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P58579

- GSHB_RALSO

UniProt

P58579 - GSHB_RALSO

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Protein

Glutathione synthetase

Gene
gshB, RSc0345, RS03312
Organism
Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi280 – 2801Magnesium or manganese By similarity
Metal bindingi282 – 2821Magnesium or manganese By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi150 – 20657ATP By similarityAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutathione synthase activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. manganese ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRSOL267608:GCVU-346-MONOMER.
UniPathwayiUPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetase (EC:6.3.2.3)
Alternative name(s):
GSH synthetase
Short name:
GSH-S
Short name:
GSHase
Glutathione synthase
Gene namesi
Name:gshB
Ordered Locus Names:RSc0345
ORF Names:RS03312
OrganismiRalstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Taxonomic identifieri267608 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
ProteomesiUP000001436: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 324324Glutathione synthetaseUniRule annotationPRO_0000197479Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi267608.RSc0345.

Structurei

3D structure databases

ProteinModelPortaliP58579.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini124 – 309186ATP-graspAdd
BLAST

Sequence similaritiesi

Contains 1 ATP-grasp domain.

Phylogenomic databases

eggNOGiCOG0189.
HOGENOMiHOG000265022.
KOiK01920.
OMAiDWKIARA.
OrthoDBiEOG6WMHWB.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_00162. GSH_S.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01380. glut_syn. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P58579-1 [UniParc]FASTAAdd to Basket

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MRILFIVDPL STFKIYKDST FAMMREAAAR GYAIYTCQQS QLTLSGNVVE    50
TVATPLALTG DEHDWYRSGD PRLLPLTGFD AVLMRKDPPF DMEYVTSTWL 100
LEIAERQGAR VFNKPQAIRD HSEKLAIAQF REFTAPTIVT RDAKRLREFH 150
AEQGDVIFKP LDGMGGAGIF RVGADGMNLG SVIETLTHNG TRTVMAQQYI 200
PAIRDGDKRI LLIGGSPVPH ALARVPMAGE VRGNLAAGGT GRAQLLSERD 250
QVIAHALAPV LWQRGLLLVG LDVIGDYLTE VNVTSPTCFQ EITQQTGFNV 300
AGMFIDALER AAGKASGGLG RKLA 324
Length:324
Mass (Da):35,330
Last modified:January 23, 2002 - v1
Checksum:i2C987513B5546758
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL646052 Genomic DNA. Translation: CAD13873.1.
RefSeqiNP_518466.1. NC_003295.1.

Genome annotation databases

EnsemblBacteriaiCAD13873; CAD13873; RSc0345.
GeneIDi1219148.
KEGGirso:RSc0345.
PATRICi20259233. VBIRalSol70888_0351.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL646052 Genomic DNA. Translation: CAD13873.1 .
RefSeqi NP_518466.1. NC_003295.1.

3D structure databases

ProteinModelPortali P58579.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 267608.RSc0345.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD13873 ; CAD13873 ; RSc0345 .
GeneIDi 1219148.
KEGGi rso:RSc0345.
PATRICi 20259233. VBIRalSol70888_0351.

Phylogenomic databases

eggNOGi COG0189.
HOGENOMi HOG000265022.
KOi K01920.
OMAi DWKIARA.
OrthoDBi EOG6WMHWB.

Enzyme and pathway databases

UniPathwayi UPA00142 ; UER00210 .
BioCyci RSOL267608:GCVU-346-MONOMER.

Family and domain databases

Gene3Di 3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPi MF_00162. GSH_S.
InterProi IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view ]
Pfami PF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view ]
SUPFAMi SSF52440. SSF52440. 1 hit.
TIGRFAMsi TIGR01380. glut_syn. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GMI1000.

Entry informationi

Entry nameiGSHB_RALSO
AccessioniPrimary (citable) accession number: P58579
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: January 23, 2002
Last modified: May 14, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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