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P58498

- KAX64_PANIM

UniProt

P58498 - KAX64_PANIM

Protein

Potassium channel toxin alpha-KTx 6.4

Gene
N/A
Organism
Pandinus imperator (Emperor scorpion)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Potently, completely and reversibly blocks voltage-gated potassium channel Kv1.2/KCNA2 and Shaker B (Sh). Also blocks small conductance (SK) calcium-activated potassium channel (KCNN).1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei10 – 101Important for the interaction with Kv1.2/KCNA2 channel
    Sitei19 – 191Important for the interaction with Kv1.2/KCNA2 channel
    Sitei26 – 261Basic residue of the functional dyad, important for the interaction with Kv1.2/KCNA2 channel
    Sitei28 – 281Important for the interaction with Kv1.2/KCNA2 channel
    Sitei30 – 301Important for the interaction with Kv1.2/KCNA2 channel
    Sitei33 – 331Important for the interaction with Kv1.2/KCNA2 channel
    Sitei35 – 351Aromatic residue of the functional dyad, important for the interaction with Kv1.2/KCNA2 channel

    GO - Molecular functioni

    1. ion channel inhibitor activity Source: InterPro

    GO - Biological processi

    1. defense response Source: InterPro
    2. pathogenesis Source: InterPro

    Keywords - Molecular functioni

    Ion channel impairing toxin, Potassium channel impairing toxin, Toxin, Voltage-gated potassium channel impairing toxin

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Potassium channel toxin alpha-KTx 6.4
    Alternative name(s):
    Potassium channel-blocking toxin 4
    Short name:
    Pi-4
    Short name:
    Pi4
    OrganismiPandinus imperator (Emperor scorpion)
    Taxonomic identifieri55084 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesIuridaScorpionoideaScorpionidaeScorpioninaePandinus

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Toxic dosei

    LD50 is 10 µg/kg by intracerebroventricular injection into mice.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Peptidei1 – 3838Potassium channel toxin alpha-KTx 6.4PRO_0000044913Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi6 ↔ 27
    Disulfide bondi12 ↔ 32
    Disulfide bondi16 ↔ 34
    Disulfide bondi22 ↔ 37

    Keywords - PTMi

    Disulfide bond

    Expressioni

    Tissue specificityi

    Expressed by the venom gland.

    Structurei

    Secondary structure

    1
    38
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi10 – 2011
    Beta strandi25 – 284
    Beta strandi31 – 344

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1N8MNMR-A1-38[»]
    ProteinModelPortaliP58498.
    SMRiP58498. Positions 1-38.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP58498.

    Family & Domainsi

    Domaini

    Has the structural arrangement of an alpha-helix connected to a beta-sheet by disulfide bonds (CSalpha/beta).

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.30.30.10. 1 hit.
    InterProiIPR003614. Scorpion_toxin-like.
    IPR001947. Scorpion_toxinS_K_inh.
    [Graphical view]
    PfamiPF00451. Toxin_2. 1 hit.
    [Graphical view]
    ProDomiPD003586. Scorpion_toxinS. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF57095. SSF57095. 1 hit.
    PROSITEiPS01138. SCORP_SHORT_TOXIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P58498-1 [UniParc]FASTAAdd to Basket

    « Hide

    IEAIRCGGSR DCYRPCQKRT GCPNAKCINK TCKCYGCS                38
    Length:38
    Mass (Da):4,188
    Last modified:December 19, 2001 - v1
    Checksum:i1C1F402B8DF2EEFF
    GO

    Cross-referencesi

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1N8M NMR - A 1-38 [» ]
    ProteinModelPortali P58498.
    SMRi P58498. Positions 1-38.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P58498.

    Family and domain databases

    Gene3Di 3.30.30.10. 1 hit.
    InterProi IPR003614. Scorpion_toxin-like.
    IPR001947. Scorpion_toxinS_K_inh.
    [Graphical view ]
    Pfami PF00451. Toxin_2. 1 hit.
    [Graphical view ]
    ProDomi PD003586. Scorpion_toxinS. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF57095. SSF57095. 1 hit.
    PROSITEi PS01138. SCORP_SHORT_TOXIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Two similar peptides from the venom of the scorpion Pandinus imperator, one highly effective blocker and the other inactive on K+ channels."
      Olamendi-Portugal T., Gomez-Lagunas F., Gurrola G.B., Possani L.D.
      Toxicon 36:759-770(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE.
      Tissue: Venom.
    2. "Synthesis and characterization of Pi4, a scorpion toxin from Pandinus imperator that acts on K+ channels."
      M'Barek S., Mosbah A., Sandoz G., Fajloun Z., Olamendi-Portugal T., Rochat H., Sampieri F., Guijarro J.I., Mansuelle P., Delepierre M., De Waard M., Sabatier J.-M.
      Eur. J. Biochem. 270:3583-3592(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TOXIN TARGET, LETHAL DOSE, DISULFIDE BONDS, SYNTHESIS.
    3. "Solution structure of Pi4, a short four-disulfide-bridged scorpion toxin specific of potassium channels."
      Guijarro J.I., M'Barek S., Gomez-Lagunas F., Garnier D., Rochat H., Sabatier J.-M., Possani L.D., Delepierre M.
      Protein Sci. 12:1844-1854(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR, DISULFIDE BONDS, SYNTHESIS.

    Entry informationi

    Entry nameiKAX64_PANIM
    AccessioniPrimary (citable) accession number: P58498
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 19, 2001
    Last sequence update: December 19, 2001
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programAnimal Toxin Annotation Program

    Miscellaneousi

    Miscellaneous

    This toxin has no effect on voltage-gated potassium channel Kv1.1/KCNA1 and Kv1.3/KCNA3.1 Publication

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Scorpion potassium channel toxins
      Nomenclature of scorpion potassium channel toxins and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3