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Protein

Potassium channel toxin alpha-KTx 6.4

Gene
N/A
Organism
Pandinus imperator (Emperor scorpion)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Potently, completely and reversibly blocks voltage-gated potassium channel Kv1.2/KCNA2 and Shaker B (Sh). Also blocks small conductance (SK) calcium-activated potassium channel (KCNN).1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei10 – 101Important for the interaction with Kv1.2/KCNA2 channel
Sitei19 – 191Important for the interaction with Kv1.2/KCNA2 channel
Sitei26 – 261Basic residue of the functional dyad, important for the interaction with Kv1.2/KCNA2 channel
Sitei28 – 281Important for the interaction with Kv1.2/KCNA2 channel
Sitei30 – 301Important for the interaction with Kv1.2/KCNA2 channel
Sitei33 – 331Important for the interaction with Kv1.2/KCNA2 channel
Sitei35 – 351Aromatic residue of the functional dyad, important for the interaction with Kv1.2/KCNA2 channel

GO - Molecular functioni

  1. ion channel inhibitor activity Source: InterPro

GO - Biological processi

  1. defense response Source: InterPro
  2. pathogenesis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Calcium-activated potassium channel impairing toxin, Ion channel impairing toxin, Potassium channel impairing toxin, Toxin, Voltage-gated potassium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium channel toxin alpha-KTx 6.4
Alternative name(s):
Potassium channel-blocking toxin 4
Short name:
Pi-4
Short name:
Pi4
OrganismiPandinus imperator (Emperor scorpion)
Taxonomic identifieri55084 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesIuridaScorpionoideaScorpionidaeScorpioninaePandinus

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

LD50 is 10 µg/kg by intracerebroventricular injection into mice.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Peptidei1 – 3838Potassium channel toxin alpha-KTx 6.4PRO_0000044913Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi6 ↔ 27
Disulfide bondi12 ↔ 32
Disulfide bondi16 ↔ 34
Disulfide bondi22 ↔ 37

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

1
38
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi10 – 2011Combined sources
Beta strandi25 – 284Combined sources
Beta strandi31 – 344Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1N8MNMR-A1-38[»]
ProteinModelPortaliP58498.
SMRiP58498. Positions 1-38.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP58498.

Family & Domainsi

Domaini

Has the structural arrangement of an alpha-helix connected to a beta-sheet by disulfide bonds (CSalpha/beta).

Sequence similaritiesi

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR003614. Scorpion_toxin-like.
IPR001947. Scorpion_toxinS_K_inh.
[Graphical view]
PfamiPF00451. Toxin_2. 1 hit.
[Graphical view]
ProDomiPD003586. Scorpion_toxinS. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiPS01138. SCORP_SHORT_TOXIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P58498-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30 
IEAIRCGGSR DCYRPCQKRT GCPNAKCINK TCKCYGCS
Length:38
Mass (Da):4,188
Last modified:December 19, 2001 - v1
Checksum:i1C1F402B8DF2EEFF
GO

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1N8MNMR-A1-38[»]
ProteinModelPortaliP58498.
SMRiP58498. Positions 1-38.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP58498.

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR003614. Scorpion_toxin-like.
IPR001947. Scorpion_toxinS_K_inh.
[Graphical view]
PfamiPF00451. Toxin_2. 1 hit.
[Graphical view]
ProDomiPD003586. Scorpion_toxinS. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiPS01138. SCORP_SHORT_TOXIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Two similar peptides from the venom of the scorpion Pandinus imperator, one highly effective blocker and the other inactive on K+ channels."
    Olamendi-Portugal T., Gomez-Lagunas F., Gurrola G.B., Possani L.D.
    Toxicon 36:759-770(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
    Tissue: Venom.
  2. "Synthesis and characterization of Pi4, a scorpion toxin from Pandinus imperator that acts on K+ channels."
    M'Barek S., Mosbah A., Sandoz G., Fajloun Z., Olamendi-Portugal T., Rochat H., Sampieri F., Guijarro J.I., Mansuelle P., Delepierre M., De Waard M., Sabatier J.-M.
    Eur. J. Biochem. 270:3583-3592(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TOXIN TARGET, LETHAL DOSE, DISULFIDE BONDS, SYNTHESIS.
  3. "Solution structure of Pi4, a short four-disulfide-bridged scorpion toxin specific of potassium channels."
    Guijarro J.I., M'Barek S., Gomez-Lagunas F., Garnier D., Rochat H., Sabatier J.-M., Possani L.D., Delepierre M.
    Protein Sci. 12:1844-1854(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR, DISULFIDE BONDS, SYNTHESIS.

Entry informationi

Entry nameiKAX64_PANIM
AccessioniPrimary (citable) accession number: P58498
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 19, 2001
Last sequence update: December 19, 2001
Last modified: January 7, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Miscellaneous

This toxin has no effect on voltage-gated potassium channel Kv1.1/KCNA1 and Kv1.3/KCNA3.1 Publication

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Scorpion potassium channel toxins
    Nomenclature of scorpion potassium channel toxins and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.