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Protein

Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1

Gene

Ctdsp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation (By similarity). Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells.By similarity1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per monomer.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei96 – 9614-aspartylphosphate intermediateBy similarity
Metal bindingi96 – 961MagnesiumBy similarity
Active sitei98 – 981Proton donorBy similarity
Metal bindingi98 – 981Magnesium; via carbonyl oxygenBy similarity
Sitei152 – 1521Transition state stabilizerBy similarity
Sitei190 – 1901Transition state stabilizerBy similarity
Metal bindingi207 – 2071MagnesiumBy similarity

GO - Molecular functioni

  1. CTD phosphatase activity Source: MGI
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. negative regulation of G1/S transition of mitotic cell cycle Source: MGI
  2. negative regulation of neurogenesis Source: MGI
  3. negative regulation of neuron differentiation Source: MGI
  4. negative regulation of protein phosphorylation Source: MGI
  5. protein dephosphorylation Source: MGI
  6. regulation of transcription from RNA polymerase II promoter Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1 (EC:3.1.3.16)
Alternative name(s):
Golli-interacting protein
Short name:
GIP
Nuclear LIM interactor-interacting factor 3
Short name:
NLI-interacting factor 3
Small C-terminal domain phosphatase 1
Short name:
SCP1
Short name:
Small CTD phosphatase 1
Gene namesi
Name:Ctdsp1
Synonyms:Nif3, Nliif
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2654470. Ctdsp1.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. extracellular vesicular exosome Source: MGI
  2. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi96 – 961D → E: Leads to enhanced neuronal differentiation of stem cells; when associated with N-98. 1 Publication
Mutagenesisi98 – 981D → N: Leads to enhanced neuronal differentiation of stem cells; when associated with E-96. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 261261Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1PRO_0000212573Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP58466.
PaxDbiP58466.
PRIDEiP58466.

PTM databases

PhosphoSiteiP58466.

Expressioni

Tissue specificityi

Expression is restricted to non-neuronal tissues.1 Publication

Gene expression databases

BgeeiP58466.
ExpressionAtlasiP58466. baseline and differential.
GenevestigatoriP58466.

Interactioni

Subunit structurei

Monomer. Interacts with REST.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
CTDSP1Q9GZU72EBI-7091612,EBI-751587From a different organism.

Protein-protein interaction databases

IntActiP58466. 1 interaction.
STRINGi10090.ENSMUSP00000027367.

Structurei

3D structure databases

SMRiP58466. Positions 77-256.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini86 – 244159FCP1 homologyPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 FCP1 homology domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5190.
HOGENOMiHOG000236379.
HOVERGENiHBG053298.
InParanoidiP58466.
KOiK15731.
OMAiPPIAPQH.
OrthoDBiEOG71P2C3.
PhylomeDBiP58466.
TreeFamiTF313556.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR011948. Dullard_phosphatase.
IPR023214. HAD-like_dom.
IPR004274. NIF.
[Graphical view]
PfamiPF03031. NIF. 1 hit.
[Graphical view]
SMARTiSM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR02251. HIF-SF_euk. 1 hit.
PROSITEiPS50969. FCP1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P58466-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSSAVITQI SKEEARGPLR GKGDQKSAVS QKPRSRGILH SLFCCVCRDD
60 70 80 90 100
GEPLPAHSGA PLLVEENGAI PKHTPVQYLL PEAKAQDSDK ICVVIDLDET
110 120 130 140 150
LVHSSFKPVN NADFIIPVEI DGVVHQVYVL KRPHVDEFLQ RMGELFECVL
160 170 180 190 200
FTASLAKYAD PVADLLDKWG AFRARLFRES CVFHRGNYVK DLSRLGRDLR
210 220 230 240 250
RVLILDNSPA SYVFHPDNAV PVASWFDNMS DTELHDLLPF FEQLSRVDDV
260
YSVLRQPRPG S
Length:261
Mass (Da):29,266
Last modified:December 12, 2001 - v1
Checksum:iBFB4471390FE9091
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY028804 mRNA. Translation: AAK83555.1.
BC065158 mRNA. Translation: AAH65158.1.
BC079638 mRNA. Translation: AAH79638.1.
CCDSiCCDS15048.1.
RefSeqiNP_694728.1. NM_153088.2.
UniGeneiMm.165042.

Genome annotation databases

EnsembliENSMUST00000027367; ENSMUSP00000027367; ENSMUSG00000026176.
GeneIDi227292.
KEGGimmu:227292.
UCSCiuc007blz.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY028804 mRNA. Translation: AAK83555.1.
BC065158 mRNA. Translation: AAH65158.1.
BC079638 mRNA. Translation: AAH79638.1.
CCDSiCCDS15048.1.
RefSeqiNP_694728.1. NM_153088.2.
UniGeneiMm.165042.

3D structure databases

SMRiP58466. Positions 77-256.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP58466. 1 interaction.
STRINGi10090.ENSMUSP00000027367.

PTM databases

PhosphoSiteiP58466.

Proteomic databases

MaxQBiP58466.
PaxDbiP58466.
PRIDEiP58466.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027367; ENSMUSP00000027367; ENSMUSG00000026176.
GeneIDi227292.
KEGGimmu:227292.
UCSCiuc007blz.2. mouse.

Organism-specific databases

CTDi58190.
MGIiMGI:2654470. Ctdsp1.

Phylogenomic databases

eggNOGiCOG5190.
HOGENOMiHOG000236379.
HOVERGENiHBG053298.
InParanoidiP58466.
KOiK15731.
OMAiPPIAPQH.
OrthoDBiEOG71P2C3.
PhylomeDBiP58466.
TreeFamiTF313556.

Miscellaneous databases

NextBioi378540.
PROiP58466.
SOURCEiSearch...

Gene expression databases

BgeeiP58466.
ExpressionAtlasiP58466. baseline and differential.
GenevestigatoriP58466.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR011948. Dullard_phosphatase.
IPR023214. HAD-like_dom.
IPR004274. NIF.
[Graphical view]
PfamiPF03031. NIF. 1 hit.
[Graphical view]
SMARTiSM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR02251. HIF-SF_euk. 1 hit.
PROSITEiPS50969. FCP1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The golli products of the myelin basic protein gene are associated with LIM-binding proteins in the brain."
    Fernandes A.O., Campagnoni C.W., Kampf K., Handley V.W., Schonmann V., Bongarzone E.R., Campagnoni A.T.
    Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  3. "Small CTD phosphatases function in silencing neuronal gene expression."
    Yeo M., Lee S.-K., Lee B., Ruiz E.C., Pfaff S.L., Gill G.N.
    Science 307:596-600(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF ASP-96 AND ASP-98.

Entry informationi

Entry nameiCTDS1_MOUSE
AccessioniPrimary (citable) accession number: P58466
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2001
Last sequence update: December 12, 2001
Last modified: March 31, 2015
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.