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P58502 (TKSU_THEKO) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein attributes

Sequence length422 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has a broad substrate specificity with a slight preference to large hydrophobic amino acid residues at the P1 position.

Cofactor

Calcium.

Subunit structure

Monomer.

Subcellular location

Secreted.

Sequence similarities

Belongs to the peptidase S8 family.

Biophysicochemical properties

pH dependence:

Optimum pH is 9.5.

Temperature dependence:

Thermostable. Highly active at 80 degrees Celsius.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   LigandCalcium
   Molecular functionHydrolase
Protease
Serine protease
   PTMZymogen
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionidentical protein binding

Inferred from physical interaction PubMed 22483987. Source: IntAct

serine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself2EBI-7810114,EBI-7810114

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Propeptide25 – 10682 Potential
PRO_0000027195
Chain107 – 422316Tk-subtilisin
PRO_0000027196

Sites

Active site1391Charge relay system By similarity
Active site1771Charge relay system By similarity
Active site3481Charge relay system By similarity

Secondary structure

.............................................................................. 422
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P58502 [UniParc].

Last modified December 19, 2001. Version 1.
Checksum: 843255BCD806DB71

FASTA42243,786
        10         20         30         40         50         60 
MKKSIALVLS IVLLAALFAV PASAGEQNTI RVIVSVDKAK FNPHEVLGIG GHIVYQFKLI 

        70         80         90        100        110        120 
PAVVVDVPAN AVGKLKKMPG VEKVEFDHQA VLLGKPSWLG GGSTQPAQTI PWGIERVKAP 

       130        140        150        160        170        180 
SVWSITDGSV SVIQVAVLDT GVDYDHPDLA ANIAWCVSTL RGKVSTKLRD CADQNGHGTH 

       190        200        210        220        230        240 
VIGTIAALNN DIGVVGVAPG VQIYSVRVLD ARGSGSYSDI AIGIEQAILG PDGVADKDGD 

       250        260        270        280        290        300 
GIIAGDPDDD AAEVISMSLG GPADDSYLYD MIIQAYNAGI VIVAASGNEG APSPSYPAAY 

       310        320        330        340        350        360 
PEVIAVGAID SNDNIASFSN RQPEVSAPGV DILSTYPDDS YETLMGTSMA TPHVSGVVAL 

       370        380        390        400        410        420 
IQAAYYQKYG KILPVGTFDD ISKNTVRGIL HITADDLGPT GWDADYGYGV VRAALAVQAA 


LG 

« Hide

References

« Hide 'large scale' references
[1]"Active subtilisin-like protease from a hyperthermophilic archaeon in a form with a putative prosequence."
Kannan Y., Koga Y., Inoue Y., Haruki M., Takagi M., Imanaka T., Morikawa M., Kanaya S.
Appl. Environ. Microbiol. 67:2445-2452(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
Strain: ATCC BAA-918 / JCM 12380 / KOD1.
[2]"Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes."
Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.
Genome Res. 15:352-363(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-918 / JCM 12380 / KOD1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB056701 Genomic DNA. Translation: BAB60701.1.
AP006878 Genomic DNA. Translation: BAD85864.1.
RefSeqYP_184088.1. NC_006624.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2E1PX-ray2.30A25-422[»]
2Z2XX-ray1.70A105-422[»]
2Z2YX-ray1.89A/C105-422[»]
B/D29-93[»]
2Z2ZX-ray1.87A28-422[»]
2Z30X-ray1.65A103-422[»]
B29-93[»]
2Z56X-ray1.90A105-422[»]
B29-93[»]
2Z57X-ray1.80A105-422[»]
B29-93[»]
2Z58X-ray1.88A105-422[»]
B28-93[»]
2ZRQX-ray2.16A94-422[»]
2ZWOX-ray2.07A/B/C25-422[»]
2ZWPX-ray2.40A/B25-422[»]
3A3NX-ray2.20A94-422[»]
B25-91[»]
3A3OX-ray1.90A94-422[»]
B25-88[»]
3A3PX-ray1.90A94-422[»]
B25-93[»]
3VHQX-ray2.15A25-422[»]
3VV2X-ray1.83A94-422[»]
B25-93[»]
4JP8X-ray2.21A25-422[»]
ProteinModelPortalP58502.
SMRP58502. Positions 28-422.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-8404006.
STRING69014.TK1675.

Protein family/group databases

MEROPSS08.129.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD85864; BAD85864; TK1675.
GeneID3234572.
KEGGtko:TK1675.

Phylogenomic databases

eggNOGCOG1404.
HOGENOMHOG000199176.
KOK01362.

Enzyme and pathway databases

BioCycTKOD69014:GH72-1707-MONOMER.

Family and domain databases

Gene3D3.40.50.200. 1 hit.
InterProIPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR009020. Prot_inh_propept.
[Graphical view]
PANTHERPTHR10795. PTHR10795. 1 hit.
PfamPF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSPR00723. SUBTILISIN.
SUPFAMSSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEPS00136. SUBTILASE_ASP. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP58502.

Entry information

Entry nameTKSU_THEKO
AccessionPrimary (citable) accession number: P58502
Secondary accession number(s): Q977F5
Entry history
Integrated into UniProtKB/Swiss-Prot: December 19, 2001
Last sequence update: December 19, 2001
Last modified: July 9, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references