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P58466 (CTDS1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1

EC=3.1.3.16
Alternative name(s):
Golli-interacting protein
Short name=GIP
Nuclear LIM interactor-interacting factor 3
Short name=NLI-interacting factor 3
Small C-terminal domain phosphatase 1
Short name=SCP1
Short name=Small CTD phosphatase 1
Gene names
Name:Ctdsp1
Synonyms:Nif3, Nliif
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length261 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation By similarity. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells. Ref.3

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 1 magnesium ion per monomer By similarity.

Subunit structure

Monomer By similarity. Interacts with REST By similarity.

Subcellular location

Nucleus By similarity.

Tissue specificity

Expression is restricted to non-neuronal tissues. Ref.3

Sequence similarities

Contains 1 FCP1 homology domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CTDSP1Q9GZU72EBI-7091612,EBI-751587From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 261261Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1
PRO_0000212573

Regions

Domain86 – 244159FCP1 homology

Sites

Active site9614-aspartylphosphate intermediate By similarity
Active site981Proton donor By similarity
Metal binding961Magnesium By similarity
Metal binding981Magnesium; via carbonyl oxygen By similarity
Metal binding2071Magnesium By similarity
Site1521Transition state stabilizer By similarity
Site1901Transition state stabilizer By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity

Experimental info

Mutagenesis961D → E: Leads to enhanced neuronal differentiation of stem cells; when associated with N-98. Ref.3
Mutagenesis981D → N: Leads to enhanced neuronal differentiation of stem cells; when associated with E-96. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P58466 [UniParc].

Last modified December 13, 2001. Version 1.
Checksum: BFB4471390FE9091

FASTA26129,266
        10         20         30         40         50         60 
MDSSAVITQI SKEEARGPLR GKGDQKSAVS QKPRSRGILH SLFCCVCRDD GEPLPAHSGA 

        70         80         90        100        110        120 
PLLVEENGAI PKHTPVQYLL PEAKAQDSDK ICVVIDLDET LVHSSFKPVN NADFIIPVEI 

       130        140        150        160        170        180 
DGVVHQVYVL KRPHVDEFLQ RMGELFECVL FTASLAKYAD PVADLLDKWG AFRARLFRES 

       190        200        210        220        230        240 
CVFHRGNYVK DLSRLGRDLR RVLILDNSPA SYVFHPDNAV PVASWFDNMS DTELHDLLPF 

       250        260 
FEQLSRVDDV YSVLRQPRPG S 

« Hide

References

« Hide 'large scale' references
[1]"The golli products of the myelin basic protein gene are associated with LIM-binding proteins in the brain."
Fernandes A.O., Campagnoni C.W., Kampf K., Handley V.W., Schonmann V., Bongarzone E.R., Campagnoni A.T.
Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[3]"Small CTD phosphatases function in silencing neuronal gene expression."
Yeo M., Lee S.-K., Lee B., Ruiz E.C., Pfaff S.L., Gill G.N.
Science 307:596-600(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF ASP-96 AND ASP-98.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY028804 mRNA. Translation: AAK83555.1.
BC065158 mRNA. Translation: AAH65158.1.
BC079638 mRNA. Translation: AAH79638.1.
RefSeqNP_694728.1. NM_153088.2.
UniGeneMm.165042.

3D structure databases

ProteinModelPortalP58466.
SMRP58466. Positions 77-256.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP58466. 1 interaction.
STRING10090.ENSMUSP00000027367.

PTM databases

PhosphoSiteP58466.

Proteomic databases

PaxDbP58466.
PRIDEP58466.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000027367; ENSMUSP00000027367; ENSMUSG00000026176.
GeneID227292.
KEGGmmu:227292.
UCSCuc007blz.2. mouse.

Organism-specific databases

CTD58190.
MGIMGI:2654470. Ctdsp1.

Phylogenomic databases

eggNOGCOG5190.
HOGENOMHOG000236379.
HOVERGENHBG053298.
InParanoidP58466.
KOK15731.
OMAPINNNAP.
OrthoDBEOG71P2C3.
TreeFamTF313556.

Gene expression databases

ArrayExpressP58466.
BgeeP58466.
GenevestigatorP58466.

Family and domain databases

Gene3D3.40.50.1000. 1 hit.
InterProIPR011948. Dullard_phosphatase.
IPR023214. HAD-like_dom.
IPR004274. NIF.
[Graphical view]
PfamPF03031. NIF. 1 hit.
[Graphical view]
SMARTSM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMSSF56784. SSF56784. 1 hit.
TIGRFAMsTIGR02251. HIF-SF_euk. 1 hit.
PROSITEPS50969. FCP1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio378540.
PROP58466.
SOURCESearch...

Entry information

Entry nameCTDS1_MOUSE
AccessionPrimary (citable) accession number: P58466
Entry history
Integrated into UniProtKB/Swiss-Prot: December 13, 2001
Last sequence update: December 13, 2001
Last modified: March 19, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot