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P58502

- TKSU_THEKO

UniProt

P58502 - TKSU_THEKO

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Protein
Tk-subtilisin
Gene
TK1675
Organism
Thermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Has a broad substrate specificity with a slight preference to large hydrophobic amino acid residues at the P1 position.

Cofactori

Calcium.

pH dependencei

Optimum pH is 9.5.

Temperature dependencei

Thermostable. Highly active at 80 degrees Celsius.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei139 – 1391Charge relay system By similarity
Active sitei177 – 1771Charge relay system By similarity
Active sitei348 – 3481Charge relay system By similarity

GO - Molecular functioni

  1. identical protein binding Source: IntAct
  2. serine-type endopeptidase activity Source: InterPro
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Ligandi

    Calcium

    Enzyme and pathway databases

    BioCyciTKOD69014:GH72-1707-MONOMER.

    Protein family/group databases

    MEROPSiS08.129.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tk-subtilisin (EC:3.4.21.-)
    Gene namesi
    Ordered Locus Names:TK1675
    OrganismiThermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
    Taxonomic identifieri69014 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
    ProteomesiUP000000536: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424 Reviewed prediction
    Add
    BLAST
    Propeptidei25 – 10682 Reviewed prediction
    PRO_0000027195Add
    BLAST
    Chaini107 – 422316Tk-subtilisin
    PRO_0000027196Add
    BLAST

    Keywords - PTMi

    Zymogen

    Interactioni

    Subunit structurei

    Monomer.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself2EBI-7810114,EBI-7810114

    Protein-protein interaction databases

    MINTiMINT-8404006.
    STRINGi69014.TK1675.

    Structurei

    Secondary structure

    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi30 – 367
    Helixi38 – 403
    Helixi43 – 497
    Beta strandi52 – 565
    Beta strandi58 – 6710
    Helixi69 – 713
    Helixi72 – 765
    Beta strandi81 – 866
    Beta strandi89 – 924
    Beta strandi95 – 984
    Beta strandi105 – 1073
    Helixi112 – 1165
    Helixi120 – 1223
    Turni123 – 1253
    Beta strandi134 – 1407
    Turni147 – 1493
    Helixi150 – 1523
    Beta strandi153 – 1586
    Helixi160 – 1623
    Helixi168 – 1714
    Beta strandi174 – 1763
    Helixi177 – 18610
    Beta strandi189 – 1935
    Beta strandi202 – 2076
    Beta strandi213 – 2164
    Helixi217 – 22913
    Turni230 – 2334
    Beta strandi239 – 2413
    Beta strandi253 – 2575
    Beta strandi259 – 2624
    Helixi266 – 27712
    Beta strandi281 – 2855
    Turni297 – 2993
    Beta strandi303 – 3097
    Beta strandi324 – 3285
    Beta strandi330 – 3367
    Turni337 – 3393
    Beta strandi340 – 3445
    Helixi347 – 36923
    Beta strandi382 – 3854
    Helixi386 – 3938
    Beta strandi398 – 4036
    Turni404 – 4063
    Helixi413 – 4219

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2E1PX-ray2.30A25-422[»]
    2Z2XX-ray1.70A105-422[»]
    2Z2YX-ray1.89A/C105-422[»]
    B/D29-93[»]
    2Z2ZX-ray1.87A28-422[»]
    2Z30X-ray1.65A103-422[»]
    B29-93[»]
    2Z56X-ray1.90A105-422[»]
    B29-93[»]
    2Z57X-ray1.80A105-422[»]
    B29-93[»]
    2Z58X-ray1.88A105-422[»]
    B28-93[»]
    2ZRQX-ray2.16A94-422[»]
    2ZWOX-ray2.07A/B/C25-422[»]
    2ZWPX-ray2.40A/B25-422[»]
    3A3NX-ray2.20A94-422[»]
    B25-91[»]
    3A3OX-ray1.90A94-422[»]
    B25-88[»]
    3A3PX-ray1.90A94-422[»]
    B25-93[»]
    3VHQX-ray2.15A25-422[»]
    3VV2X-ray1.83A94-422[»]
    B25-93[»]
    4JP8X-ray2.21A25-422[»]
    ProteinModelPortaliP58502.
    SMRiP58502. Positions 28-422.

    Miscellaneous databases

    EvolutionaryTraceiP58502.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase S8 family.

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1404.
    HOGENOMiHOG000199176.
    KOiK01362.

    Family and domain databases

    Gene3Di3.40.50.200. 1 hit.
    InterProiIPR000209. Peptidase_S8/S53_dom.
    IPR023827. Peptidase_S8_Asp-AS.
    IPR023828. Peptidase_S8_Ser-AS.
    IPR015500. Peptidase_S8_subtilisin-rel.
    IPR009020. Prot_inh_propept.
    [Graphical view]
    PANTHERiPTHR10795. PTHR10795. 1 hit.
    PfamiPF00082. Peptidase_S8. 1 hit.
    [Graphical view]
    PRINTSiPR00723. SUBTILISIN.
    SUPFAMiSSF52743. SSF52743. 1 hit.
    SSF54897. SSF54897. 1 hit.
    PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
    PS00138. SUBTILASE_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P58502-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKSIALVLS IVLLAALFAV PASAGEQNTI RVIVSVDKAK FNPHEVLGIG    50
    GHIVYQFKLI PAVVVDVPAN AVGKLKKMPG VEKVEFDHQA VLLGKPSWLG 100
    GGSTQPAQTI PWGIERVKAP SVWSITDGSV SVIQVAVLDT GVDYDHPDLA 150
    ANIAWCVSTL RGKVSTKLRD CADQNGHGTH VIGTIAALNN DIGVVGVAPG 200
    VQIYSVRVLD ARGSGSYSDI AIGIEQAILG PDGVADKDGD GIIAGDPDDD 250
    AAEVISMSLG GPADDSYLYD MIIQAYNAGI VIVAASGNEG APSPSYPAAY 300
    PEVIAVGAID SNDNIASFSN RQPEVSAPGV DILSTYPDDS YETLMGTSMA 350
    TPHVSGVVAL IQAAYYQKYG KILPVGTFDD ISKNTVRGIL HITADDLGPT 400
    GWDADYGYGV VRAALAVQAA LG 422
    Length:422
    Mass (Da):43,786
    Last modified:December 19, 2001 - v1
    Checksum:i843255BCD806DB71
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB056701 Genomic DNA. Translation: BAB60701.1.
    AP006878 Genomic DNA. Translation: BAD85864.1.
    RefSeqiWP_011250626.1. NC_006624.1.
    YP_184088.1. NC_006624.1.

    Genome annotation databases

    EnsemblBacteriaiBAD85864; BAD85864; TK1675.
    GeneIDi3234572.
    KEGGitko:TK1675.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB056701 Genomic DNA. Translation: BAB60701.1 .
    AP006878 Genomic DNA. Translation: BAD85864.1 .
    RefSeqi WP_011250626.1. NC_006624.1.
    YP_184088.1. NC_006624.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2E1P X-ray 2.30 A 25-422 [» ]
    2Z2X X-ray 1.70 A 105-422 [» ]
    2Z2Y X-ray 1.89 A/C 105-422 [» ]
    B/D 29-93 [» ]
    2Z2Z X-ray 1.87 A 28-422 [» ]
    2Z30 X-ray 1.65 A 103-422 [» ]
    B 29-93 [» ]
    2Z56 X-ray 1.90 A 105-422 [» ]
    B 29-93 [» ]
    2Z57 X-ray 1.80 A 105-422 [» ]
    B 29-93 [» ]
    2Z58 X-ray 1.88 A 105-422 [» ]
    B 28-93 [» ]
    2ZRQ X-ray 2.16 A 94-422 [» ]
    2ZWO X-ray 2.07 A/B/C 25-422 [» ]
    2ZWP X-ray 2.40 A/B 25-422 [» ]
    3A3N X-ray 2.20 A 94-422 [» ]
    B 25-91 [» ]
    3A3O X-ray 1.90 A 94-422 [» ]
    B 25-88 [» ]
    3A3P X-ray 1.90 A 94-422 [» ]
    B 25-93 [» ]
    3VHQ X-ray 2.15 A 25-422 [» ]
    3VV2 X-ray 1.83 A 94-422 [» ]
    B 25-93 [» ]
    4JP8 X-ray 2.21 A 25-422 [» ]
    ProteinModelPortali P58502.
    SMRi P58502. Positions 28-422.
    ModBasei Search...

    Protein-protein interaction databases

    MINTi MINT-8404006.
    STRINGi 69014.TK1675.

    Protein family/group databases

    MEROPSi S08.129.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAD85864 ; BAD85864 ; TK1675 .
    GeneIDi 3234572.
    KEGGi tko:TK1675.

    Phylogenomic databases

    eggNOGi COG1404.
    HOGENOMi HOG000199176.
    KOi K01362.

    Enzyme and pathway databases

    BioCyci TKOD69014:GH72-1707-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P58502.

    Family and domain databases

    Gene3Di 3.40.50.200. 1 hit.
    InterProi IPR000209. Peptidase_S8/S53_dom.
    IPR023827. Peptidase_S8_Asp-AS.
    IPR023828. Peptidase_S8_Ser-AS.
    IPR015500. Peptidase_S8_subtilisin-rel.
    IPR009020. Prot_inh_propept.
    [Graphical view ]
    PANTHERi PTHR10795. PTHR10795. 1 hit.
    Pfami PF00082. Peptidase_S8. 1 hit.
    [Graphical view ]
    PRINTSi PR00723. SUBTILISIN.
    SUPFAMi SSF52743. SSF52743. 1 hit.
    SSF54897. SSF54897. 1 hit.
    PROSITEi PS00136. SUBTILASE_ASP. 1 hit.
    PS00138. SUBTILASE_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    « Hide 'large scale' publications
    1. "Active subtilisin-like protease from a hyperthermophilic archaeon in a form with a putative prosequence."
      Kannan Y., Koga Y., Inoue Y., Haruki M., Takagi M., Imanaka T., Morikawa M., Kanaya S.
      Appl. Environ. Microbiol. 67:2445-2452(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
      Strain: ATCC BAA-918 / JCM 12380 / KOD1.
    2. "Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes."
      Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.
      Genome Res. 15:352-363(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-918 / JCM 12380 / KOD1.

    Entry informationi

    Entry nameiTKSU_THEKO
    AccessioniPrimary (citable) accession number: P58502
    Secondary accession number(s): Q977F5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 19, 2001
    Last sequence update: December 19, 2001
    Last modified: September 3, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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