Reviewed, UniProtKB/Swiss-Prot P58601 (PME1_RALSO)

Names and origin

Protein names

Recommended name:
    Pectinesterase
      Short name=PE
    EC=3.1.1.11
Alternative name(s):
    Pectin methylesterase

Gene names

Name:	pme
Ordered Locus Names:	RSp0138
ORF Names:	RS02982

Encoded on

Plasmid megaplasmid Rsp

Organism

Ralstonia solanacearum (Pseudomonas solanacearum) [Complete proteome] [HAMAP]

Taxonomic identifier

305 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in maceration and soft-rotting of plant tissue By similarity.
Catalytic activity	Pectin + n H2O = n methanol + pectate.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the pectinesterase family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Aspartyl esterase Hydrolase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	cell wall modification Inferred from electronic annotation. Source: InterPro
Cellular component	cell wall Inferred from electronic annotation. Source: InterPro extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aspartyl esterase activity Inferred from electronic annotation. Source: UniProtKB-KW pectinesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 396	375	Pectinesterase		PRO_0000023500
Sites
	Active site	232	1	Proton donor By similarity
	Active site	259	1	Nucleophile By similarity
	Binding site	174	1	Substrate By similarity
	Binding site	324	1	Substrate By similarity
	Binding site	326	1	Substrate By similarity
	Site	231	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	P58601-1 [UniParc]. Last modified January 23, 2002. Version 1. Checksum: 2CAAD93DFE8D5D60	FASTA	396	40,961
	10 20 30 40 50 60 MQSKTLYLKA TALLGGCTVF AATALAVTST ATRPQLSSAD ARTYTIAKYL ASFGTIGSLT 70 80 90 100 110 120 TDNWDPTGGV GAVSGFRANY AVAADGTAQY KTVQAAIDAA VNAGGVARRY ISVKAGIYNE 130 140 150 160 170 180 LVCVPESAPP ITLYGLDANA GNTQIVYNNA NPTPASGAKT NPCMGTSSNA TVGTLRSATA 190 200 210 220 230 240 MVRASDFHAR NLTFKNSYVE GTYADNNQSA VALAVRGDKA ILENVSVIGN QDTLFVGATS 250 260 270 280 290 300 TTTVIRAYFK NSFIQGDTDF IFGAGTAVFH GCTIQYTAAR LGAKAASYIF APSTAPGNPY 310 320 330 340 350 360 GFLAINSTFN ATGNPPNNSL HLGRAWDQSV SGTSAYINGS SPNGQVVIRD SSLGALIRLA 370 380 390 DPWGPSTAGR PYCSANCAYS ANRFFEYNNT GAGSGN

References

[1]

"Genome sequence of the plant pathogen Ralstonia solanacearum." Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M., Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M., Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M., Moisan A. , Robert C., Saurin W., Schiex T., Siguier P., Thebault P., Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A. Nature 415:497-502(2002) [PubMed: 11823852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GMI1000.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL646053 Genomic DNA. Translation: CAD17289.1. Different initiation.
3D structure databases
HSSP	HSSP built from PDB template 1GQ8 based on UniProtKB P83218.
ModBase	Search...
Genome annotation databases
GenomeReviews	Gene locus RSp0138 in contig AL646053_GR.
KEGG	rso:RSp0138.
NMPDR	fig|267608.1.peg.3578.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P58601.
Enzyme and pathway databases
BioCyc	RSOL267608:RSP0138-MON.
BRENDA	3.1.1.11. 97066.
Family and domain databases
InterPro	IPR012334. Pectin_lyas_fold. IPR018040. Pectinesterase_AS. IPR000070. Pectinesterase_cat. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF01095. Pectinesterase. 1 hit. [Graphical view]
PROSITE	PS00800. PECTINESTERASE_1. False negative. PS00503. PECTINESTERASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PME1_RALSO

Accession

Primary (citable) accession number: P58601

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	January 23, 2002
Last modified:	September 22, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)