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P58465 (CTDSL_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
CTD small phosphatase-like protein

Short name=CTDSP-like
EC=3.1.3.16
Alternative name(s):
Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 3
NIF-like protein
Nuclear LIM interactor-interacting factor 1
Short name=NLI-interacting factor 1
Small C-terminal domain phosphatase 3
Short name=SCP3
Short name=Small CTD phosphatase 3
Gene names
Name:Ctdspl
Synonyms:Nif1, Nifl
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length276 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells By similarity.

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 1 magnesium ion per monomer By similarity.

Subunit structure

Monomer By similarity. Interacts with REST By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Contains 1 FCP1 homology domain.

Ontologies

Keywords
   Cellular componentNucleus
   LigandMagnesium
Metal-binding
   Molecular functionHydrolase
Protein phosphatase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoprotein phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 276276CTD small phosphatase-like protein
PRO_0000212570

Regions

Domain102 – 260159FCP1 homology

Sites

Active site11214-aspartylphosphate intermediate By similarity
Active site1141Proton donor By similarity
Metal binding1121Magnesium By similarity
Metal binding1141Magnesium; via carbonyl oxygen By similarity
Metal binding2231Magnesium By similarity
Site1681Transition state stabilizer By similarity
Site2061Transition state stabilizer By similarity

Experimental info

Sequence conflict371Q → E in CAC69078. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P58465 [UniParc].

Last modified October 3, 2012. Version 3.
Checksum: 3989E5B2E57EEC75

FASTA27631,156
        10         20         30         40         50         60 
MDGPAIITQV TNPKEDEARS PVAGEKASQR NISLKKQRGR SILSSFFCCF RDYNVEAPPA 

        70         80         90        100        110        120 
NSPSVLPPLV EENGGLQKGD QRQVIPVPSP PAKYLLPEVT VLDYGKKCVV IDLDETLVHS 

       130        140        150        160        170        180 
SFKPISNADF IVPVEIDGTI HQVYVLKRPH VDEFLQRMGQ LFECVLFTAS LAKYADPVAD 

       190        200        210        220        230        240 
LLDRWGVFRA RLFRESCVFH RGNYVKDLSR LGRELSKVII VDNSPASYIF HPENAVPVQS 

       250        260        270 
WFDDMTDTEL LDLIPFFEGL SREDDVYSML HRLCSR 

« Hide

References

« Hide 'large scale' references
[1]"Refined physical mapping and genomic structure of a 4-Mb region (AP-20) on human chromosome 3p22-p21.33 implicated in lung and kidney cancerogenesis."
Protopopov A., Kashuba V., Zabarovsky E.
Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Colon.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ344340 mRNA. Translation: CAC69078.2.
AC055818 Genomic DNA. No translation available.
AC156800 Genomic DNA. No translation available.
BC094289 mRNA. Translation: AAH94289.1.
CCDSCCDS23605.1.
RefSeqNP_598471.3. NM_133710.3.
UniGeneMm.439928.

3D structure databases

ProteinModelPortalP58465.
SMRP58465. Positions 92-263.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000072852.

PTM databases

PhosphoSiteP58465.

Proteomic databases

PaxDbP58465.
PRIDEP58465.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000073109; ENSMUSP00000072852; ENSMUSG00000047409.
GeneID69274.
KEGGmmu:69274.
UCSCuc009rzz.2. mouse.

Organism-specific databases

CTD10217.
MGIMGI:1916524. Ctdspl.

Phylogenomic databases

eggNOGCOG5190.
GeneTreeENSGT00390000017194.
HOGENOMHOG000236379.
HOVERGENHBG053298.
InParanoidP58465.
KOK15731.
OMAHMSIITQ.
OrthoDBEOG71P2C3.
TreeFamTF313556.

Gene expression databases

ArrayExpressP58465.
GenevestigatorP58465.

Family and domain databases

Gene3D3.40.50.1000. 1 hit.
InterProIPR011948. Dullard_phosphatase.
IPR023214. HAD-like_dom.
IPR004274. NIF.
[Graphical view]
PfamPF03031. NIF. 1 hit.
[Graphical view]
SMARTSM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMSSF56784. SSF56784. 1 hit.
TIGRFAMsTIGR02251. HIF-SF_euk. 1 hit.
PROSITEPS50969. FCP1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio329009.
PROP58465.
SOURCESearch...

Entry information

Entry nameCTDSL_MOUSE
AccessionPrimary (citable) accession number: P58465
Secondary accession number(s): Q52KL5
Entry history
Integrated into UniProtKB/Swiss-Prot: December 13, 2001
Last sequence update: October 3, 2012
Last modified: July 9, 2014
This is version 81 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot