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P58459

- ATS10_MOUSE

UniProt

P58459 - ATS10_MOUSE

Protein

A disintegrin and metalloproteinase with thrombospondin motifs 10

Gene

Adamts10

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 3 (12 Jun 2007)
      Previous versions | rss
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    Functioni

    Metalloprotease that participate in microfibrils assembly. Microfibrils are extracellular matrix components occurring independently or along with elastin in the formation of elastic tissues By similarity.By similarity

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi392 – 3921Zinc; catalyticBy similarity
    Active sitei393 – 3931PROSITE-ProRule annotation
    Metal bindingi396 – 3961Zinc; catalyticBy similarity
    Metal bindingi402 – 4021Zinc; catalyticBy similarity

    GO - Molecular functioni

    1. metalloendopeptidase activity Source: InterPro
    2. zinc ion binding Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiM12.235.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    A disintegrin and metalloproteinase with thrombospondin motifs 10 (EC:3.4.24.-)
    Short name:
    ADAM-TS 10
    Short name:
    ADAM-TS10
    Short name:
    ADAMTS-10
    Gene namesi
    Name:Adamts10
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:2449112. Adamts10.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular matrix Source: UniProtKB
    2. microfibril Source: UniProtKB

    Keywords - Cellular componenti

    Extracellular matrix, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525Sequence AnalysisAdd
    BLAST
    Propeptidei26 – 233208By similarityPRO_0000270149Add
    BLAST
    Chaini234 – 1104871A disintegrin and metalloproteinase with thrombospondin motifs 10PRO_0000078211Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi90 – 901N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi222 – 2221N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi315 ↔ 376By similarity
    Disulfide bondi351 ↔ 358By similarity
    Disulfide bondi370 ↔ 452By similarity
    Disulfide bondi409 ↔ 436By similarity
    Disulfide bondi479 ↔ 501By similarity
    Disulfide bondi490 ↔ 508By similarity
    Disulfide bondi496 ↔ 531By similarity
    Disulfide bondi521 ↔ 536By similarity
    Disulfide bondi559 ↔ 596By similarity
    Disulfide bondi563 ↔ 601By similarity
    Disulfide bondi574 ↔ 586By similarity
    Glycosylationi740 – 7401N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi795 – 7951N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi837 ↔ 879By similarity
    Disulfide bondi841 ↔ 884By similarity
    Disulfide bondi852 ↔ 866By similarity
    Glycosylationi892 – 8921N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion By similarity.By similarity

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiP58459.
    PRIDEiP58459.

    PTM databases

    PhosphoSiteiP58459.

    Expressioni

    Tissue specificityi

    Widely expressed in adult tissues.1 Publication

    Developmental stagei

    Widely expressed throughout embryo development. Widespread expression in embryo until 12.5 days of gestation, after which it is then expressed in a more restricted fashion, with especially strong expression in developing lung, bone, and craniofacial region.1 Publication

    Gene expression databases

    ArrayExpressiP58459.
    BgeeiP58459.
    GenevestigatoriP58459.

    Interactioni

    Subunit structurei

    Interacts with FBN1; this interaction promotes microfibrils assembly.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP58459.
    SMRiP58459. Positions 236-749.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini239 – 457219Peptidase M12BPROSITE-ProRule annotationAdd
    BLAST
    Domaini460 – 54687DisintegrinAdd
    BLAST
    Domaini547 – 60256TSP type-1 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini825 – 88561TSP type-1 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini888 – 94356TSP type-1 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini944 – 100360TSP type-1 4PROSITE-ProRule annotationAdd
    BLAST
    Domaini1004 – 105855TSP type-1 5PROSITE-ProRule annotationAdd
    BLAST
    Domaini1066 – 110439PLACPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni706 – 818113SpacerBy similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi568 – 5714Poly-Ser
    Compositional biasi604 – 705102Cys-richAdd
    BLAST

    Domaini

    The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.By similarity

    Sequence similaritiesi

    Contains 1 disintegrin domain.Curated
    Contains 1 peptidase M12B domain.PROSITE-ProRule annotation
    Contains 1 PLAC domain.PROSITE-ProRule annotation
    Contains 5 TSP type-1 domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG288089.
    GeneTreeiENSGT00750000117508.
    HOGENOMiHOG000004800.
    HOVERGENiHBG004315.
    InParanoidiP58459.
    KOiK08625.
    OMAiGRRDIEQ.
    OrthoDBiEOG78WKQV.
    PhylomeDBiP58459.
    TreeFamiTF313537.

    Family and domain databases

    Gene3Di3.40.390.10. 1 hit.
    InterProiIPR010294. ADAM_spacer1.
    IPR024079. MetalloPept_cat_dom.
    IPR001590. Peptidase_M12B.
    IPR013273. Peptidase_M12B_ADAM-TS.
    IPR002870. Peptidase_M12B_N.
    IPR010909. PLAC.
    IPR000884. Thrombospondin_1_rpt.
    [Graphical view]
    PfamiPF05986. ADAM_spacer1. 1 hit.
    PF01562. Pep_M12B_propep. 1 hit.
    PF08686. PLAC. 1 hit.
    PF01421. Reprolysin. 1 hit.
    PF00090. TSP_1. 5 hits.
    [Graphical view]
    PRINTSiPR01857. ADAMTSFAMILY.
    SMARTiSM00209. TSP1. 5 hits.
    [Graphical view]
    SUPFAMiSSF82895. SSF82895. 5 hits.
    PROSITEiPS50215. ADAM_MEPRO. 1 hit.
    PS50900. PLAC. 1 hit.
    PS50092. TSP1. 5 hits.
    PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P58459-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MASACQILRW ALALGLGLTF KVTHAFRSQD ELLSSLESYE IAFPTRVDHN     50
    GAMLAFSPPA FRRQRRGAGA TTESRLFYKV AAPSTHFLLN LTRSPRLLAG 100
    HVSVEYWTRE GLAWQRAARA HCLYAGHLQG QAGSSHVAVS TCGGLHGLIV 150
    ADDEEYLIEP LQGGPKGHRG PEESGPHVVY KRSSLRHPHL DTACGVRDEK 200
    PWKGRPWWLR TLKPPPARPL GNESERGQLG LKRSVSRERY VETLVVADKM 250
    MVAYHGRRDV EQYVLAIMNI VAKLFQDSSL GNIVNILVTR LILLTEDQPT 300
    LEITHHAGKS LDSFCKWQKS IVSHSGHGNA IPENGVANHD TAVLITRYDI 350
    CIYKNKPCGT LGLAPVGGMC ERERSCSINE DIGLATAFTI AHEIGHTFGM 400
    NHDGVGNGCG ARGQDPAKLM AAHITMKTNP FVWSSCSRDY ITSFLDSGLG 450
    LCLNNRPPRQ DFVYPTVAPG QAYDADEQCR FQHGVKSRQC KYGEVCSELW 500
    CLSKSNRCIT NSIPAAEGTL CQTHTIDKGW CYKRVCVPFG SRPEGVDGAW 550
    GPWTPWGDCS RSCGGGVSSS SRHCDSPRPT IGGKYCLGER RRHRSCNTND 600
    CPPGSQDFRE MQCSEFDSVP FRGKFYTWKT YRGGGVKACS LTCLAEGFNF 650
    YTERAAAVVD GTPCRPDTVD ICVSGECKHV GCDRVLGSDL REDKCRVCGG 700
    DGSACETIEG VFSPALPGTG YEDVVWIPKG SVHIFIQDLN LSLSHLALKG 750
    DQESLLLEGL PGTPQPHRLP LAGTTFHLRQ GPDQAQSLEA LGPINASLII 800
    MVLAQAELPA LHYRFNAPIA RDALPPYSWH YAPWTKCSAQ CAGGSQVQVV 850
    ECRNQLDSSA VAPHYCSGHS KLPKRQRACN TEPCPPDWVV GNWSRCSRSC 900
    DAGVRSRSVV CQRRVSAAEE KALDDSACPQ PRPPVLEACQ GPMCPPEWAT 950
    LDWSECTPSC GPGLRHRVVL CKSADQRSTL PPGHCLPAAK PPSTMRCNLR 1000
    RCPPARWVTS EWGECSTQCG LGQQQRTVRC TSHTGQPSRE CTEALRPSTM 1050
    QQCEAKCDSV VPPGDGPEEC KDVNKVAYCP LVLKFQFCSR AYFRQMCCKT 1100
    CQGR 1104
    Length:1,104
    Mass (Da):121,086
    Last modified:June 12, 2007 - v3
    Checksum:i57811356FCB390FF
    GO
    Isoform 2 (identifier: P58459-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         146-147: HG → VS
         148-1104: Missing.

    Note: May be due to intron retention. No experimental confirmation available.

    Show »
    Length:147
    Mass (Da):16,041
    Checksum:iE2833F8A51970103
    GO
    Isoform 3 (identifier: P58459-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         80-145: Missing.
         272-279: AKLFQDSS → SAFLGLES
         280-1104: Missing.

    Show »
    Length:213
    Mass (Da):23,990
    Checksum:i7DA18F920CAF53BF
    GO

    Sequence cautioni

    The sequence AAI00559.1 differs from that shown. Reason: Frameshift at position 261.
    The sequence AAI00559.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
    The sequence AAO17380.1 differs from that shown. Reason: Erroneous gene model prediction.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei80 – 14566Missing in isoform 3. 1 PublicationVSP_026108Add
    BLAST
    Alternative sequencei146 – 1472HG → VS in isoform 2. 1 PublicationVSP_026109
    Alternative sequencei148 – 1104957Missing in isoform 2. 1 PublicationVSP_026110Add
    BLAST
    Alternative sequencei272 – 2798AKLFQDSS → SAFLGLES in isoform 3. 1 PublicationVSP_026111
    Alternative sequencei280 – 1104825Missing in isoform 3. 1 PublicationVSP_026112Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK046333 mRNA. Translation: BAC32684.1.
    AF528163 Genomic DNA. Translation: AAO17380.1. Sequence problems.
    BC100558 mRNA. Translation: AAI00559.1. Sequence problems.
    AF302012 mRNA. Translation: AAK97226.1.
    CCDSiCCDS37566.1. [P58459-1]
    RefSeqiNP_766207.2. NM_172619.3. [P58459-1]
    UniGeneiMm.29304.

    Genome annotation databases

    EnsembliENSMUST00000087623; ENSMUSP00000084905; ENSMUSG00000024299. [P58459-1]
    GeneIDi224697.
    KEGGimmu:224697.
    UCSCiuc008bym.2. mouse. [P58459-2]
    uc008byn.2. mouse. [P58459-3]
    uc008byp.2. mouse. [P58459-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK046333 mRNA. Translation: BAC32684.1 .
    AF528163 Genomic DNA. Translation: AAO17380.1 . Sequence problems.
    BC100558 mRNA. Translation: AAI00559.1 . Sequence problems.
    AF302012 mRNA. Translation: AAK97226.1 .
    CCDSi CCDS37566.1. [P58459-1 ]
    RefSeqi NP_766207.2. NM_172619.3. [P58459-1 ]
    UniGenei Mm.29304.

    3D structure databases

    ProteinModelPortali P58459.
    SMRi P58459. Positions 236-749.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi M12.235.

    PTM databases

    PhosphoSitei P58459.

    Proteomic databases

    PaxDbi P58459.
    PRIDEi P58459.

    Protocols and materials databases

    DNASUi 224697.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000087623 ; ENSMUSP00000084905 ; ENSMUSG00000024299 . [P58459-1 ]
    GeneIDi 224697.
    KEGGi mmu:224697.
    UCSCi uc008bym.2. mouse. [P58459-2 ]
    uc008byn.2. mouse. [P58459-3 ]
    uc008byp.2. mouse. [P58459-1 ]

    Organism-specific databases

    CTDi 81794.
    MGIi MGI:2449112. Adamts10.

    Phylogenomic databases

    eggNOGi NOG288089.
    GeneTreei ENSGT00750000117508.
    HOGENOMi HOG000004800.
    HOVERGENi HBG004315.
    InParanoidi P58459.
    KOi K08625.
    OMAi GRRDIEQ.
    OrthoDBi EOG78WKQV.
    PhylomeDBi P58459.
    TreeFami TF313537.

    Miscellaneous databases

    NextBioi 377312.
    PROi P58459.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P58459.
    Bgeei P58459.
    Genevestigatori P58459.

    Family and domain databases

    Gene3Di 3.40.390.10. 1 hit.
    InterProi IPR010294. ADAM_spacer1.
    IPR024079. MetalloPept_cat_dom.
    IPR001590. Peptidase_M12B.
    IPR013273. Peptidase_M12B_ADAM-TS.
    IPR002870. Peptidase_M12B_N.
    IPR010909. PLAC.
    IPR000884. Thrombospondin_1_rpt.
    [Graphical view ]
    Pfami PF05986. ADAM_spacer1. 1 hit.
    PF01562. Pep_M12B_propep. 1 hit.
    PF08686. PLAC. 1 hit.
    PF01421. Reprolysin. 1 hit.
    PF00090. TSP_1. 5 hits.
    [Graphical view ]
    PRINTSi PR01857. ADAMTSFAMILY.
    SMARTi SM00209. TSP1. 5 hits.
    [Graphical view ]
    SUPFAMi SSF82895. SSF82895. 5 hits.
    PROSITEi PS50215. ADAM_MEPRO. 1 hit.
    PS50900. PLAC. 1 hit.
    PS50092. TSP1. 5 hits.
    PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6J.
      Tissue: Corpora quadrigemina.
    2. "Gene content of the 750-kb critical region for mouse embryonic ectoderm lethal tcl-w5."
      Abe K., Yuzuriha M., Sugimoto M., Ko M.S., Brathwaite M.E., Waeltz P., Nagaraja R.
      Mamm. Genome 15:265-276(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 129/Sv.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
      Strain: Czech II.
      Tissue: Lung.
    4. "Discovery and characterization of a novel, widely expressed metalloprotease, ADAMTS10, and its proteolytic activation."
      Somerville R.P.T., Jungers K.A., Apte S.S.
      J. Biol. Chem. 279:51208-51217(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 655-1104 (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiATS10_MOUSE
    AccessioniPrimary (citable) accession number: P58459
    Secondary accession number(s): Q497H1, Q78TI1, Q8CG28
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 5, 2001
    Last sequence update: June 12, 2007
    Last modified: October 1, 2014
    This is version 111 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3