P58466 (CTDS1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1
EC=3.1.3.16

Alternative name(s):
Golli-interacting protein
Short name=GIP
Nuclear LIM interactor-interacting factor 3
Short name=NLI-interacting factor 3
Small C-terminal domain phosphatase 1
Short name=SCP1
Short name=Small CTD phosphatase 1

Gene names

Name:	Ctdsp1
Synonyms:	Nif3, Nliif

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation By similarity. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells. Ref.3
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 magnesium ion per monomer By similarity.
Subunit structure	Monomer By similarity. Interacts with REST By similarity.
Subcellular location	Nucleus By similarity.
Tissue specificity	Expression is restricted to non-neuronal tissues. Ref.3
Sequence similarities	Contains 1 FCP1 homology domain.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of neurogenesis Inferred from direct assay PubMed 17403776. Source: MGI negative regulation of neuron differentiation Inferred from direct assay PubMed 17403776. Source: MGI protein dephosphorylation Inferred from electronic annotation. Source: Compara regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	CTD phosphatase activity Inferred from electronic annotation. Source: Compara metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 261	261	Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1		PRO_0000212573
Regions
	Domain	86 – 244	159	FCP1 homology
Sites
	Active site	96	1	4-aspartylphosphate intermediate By similarity
	Active site	98	1	Proton donor By similarity
	Metal binding	96	1	Magnesium By similarity
	Metal binding	98	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	207	1	Magnesium By similarity
	Site	152	1	Transition state stabilizer By similarity
	Site	190	1	Transition state stabilizer By similarity
Experimental info
	Mutagenesis	96	1	D → E: Leads to enhanced neuronal differentiation of stem cells; when associated with N-98. Ref.3
	Mutagenesis	98	1	D → N: Leads to enhanced neuronal differentiation of stem cells; when associated with E-96. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P58466 [UniParc]. Last modified December 13, 2001. Version 1. Checksum: BFB4471390FE9091	FASTA	261	29,266
	10 20 30 40 50 60 MDSSAVITQI SKEEARGPLR GKGDQKSAVS QKPRSRGILH SLFCCVCRDD GEPLPAHSGA 70 80 90 100 110 120 PLLVEENGAI PKHTPVQYLL PEAKAQDSDK ICVVIDLDET LVHSSFKPVN NADFIIPVEI 130 140 150 160 170 180 DGVVHQVYVL KRPHVDEFLQ RMGELFECVL FTASLAKYAD PVADLLDKWG AFRARLFRES 190 200 210 220 230 240 CVFHRGNYVK DLSRLGRDLR RVLILDNSPA SYVFHPDNAV PVASWFDNMS DTELHDLLPF 250 260 FEQLSRVDDV YSVLRQPRPG S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The golli products of the myelin basic protein gene are associated with LIM-binding proteins in the brain." Fernandes A.O., Campagnoni C.W., Kampf K., Handley V.W., Schonmann V., Bongarzone E.R., Campagnoni A.T. Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[3]	"Small CTD phosphatases function in silencing neuronal gene expression." Yeo M., Lee S.-K., Lee B., Ruiz E.C., Pfaff S.L., Gill G.N. Science 307:596-600(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF ASP-96 AND ASP-98.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY028804 mRNA. Translation: AAK83555.1. BC065158 mRNA. Translation: AAH65158.1. BC079638 mRNA. Translation: AAH79638.1.
IPI	IPI00119725.
RefSeq	NP_694728.1. NM_153088.2.
UniGene	Mm.165042.
3D structure databases
ProteinModelPortal	P58466.
SMR	P58466. Positions 77-256.
ModBase	Search...
PTM databases
PhosphoSite	P58466.
Proteomic databases
PaxDb	P58466.
PRIDE	P58466.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000027367; ENSMUSP00000027367; ENSMUSG00000026176.
GeneID	227292.
KEGG	mmu:227292.
Organism-specific databases
CTD	58190.
MGI	MGI:2654470. Ctdsp1.
Phylogenomic databases
eggNOG	COG5190.
HOGENOM	HOG000236379.
HOVERGEN	HBG053298.
InParanoid	P58466.
KO	K15731.
OMA	PINNNAP.
OrthoDB	EOG43FGXJ.
Gene expression databases
ArrayExpress	P58466.
Bgee	P58466.
Genevestigator	P58466.
Family and domain databases
Gene3D	3.40.50.1000. 1 hit.
InterPro	IPR011948. Dullard_phosphatase. IPR023214. HAD-like_dom. IPR004274. NIF. [Graphical view]
Pfam	PF03031. NIF. 1 hit. [Graphical view]
SMART	SM00577. CPDc. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR02251. HIF-SF_euk. 1 hit.
PROSITE	PS50969. FCP1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	378540.
SOURCE	Search...

Entry information

Entry name

CTDS1_MOUSE

Accession

Primary (citable) accession number: P58466

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 13, 2001
Last sequence update:	December 13, 2001
Last modified:	April 3, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program