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Protein

Glutathione synthetase

Gene

gshB

Organism
Salmonella typhi
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg2+ or Mn2+ ion per subunit.By similarity

Pathwayi: glutathione biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate--cysteine ligase (gshA)
  2. Glutathione synthetase (gshB)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi281Magnesium or manganeseUniRule annotation1
Metal bindingi283Magnesium or manganeseUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi151 – 207ATPUniRule annotationAdd BLAST57

GO - Molecular functioni

Keywordsi

Molecular functionLigase
Biological processGlutathione biosynthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00142; UER00210

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
Alternative name(s):
GSH synthetaseUniRule annotation
Short name:
GSH-SUniRule annotation
Short name:
GSHaseUniRule annotation
Glutathione synthaseUniRule annotation
Gene namesi
Name:gshBUniRule annotation
Ordered Locus Names:STY3248, t3007
OrganismiSalmonella typhi
Taxonomic identifieri90370 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001974831 – 315Glutathione synthetaseAdd BLAST315

Proteomic databases

PRIDEiP58581

Interactioni

Protein-protein interaction databases

STRINGi220341.STY3248

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LP5model-A1-315[»]
ProteinModelPortaliP58581
SMRiP58581
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini125 – 310ATP-graspUniRule annotationAdd BLAST186

Sequence similaritiesi

Belongs to the prokaryotic GSH synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D7Z Bacteria
COG0189 LUCA
HOGENOMiHOG000265022
KOiK01920
OMAiWMRKDPP

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
HAMAPiMF_00162 GSH_S, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR006284 Glut_synth_pro
IPR004218 GSHS_ATP-bd
IPR004215 GSHS_N
IPR016185 PreATP-grasp_dom_sf
PANTHERiPTHR21621:SF4 PTHR21621:SF4, 1 hit
PfamiView protein in Pfam
PF02955 GSH-S_ATP, 1 hit
PF02951 GSH-S_N, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01380 glut_syn, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit

Sequencei

Sequence statusi: Complete.

P58581-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKLGIVMDP IAHINIKKDT SFAMLLEAQR RGYELHYMEM ADLYLINGEA
60 70 80 90 100
RACTRTLSVE QNYDKWYEFG SEQEIKLADL DVILMRKDPP FDTEFIYATY
110 120 130 140 150
ILERAEEEGT LIVNKPQSLR DCNEKLYTAW FADLTPETLV TRNKAQLKAF
160 170 180 190 200
WEKHGDIIMK PLDGMGGASI FRVKEGDPNI GVIAETLTEL GNRYCMAQNY
210 220 230 240 250
LPAIKDGDKR VLVVDGEPVP YCLARIPQGG ETRGNLAAGG RGEPRPLSES
260 270 280 290 300
DWEIARRVGP TLKAKGLIFV GLDIIGDRLT EINVTSPTCV REIEAEYPIS
310
ITGMLMDAIE ARLAK
Length:315
Mass (Da):35,380
Last modified:January 23, 2002 - v1
Checksum:iD9AC53F9B23070C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL513382 Genomic DNA Translation: CAD02919.1
AE014613 Genomic DNA Translation: AAO70559.1
RefSeqiNP_457487.1, NC_003198.1
WP_000593242.1, NZ_PHKG01000037.1

Genome annotation databases

EnsemblBacteriaiAAO70559; AAO70559; t3007
CAD02919; CAD02919; CAD02919
GeneIDi1249530
KEGGistt:t3007
sty:STY3248
PATRICifig|220341.7.peg.3312

Similar proteinsi

Entry informationi

Entry nameiGSHB_SALTI
AccessioniPrimary (citable) accession number: P58581
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: January 23, 2002
Last modified: March 28, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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