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P58466

- CTDS1_MOUSE

UniProt

P58466 - CTDS1_MOUSE

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Protein
Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1
Gene
Ctdsp1, Nif3, Nliif
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation By similarity. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 1 magnesium ion per monomer By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei96 – 9614-aspartylphosphate intermediate By similarity
Metal bindingi96 – 961Magnesium By similarity
Active sitei98 – 981Proton donor By similarity
Metal bindingi98 – 981Magnesium; via carbonyl oxygen By similarity
Sitei152 – 1521Transition state stabilizer By similarity
Sitei190 – 1901Transition state stabilizer By similarity
Metal bindingi207 – 2071Magnesium By similarity

GO - Molecular functioni

  1. CTD phosphatase activity Source: Ensembl
  2. metal ion binding Source: UniProtKB-KW
  3. protein binding Source: IntAct

GO - Biological processi

  1. negative regulation of neurogenesis Source: MGI
  2. negative regulation of neuron differentiation Source: MGI
  3. protein dephosphorylation Source: Ensembl
  4. regulation of transcription from RNA polymerase II promoter Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1 (EC:3.1.3.16)
Alternative name(s):
Golli-interacting protein
Short name:
GIP
Nuclear LIM interactor-interacting factor 3
Short name:
NLI-interacting factor 3
Small C-terminal domain phosphatase 1
Short name:
SCP1
Short name:
Small CTD phosphatase 1
Gene namesi
Name:Ctdsp1
Synonyms:Nif3, Nliif
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:2654470. Ctdsp1.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi96 – 961D → E: Leads to enhanced neuronal differentiation of stem cells; when associated with N-98. 1 Publication
Mutagenesisi98 – 981D → N: Leads to enhanced neuronal differentiation of stem cells; when associated with E-96. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 261261Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1
PRO_0000212573Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP58466.
PaxDbiP58466.
PRIDEiP58466.

PTM databases

PhosphoSiteiP58466.

Expressioni

Tissue specificityi

Expression is restricted to non-neuronal tissues.1 Publication

Gene expression databases

ArrayExpressiP58466.
BgeeiP58466.
GenevestigatoriP58466.

Interactioni

Subunit structurei

Monomer By similarity. Interacts with REST By similarity.

Binary interactionsi

WithEntry#Exp.IntActNotes
CTDSP1Q9GZU72EBI-7091612,EBI-751587From a different organism.

Protein-protein interaction databases

IntActiP58466. 1 interaction.
STRINGi10090.ENSMUSP00000027367.

Structurei

3D structure databases

ProteinModelPortaliP58466.
SMRiP58466. Positions 77-256.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini86 – 244159FCP1 homology
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5190.
HOGENOMiHOG000236379.
HOVERGENiHBG053298.
InParanoidiP58466.
KOiK15731.
OMAiPINNNAP.
OrthoDBiEOG71P2C3.
PhylomeDBiP58466.
TreeFamiTF313556.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR011948. Dullard_phosphatase.
IPR023214. HAD-like_dom.
IPR004274. NIF.
[Graphical view]
PfamiPF03031. NIF. 1 hit.
[Graphical view]
SMARTiSM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR02251. HIF-SF_euk. 1 hit.
PROSITEiPS50969. FCP1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P58466-1 [UniParc]FASTAAdd to Basket

« Hide

MDSSAVITQI SKEEARGPLR GKGDQKSAVS QKPRSRGILH SLFCCVCRDD    50
GEPLPAHSGA PLLVEENGAI PKHTPVQYLL PEAKAQDSDK ICVVIDLDET 100
LVHSSFKPVN NADFIIPVEI DGVVHQVYVL KRPHVDEFLQ RMGELFECVL 150
FTASLAKYAD PVADLLDKWG AFRARLFRES CVFHRGNYVK DLSRLGRDLR 200
RVLILDNSPA SYVFHPDNAV PVASWFDNMS DTELHDLLPF FEQLSRVDDV 250
YSVLRQPRPG S 261
Length:261
Mass (Da):29,266
Last modified:December 13, 2001 - v1
Checksum:iBFB4471390FE9091
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY028804 mRNA. Translation: AAK83555.1.
BC065158 mRNA. Translation: AAH65158.1.
BC079638 mRNA. Translation: AAH79638.1.
CCDSiCCDS15048.1.
RefSeqiNP_694728.1. NM_153088.2.
UniGeneiMm.165042.

Genome annotation databases

EnsembliENSMUST00000027367; ENSMUSP00000027367; ENSMUSG00000026176.
GeneIDi227292.
KEGGimmu:227292.
UCSCiuc007blz.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY028804 mRNA. Translation: AAK83555.1 .
BC065158 mRNA. Translation: AAH65158.1 .
BC079638 mRNA. Translation: AAH79638.1 .
CCDSi CCDS15048.1.
RefSeqi NP_694728.1. NM_153088.2.
UniGenei Mm.165042.

3D structure databases

ProteinModelPortali P58466.
SMRi P58466. Positions 77-256.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P58466. 1 interaction.
STRINGi 10090.ENSMUSP00000027367.

PTM databases

PhosphoSitei P58466.

Proteomic databases

MaxQBi P58466.
PaxDbi P58466.
PRIDEi P58466.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000027367 ; ENSMUSP00000027367 ; ENSMUSG00000026176 .
GeneIDi 227292.
KEGGi mmu:227292.
UCSCi uc007blz.2. mouse.

Organism-specific databases

CTDi 58190.
MGIi MGI:2654470. Ctdsp1.

Phylogenomic databases

eggNOGi COG5190.
HOGENOMi HOG000236379.
HOVERGENi HBG053298.
InParanoidi P58466.
KOi K15731.
OMAi PINNNAP.
OrthoDBi EOG71P2C3.
PhylomeDBi P58466.
TreeFami TF313556.

Miscellaneous databases

NextBioi 378540.
PROi P58466.
SOURCEi Search...

Gene expression databases

ArrayExpressi P58466.
Bgeei P58466.
Genevestigatori P58466.

Family and domain databases

Gene3Di 3.40.50.1000. 1 hit.
InterProi IPR011948. Dullard_phosphatase.
IPR023214. HAD-like_dom.
IPR004274. NIF.
[Graphical view ]
Pfami PF03031. NIF. 1 hit.
[Graphical view ]
SMARTi SM00577. CPDc. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 1 hit.
TIGRFAMsi TIGR02251. HIF-SF_euk. 1 hit.
PROSITEi PS50969. FCP1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The golli products of the myelin basic protein gene are associated with LIM-binding proteins in the brain."
    Fernandes A.O., Campagnoni C.W., Kampf K., Handley V.W., Schonmann V., Bongarzone E.R., Campagnoni A.T.
    Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  3. "Small CTD phosphatases function in silencing neuronal gene expression."
    Yeo M., Lee S.-K., Lee B., Ruiz E.C., Pfaff S.L., Gill G.N.
    Science 307:596-600(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF ASP-96 AND ASP-98.

Entry informationi

Entry nameiCTDS1_MOUSE
AccessioniPrimary (citable) accession number: P58466
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 13, 2001
Last sequence update: December 13, 2001
Last modified: July 9, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi