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P58580 (GSHB_SALTY) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione synthetase

EC=6.3.2.3
Alternative name(s):
GSH synthetase
Short name=GSH-S
Short name=GSHase
Glutathione synthase
Gene names
Name:gshB
Ordered Locus Names:STM3095
OrganismSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]
Taxonomic identifier99287 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Protein attributes

Sequence length315 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP-Rule MF_00162

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP-Rule MF_00162

Sequence similarities

Belongs to the prokaryotic GSH synthase family.

Contains 1 ATP-grasp domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 315315Glutathione synthetase HAMAP-Rule MF_00162
PRO_0000197484

Regions

Domain125 – 310186ATP-grasp
Nucleotide binding151 – 20757ATP By similarity

Sites

Metal binding2811Magnesium or manganese By similarity
Metal binding2831Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
P58580 [UniParc].

Last modified January 23, 2002. Version 1.
Checksum: 98AC5A80C230761F

FASTA31535,433
        10         20         30         40         50         60 
MIKLGIVMDP IAHINIKKDT SFAMLLEAQR RGYELHYMEM ADLYLINGEA RARTRTLSVE 

        70         80         90        100        110        120 
QNYDKWYEFG SEQEIKLADL DVILMRKDPP FDTEFIYATY ILERAEEEGT LIVNKPQSLR 

       130        140        150        160        170        180 
DCNEKLYTAW FADLTPETLV TRNKAQLKAF WEKHGDIIMK PLDGMGGASI FRVKEGDPNI 

       190        200        210        220        230        240 
GVIAETLTEL GNRYCMAQNY LPAIKDGDKR VLVVDGEPVP YCLARIPQGG ETRGNLAAGG 

       250        260        270        280        290        300 
RGEPRPLSES DWEIARRVGP TLKAKGLIFV GLDIIGDRLT EINVTSPTCV REIEAEYPIS 

       310 
ITGMLMDAIE ARLAK 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE006468 Genomic DNA. Translation: AAL21970.1.
RefSeqNP_462011.1. NC_003197.1.

3D structure databases

ProteinModelPortalP58580.
SMRP58580. Positions 1-313.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING99287.STM3095.

Proteomic databases

PaxDbP58580.
PRIDEP58580.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL21970; AAL21970; STM3095.
GeneID1254618.
KEGGstm:STM3095.
PATRIC32384943. VBISalEnt20916_3280.

Phylogenomic databases

eggNOGCOG0189.
HOGENOMHOG000265022.
KOK01920.
OMADWKIARA.
OrthoDBEOG6WMHWB.
PhylomeDBP58580.

Enzyme and pathway databases

BioCycSENT99287:GCTI-3115-MONOMER.
UniPathwayUPA00142; UER00210.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00162. GSH_S.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01380. glut_syn. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSHB_SALTY
AccessionPrimary (citable) accession number: P58580
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: January 23, 2002
Last modified: July 9, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways