P58598 (PGLR_RALSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Polygalacturonase
Short name=PGA
EC=3.2.1.15

Alternative name(s):
Pectinase

Gene names

Name:	pglA
Ordered Locus Names:	RSp0880
ORF Names:	RS01651

Encoded on

Plasmid megaplasmid Rsp

Organism

Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum) [Complete proteome] [HAMAP]

Taxonomic identifier

267608 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

Protein attributes

Sequence length	531 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Contributes to the wilt disease production on tomato.
Catalytic activity	Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
Subunit structure	Monomer By similarity.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 28 family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	polygalacturonase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 531	508	Polygalacturonase		PRO_0000024760
Regions
	Region	518 – 531	14	Required for PGA export across the outer membrane and catalytic activity By similarity
Sites
	Active site	307	1	Proton donor By similarity
	Active site	333	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P58598 [UniParc]. Last modified January 23, 2002. Version 1. Checksum: E6B8628F3890E281	FASTA	531	54,773
	10 20 30 40 50 60 MILSHRYTLI ALAAAILSSG AHAAGASVTA SWGNVAEPSL PADSAVCKTL AATITPVSGS 70 80 90 100 110 120 IDTVDGNPSN SQPDASRIQT AIDNCPAGQA VRLVKGSAGE SGFLSGSLKL KSGVTLWIDT 130 140 150 160 170 180 GVTLFASRNP ADFDNGVGTC GTATTSNTKS CNALIVARDT VGSGIVGDGI IDGRGGSLVT 190 200 210 220 230 240 SGPNANRLTW WDIAYLNKTK GLNQQNPRLV QLYNGSAFTL YRVTVQNSPN FHIVTTGTAG 250 260 270 280 290 300 VTAWGIKIVT PSLAYTVPGY KCAAGTTPDK VTPATCFTPE TVKNTDGFDP GQSTNVVLAN 310 320 330 340 350 360 SYISTGDDHV AIKASGGATR NLLFAHNHFY YGHGLSIGSE TDGGVSNMQV TDLAMDGNDS 370 380 390 400 410 420 SGGNGLRIKS DISRGGKVNN IVYNGICMRN VKEPLVFDPF YSTAKGSLYP NFTNIVVKNF 430 440 450 460 470 480 HDLGSAKGIA RTMTFLGYEA KKRTNPLTLT LDNVVFDGTQ PAFDVAHNGG PASPNGTHFT 490 500 510 520 530 FGGNGPVSFA NAIVTSSTTD VTVTGTPGTA AAVDCSNAFV PLKSVAPTSP I

References

[1]

"Genome sequence of the plant pathogen Ralstonia solanacearum." Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M., Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M., Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M., Moisan A. , Robert C., Saurin W., Schiex T., Siguier P., Thebault P., Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A. Nature 415:497-502(2002) [PubMed: 11823852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GMI1000.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL646053 Genomic DNA. Translation: CAD18031.1.
RefSeq	NP_522441.1. NC_003296.1.
3D structure databases
ProteinModelPortal	P58598.
ModBase	Search...
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1223187.
GenomeReviews	Gene locus RSp0880 in contig AL646053_GR.
KEGG	rso:RSp0880.
NMPDR	fig|267608.1.peg.4320.
PATRIC	20267357. VBIRalSol70888_4350.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG418213.
OMA	PETVKNT.
ProtClustDB	CLSK481544.
Enzyme and pathway databases
BioCyc	RSOL267608:RSP0880-MONOMER.
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
KO	K01184.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
PROSITE	PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLR_RALSO

Accession

Primary (citable) accession number: P58598

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	January 23, 2002
Last modified:	January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program