P58546 (MTPN_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Myotrophin

Alternative name(s):
Protein V-1

Gene names

Name:

MTPN

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	118 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Promotes dimerization of NF-kappa-B subunits and regulates NF-kappa-B transcription factor activity By similarity. Plays a role in the regulation of the growth of actin filaments. Inhibits the activity of the F-actin-capping protein complex formed by the CAPZA1 and CAPZB heterodimer. Promotes growth of cardiomyocytes, but not cardiomyocyte proliferation. Promotes cardiac muscle hypertrophy. Ref.1 Ref.4 Ref.7
Subunit structure	Interacts with RELA By similarity. Interacts with the heterodimer formed by CAPZA1 and CAPZB. Ref.4
Subcellular location	Cytoplasm Probable. Nucleus By similarity. Cytoplasm › perinuclear region By similarity.
Tissue specificity	Ubiquitous. Ref.1
Induction	Up-regulated in heart left ventricle of patients with severe coronary artery disease and history of myocardial ischemia. Up-regulated in heart left ventricle of patients with dilated cardiomyopathy. Ref.1
Miscellaneous	This protein is produced by a bicistronic gene which also produces the MPD6 protein from a non-overlapping reading frame. MPD6 belongs to a group of cryptic antigens without conventional genomic structure. It is encoded by a cryptic open reading frame located in the 3'-untranslated region of MTPN.
Sequence similarities	Belongs to the myotrophin family. Contains 3 ANK repeats.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Domain	ANK repeat Repeat
PTM	Acetylation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	catecholamine metabolic process Inferred from electronic annotation. Source: Compara cell growth Inferred from direct assay Ref.1. Source: UniProtKB cerebellar granule cell differentiation Inferred from electronic annotation. Source: Compara neuron differentiation Non-traceable author statement PubMed 12031792. Source: UniProtKB positive regulation of NF-kappaB transcription factor activity Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of cardiac muscle hypertrophy Inferred from mutant phenotype Ref.1. Source: UniProtKB positive regulation of cell growth Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of macromolecule biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of protein metabolic process Inferred from sequence or structural similarity. Source: UniProtKB regulation of barbed-end actin filament capping Inferred from direct assay Ref.4. Source: UniProtKB regulation of striated muscle tissue development Non-traceable author statement PubMed 12031792. Source: UniProtKB regulation of translation Non-traceable author statement PubMed 12031792. Source: UniProtKB skeletal muscle tissue regeneration Inferred from electronic annotation. Source: Compara striated muscle cell differentiation Inferred from electronic annotation. Source: Compara
Cellular_component	F-actin capping protein complex Inferred from direct assay Ref.4. Source: UniProtKB axon Inferred from electronic annotation. Source: Compara cytosol Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.5

Chain

2 – 118

117

Myotrophin

PRO_0000067031

Regions

Repeat

2 – 30

29

ANK 1

Repeat

34 – 66

33

ANK 2

Repeat

67 – 99

33

ANK 3

Amino acid modifications

Modified residue

2

1

N-acetylcysteine Ref.5

Modified residue

4

1

N6-acetyllysine Ref.5

Modified residue

11

1

N6-acetyllysine Ref.5

Modified residue

24

1

N6-acetyllysine Ref.5

Secondary structure

1

.

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118

Helix Strand Turn

Details...

Helix

3 – 11

9

Helix

15 – 23

9

Helix

38 – 44

7

Helix

48 – 55

8

Turn

56 – 58

3

Helix

71 – 78

8

Helix

81 – 89

9

Helix

104 – 107

4

Helix

111 – 117

7

Sequences

	Sequence		Length	Mass (Da)
	P58546 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 9097FFDF61D329A2	FASTA	118	12,895
	10 20 30 40 50 60 MCDKEFMWAL KNGDLDEVKD YVAKGEDVNR TLEGGRKPLH YAADCGQLEI LEFLLLKGAD 70 80 90 100 110 INAPDKHHIT PLLSAVYEGH VSCVKLLLSK GADKTVKGPD GLTAFEATDN QAIKALLQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA sequence and characterization of the gene that encodes human myotrophin/V-1 protein, a mediator of cardiac hypertrophy." Anderson K.M., Berrebi-Bertrand I., Kirkpatrick R.B., McQueney M.S., Underwood D.C., Rouanet S., Chabot-Fletcher M. J. Mol. Cell. Cardiol. 31:705-719(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, TISSUE SPECIFICITY.
[2]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[4]	"Binding of myotrophin/V-1 to actin-capping protein: implications for how capping protein binds to the filament barbed end." Bhattacharya N., Ghosh S., Sept D., Cooper J.A. J. Biol. Chem. 281:31021-31030(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN AN ACTIN CAPPING COMPLEX, INTERACTION WITH WITH CAPZA1 AND CAPZB.
[5]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT CYS-2; LYS-4; LYS-11 AND LYS-24, MASS SPECTROMETRY, CLEAVAGE OF INITIATOR METHIONINE.
[6]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]	"Two distinct mechanisms for actin capping protein regulation--steric and allosteric inhibition." Takeda S., Minakata S., Koike R., Kawahata I., Narita A., Kitazawa M., Ota M., Yamakuni T., Maeda Y., Nitanai Y. PLoS Biol. 8:E1000416-E1000416(2010) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) IN COMPLEX WITH CAPZA1 AND CAPZB, FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AC015987 Genomic DNA. No translation available.
BC028093 mRNA. Translation: AAH28093.1.

IPI

IPI00924816.

RefSeq

NP_665807.1. NM_145808.3.

UniGene

Hs.602015.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3AAA	X-ray	2.20	C	1-118	[»]

ProteinModelPortal

P58546.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-50234N.

IntAct

P58546. 3 interactions.

PTM databases

PhosphoSite

P58546.

Polymorphism databases

DMDM

20138912.

2D gel databases

OGP

P58546.

Proteomic databases

PaxDb

P58546.

PeptideAtlas

P58546.

PRIDE

P58546.

Protocols and materials databases

DNASU

136319.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000393085; ENSP00000376800; ENSG00000105887.

GeneID

136319.

KEGG

hsa:136319.

UCSC

uc003vte.4. human.

Organism-specific databases

CTD

136319.

GeneCards

GC07M135613.

HGNC

HGNC:15667. MTPN.

HPA

HPA019735.

MIM

606484. gene.

neXtProt

NX_P58546.

PharmGKB

PA31271.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG0666.

HOGENOM

HOG000276399.

HOVERGEN

HBG019067.

InParanoid

P58546.

OMA

MSDKEFM.

OrthoDB

EOG470TJP.

PhylomeDB

P58546.

Gene expression databases

ArrayExpress

P58546.

Bgee

P58546.

CleanEx

HS_MTPN.

Genevestigator

P58546.

Family and domain databases

Gene3D

1.25.40.20. 1 hit.

InterPro

IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]

Pfam

PF12796. Ank_2. 1 hit.
[Graphical view]

PRINTS

PR01415. ANKYRIN.

SMART

SM00248. ANK. 2 hits.
[Graphical view]

SUPFAM

SSF48403. ANK. 1 hit.

PROSITE

PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

MTPN. human.

EvolutionaryTrace

P58546.

GenomeRNAi

136319.

NextBio

83582.

SOURCE

Search...

Entry information

Entry name

MTPN_HUMAN

Accession

Primary (citable) accession number: P58546

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 107 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.