P58580 (GSHB_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione synthetase
EC=6.3.2.3

Alternative name(s):
GSH synthetase
Short name=GSH-S
Short name=GSHase
Glutathione synthase

Gene names

Name:	gshB
Ordered Locus Names:	STM3095

Organism

Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]

Taxonomic identifier

99287 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella typhimurium

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP-Rule MF_00162
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP-Rule MF_00162
Sequence similarities	Belongs to the prokaryotic GSH synthase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Glutathione biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytosol Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glutathione synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 315	315	Glutathione synthetase HAMAP-Rule MF_00162		PRO_0000197484
Regions
	Domain	125 – 310	186	ATP-grasp
	Nucleotide binding	151 – 207	57	ATP By similarity
Sites
	Metal binding	281	1	Magnesium or manganese By similarity
	Metal binding	283	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	P58580 [UniParc]. Last modified January 23, 2002. Version 1. Checksum: 98AC5A80C230761F	FASTA	315	35,433
	10 20 30 40 50 60 MIKLGIVMDP IAHINIKKDT SFAMLLEAQR RGYELHYMEM ADLYLINGEA RARTRTLSVE 70 80 90 100 110 120 QNYDKWYEFG SEQEIKLADL DVILMRKDPP FDTEFIYATY ILERAEEEGT LIVNKPQSLR 130 140 150 160 170 180 DCNEKLYTAW FADLTPETLV TRNKAQLKAF WEKHGDIIMK PLDGMGGASI FRVKEGDPNI 190 200 210 220 230 240 GVIAETLTEL GNRYCMAQNY LPAIKDGDKR VLVVDGEPVP YCLARIPQGG ETRGNLAAGG 250 260 270 280 290 300 RGEPRPLSES DWEIARRVGP TLKAKGLIFV GLDIIGDRLT EINVTSPTCV REIEAEYPIS 310 ITGMLMDAIE ARLAK

References

[1]

"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006468 Genomic DNA. Translation: AAL21970.1.
RefSeq	NP_462011.1. NC_003197.1.
3D structure databases
ProteinModelPortal	P58580.
SMR	P58580. Positions 1-313.
ModBase	Search...
Protein-protein interaction databases
STRING	99287.STM3095.
Proteomic databases
PaxDb	P58580.
PRIDE	P58580.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL21970; AAL21970; STM3095.
GeneID	1254618.
KEGG	stm:STM3095.
PATRIC	32384943. VBISalEnt20916_3280.
Phylogenomic databases
eggNOG	COG0189.
HOGENOM	HOG000265022.
KO	K01920.
OMA	WMRKDPP.
ProtClustDB	PRK05246.
Enzyme and pathway databases
UniPathway	UPA00142; UER00210.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 1 hit. 3.40.50.20. 1 hit.
HAMAP	MF_00162. GSH-S.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR006284. Glut_synth_pro. IPR004218. GSHS_ATP-bd. IPR004215. GSHS_N. IPR016185. PreATP-grasp_dom. [Graphical view]
Pfam	PF02955. GSH-S_ATP. 1 hit. PF02951. GSH-S_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMs	TIGR01380. glut_syn. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSHB_SALTY

Accession

Primary (citable) accession number: P58580

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	January 23, 2002
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program