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Protein

CTD small phosphatase-like protein

Gene

Ctdspl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells (By similarity).By similarity

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per monomer.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei112 – 11214-aspartylphosphate intermediateBy similarity
Metal bindingi112 – 1121MagnesiumBy similarity
Active sitei114 – 1141Proton donorBy similarity
Metal bindingi114 – 1141Magnesium; via carbonyl oxygenBy similarity
Sitei168 – 1681Transition state stabilizerBy similarity
Sitei206 – 2061Transition state stabilizerBy similarity
Metal bindingi223 – 2231MagnesiumBy similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phosphoprotein phosphatase activity Source: UniProtKB-KW

GO - Biological processi

  1. negative regulation of G1/S transition of mitotic cell cycle Source: MGI
  2. negative regulation of protein phosphorylation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
CTD small phosphatase-like protein (EC:3.1.3.16)
Short name:
CTDSP-like
Alternative name(s):
Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 3
NIF-like protein
Nuclear LIM interactor-interacting factor 1
Short name:
NLI-interacting factor 1
Small C-terminal domain phosphatase 3
Short name:
SCP3
Short name:
Small CTD phosphatase 3
Gene namesi
Name:Ctdspl
Synonyms:Nif1, Nifl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1916524. Ctdspl.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. extracellular vesicular exosome Source: MGI
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 276276CTD small phosphatase-like proteinPRO_0000212570Add
BLAST

Proteomic databases

PaxDbiP58465.
PRIDEiP58465.

PTM databases

PhosphoSiteiP58465.

Expressioni

Gene expression databases

ExpressionAtlasiP58465. baseline and differential.
GenevestigatoriP58465.

Interactioni

Subunit structurei

Monomer. Interacts with REST.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000072852.

Structurei

3D structure databases

ProteinModelPortaliP58465.
SMRiP58465. Positions 92-263.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini102 – 260159FCP1 homologyPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 FCP1 homology domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5190.
GeneTreeiENSGT00390000017194.
HOGENOMiHOG000236379.
HOVERGENiHBG053298.
InParanoidiP58465.
KOiK15731.
OMAiLHKLCNR.
OrthoDBiEOG71P2C3.
TreeFamiTF313556.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR011948. Dullard_phosphatase.
IPR023214. HAD-like_dom.
IPR004274. NIF.
[Graphical view]
PfamiPF03031. NIF. 1 hit.
[Graphical view]
SMARTiSM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR02251. HIF-SF_euk. 1 hit.
PROSITEiPS50969. FCP1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P58465-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDGPAIITQV TNPKEDEARS PVAGEKASQR NISLKKQRGR SILSSFFCCF
60 70 80 90 100
RDYNVEAPPA NSPSVLPPLV EENGGLQKGD QRQVIPVPSP PAKYLLPEVT
110 120 130 140 150
VLDYGKKCVV IDLDETLVHS SFKPISNADF IVPVEIDGTI HQVYVLKRPH
160 170 180 190 200
VDEFLQRMGQ LFECVLFTAS LAKYADPVAD LLDRWGVFRA RLFRESCVFH
210 220 230 240 250
RGNYVKDLSR LGRELSKVII VDNSPASYIF HPENAVPVQS WFDDMTDTEL
260 270
LDLIPFFEGL SREDDVYSML HRLCSR
Length:276
Mass (Da):31,156
Last modified:October 3, 2012 - v3
Checksum:i3989E5B2E57EEC75
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti37 – 371Q → E in CAC69078 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ344340 mRNA. Translation: CAC69078.2.
AC055818 Genomic DNA. No translation available.
AC156800 Genomic DNA. No translation available.
BC094289 mRNA. Translation: AAH94289.1.
CCDSiCCDS23605.1.
RefSeqiNP_598471.3. NM_133710.3.
UniGeneiMm.439928.

Genome annotation databases

EnsembliENSMUST00000073109; ENSMUSP00000072852; ENSMUSG00000047409.
GeneIDi69274.
KEGGimmu:69274.
UCSCiuc009rzz.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ344340 mRNA. Translation: CAC69078.2.
AC055818 Genomic DNA. No translation available.
AC156800 Genomic DNA. No translation available.
BC094289 mRNA. Translation: AAH94289.1.
CCDSiCCDS23605.1.
RefSeqiNP_598471.3. NM_133710.3.
UniGeneiMm.439928.

3D structure databases

ProteinModelPortaliP58465.
SMRiP58465. Positions 92-263.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000072852.

PTM databases

PhosphoSiteiP58465.

Proteomic databases

PaxDbiP58465.
PRIDEiP58465.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000073109; ENSMUSP00000072852; ENSMUSG00000047409.
GeneIDi69274.
KEGGimmu:69274.
UCSCiuc009rzz.2. mouse.

Organism-specific databases

CTDi10217.
MGIiMGI:1916524. Ctdspl.

Phylogenomic databases

eggNOGiCOG5190.
GeneTreeiENSGT00390000017194.
HOGENOMiHOG000236379.
HOVERGENiHBG053298.
InParanoidiP58465.
KOiK15731.
OMAiLHKLCNR.
OrthoDBiEOG71P2C3.
TreeFamiTF313556.

Miscellaneous databases

NextBioi329009.
PROiP58465.
SOURCEiSearch...

Gene expression databases

ExpressionAtlasiP58465. baseline and differential.
GenevestigatoriP58465.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR011948. Dullard_phosphatase.
IPR023214. HAD-like_dom.
IPR004274. NIF.
[Graphical view]
PfamiPF03031. NIF. 1 hit.
[Graphical view]
SMARTiSM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR02251. HIF-SF_euk. 1 hit.
PROSITEiPS50969. FCP1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Refined physical mapping and genomic structure of a 4-Mb region (AP-20) on human chromosome 3p22-p21.33 implicated in lung and kidney cancerogenesis."
    Protopopov A., Kashuba V., Zabarovsky E.
    Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Colon.

Entry informationi

Entry nameiCTDSL_MOUSE
AccessioniPrimary (citable) accession number: P58465
Secondary accession number(s): Q52KL5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 13, 2001
Last sequence update: October 3, 2012
Last modified: February 4, 2015
This is version 86 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.