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P58581

- GSHB_SALTI

UniProt

P58581 - GSHB_SALTI

Protein

Glutathione synthetase

Gene

gshB

Organism
Salmonella typhi
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (23 Jan 2002)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi281 – 2811Magnesium or manganeseUniRule annotation
    Metal bindingi283 – 2831Magnesium or manganeseUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi151 – 20757ATPUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutathione synthase activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP
    4. manganese ion binding Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Glutathione biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00142; UER00210.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
    Alternative name(s):
    GSH synthetaseUniRule annotation
    Short name:
    GSH-SUniRule annotation
    Short name:
    GSHaseUniRule annotation
    Glutathione synthaseUniRule annotation
    Gene namesi
    Name:gshBUniRule annotation
    Ordered Locus Names:STY3248, t3007
    OrganismiSalmonella typhi
    Taxonomic identifieri90370 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
    ProteomesiUP000000541: Chromosome, UP000002670: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 315315Glutathione synthetasePRO_0000197483Add
    BLAST

    Proteomic databases

    PRIDEiP58581.

    Interactioni

    Protein-protein interaction databases

    STRINGi220341.STY3248.

    Structurei

    Secondary structure

    1
    315
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 75
    Turni11 – 133
    Turni16 – 183
    Helixi20 – 3112
    Beta strandi34 – 385
    Helixi40 – 423
    Beta strandi43 – 464
    Beta strandi49 – 5911
    Beta strandi67 – 7610
    Helixi77 – 793
    Beta strandi81 – 855
    Helixi93 – 10715
    Beta strandi111 – 1144
    Helixi116 – 1216
    Helixi126 – 1349
    Beta strandi138 – 1425
    Helixi144 – 15411
    Beta strandi155 – 1617
    Beta strandi171 – 1733
    Helixi180 – 1889
    Turni189 – 1924
    Beta strandi195 – 1995
    Helixi202 – 2054
    Beta strandi208 – 2147
    Beta strandi220 – 2256
    Beta strandi234 – 2363
    Beta strandi243 – 2464
    Helixi249 – 26517
    Beta strandi269 – 2757
    Beta strandi278 – 2836
    Helixi290 – 2967
    Helixi301 – 31414

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1LP5model-A1-315[»]
    ProteinModelPortaliP58581.
    SMRiP58581. Positions 1-313.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini125 – 310186ATP-graspUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic GSH synthase family.UniRule annotation
    Contains 1 ATP-grasp domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0189.
    HOGENOMiHOG000265022.
    KOiK01920.
    OMAiDWKIARA.
    OrthoDBiEOG6WMHWB.

    Family and domain databases

    Gene3Di3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    HAMAPiMF_00162. GSH_S.
    InterProiIPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR006284. Glut_synth_pro.
    IPR004218. GSHS_ATP-bd.
    IPR004215. GSHS_N.
    IPR016185. PreATP-grasp_dom.
    [Graphical view]
    PfamiPF02955. GSH-S_ATP. 1 hit.
    PF02951. GSH-S_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF52440. SSF52440. 1 hit.
    TIGRFAMsiTIGR01380. glut_syn. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P58581-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIKLGIVMDP IAHINIKKDT SFAMLLEAQR RGYELHYMEM ADLYLINGEA    50
    RACTRTLSVE QNYDKWYEFG SEQEIKLADL DVILMRKDPP FDTEFIYATY 100
    ILERAEEEGT LIVNKPQSLR DCNEKLYTAW FADLTPETLV TRNKAQLKAF 150
    WEKHGDIIMK PLDGMGGASI FRVKEGDPNI GVIAETLTEL GNRYCMAQNY 200
    LPAIKDGDKR VLVVDGEPVP YCLARIPQGG ETRGNLAAGG RGEPRPLSES 250
    DWEIARRVGP TLKAKGLIFV GLDIIGDRLT EINVTSPTCV REIEAEYPIS 300
    ITGMLMDAIE ARLAK 315
    Length:315
    Mass (Da):35,380
    Last modified:January 23, 2002 - v1
    Checksum:iD9AC53F9B23070C3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL513382 Genomic DNA. Translation: CAD02919.1.
    AE014613 Genomic DNA. Translation: AAO70559.1.
    RefSeqiNP_457487.1. NC_003198.1.
    NP_806699.1. NC_004631.1.

    Genome annotation databases

    EnsemblBacteriaiAAO70559; AAO70559; t3007.
    CAD02919; CAD02919; CAD02919.
    GeneIDi1249530.
    KEGGisty:STY3248.
    PATRICi18544572. VBISalEnt120419_3312.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL513382 Genomic DNA. Translation: CAD02919.1 .
    AE014613 Genomic DNA. Translation: AAO70559.1 .
    RefSeqi NP_457487.1. NC_003198.1.
    NP_806699.1. NC_004631.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1LP5 model - A 1-315 [» ]
    ProteinModelPortali P58581.
    SMRi P58581. Positions 1-313.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 220341.STY3248.

    Proteomic databases

    PRIDEi P58581.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO70559 ; AAO70559 ; t3007 .
    CAD02919 ; CAD02919 ; CAD02919 .
    GeneIDi 1249530.
    KEGGi sty:STY3248.
    PATRICi 18544572. VBISalEnt120419_3312.

    Phylogenomic databases

    eggNOGi COG0189.
    HOGENOMi HOG000265022.
    KOi K01920.
    OMAi DWKIARA.
    OrthoDBi EOG6WMHWB.

    Enzyme and pathway databases

    UniPathwayi UPA00142 ; UER00210 .

    Family and domain databases

    Gene3Di 3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    HAMAPi MF_00162. GSH_S.
    InterProi IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR006284. Glut_synth_pro.
    IPR004218. GSHS_ATP-bd.
    IPR004215. GSHS_N.
    IPR016185. PreATP-grasp_dom.
    [Graphical view ]
    Pfami PF02955. GSH-S_ATP. 1 hit.
    PF02951. GSH-S_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52440. SSF52440. 1 hit.
    TIGRFAMsi TIGR01380. glut_syn. 1 hit.
    PROSITEi PS50975. ATP_GRASP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CT18.
    2. "Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and CT18."
      Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V., Kodoyianni V., Schwartz D.C., Blattner F.R.
      J. Bacteriol. 185:2330-2337(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700931 / Ty2.

    Entry informationi

    Entry nameiGSHB_SALTI
    AccessioniPrimary (citable) accession number: P58581
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2002
    Last sequence update: January 23, 2002
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3