P58465 (CTDSL_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CTD small phosphatase-like protein
Short name=CTDSP-like
EC=3.1.3.16

Alternative name(s):
Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 3
NIF-like protein
Nuclear LIM interactor-interacting factor 1
Short name=NLI-interacting factor 1
Small C-terminal domain phosphatase 3
Short name=SCP3
Short name=Small CTD phosphatase 3

Gene names

Name:	Ctdspl
Synonyms:	Nif1, Nifl

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells By similarity.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 magnesium ion per monomer By similarity.
Subunit structure	Monomer By similarity. Interacts with REST By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Contains 1 FCP1 homology domain.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dephosphorylation Inferred from electronic annotation. Source: GOC
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 276	276	CTD small phosphatase-like protein		PRO_0000212570
Regions
	Domain	102 – 260	159	FCP1 homology
Sites
	Active site	112	1	4-aspartylphosphate intermediate By similarity
	Active site	114	1	Proton donor By similarity
	Metal binding	112	1	Magnesium By similarity
	Metal binding	114	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	223	1	Magnesium By similarity
	Site	168	1	Transition state stabilizer By similarity
	Site	206	1	Transition state stabilizer By similarity
Experimental info
	Sequence conflict	37	1	Q → E in CAC69078. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P58465 [UniParc]. Last modified October 3, 2012. Version 3. Checksum: 3989E5B2E57EEC75	FASTA	276	31,156
	10 20 30 40 50 60 MDGPAIITQV TNPKEDEARS PVAGEKASQR NISLKKQRGR SILSSFFCCF RDYNVEAPPA 70 80 90 100 110 120 NSPSVLPPLV EENGGLQKGD QRQVIPVPSP PAKYLLPEVT VLDYGKKCVV IDLDETLVHS 130 140 150 160 170 180 SFKPISNADF IVPVEIDGTI HQVYVLKRPH VDEFLQRMGQ LFECVLFTAS LAKYADPVAD 190 200 210 220 230 240 LLDRWGVFRA RLFRESCVFH RGNYVKDLSR LGRELSKVII VDNSPASYIF HPENAVPVQS 250 260 270 WFDDMTDTEL LDLIPFFEGL SREDDVYSML HRLCSR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Refined physical mapping and genomic structure of a 4-Mb region (AP-20) on human chromosome 3p22-p21.33 implicated in lung and kidney cancerogenesis." Protopopov A., Kashuba V., Zabarovsky E. Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Colon.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ344340 mRNA. Translation: CAC69078.2. AC055818 Genomic DNA. No translation available. AC156800 Genomic DNA. No translation available. BC094289 mRNA. Translation: AAH94289.1.
IPI	IPI00466513. IPI00890925.
RefSeq	NP_598471.3. NM_133710.3.
UniGene	Mm.439928.
3D structure databases
ProteinModelPortal	P58465.
SMR	P58465. Positions 92-263.
ModBase	Search...
PTM databases
PhosphoSite	P58465.
Proteomic databases
PaxDb	P58465.
PRIDE	P58465.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000073109; ENSMUSP00000072852; ENSMUSG00000047409.
GeneID	69274.
KEGG	mmu:69274.
Organism-specific databases
CTD	10217.
MGI	MGI:1916524. Ctdspl.
Phylogenomic databases
eggNOG	COG5190.
GeneTree	ENSGT00390000017194.
HOGENOM	HOG000236379.
HOVERGEN	HBG053298.
InParanoid	P58465.
KO	K15731.
OMA	MQVIPIP.
OrthoDB	EOG43FGXJ.
Gene expression databases
Genevestigator	P58465.
Family and domain databases
Gene3D	3.40.50.1000. 1 hit.
InterPro	IPR011948. Dullard_phosphatase. IPR023214. HAD-like_dom. IPR004274. NIF. [Graphical view]
Pfam	PF03031. NIF. 1 hit. [Graphical view]
SMART	SM00577. CPDc. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR02251. HIF-SF_euk. 1 hit.
PROSITE	PS50969. FCP1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	329009.
SOURCE	Search...

Entry information

Entry name

CTDSL_MOUSE

Accession

Primary (citable) accession number: P58465
Secondary accession number(s): Q52KL5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 13, 2001
Last sequence update:	October 3, 2012
Last modified:	May 1, 2013
This is version 71 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program