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Protein

Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1

Gene

Ctdsp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation (By similarity). Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells.By similarity1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per monomer.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei964-aspartylphosphate intermediateBy similarity1
Metal bindingi96MagnesiumBy similarity1
Active sitei98Proton donorBy similarity1
Metal bindingi98Magnesium; via carbonyl oxygenBy similarity1
Sitei152Transition state stabilizerBy similarity1
Sitei190Transition state stabilizerBy similarity1
Metal bindingi207MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • negative regulation of G1/S transition of mitotic cell cycle Source: MGI
  • negative regulation of neurogenesis Source: MGI
  • negative regulation of neuron differentiation Source: MGI
  • negative regulation of protein phosphorylation Source: MGI
  • protein dephosphorylation Source: MGI
  • regulation of transcription by RNA polymerase II Source: MGI

Keywordsi

Molecular functionHydrolase, Protein phosphatase
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1 (EC:3.1.3.16)
Alternative name(s):
Golli-interacting protein
Short name:
GIP
Nuclear LIM interactor-interacting factor 3
Short name:
NLI-interacting factor 3
Small C-terminal domain phosphatase 1
Short name:
SCP1
Short name:
Small CTD phosphatase 1
Gene namesi
Name:Ctdsp1
Synonyms:Nif3, Nliif
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2654470 Ctdsp1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi96D → E: Leads to enhanced neuronal differentiation of stem cells; when associated with N-98. 1 Publication1
Mutagenesisi98D → N: Leads to enhanced neuronal differentiation of stem cells; when associated with E-96. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002125731 – 261Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1Add BLAST261

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP58466
PaxDbiP58466
PeptideAtlasiP58466
PRIDEiP58466

PTM databases

iPTMnetiP58466
PhosphoSitePlusiP58466
SwissPalmiP58466

Expressioni

Tissue specificityi

Expression is restricted to non-neuronal tissues.1 Publication

Gene expression databases

BgeeiENSMUSG00000026176
ExpressionAtlasiP58466 baseline and differential
GenevisibleiP58466 MM

Interactioni

Subunit structurei

Monomer. Interacts with REST.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
CTDSP1Q9GZU72EBI-7091612,EBI-751587From Homo sapiens.

Protein-protein interaction databases

BioGridi230610, 1 interactor
IntActiP58466, 2 interactors
MINTiP58466
STRINGi10090.ENSMUSP00000027367

Structurei

3D structure databases

ProteinModelPortaliP58466
SMRiP58466
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini86 – 244FCP1 homologyPROSITE-ProRule annotationAdd BLAST159

Phylogenomic databases

eggNOGiKOG1605 Eukaryota
COG5190 LUCA
GeneTreeiENSGT00390000017194
HOGENOMiHOG000236379
HOVERGENiHBG053298
InParanoidiP58466
KOiK15731
OMAiNQQHPQE
OrthoDBiEOG091G0J49
PhylomeDBiP58466
TreeFamiTF313556

Family and domain databases

Gene3Di3.40.50.1000, 1 hit
InterProiView protein in InterPro
IPR011948 Dullard_phosphatase
IPR004274 FCP1_dom
IPR036412 HAD-like_sf
IPR023214 HAD_sf
PfamiView protein in Pfam
PF03031 NIF, 1 hit
SMARTiView protein in SMART
SM00577 CPDc, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
TIGRFAMsiTIGR02251 HIF-SF_euk, 1 hit
PROSITEiView protein in PROSITE
PS50969 FCP1, 1 hit

Sequencei

Sequence statusi: Complete.

P58466-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSSAVITQI SKEEARGPLR GKGDQKSAVS QKPRSRGILH SLFCCVCRDD
60 70 80 90 100
GEPLPAHSGA PLLVEENGAI PKHTPVQYLL PEAKAQDSDK ICVVIDLDET
110 120 130 140 150
LVHSSFKPVN NADFIIPVEI DGVVHQVYVL KRPHVDEFLQ RMGELFECVL
160 170 180 190 200
FTASLAKYAD PVADLLDKWG AFRARLFRES CVFHRGNYVK DLSRLGRDLR
210 220 230 240 250
RVLILDNSPA SYVFHPDNAV PVASWFDNMS DTELHDLLPF FEQLSRVDDV
260
YSVLRQPRPG S
Length:261
Mass (Da):29,266
Last modified:December 13, 2001 - v1
Checksum:iBFB4471390FE9091
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY028804 mRNA Translation: AAK83555.1
BC065158 mRNA Translation: AAH65158.1
BC079638 mRNA Translation: AAH79638.1
CCDSiCCDS15048.1
RefSeqiNP_694728.1, NM_153088.2
UniGeneiMm.165042

Genome annotation databases

EnsembliENSMUST00000027367; ENSMUSP00000027367; ENSMUSG00000026176
GeneIDi227292
KEGGimmu:227292
UCSCiuc007blz.2 mouse

Similar proteinsi

Entry informationi

Entry nameiCTDS1_MOUSE
AccessioniPrimary (citable) accession number: P58466
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 13, 2001
Last sequence update: December 13, 2001
Last modified: March 28, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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