSG00000044583.
GeneIDi170743.
KEGGimmu:170743.
UCSCiuc009uwz.1. mouse.

Organism-specific databases

CTDi51284.
MGIiMGI:2176882. Tlr7.

Phylogenomic databases

eggNOGiCOG4886.
HOGENOMiHOG000230468.
HOVERGENiHBG018601.
InParanoidiP58681.
KOiK05404.
OMAiAFYLSHQ.
OrthoDBiEOG7VB2DM.
PhylomeDBiP58681.
TreeFamiTF325595.

Enzyme and pathway databases

ReactomeiREACT_271614. TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation.
REACT_297122. TRAF6 mediated IRF7 activation in TLR7/8 or 9 signaling.
REACT_338311. Trafficking and processing of endosomal TLR.
REACT_340382. Toll Like Receptor 7/8 (TLR7/8) Cascade.
REACT_348851. MyD88 dependent cascade initiated on endosome.

Miscellaneous databases

NextBioi370306.
PROiP58681.
SOURCEiSearch...

Gene expression databases

BgeeiP58681.
CleanExiMM_TLR7.
ExpressionAtlasiP58681. baseline and differential.
GenevestigatoriP58681.

Family and domain databases

Gene3Di3.40.50.10140. 1 hit.
InterProiIPR000483. Cys-rich_flank_reg_C.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000372. LRR-contain_N.
IPR000157. TIR_dom.
IPR026880. TLR7.
[Graphical view]
PANTHERiPTHR24365:SF222. PTHR24365:SF222. 1 hit.
PfamiPF00560. LRR_1. 2 hits.
PF13855. LRR_8. 4 hits.
PF01582. TIR. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 3 hits.
SM00082. LRRCT. 1 hit.
SM00013. LRRNT. 1 hit.
[Graphical view]
SUPFAMiSSF52200. SSF52200. 1 hit.
PROSITEiPS51450. LRR. 19 hits.
PS50104. TIR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning of murine Toll-like receptor 7."
    Heil F.J., Lipford G.B., Wagner H., Bauer S.M.
    Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Macrophage.
  2. "Innate antiviral responses by means of TLR7-mediated recognition of single-stranded RNA."
    Diebold S.S., Kaisho T., Hemmi H., Akira S., Reis e Sousa C.
    Science 303:1529-1531(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  3. "The interaction between the ER membrane protein UNC93B and TLR3, 7, and 9 is crucial for TLR signaling."
    Brinkmann M.M., Spooner E., Hoebe K., Beutler B., Ploegh H.L., Kim Y.M.
    J. Cell Biol. 177:265-275(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH UNC93B1.
  4. "UNC93B1 delivers nucleotide-sensing toll-like receptors to endolysosomes."
    Kim Y.M., Brinkmann M.M., Paquet M.E., Ploegh H.L.
    Nature 452:234-238(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiTLR7_MOUSE
AccessioniPrimary (citable) accession number: P58681
Secondary accession number(s): Q923I1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: January 31, 2002
Last modified: April 1, 2015
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.