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Protein

Glutathione synthetase

Gene

gshB

Organism
Yersinia pestis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg(2+) or Mn2+ ion per subunit.By similarity

Pathway: glutathione biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate--cysteine ligase (gshA)
  2. Glutathione synthetase (gshB)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi281 – 2811Magnesium or manganeseUniRule annotation
Metal bindingi283 – 2831Magnesium or manganeseUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi151 – 20757ATPUniRule annotationAdd
BLAST

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYPES214092:GKDD-919-MONOMER.
UniPathwayiUPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
Alternative name(s):
GSH synthetaseUniRule annotation
Short name:
GSH-SUniRule annotation
Short name:
GSHaseUniRule annotation
Glutathione synthaseUniRule annotation
Gene namesi
Name:gshBUniRule annotation
Ordered Locus Names:YPO0935, y3321, YP_3507
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
ProteomesiUP000000815 Componenti: Chromosome UP000002490 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 319319Glutathione synthetasePRO_0000197500Add
BLAST

Proteomic databases

PRIDEiP58582.

Interactioni

Protein-protein interaction databases

IntActiP58582. 5 interactions.
STRINGi214092.YPO0935.

Structurei

3D structure databases

ProteinModelPortaliP58582.
SMRiP58582. Positions 1-314.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini125 – 311187ATP-graspUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic GSH synthase family.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0189.
HOGENOMiHOG000265022.
KOiK01920.
OMAiMKPLHGH.
OrthoDBiEOG6WMHWB.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_00162. GSH_S.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01380. glut_syn. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P58582-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKLGIVMDP ISSINIKKDT SFAMLLEAQR RGWELHYMEM GDLYMRGGDG
60 70 80 90 100
RARTRRLNVK QDKDNWFSFG AEQDLPLYDL DVILMRKDPP FDTEFIYATY
110 120 130 140 150
ILERAEDKGT LVVNKPQSLR DCNEKLFTAW FPELTPDTLV SRSKDHIRKF
160 170 180 190 200
HQEHGDIILK PLDGMGGTSI FRVKQDDPNL SVIIETLTEL SSRFCMAQNF
210 220 230 240 250
LPAIKEGDKR VLVVDGEPVP YCLARIPAQG ETRGNLAAGG RGEARPLSES
260 270 280 290 300
DWEIARTVAP ILKQKGLIFV GLDIIGDRLT EINVTSPTCV REIEAAFPEI
310
SITGMLMDAI ERRLADKKA
Length:319
Mass (Da):36,015
Last modified:January 23, 2002 - v1
Checksum:i4A26161F2A979AE6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA. Translation: CAL19601.1.
AE009952 Genomic DNA. Translation: AAM86871.1.
AE017042 Genomic DNA. Translation: AAS63661.1.
PIRiAG0114.
RefSeqiNP_670620.1. NC_004088.1.
NP_994784.1. NC_005810.1.
WP_002209976.1. NZ_KN150727.1.
YP_002345982.1. NC_003143.1.

Genome annotation databases

EnsemblBacteriaiAAM86871; AAM86871; y3321.
AAS63661; AAS63661; YP_3507.
GeneIDi1173771.
KEGGiype:YPO0935.
ypk:y3321.
ypm:YP_3507.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA. Translation: CAL19601.1.
AE009952 Genomic DNA. Translation: AAM86871.1.
AE017042 Genomic DNA. Translation: AAS63661.1.
PIRiAG0114.
RefSeqiNP_670620.1. NC_004088.1.
NP_994784.1. NC_005810.1.
WP_002209976.1. NZ_KN150727.1.
YP_002345982.1. NC_003143.1.

3D structure databases

ProteinModelPortaliP58582.
SMRiP58582. Positions 1-314.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP58582. 5 interactions.
STRINGi214092.YPO0935.

Proteomic databases

PRIDEiP58582.

Protocols and materials databases

DNASUi1148268.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM86871; AAM86871; y3321.
AAS63661; AAS63661; YP_3507.
GeneIDi1173771.
KEGGiype:YPO0935.
ypk:y3321.
ypm:YP_3507.

Phylogenomic databases

eggNOGiCOG0189.
HOGENOMiHOG000265022.
KOiK01920.
OMAiMKPLHGH.
OrthoDBiEOG6WMHWB.

Enzyme and pathway databases

UniPathwayiUPA00142; UER00210.
BioCyciYPES214092:GKDD-919-MONOMER.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_00162. GSH_S.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01380. glut_syn. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CO-92 / Biovar Orientalis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KIM10+ / Biovar Mediaevalis.
  3. "Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans."
    Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R.
    , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R.
    DNA Res. 11:179-197(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 91001 / Biovar Mediaevalis.

Entry informationi

Entry nameiGSHB_YERPE
AccessioniPrimary (citable) accession number: P58582
Secondary accession number(s): Q0WIA6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: January 23, 2002
Last modified: June 24, 2015
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.