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Protein

Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1

Gene

Ctdsp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation (By similarity). Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells.By similarity1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per monomer.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei964-aspartylphosphate intermediateBy similarity1
Metal bindingi96MagnesiumBy similarity1
Active sitei98Proton donorBy similarity1
Metal bindingi98Magnesium; via carbonyl oxygenBy similarity1
Sitei152Transition state stabilizerBy similarity1
Sitei190Transition state stabilizerBy similarity1
Metal bindingi207MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • negative regulation of G1/S transition of mitotic cell cycle Source: MGI
  • negative regulation of neurogenesis Source: MGI
  • negative regulation of neuron differentiation Source: MGI
  • negative regulation of protein phosphorylation Source: MGI
  • protein dephosphorylation Source: MGI
  • regulation of transcription from RNA polymerase II promoter Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1 (EC:3.1.3.16)
Alternative name(s):
Golli-interacting protein
Short name:
GIP
Nuclear LIM interactor-interacting factor 3
Short name:
NLI-interacting factor 3
Small C-terminal domain phosphatase 1
Short name:
SCP1
Short name:
Small CTD phosphatase 1
Gene namesi
Name:Ctdsp1
Synonyms:Nif3, Nliif
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2654470. Ctdsp1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi96D → E: Leads to enhanced neuronal differentiation of stem cells; when associated with N-98. 1 Publication1
Mutagenesisi98D → N: Leads to enhanced neuronal differentiation of stem cells; when associated with E-96. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002125731 – 261Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1Add BLAST261

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP58466.
PaxDbiP58466.
PeptideAtlasiP58466.
PRIDEiP58466.

PTM databases

iPTMnetiP58466.
PhosphoSitePlusiP58466.
SwissPalmiP58466.

Expressioni

Tissue specificityi

Expression is restricted to non-neuronal tissues.1 Publication

Gene expression databases

BgeeiENSMUSG00000026176.
ExpressionAtlasiP58466. baseline and differential.
GenevisibleiP58466. MM.

Interactioni

Subunit structurei

Monomer. Interacts with REST.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
CTDSP1Q9GZU72EBI-7091612,EBI-751587From a different organism.

Protein-protein interaction databases

IntActiP58466. 2 interactors.
STRINGi10090.ENSMUSP00000027367.

Structurei

3D structure databases

ProteinModelPortaliP58466.
SMRiP58466.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini86 – 244FCP1 homologyPROSITE-ProRule annotationAdd BLAST159

Sequence similaritiesi

Contains 1 FCP1 homology domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1605. Eukaryota.
COG5190. LUCA.
GeneTreeiENSGT00390000017194.
HOGENOMiHOG000236379.
HOVERGENiHBG053298.
InParanoidiP58466.
KOiK15731.
OMAiPPIAPQH.
OrthoDBiEOG091G0J49.
PhylomeDBiP58466.
TreeFamiTF313556.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR011948. Dullard_phosphatase.
IPR004274. FCP1_dom.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF03031. NIF. 1 hit.
[Graphical view]
SMARTiSM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR02251. HIF-SF_euk. 1 hit.
PROSITEiPS50969. FCP1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P58466-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSSAVITQI SKEEARGPLR GKGDQKSAVS QKPRSRGILH SLFCCVCRDD
60 70 80 90 100
GEPLPAHSGA PLLVEENGAI PKHTPVQYLL PEAKAQDSDK ICVVIDLDET
110 120 130 140 150
LVHSSFKPVN NADFIIPVEI DGVVHQVYVL KRPHVDEFLQ RMGELFECVL
160 170 180 190 200
FTASLAKYAD PVADLLDKWG AFRARLFRES CVFHRGNYVK DLSRLGRDLR
210 220 230 240 250
RVLILDNSPA SYVFHPDNAV PVASWFDNMS DTELHDLLPF FEQLSRVDDV
260
YSVLRQPRPG S
Length:261
Mass (Da):29,266
Last modified:December 13, 2001 - v1
Checksum:iBFB4471390FE9091
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY028804 mRNA. Translation: AAK83555.1.
BC065158 mRNA. Translation: AAH65158.1.
BC079638 mRNA. Translation: AAH79638.1.
CCDSiCCDS15048.1.
RefSeqiNP_694728.1. NM_153088.2.
UniGeneiMm.165042.

Genome annotation databases

EnsembliENSMUST00000027367; ENSMUSP00000027367; ENSMUSG00000026176.
GeneIDi227292.
KEGGimmu:227292.
UCSCiuc007blz.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY028804 mRNA. Translation: AAK83555.1.
BC065158 mRNA. Translation: AAH65158.1.
BC079638 mRNA. Translation: AAH79638.1.
CCDSiCCDS15048.1.
RefSeqiNP_694728.1. NM_153088.2.
UniGeneiMm.165042.

3D structure databases

ProteinModelPortaliP58466.
SMRiP58466.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP58466. 2 interactors.
STRINGi10090.ENSMUSP00000027367.

PTM databases

iPTMnetiP58466.
PhosphoSitePlusiP58466.
SwissPalmiP58466.

Proteomic databases

EPDiP58466.
PaxDbiP58466.
PeptideAtlasiP58466.
PRIDEiP58466.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027367; ENSMUSP00000027367; ENSMUSG00000026176.
GeneIDi227292.
KEGGimmu:227292.
UCSCiuc007blz.2. mouse.

Organism-specific databases

CTDi58190.
MGIiMGI:2654470. Ctdsp1.

Phylogenomic databases

eggNOGiKOG1605. Eukaryota.
COG5190. LUCA.
GeneTreeiENSGT00390000017194.
HOGENOMiHOG000236379.
HOVERGENiHBG053298.
InParanoidiP58466.
KOiK15731.
OMAiPPIAPQH.
OrthoDBiEOG091G0J49.
PhylomeDBiP58466.
TreeFamiTF313556.

Miscellaneous databases

PROiP58466.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026176.
ExpressionAtlasiP58466. baseline and differential.
GenevisibleiP58466. MM.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR011948. Dullard_phosphatase.
IPR004274. FCP1_dom.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF03031. NIF. 1 hit.
[Graphical view]
SMARTiSM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR02251. HIF-SF_euk. 1 hit.
PROSITEiPS50969. FCP1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCTDS1_MOUSE
AccessioniPrimary (citable) accession number: P58466
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 13, 2001
Last sequence update: December 13, 2001
Last modified: November 2, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.