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P58466

- CTDS1_MOUSE

UniProt

P58466 - CTDS1_MOUSE

Protein

Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1

Gene

Ctdsp1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (13 Dec 2001)
      Previous versions | rss
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    Functioni

    Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation By similarity. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells.By similarity1 Publication

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 1 magnesium ion per monomer.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei96 – 9614-aspartylphosphate intermediateBy similarity
    Metal bindingi96 – 961MagnesiumBy similarity
    Active sitei98 – 981Proton donorBy similarity
    Metal bindingi98 – 981Magnesium; via carbonyl oxygenBy similarity
    Sitei152 – 1521Transition state stabilizerBy similarity
    Sitei190 – 1901Transition state stabilizerBy similarity
    Metal bindingi207 – 2071MagnesiumBy similarity

    GO - Molecular functioni

    1. CTD phosphatase activity Source: Ensembl
    2. metal ion binding Source: UniProtKB-KW
    3. protein binding Source: IntAct

    GO - Biological processi

    1. negative regulation of G1/S transition of mitotic cell cycle Source: MGI
    2. negative regulation of neurogenesis Source: MGI
    3. negative regulation of neuron differentiation Source: MGI
    4. negative regulation of protein phosphorylation Source: MGI
    5. protein dephosphorylation Source: Ensembl
    6. regulation of transcription from RNA polymerase II promoter Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1 (EC:3.1.3.16)
    Alternative name(s):
    Golli-interacting protein
    Short name:
    GIP
    Nuclear LIM interactor-interacting factor 3
    Short name:
    NLI-interacting factor 3
    Small C-terminal domain phosphatase 1
    Short name:
    SCP1
    Short name:
    Small CTD phosphatase 1
    Gene namesi
    Name:Ctdsp1
    Synonyms:Nif3, Nliif
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:2654470. Ctdsp1.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi96 – 961D → E: Leads to enhanced neuronal differentiation of stem cells; when associated with N-98. 1 Publication
    Mutagenesisi98 – 981D → N: Leads to enhanced neuronal differentiation of stem cells; when associated with E-96. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 261261Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1PRO_0000212573Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP58466.
    PaxDbiP58466.
    PRIDEiP58466.

    PTM databases

    PhosphoSiteiP58466.

    Expressioni

    Tissue specificityi

    Expression is restricted to non-neuronal tissues.1 Publication

    Gene expression databases

    ArrayExpressiP58466.
    BgeeiP58466.
    GenevestigatoriP58466.

    Interactioni

    Subunit structurei

    Monomer. Interacts with REST.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CTDSP1Q9GZU72EBI-7091612,EBI-751587From a different organism.

    Protein-protein interaction databases

    IntActiP58466. 1 interaction.
    STRINGi10090.ENSMUSP00000027367.

    Structurei

    3D structure databases

    ProteinModelPortaliP58466.
    SMRiP58466. Positions 77-256.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini86 – 244159FCP1 homologyPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 FCP1 homology domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5190.
    HOGENOMiHOG000236379.
    HOVERGENiHBG053298.
    InParanoidiP58466.
    KOiK15731.
    OMAiPINNNAP.
    OrthoDBiEOG71P2C3.
    PhylomeDBiP58466.
    TreeFamiTF313556.

    Family and domain databases

    Gene3Di3.40.50.1000. 1 hit.
    InterProiIPR011948. Dullard_phosphatase.
    IPR023214. HAD-like_dom.
    IPR004274. NIF.
    [Graphical view]
    PfamiPF03031. NIF. 1 hit.
    [Graphical view]
    SMARTiSM00577. CPDc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 1 hit.
    TIGRFAMsiTIGR02251. HIF-SF_euk. 1 hit.
    PROSITEiPS50969. FCP1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P58466-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDSSAVITQI SKEEARGPLR GKGDQKSAVS QKPRSRGILH SLFCCVCRDD    50
    GEPLPAHSGA PLLVEENGAI PKHTPVQYLL PEAKAQDSDK ICVVIDLDET 100
    LVHSSFKPVN NADFIIPVEI DGVVHQVYVL KRPHVDEFLQ RMGELFECVL 150
    FTASLAKYAD PVADLLDKWG AFRARLFRES CVFHRGNYVK DLSRLGRDLR 200
    RVLILDNSPA SYVFHPDNAV PVASWFDNMS DTELHDLLPF FEQLSRVDDV 250
    YSVLRQPRPG S 261
    Length:261
    Mass (Da):29,266
    Last modified:December 13, 2001 - v1
    Checksum:iBFB4471390FE9091
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY028804 mRNA. Translation: AAK83555.1.
    BC065158 mRNA. Translation: AAH65158.1.
    BC079638 mRNA. Translation: AAH79638.1.
    CCDSiCCDS15048.1.
    RefSeqiNP_694728.1. NM_153088.2.
    UniGeneiMm.165042.

    Genome annotation databases

    EnsembliENSMUST00000027367; ENSMUSP00000027367; ENSMUSG00000026176.
    GeneIDi227292.
    KEGGimmu:227292.
    UCSCiuc007blz.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY028804 mRNA. Translation: AAK83555.1 .
    BC065158 mRNA. Translation: AAH65158.1 .
    BC079638 mRNA. Translation: AAH79638.1 .
    CCDSi CCDS15048.1.
    RefSeqi NP_694728.1. NM_153088.2.
    UniGenei Mm.165042.

    3D structure databases

    ProteinModelPortali P58466.
    SMRi P58466. Positions 77-256.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P58466. 1 interaction.
    STRINGi 10090.ENSMUSP00000027367.

    PTM databases

    PhosphoSitei P58466.

    Proteomic databases

    MaxQBi P58466.
    PaxDbi P58466.
    PRIDEi P58466.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000027367 ; ENSMUSP00000027367 ; ENSMUSG00000026176 .
    GeneIDi 227292.
    KEGGi mmu:227292.
    UCSCi uc007blz.2. mouse.

    Organism-specific databases

    CTDi 58190.
    MGIi MGI:2654470. Ctdsp1.

    Phylogenomic databases

    eggNOGi COG5190.
    HOGENOMi HOG000236379.
    HOVERGENi HBG053298.
    InParanoidi P58466.
    KOi K15731.
    OMAi PINNNAP.
    OrthoDBi EOG71P2C3.
    PhylomeDBi P58466.
    TreeFami TF313556.

    Miscellaneous databases

    NextBioi 378540.
    PROi P58466.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P58466.
    Bgeei P58466.
    Genevestigatori P58466.

    Family and domain databases

    Gene3Di 3.40.50.1000. 1 hit.
    InterProi IPR011948. Dullard_phosphatase.
    IPR023214. HAD-like_dom.
    IPR004274. NIF.
    [Graphical view ]
    Pfami PF03031. NIF. 1 hit.
    [Graphical view ]
    SMARTi SM00577. CPDc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56784. SSF56784. 1 hit.
    TIGRFAMsi TIGR02251. HIF-SF_euk. 1 hit.
    PROSITEi PS50969. FCP1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The golli products of the myelin basic protein gene are associated with LIM-binding proteins in the brain."
      Fernandes A.O., Campagnoni C.W., Kampf K., Handley V.W., Schonmann V., Bongarzone E.R., Campagnoni A.T.
      Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    3. "Small CTD phosphatases function in silencing neuronal gene expression."
      Yeo M., Lee S.-K., Lee B., Ruiz E.C., Pfaff S.L., Gill G.N.
      Science 307:596-600(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF ASP-96 AND ASP-98.

    Entry informationi

    Entry nameiCTDS1_MOUSE
    AccessioniPrimary (citable) accession number: P58466
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 13, 2001
    Last sequence update: December 13, 2001
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3