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Protein

Glutathione synthetase

Gene

gshB

Organism
Yersinia pestis
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg2+ or Mn2+ ion per subunit.By similarity

Pathwayi: glutathione biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate--cysteine ligase (gshA), Glutamate--cysteine ligase (gshA)
  2. Glutathione synthetase (gshB), Glutathione synthetase (gshB)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi281Magnesium or manganeseUniRule annotation1
Metal bindingi283Magnesium or manganeseUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi151 – 207ATPUniRule annotationAdd BLAST57

GO - Molecular functioni

Keywordsi

Molecular functionLigase
Biological processGlutathione biosynthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00142; UER00210

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
Alternative name(s):
GSH synthetaseUniRule annotation
Short name:
GSH-SUniRule annotation
Short name:
GSHaseUniRule annotation
Glutathione synthaseUniRule annotation
Gene namesi
Name:gshBUniRule annotation
Ordered Locus Names:YPO0935, y3321, YP_3507
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeYersinia
Proteomesi
  • UP000000815 Componenti: Chromosome
  • UP000001019 Componenti: Chromosome
  • UP000002490 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001975001 – 319Glutathione synthetaseAdd BLAST319

Proteomic databases

PaxDbiP58582
PRIDEiP58582

Interactioni

Protein-protein interaction databases

IntActiP58582 5 interactors.
STRINGi187410.y3321

Structurei

3D structure databases

ProteinModelPortaliP58582
SMRiP58582
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini125 – 311ATP-graspUniRule annotationAdd BLAST187

Sequence similaritiesi

Belongs to the prokaryotic GSH synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D7Z Bacteria
COG0189 LUCA
HOGENOMiHOG000265022
KOiK01920
OMAiWMRKDPP

Family and domain databases

Gene3Di3.30.1490.201 hit
HAMAPiMF_00162 GSH_S, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR006284 Glut_synth_pro
IPR004218 GSHS_ATP-bd
IPR004215 GSHS_N
IPR016185 PreATP-grasp_dom_sf
PANTHERiPTHR21621:SF4 PTHR21621:SF4, 1 hit
PfamiView protein in Pfam
PF02955 GSH-S_ATP, 1 hit
PF02951 GSH-S_N, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01380 glut_syn, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit

Sequencei

Sequence statusi: Complete.

P58582-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKLGIVMDP ISSINIKKDT SFAMLLEAQR RGWELHYMEM GDLYMRGGDG
60 70 80 90 100
RARTRRLNVK QDKDNWFSFG AEQDLPLYDL DVILMRKDPP FDTEFIYATY
110 120 130 140 150
ILERAEDKGT LVVNKPQSLR DCNEKLFTAW FPELTPDTLV SRSKDHIRKF
160 170 180 190 200
HQEHGDIILK PLDGMGGTSI FRVKQDDPNL SVIIETLTEL SSRFCMAQNF
210 220 230 240 250
LPAIKEGDKR VLVVDGEPVP YCLARIPAQG ETRGNLAAGG RGEARPLSES
260 270 280 290 300
DWEIARTVAP ILKQKGLIFV GLDIIGDRLT EINVTSPTCV REIEAAFPEI
310
SITGMLMDAI ERRLADKKA
Length:319
Mass (Da):36,015
Last modified:January 23, 2002 - v1
Checksum:i4A26161F2A979AE6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA Translation: CAL19601.1
AE009952 Genomic DNA Translation: AAM86871.1
AE017042 Genomic DNA Translation: AAS63661.1
PIRiAG0114
RefSeqiWP_002209976.1, NZ_PDBR01000033.1
YP_002345982.1, NC_003143.1

Genome annotation databases

EnsemblBacteriaiAAM86871; AAM86871; y3321
AAS63661; AAS63661; YP_3507
GeneIDi1173771
KEGGiype:YPO0935
ypk:y3321
ypm:YP_3507
PATRICifig|214092.21.peg.1213

Similar proteinsi

Entry informationi

Entry nameiGSHB_YERPE
AccessioniPrimary (citable) accession number: P58582
Secondary accession number(s): Q0WIA6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: January 23, 2002
Last modified: March 28, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome