Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Tk-subtilisin

Gene

TK1675

Organism
Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has a broad substrate specificity with a slight preference to large hydrophobic amino acid residues at the P1 position.

Cofactori

pH dependencei

Optimum pH is 9.5.

Temperature dependencei

Thermostable. Highly active at 80 degrees Celsius.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei139Charge relay systemBy similarity1
Active sitei177Charge relay systemBy similarity1
Active sitei348Charge relay systemBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Ligandi

Calcium

Enzyme and pathway databases

BioCyciTKOD69014:GH72-1708-MONOMER.
BRENDAi3.4.21.B57. 5246.

Protein family/group databases

MEROPSiS08.129.

Names & Taxonomyi

Protein namesi
Recommended name:
Tk-subtilisin (EC:3.4.21.-)
Gene namesi
Ordered Locus Names:TK1675
OrganismiThermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Taxonomic identifieri69014 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
Proteomesi
  • UP000000536 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
PropeptideiPRO_000002719525 – 106Sequence analysisAdd BLAST82
ChainiPRO_0000027196107 – 422Tk-subtilisinAdd BLAST316

Keywords - PTMi

Zymogen

Interactioni

Subunit structurei

Monomer.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-7810114,EBI-7810114

Protein-protein interaction databases

MINTiMINT-8404006.
STRINGi69014.TK1675.

Structurei

Secondary structure

1422
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi30 – 36Combined sources7
Helixi38 – 40Combined sources3
Helixi43 – 49Combined sources7
Beta strandi52 – 56Combined sources5
Beta strandi58 – 67Combined sources10
Helixi69 – 71Combined sources3
Helixi72 – 76Combined sources5
Beta strandi81 – 86Combined sources6
Beta strandi89 – 92Combined sources4
Beta strandi95 – 98Combined sources4
Beta strandi105 – 107Combined sources3
Helixi112 – 116Combined sources5
Helixi120 – 122Combined sources3
Turni123 – 125Combined sources3
Beta strandi134 – 140Combined sources7
Turni147 – 149Combined sources3
Helixi150 – 152Combined sources3
Beta strandi153 – 158Combined sources6
Helixi160 – 162Combined sources3
Helixi168 – 171Combined sources4
Beta strandi174 – 176Combined sources3
Helixi177 – 186Combined sources10
Beta strandi189 – 193Combined sources5
Beta strandi202 – 207Combined sources6
Beta strandi213 – 216Combined sources4
Helixi217 – 229Combined sources13
Turni230 – 233Combined sources4
Beta strandi239 – 241Combined sources3
Beta strandi253 – 257Combined sources5
Beta strandi259 – 262Combined sources4
Helixi266 – 277Combined sources12
Beta strandi281 – 285Combined sources5
Turni297 – 299Combined sources3
Beta strandi303 – 309Combined sources7
Beta strandi324 – 328Combined sources5
Beta strandi330 – 336Combined sources7
Turni337 – 339Combined sources3
Beta strandi340 – 344Combined sources5
Helixi347 – 369Combined sources23
Beta strandi382 – 385Combined sources4
Helixi386 – 393Combined sources8
Beta strandi398 – 403Combined sources6
Turni404 – 406Combined sources3
Helixi413 – 421Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2E1PX-ray2.30A25-422[»]
2Z2XX-ray1.70A105-422[»]
2Z2YX-ray1.89A/C105-422[»]
B/D29-93[»]
2Z2ZX-ray1.87A28-422[»]
2Z30X-ray1.65A103-422[»]
B29-93[»]
2Z56X-ray1.90A105-422[»]
B29-93[»]
2Z57X-ray1.80A105-422[»]
B29-93[»]
2Z58X-ray1.88A105-422[»]
B28-93[»]
2ZRQX-ray2.16A94-422[»]
2ZWOX-ray2.07A/B/C25-422[»]
2ZWPX-ray2.40A/B25-422[»]
3A3NX-ray2.20A94-422[»]
B25-91[»]
3A3OX-ray1.90A94-422[»]
B25-88[»]
3A3PX-ray1.90A94-422[»]
B25-93[»]
3VHQX-ray2.15A25-422[»]
3VV2X-ray1.83A94-422[»]
B25-93[»]
3WIUX-ray1.80A/B/C25-422[»]
3WIVX-ray1.90A/B/C25-422[»]
4JP8X-ray2.21A25-422[»]
ProteinModelPortaliP58502.
SMRiP58502.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP58502.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini135 – 417Peptidase S8Add BLAST283

Sequence similaritiesi

Belongs to the peptidase S8 family.Curated
Contains 1 peptidase S8 domain.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiarCOG00702. Archaea.
COG1404. LUCA.
HOGENOMiHOG000199176.
InParanoidiP58502.
OMAiDACDEAY.

Family and domain databases

Gene3Di3.30.70.80. 1 hit.
3.40.50.200. 1 hit.
InterProiIPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR009020. Propept_inh.
IPR010259. S8pro/Inhibitor_I9.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 2 hits.
PfamiPF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P58502-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKSIALVLS IVLLAALFAV PASAGEQNTI RVIVSVDKAK FNPHEVLGIG
60 70 80 90 100
GHIVYQFKLI PAVVVDVPAN AVGKLKKMPG VEKVEFDHQA VLLGKPSWLG
110 120 130 140 150
GGSTQPAQTI PWGIERVKAP SVWSITDGSV SVIQVAVLDT GVDYDHPDLA
160 170 180 190 200
ANIAWCVSTL RGKVSTKLRD CADQNGHGTH VIGTIAALNN DIGVVGVAPG
210 220 230 240 250
VQIYSVRVLD ARGSGSYSDI AIGIEQAILG PDGVADKDGD GIIAGDPDDD
260 270 280 290 300
AAEVISMSLG GPADDSYLYD MIIQAYNAGI VIVAASGNEG APSPSYPAAY
310 320 330 340 350
PEVIAVGAID SNDNIASFSN RQPEVSAPGV DILSTYPDDS YETLMGTSMA
360 370 380 390 400
TPHVSGVVAL IQAAYYQKYG KILPVGTFDD ISKNTVRGIL HITADDLGPT
410 420
GWDADYGYGV VRAALAVQAA LG
Length:422
Mass (Da):43,786
Last modified:December 19, 2001 - v1
Checksum:i843255BCD806DB71
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056701 Genomic DNA. Translation: BAB60701.1.
AP006878 Genomic DNA. Translation: BAD85864.1.
RefSeqiWP_011250626.1. NC_006624.1.

Genome annotation databases

EnsemblBacteriaiBAD85864; BAD85864; TK1675.
GeneIDi3234572.
KEGGitko:TK1675.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056701 Genomic DNA. Translation: BAB60701.1.
AP006878 Genomic DNA. Translation: BAD85864.1.
RefSeqiWP_011250626.1. NC_006624.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2E1PX-ray2.30A25-422[»]
2Z2XX-ray1.70A105-422[»]
2Z2YX-ray1.89A/C105-422[»]
B/D29-93[»]
2Z2ZX-ray1.87A28-422[»]
2Z30X-ray1.65A103-422[»]
B29-93[»]
2Z56X-ray1.90A105-422[»]
B29-93[»]
2Z57X-ray1.80A105-422[»]
B29-93[»]
2Z58X-ray1.88A105-422[»]
B28-93[»]
2ZRQX-ray2.16A94-422[»]
2ZWOX-ray2.07A/B/C25-422[»]
2ZWPX-ray2.40A/B25-422[»]
3A3NX-ray2.20A94-422[»]
B25-91[»]
3A3OX-ray1.90A94-422[»]
B25-88[»]
3A3PX-ray1.90A94-422[»]
B25-93[»]
3VHQX-ray2.15A25-422[»]
3VV2X-ray1.83A94-422[»]
B25-93[»]
3WIUX-ray1.80A/B/C25-422[»]
3WIVX-ray1.90A/B/C25-422[»]
4JP8X-ray2.21A25-422[»]
ProteinModelPortaliP58502.
SMRiP58502.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-8404006.
STRINGi69014.TK1675.

Protein family/group databases

MEROPSiS08.129.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD85864; BAD85864; TK1675.
GeneIDi3234572.
KEGGitko:TK1675.

Phylogenomic databases

eggNOGiarCOG00702. Archaea.
COG1404. LUCA.
HOGENOMiHOG000199176.
InParanoidiP58502.
OMAiDACDEAY.

Enzyme and pathway databases

BioCyciTKOD69014:GH72-1708-MONOMER.
BRENDAi3.4.21.B57. 5246.

Miscellaneous databases

EvolutionaryTraceiP58502.

Family and domain databases

Gene3Di3.30.70.80. 1 hit.
3.40.50.200. 1 hit.
InterProiIPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR009020. Propept_inh.
IPR010259. S8pro/Inhibitor_I9.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 2 hits.
PfamiPF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTKSU_THEKO
AccessioniPrimary (citable) accession number: P58502
Secondary accession number(s): Q977F5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 19, 2001
Last sequence update: December 19, 2001
Last modified: November 2, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.