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Protein

o-succinylbenzoate synthase

Gene

menC

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Converts 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC) to 2-succinylbenzoate (OSB).UniRule annotation

Catalytic activityi

(1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate = 2-succinylbenzoate + H2O.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Pathwayi: 1,4-dihydroxy-2-naphthoate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes 1,4-dihydroxy-2-naphthoate from chorismate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD)
  3. 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (menH)
  4. o-succinylbenzoate synthase (menC)
  5. 2-succinylbenzoate--CoA ligase (menE)
  6. 1,4-dihydroxy-2-naphthoyl-CoA synthase (menB)
  7. 1,4-dihydroxy-2-naphthoyl-CoA hydrolase (menI)
This subpathway is part of the pathway 1,4-dihydroxy-2-naphthoate biosynthesis, which is itself part of Quinol/quinone metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 1,4-dihydroxy-2-naphthoate from chorismate, the pathway 1,4-dihydroxy-2-naphthoate biosynthesis and in Quinol/quinone metabolism.

Pathwayi: menaquinone biosynthesis

This protein is involved in the pathway menaquinone biosynthesis, which is part of Quinol/quinone metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway menaquinone biosynthesis and in Quinol/quinone metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei133Proton donorUniRule annotation1
Metal bindingi161MagnesiumUniRule annotation1
Metal bindingi190MagnesiumUniRule annotation1
Metal bindingi213MagnesiumUniRule annotation1
Active sitei235Proton acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Menaquinone biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00079.
UPA01057; UER00165.

Names & Taxonomyi

Protein namesi
Recommended name:
o-succinylbenzoate synthaseUniRule annotation (EC:4.2.1.113UniRule annotation)
Short name:
OSB synthaseUniRule annotation
Short name:
OSBSUniRule annotation
Alternative name(s):
4-(2'-carboxyphenyl)-4-oxybutyric acid synthaseUniRule annotation
o-succinylbenzoic acid synthaseUniRule annotation
Gene namesi
Name:menCUniRule annotation
Ordered Locus Names:STM2306
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001712751 – 320o-succinylbenzoate synthaseAdd BLAST320

Proteomic databases

PaxDbiP58486.
PRIDEiP58486.

Interactioni

Protein-protein interaction databases

STRINGi99287.STM2306.

Structurei

Secondary structure

1320
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 13Combined sources12
Beta strandi18 – 21Combined sources4
Beta strandi26 – 36Combined sources11
Beta strandi39 – 45Combined sources7
Turni49 – 51Combined sources3
Helixi56 – 71Combined sources16
Helixi81 – 94Combined sources14
Helixi114 – 122Combined sources9
Beta strandi128 – 133Combined sources6
Beta strandi135 – 137Combined sources3
Helixi139 – 152Combined sources14
Beta strandi156 – 161Combined sources6
Helixi168 – 176Combined sources9
Helixi180 – 183Combined sources4
Beta strandi186 – 190Combined sources5
Beta strandi193 – 195Combined sources3
Helixi196 – 206Combined sources11
Beta strandi210 – 213Combined sources4
Helixi214 – 217Combined sources4
Beta strandi229 – 234Combined sources6
Helixi236 – 239Combined sources4
Helixi242 – 254Combined sources13
Beta strandi258 – 262Combined sources5
Helixi268 – 281Combined sources14
Helixi292 – 294Combined sources3
Beta strandi298 – 301Combined sources4
Helixi313 – 315Combined sources3
Beta strandi316 – 320Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GC2X-ray1.85A1-320[»]
ProteinModelPortaliP58486.
SMRiP58486.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP58486.

Family & Domainsi

Sequence similaritiesi

Belongs to the mandelate racemase/muconate lactonizing enzyme family. MenC type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DNI. Bacteria.
COG1441. LUCA.
HOGENOMiHOG000271247.
KOiK02549.
OMAiYEANRDG.
PhylomeDBiP58486.

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00470. MenC_1. 1 hit.
InterProiIPR029065. Enolase_C-like.
IPR029017. Enolase_N-like.
IPR013342. Mandelate_racemase_C.
IPR001354. MR/MLE/MAL.
IPR010196. OSB_synthase_MenC1.
IPR010197. OSB_synthase_MenC_2.
[Graphical view]
PANTHERiPTHR13794. PTHR13794. 1 hit.
PTHR13794:SF8. PTHR13794:SF8. 1 hit.
PfamiPF13378. MR_MLE_C. 1 hit.
[Graphical view]
SMARTiSM00922. MR_MLE. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.

Sequencei

Sequence statusi: Complete.

P58486-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSAQVYRWQ IPMDAGVVLR DRRLKTRDGL YVCLRDGERE GWGEISPLPG
60 70 80 90 100
FSQETWEEAQ TALLTWVNDW LQGSEGLPEM PSVAFGASCA LAELTGVLPE
110 120 130 140 150
AADYRAAPLC TGDPDDLVLR LADMPGEKIA KVKVGLYEAV RDGMVVNLLL
160 170 180 190 200
EAIPDLHLRL DANRAWTPLK AQQFAKYVNP DYRARIAFLE EPCKTRDDSR
210 220 230 240 250
AFARETGIAI AWDESLREAD FTFEAEEGVR AVVIKPTLTG SLDKVREQVA
260 270 280 290 300
AAHALGLTAV ISSSIESSLG LTQLARIAAW LTPGTLPGLD TLHLMQAQQI
310 320
RPWPGSALPC LKREELERLL
Length:320
Mass (Da):35,360
Last modified:December 19, 2001 - v1
Checksum:iC88260F7A06C2FD8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006468 Genomic DNA. Translation: AAL21207.1.
RefSeqiNP_461248.1. NC_003197.1.
WP_001255561.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL21207; AAL21207; STM2306.
GeneIDi1253828.
KEGGistm:STM2306.
PATRICi32383223. VBISalEnt20916_2441.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006468 Genomic DNA. Translation: AAL21207.1.
RefSeqiNP_461248.1. NC_003197.1.
WP_001255561.1. NC_003197.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GC2X-ray1.85A1-320[»]
ProteinModelPortaliP58486.
SMRiP58486.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM2306.

Proteomic databases

PaxDbiP58486.
PRIDEiP58486.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL21207; AAL21207; STM2306.
GeneIDi1253828.
KEGGistm:STM2306.
PATRICi32383223. VBISalEnt20916_2441.

Phylogenomic databases

eggNOGiENOG4105DNI. Bacteria.
COG1441. LUCA.
HOGENOMiHOG000271247.
KOiK02549.
OMAiYEANRDG.
PhylomeDBiP58486.

Enzyme and pathway databases

UniPathwayiUPA00079.
UPA01057; UER00165.

Miscellaneous databases

EvolutionaryTraceiP58486.

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00470. MenC_1. 1 hit.
InterProiIPR029065. Enolase_C-like.
IPR029017. Enolase_N-like.
IPR013342. Mandelate_racemase_C.
IPR001354. MR/MLE/MAL.
IPR010196. OSB_synthase_MenC1.
IPR010197. OSB_synthase_MenC_2.
[Graphical view]
PANTHERiPTHR13794. PTHR13794. 1 hit.
PTHR13794:SF8. PTHR13794:SF8. 1 hit.
PfamiPF13378. MR_MLE_C. 1 hit.
[Graphical view]
SMARTiSM00922. MR_MLE. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMENC_SALTY
AccessioniPrimary (citable) accession number: P58486
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 19, 2001
Last sequence update: December 19, 2001
Last modified: November 30, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.