Reviewed, UniProtKB/Swiss-Prot P58674 (ISPH_ANASP)

Names and origin

Protein names

Recommended name:
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
    EC=1.17.1.2

Gene names

Name:	ispH
Synonyms:	lytB
Ordered Locus Names:	all0985

Organism

Anabaena sp. (strain PCC 7120) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	402 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity.
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity.
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the ispH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 402	402	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191		PRO_0000128765

Sequences

	Sequence		Length	Mass (Da)
	P58674-1 [UniParc]. Last modified January 31, 2002. Version 1. Checksum: 4DDC7B5FFF7B4DFA	FASTA	402	45,385
	10 20 30 40 50 60 MDTKTFKRTL QHSENYNRKG FGHQAEVATQ LQSEYQSSLI QEIRDRNYTL QRGDVTIRLA 70 80 90 100 110 120 QAFGFCWGVE RAVAMAYETR KHFPTERIWI TNEIIHNPSV NQRMQEMQVG FIPVEAGNKD 130 140 150 160 170 180 FSVVGNNDVV ILPAFGASVQ EMQLLSEKGC KIVDTTCPWV SKVWNTVEKH KKGDHTSIIH 190 200 210 220 230 240 GKYKHEETIA TSSFAGKYLI VLNLKEAQYV ADYILHGGNR EEFLQKFAKA CSAGFDPDRD 250 260 270 280 290 300 LERVGIANQT TMLKGETEQI GKLFEHTMLQ KYGPVELNQH FQSFNTICDA TQERQDAMLE 310 320 330 340 350 360 LVQENLDLMI VIGGFNSSNT TQLQQISQER GLPSYHIDVV ERIKSINSIE HRQLNGELVT 370 380 390 400 TENWLPAGKI VVGVTSGAST PDKVVEDVIE KIFALKATAA VF

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000019 Genomic DNA. Translation: BAB72942.1.
PIR	AF1929.
RefSeq	NP_485028.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P58674.
Genome annotation databases
GeneID	1104579.
GenomeReviews	Gene locus all0985 in contig BA000019_GR.
KEGG	ana:all0985.
NMPDR	fig|103690.1.peg.1295.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P58674.
OMA	KNDICYA.
Enzyme and pathway databases
BioCyc	NSP103690:ALL0985-MON.
Family and domain databases
HAMAP	MF_00191. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. ispH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH_ANASP

Accession

Primary (citable) accession number: P58674

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 31, 2002
Last sequence update:	January 31, 2002
Last modified:	November 3, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)