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Protein

Glutathione synthetase

Gene

gshB

Organism
Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg(2+) or Mn2+ ion per subunit.By similarity

Pathway: glutathione biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glutathione synthetase (gshB)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi280 – 2801Magnesium or manganeseUniRule annotation
Metal bindingi282 – 2821Magnesium or manganeseUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi150 – 20657ATPUniRule annotationAdd
BLAST

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRSOL267608:GCVU-346-MONOMER.
UniPathwayiUPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
Alternative name(s):
GSH synthetaseUniRule annotation
Short name:
GSH-SUniRule annotation
Short name:
GSHaseUniRule annotation
Glutathione synthaseUniRule annotation
Gene namesi
Name:gshBUniRule annotation
Ordered Locus Names:RSc0345
ORF Names:RS03312
OrganismiRalstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Taxonomic identifieri267608 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
ProteomesiUP000001436 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 324324Glutathione synthetasePRO_0000197479Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi267608.RSc0345.

Structurei

3D structure databases

ProteinModelPortaliP58579.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini124 – 309186ATP-graspUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic GSH synthase family.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0189.
HOGENOMiHOG000265022.
KOiK01920.
OMAiMKPLHGH.
OrthoDBiEOG6WMHWB.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_00162. GSH_S.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01380. glut_syn. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P58579-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRILFIVDPL STFKIYKDST FAMMREAAAR GYAIYTCQQS QLTLSGNVVE
60 70 80 90 100
TVATPLALTG DEHDWYRSGD PRLLPLTGFD AVLMRKDPPF DMEYVTSTWL
110 120 130 140 150
LEIAERQGAR VFNKPQAIRD HSEKLAIAQF REFTAPTIVT RDAKRLREFH
160 170 180 190 200
AEQGDVIFKP LDGMGGAGIF RVGADGMNLG SVIETLTHNG TRTVMAQQYI
210 220 230 240 250
PAIRDGDKRI LLIGGSPVPH ALARVPMAGE VRGNLAAGGT GRAQLLSERD
260 270 280 290 300
QVIAHALAPV LWQRGLLLVG LDVIGDYLTE VNVTSPTCFQ EITQQTGFNV
310 320
AGMFIDALER AAGKASGGLG RKLA
Length:324
Mass (Da):35,330
Last modified:January 23, 2002 - v1
Checksum:i2C987513B5546758
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL646052 Genomic DNA. Translation: CAD13873.1.
RefSeqiNP_518466.1. NC_003295.1.
WP_011000309.1. NC_003295.1.

Genome annotation databases

EnsemblBacteriaiCAD13873; CAD13873; RSc0345.
GeneIDi1219148.
KEGGirso:RSc0345.
PATRICi20259233. VBIRalSol70888_0351.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL646052 Genomic DNA. Translation: CAD13873.1.
RefSeqiNP_518466.1. NC_003295.1.
WP_011000309.1. NC_003295.1.

3D structure databases

ProteinModelPortaliP58579.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi267608.RSc0345.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAD13873; CAD13873; RSc0345.
GeneIDi1219148.
KEGGirso:RSc0345.
PATRICi20259233. VBIRalSol70888_0351.

Phylogenomic databases

eggNOGiCOG0189.
HOGENOMiHOG000265022.
KOiK01920.
OMAiMKPLHGH.
OrthoDBiEOG6WMHWB.

Enzyme and pathway databases

UniPathwayiUPA00142; UER00210.
BioCyciRSOL267608:GCVU-346-MONOMER.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_00162. GSH_S.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01380. glut_syn. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GMI1000.

Entry informationi

Entry nameiGSHB_RALSO
AccessioniPrimary (citable) accession number: P58579
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: January 23, 2002
Last modified: May 27, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.