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Protein

Endoglucanase

Gene

egl

Organism
Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei247 – 2471Proton donorBy similarity
Active sitei359 – 3591NucleophileBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciRSOL267608:GCVU-3665-MONOMER.

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase (EC:3.2.1.4)
Alternative name(s):
Cellulase
Endo-1,4-beta-glucanase
Gene namesi
Name:egl
Ordered Locus Names:RSp0162
ORF Names:RS05516
Encoded oniPlasmid megaplasmid Rsp0 Publication
OrganismiRalstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Taxonomic identifieri267608 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
ProteomesiUP000001436 Componenti: Plasmid megaplasmid Rsp

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919PROSITE-ProRule annotationAdd
BLAST
Propeptidei20 – 4324By similarityPRO_0000007867Add
BLAST
Chaini44 – 424381EndoglucanasePRO_0000007868Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi20 – 201N-palmitoyl cysteinePROSITE-ProRule annotation
Lipidationi20 – 201S-diacylglycerol cysteinePROSITE-ProRule annotation

Keywords - PTMi

Lipoprotein, Palmitate, Zymogen

Structurei

3D structure databases

ProteinModelPortaliP58599.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2730.
HOGENOMiHOG000111120.
KOiK01179.
OMAiWTYWAAG.
OrthoDBiEOG6677NC.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00659. GLYCOSYL_HYDROL_F5. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P58599-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHRCMPLVAA SMAALMLAGC GGGDGDTTLS TAAATDTTTL KTAATTSISP
60 70 80 90 100
LWLTIAKDSA AFTVSGTRTV RYGAGSAWVA KSMSGTGQCT AAFFGKDPAA
110 120 130 140 150
GVAKVCQVAQ GTGTLLWRGV SLAGAEFGEG SLPGTYGSNY IYPSADSATY
160 170 180 190 200
YKNKGMNLVR LPFRWERLQP TLNQALDANE LSRLTGFVNA VTAAGQTVLL
210 220 230 240 250
DPHNYARYYG NVIGSSAVPN SAYADFWRRV ATQFKGNARV IFGLMNEPNS
260 270 280 290 300
MPTEQWLSGA NAALAAIRSA NASNVVFVPG NAWTGAHSWN QNWYGTPNGT
310 320 330 340 350
VMKGINDPGR NLVFEVHQYL DGDSSGQSAS CVSATIGAER LQDFTNWLRS
360 370 380 390 400
NGYRGFLGEF GAASNDTCNQ AVANMLTFVK NNADVWTGWA WWAGGPWWGG
410 420
YMYSIEPSNG VDKPQMSVLA PYLK
Length:424
Mass (Da):45,081
Last modified:January 23, 2002 - v1
Checksum:i5BF0D161AB7C87EF
GO

Sequence cautioni

The sequence CAD17313.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL646053 Genomic DNA. Translation: CAD17313.1. Different initiation.
RefSeqiNP_521723.1. NC_003296.1.

Genome annotation databases

EnsemblBacteriaiCAD17313; CAD17313; RSp0162.
KEGGirso:RSp0162.
PATRICi20265911. VBIRalSol70888_3628.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL646053 Genomic DNA. Translation: CAD17313.1. Different initiation.
RefSeqiNP_521723.1. NC_003296.1.

3D structure databases

ProteinModelPortaliP58599.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAD17313; CAD17313; RSp0162.
KEGGirso:RSp0162.
PATRICi20265911. VBIRalSol70888_3628.

Phylogenomic databases

eggNOGiCOG2730.
HOGENOMiHOG000111120.
KOiK01179.
OMAiWTYWAAG.
OrthoDBiEOG6677NC.

Enzyme and pathway databases

BioCyciRSOL267608:GCVU-3665-MONOMER.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00659. GLYCOSYL_HYDROL_F5. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GMI1000.

Entry informationi

Entry nameiGUN_RALSO
AccessioniPrimary (citable) accession number: P58599
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: January 23, 2002
Last modified: June 24, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.