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P58459 (ATS10_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
A disintegrin and metalloproteinase with thrombospondin motifs 10

Short name=ADAM-TS 10
Short name=ADAM-TS10
Short name=ADAMTS-10
EC=3.4.24.-
Gene names
Name:Adamts10
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1104 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Metalloprotease that participate in microfibrils assembly. Microfibrils are extracellular matrix components occurring independently or along with elastin in the formation of elastic tissues By similarity.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subunit structure

Interacts with FBN1; this interaction promotes microfibrils assembly By similarity.

Subcellular location

Secretedextracellular spaceextracellular matrix By similarity.

Tissue specificity

Widely expressed in adult tissues. Ref.4

Developmental stage

Widely expressed throughout embryo development. Widespread expression in embryo until 12.5 days of gestation, after which it is then expressed in a more restricted fashion, with especially strong expression in developing lung, bone, and craniofacial region. Ref.4

Domain

The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix By similarity.

Post-translational modification

Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion By similarity.

Sequence similarities

Contains 1 disintegrin domain.

Contains 1 peptidase M12B domain.

Contains 1 PLAC domain.

Contains 5 TSP type-1 domains.

Sequence caution

The sequence AAI00559.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence AAI00559.1 differs from that shown. Reason: Frameshift at position 261.

The sequence AAO17380.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P58459-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P58459-2)

The sequence of this isoform differs from the canonical sequence as follows:
     146-147: HG → VS
     148-1104: Missing.
Note: May be due to intron retention. No experimental confirmation available.
Isoform 3 (identifier: P58459-3)

The sequence of this isoform differs from the canonical sequence as follows:
     80-145: Missing.
     272-279: AKLFQDSS → SAFLGLES
     280-1104: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Propeptide26 – 233208 By similarity
PRO_0000270149
Chain234 – 1104871A disintegrin and metalloproteinase with thrombospondin motifs 10
PRO_0000078211

Regions

Domain239 – 457219Peptidase M12B
Domain460 – 54687Disintegrin
Domain547 – 60256TSP type-1 1
Domain825 – 88561TSP type-1 2
Domain888 – 94356TSP type-1 3
Domain944 – 100360TSP type-1 4
Domain1004 – 105855TSP type-1 5
Domain1066 – 110439PLAC
Region706 – 818113Spacer By similarity
Compositional bias568 – 5714Poly-Ser
Compositional bias604 – 705102Cys-rich

Sites

Active site3931 By similarity
Metal binding3921Zinc; catalytic By similarity
Metal binding3961Zinc; catalytic By similarity
Metal binding4021Zinc; catalytic By similarity

Amino acid modifications

Glycosylation901N-linked (GlcNAc...) Potential
Glycosylation2221N-linked (GlcNAc...) Potential
Glycosylation7401N-linked (GlcNAc...) Potential
Glycosylation7951N-linked (GlcNAc...) Potential
Glycosylation8921N-linked (GlcNAc...) Potential
Disulfide bond315 ↔ 376 By similarity
Disulfide bond351 ↔ 358 By similarity
Disulfide bond370 ↔ 452 By similarity
Disulfide bond409 ↔ 436 By similarity
Disulfide bond479 ↔ 501 By similarity
Disulfide bond490 ↔ 508 By similarity
Disulfide bond496 ↔ 531 By similarity
Disulfide bond521 ↔ 536 By similarity
Disulfide bond559 ↔ 596 By similarity
Disulfide bond563 ↔ 601 By similarity
Disulfide bond574 ↔ 586 By similarity
Disulfide bond837 ↔ 879 By similarity
Disulfide bond841 ↔ 884 By similarity
Disulfide bond852 ↔ 866 By similarity

Natural variations

Alternative sequence80 – 14566Missing in isoform 3.
VSP_026108
Alternative sequence146 – 1472HG → VS in isoform 2.
VSP_026109
Alternative sequence148 – 1104957Missing in isoform 2.
VSP_026110
Alternative sequence272 – 2798AKLFQDSS → SAFLGLES in isoform 3.
VSP_026111
Alternative sequence280 – 1104825Missing in isoform 3.
VSP_026112

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 12, 2007. Version 3.
Checksum: 57811356FCB390FF

FASTA1,104121,086
        10         20         30         40         50         60 
MASACQILRW ALALGLGLTF KVTHAFRSQD ELLSSLESYE IAFPTRVDHN GAMLAFSPPA 

        70         80         90        100        110        120 
FRRQRRGAGA TTESRLFYKV AAPSTHFLLN LTRSPRLLAG HVSVEYWTRE GLAWQRAARA 

       130        140        150        160        170        180 
HCLYAGHLQG QAGSSHVAVS TCGGLHGLIV ADDEEYLIEP LQGGPKGHRG PEESGPHVVY 

       190        200        210        220        230        240 
KRSSLRHPHL DTACGVRDEK PWKGRPWWLR TLKPPPARPL GNESERGQLG LKRSVSRERY 

       250        260        270        280        290        300 
VETLVVADKM MVAYHGRRDV EQYVLAIMNI VAKLFQDSSL GNIVNILVTR LILLTEDQPT 

       310        320        330        340        350        360 
LEITHHAGKS LDSFCKWQKS IVSHSGHGNA IPENGVANHD TAVLITRYDI CIYKNKPCGT 

       370        380        390        400        410        420 
LGLAPVGGMC ERERSCSINE DIGLATAFTI AHEIGHTFGM NHDGVGNGCG ARGQDPAKLM 

       430        440        450        460        470        480 
AAHITMKTNP FVWSSCSRDY ITSFLDSGLG LCLNNRPPRQ DFVYPTVAPG QAYDADEQCR 

       490        500        510        520        530        540 
FQHGVKSRQC KYGEVCSELW CLSKSNRCIT NSIPAAEGTL CQTHTIDKGW CYKRVCVPFG 

       550        560        570        580        590        600 
SRPEGVDGAW GPWTPWGDCS RSCGGGVSSS SRHCDSPRPT IGGKYCLGER RRHRSCNTND 

       610        620        630        640        650        660 
CPPGSQDFRE MQCSEFDSVP FRGKFYTWKT YRGGGVKACS LTCLAEGFNF YTERAAAVVD 

       670        680        690        700        710        720 
GTPCRPDTVD ICVSGECKHV GCDRVLGSDL REDKCRVCGG DGSACETIEG VFSPALPGTG 

       730        740        750        760        770        780 
YEDVVWIPKG SVHIFIQDLN LSLSHLALKG DQESLLLEGL PGTPQPHRLP LAGTTFHLRQ 

       790        800        810        820        830        840 
GPDQAQSLEA LGPINASLII MVLAQAELPA LHYRFNAPIA RDALPPYSWH YAPWTKCSAQ 

       850        860        870        880        890        900 
CAGGSQVQVV ECRNQLDSSA VAPHYCSGHS KLPKRQRACN TEPCPPDWVV GNWSRCSRSC 

       910        920        930        940        950        960 
DAGVRSRSVV CQRRVSAAEE KALDDSACPQ PRPPVLEACQ GPMCPPEWAT LDWSECTPSC 

       970        980        990       1000       1010       1020 
GPGLRHRVVL CKSADQRSTL PPGHCLPAAK PPSTMRCNLR RCPPARWVTS EWGECSTQCG 

      1030       1040       1050       1060       1070       1080 
LGQQQRTVRC TSHTGQPSRE CTEALRPSTM QQCEAKCDSV VPPGDGPEEC KDVNKVAYCP 

      1090       1100 
LVLKFQFCSR AYFRQMCCKT CQGR 

« Hide

Isoform 2 [UniParc].

Checksum: E2833F8A51970103
Show »

FASTA14716,041
Isoform 3 [UniParc].

Checksum: 7DA18F920CAF53BF
Show »

FASTA21323,990

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6J.
Tissue: Corpora quadrigemina.
[2]"Gene content of the 750-kb critical region for mouse embryonic ectoderm lethal tcl-w5."
Abe K., Yuzuriha M., Sugimoto M., Ko M.S., Brathwaite M.E., Waeltz P., Nagaraja R.
Mamm. Genome 15:265-276(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 129/Sv.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Strain: Czech II.
Tissue: Lung.
[4]"Discovery and characterization of a novel, widely expressed metalloprotease, ADAMTS10, and its proteolytic activation."
Somerville R.P.T., Jungers K.A., Apte S.S.
J. Biol. Chem. 279:51208-51217(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 655-1104 (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK046333 mRNA. Translation: BAC32684.1.
AF528163 Genomic DNA. Translation: AAO17380.1. Sequence problems.
BC100558 mRNA. Translation: AAI00559.1. Sequence problems.
AF302012 mRNA. Translation: AAK97226.1.
RefSeqNP_766207.2. NM_172619.3.
UniGeneMm.29304.

3D structure databases

ProteinModelPortalP58459.
SMRP58459. Positions 238-820, 825-1054.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSM12.235.

PTM databases

PhosphoSiteP58459.

Proteomic databases

PaxDbP58459.
PRIDEP58459.

Protocols and materials databases

DNASU224697.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000087623; ENSMUSP00000084905; ENSMUSG00000024299. [P58459-1]
GeneID224697.
KEGGmmu:224697.
UCSCuc008bym.2. mouse. [P58459-2]
uc008byn.2. mouse. [P58459-3]
uc008byp.2. mouse. [P58459-1]

Organism-specific databases

CTD81794.
MGIMGI:2449112. Adamts10.

Phylogenomic databases

eggNOGNOG288089.
GeneTreeENSGT00750000117508.
HOGENOMHOG000004800.
HOVERGENHBG004315.
InParanoidP58459.
KOK08625.
OMAGRRDIEQ.
OrthoDBEOG78WKQV.
PhylomeDBP58459.
TreeFamTF313537.

Gene expression databases

ArrayExpressP58459.
BgeeP58459.
GenevestigatorP58459.

Family and domain databases

Gene3D3.40.390.10. 1 hit.
InterProIPR010294. ADAM_spacer1.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR013273. Peptidase_M12B_ADAM-TS.
IPR002870. Peptidase_M12B_N.
IPR010909. PLAC.
IPR000884. Thrombospondin_1_rpt.
[Graphical view]
PfamPF05986. ADAM_spacer1. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF08686. PLAC. 1 hit.
PF01421. Reprolysin. 1 hit.
PF00090. TSP_1. 5 hits.
[Graphical view]
PRINTSPR01857. ADAMTSFAMILY.
SMARTSM00209. TSP1. 5 hits.
[Graphical view]
SUPFAMSSF82895. SSF82895. 5 hits.
PROSITEPS50215. ADAM_MEPRO. 1 hit.
PS50900. PLAC. 1 hit.
PS50092. TSP1. 5 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio377312.
PROP58459.
SOURCESearch...

Entry information

Entry nameATS10_MOUSE
AccessionPrimary (citable) accession number: P58459
Secondary accession number(s): Q497H1, Q78TI1, Q8CG28
Entry history
Integrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: June 12, 2007
Last modified: April 16, 2014
This is version 107 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot