P58599 (GUN_RALSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase
EC=3.2.1.4

Alternative name(s):
Cellulase
Endo-1,4-beta-glucanase

Gene names

Name:	egl
Ordered Locus Names:	RSp0162
ORF Names:	RS05516

Encoded on

Plasmid megaplasmid Rsp

Organism

Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum) [Complete proteome] [HAMAP]

Taxonomic identifier

267608 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia › Ralstonia solanacearum

Protein attributes

Sequence length	424 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Cell membrane; Lipid-anchor Probable.
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family.
Sequence caution	The sequence CAD17313.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Cellular component	Cell membrane Membrane
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Lipoprotein Palmitate Zymogen
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	By similarity
	Propeptide	20 – 43	24	By similarity		PRO_0000007867
	Chain	44 – 424	381	Endoglucanase		PRO_0000007868
Sites
	Active site	247	1	Proton donor By similarity
	Active site	359	1	Nucleophile By similarity
Amino acid modifications
	Lipidation	20	1	N-palmitoyl cysteine By similarity
	Lipidation	20	1	S-diacylglycerol cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P58599 [UniParc]. Last modified January 23, 2002. Version 1. Checksum: 5BF0D161AB7C87EF	FASTA	424	45,081
	10 20 30 40 50 60 MHRCMPLVAA SMAALMLAGC GGGDGDTTLS TAAATDTTTL KTAATTSISP LWLTIAKDSA 70 80 90 100 110 120 AFTVSGTRTV RYGAGSAWVA KSMSGTGQCT AAFFGKDPAA GVAKVCQVAQ GTGTLLWRGV 130 140 150 160 170 180 SLAGAEFGEG SLPGTYGSNY IYPSADSATY YKNKGMNLVR LPFRWERLQP TLNQALDANE 190 200 210 220 230 240 LSRLTGFVNA VTAAGQTVLL DPHNYARYYG NVIGSSAVPN SAYADFWRRV ATQFKGNARV 250 260 270 280 290 300 IFGLMNEPNS MPTEQWLSGA NAALAAIRSA NASNVVFVPG NAWTGAHSWN QNWYGTPNGT 310 320 330 340 350 360 VMKGINDPGR NLVFEVHQYL DGDSSGQSAS CVSATIGAER LQDFTNWLRS NGYRGFLGEF 370 380 390 400 410 420 GAASNDTCNQ AVANMLTFVK NNADVWTGWA WWAGGPWWGG YMYSIEPSNG VDKPQMSVLA PYLK

References

[1]

"Genome sequence of the plant pathogen Ralstonia solanacearum." Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M., Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M., Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M., Moisan A. , Robert C., Saurin W., Schiex T., Siguier P., Thebault P., Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A. Nature 415:497-502(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GMI1000.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL646053 Genomic DNA. Translation: CAD17313.1. Different initiation.
RefSeq	NP_521723.1. NC_003296.1.
3D structure databases
ProteinModelPortal	P58599.
ModBase	Search...
Protein-protein interaction databases
STRING	267608.RSp0162.
Protein family/group databases
CAZy	GH5. Glycoside Hydrolase Family 5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAD17313; CAD17313; RSp0162.
GeneID	1222565.
KEGG	rso:RSp0162.
PATRIC	20265911. VBIRalSol70888_3628.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2730.
HOGENOM	HOG000111120.
KO	K01179.
OMA	RWERLQP.
ProtClustDB	CLSK2753836.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR001547. Glyco_hydro_5. IPR018087. Glyco_hydro_5_CS. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00150. Cellulase. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00659. GLYCOSYL_HYDROL_F5. 1 hit. PS51257. PROKAR_LIPOPROTEIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN_RALSO

Accession

Primary (citable) accession number: P58599

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	January 23, 2002
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program