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P58459

- ATS10_MOUSE

UniProt

P58459 - ATS10_MOUSE

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Protein

A disintegrin and metalloproteinase with thrombospondin motifs 10

Gene

Adamts10

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Metalloprotease that participate in microfibrils assembly. Microfibrils are extracellular matrix components occurring independently or along with elastin in the formation of elastic tissues (By similarity).By similarity

Cofactori

Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi392 – 3921Zinc; catalyticBy similarity
Active sitei393 – 3931PROSITE-ProRule annotation
Metal bindingi396 – 3961Zinc; catalyticBy similarity
Metal bindingi402 – 4021Zinc; catalyticBy similarity

GO - Molecular functioni

  1. metalloendopeptidase activity Source: InterPro
  2. zinc ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM12.235.

Names & Taxonomyi

Protein namesi
Recommended name:
A disintegrin and metalloproteinase with thrombospondin motifs 10 (EC:3.4.24.-)
Short name:
ADAM-TS 10
Short name:
ADAM-TS10
Short name:
ADAMTS-10
Gene namesi
Name:Adamts10
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:2449112. Adamts10.

Subcellular locationi

GO - Cellular componenti

  1. extracellular matrix Source: UniProtKB
  2. microfibril Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Propeptidei26 – 233208By similarityPRO_0000270149Add
BLAST
Chaini234 – 1104871A disintegrin and metalloproteinase with thrombospondin motifs 10PRO_0000078211Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi90 – 901N-linked (GlcNAc...)Sequence Analysis
Glycosylationi222 – 2221N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi315 ↔ 376By similarity
Disulfide bondi351 ↔ 358By similarity
Disulfide bondi370 ↔ 452By similarity
Disulfide bondi409 ↔ 436By similarity
Disulfide bondi479 ↔ 501By similarity
Disulfide bondi490 ↔ 508By similarity
Disulfide bondi496 ↔ 531By similarity
Disulfide bondi521 ↔ 536By similarity
Disulfide bondi559 ↔ 596By similarity
Disulfide bondi563 ↔ 601By similarity
Disulfide bondi574 ↔ 586By similarity
Glycosylationi740 – 7401N-linked (GlcNAc...)Sequence Analysis
Glycosylationi795 – 7951N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi837 ↔ 879By similarity
Disulfide bondi841 ↔ 884By similarity
Disulfide bondi852 ↔ 866By similarity
Glycosylationi892 – 8921N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion (By similarity).By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP58459.
PRIDEiP58459.

PTM databases

PhosphoSiteiP58459.

Expressioni

Tissue specificityi

Widely expressed in adult tissues.1 Publication

Developmental stagei

Widely expressed throughout embryo development. Widespread expression in embryo until 12.5 days of gestation, after which it is then expressed in a more restricted fashion, with especially strong expression in developing lung, bone, and craniofacial region.1 Publication

Gene expression databases

BgeeiP58459.
ExpressionAtlasiP58459. baseline and differential.
GenevestigatoriP58459.

Interactioni

Subunit structurei

Interacts with FBN1; this interaction promotes microfibrils assembly.By similarity

Structurei

3D structure databases

ProteinModelPortaliP58459.
SMRiP58459. Positions 236-749.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini239 – 457219Peptidase M12BPROSITE-ProRule annotationAdd
BLAST
Domaini460 – 54687DisintegrinAdd
BLAST
Domaini547 – 60256TSP type-1 1PROSITE-ProRule annotationAdd
BLAST
Domaini825 – 88561TSP type-1 2PROSITE-ProRule annotationAdd
BLAST
Domaini888 – 94356TSP type-1 3PROSITE-ProRule annotationAdd
BLAST
Domaini944 – 100360TSP type-1 4PROSITE-ProRule annotationAdd
BLAST
Domaini1004 – 105855TSP type-1 5PROSITE-ProRule annotationAdd
BLAST
Domaini1066 – 110439PLACPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni706 – 818113SpacerBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi568 – 5714Poly-Ser
Compositional biasi604 – 705102Cys-richAdd
BLAST

Domaini

The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.By similarity

Sequence similaritiesi

Contains 1 disintegrin domain.Curated
Contains 1 peptidase M12B domain.PROSITE-ProRule annotation
Contains 1 PLAC domain.PROSITE-ProRule annotation
Contains 5 TSP type-1 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG288089.
GeneTreeiENSGT00760000118880.
HOGENOMiHOG000004800.
HOVERGENiHBG004315.
InParanoidiP58459.
KOiK08625.
OMAiGRRDIEQ.
OrthoDBiEOG78WKQV.
PhylomeDBiP58459.
TreeFamiTF313537.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
InterProiIPR010294. ADAM_spacer1.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR013273. Peptidase_M12B_ADAM-TS.
IPR002870. Peptidase_M12B_N.
IPR010909. PLAC.
IPR000884. Thrombospondin_1_rpt.
[Graphical view]
PfamiPF05986. ADAM_spacer1. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF08686. PLAC. 1 hit.
PF01421. Reprolysin. 1 hit.
PF00090. TSP_1. 5 hits.
[Graphical view]
PRINTSiPR01857. ADAMTSFAMILY.
SMARTiSM00209. TSP1. 5 hits.
[Graphical view]
SUPFAMiSSF82895. SSF82895. 5 hits.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS50900. PLAC. 1 hit.
PS50092. TSP1. 5 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P58459-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASACQILRW ALALGLGLTF KVTHAFRSQD ELLSSLESYE IAFPTRVDHN
60 70 80 90 100
GAMLAFSPPA FRRQRRGAGA TTESRLFYKV AAPSTHFLLN LTRSPRLLAG
110 120 130 140 150
HVSVEYWTRE GLAWQRAARA HCLYAGHLQG QAGSSHVAVS TCGGLHGLIV
160 170 180 190 200
ADDEEYLIEP LQGGPKGHRG PEESGPHVVY KRSSLRHPHL DTACGVRDEK
210 220 230 240 250
PWKGRPWWLR TLKPPPARPL GNESERGQLG LKRSVSRERY VETLVVADKM
260 270 280 290 300
MVAYHGRRDV EQYVLAIMNI VAKLFQDSSL GNIVNILVTR LILLTEDQPT
310 320 330 340 350
LEITHHAGKS LDSFCKWQKS IVSHSGHGNA IPENGVANHD TAVLITRYDI
360 370 380 390 400
CIYKNKPCGT LGLAPVGGMC ERERSCSINE DIGLATAFTI AHEIGHTFGM
410 420 430 440 450
NHDGVGNGCG ARGQDPAKLM AAHITMKTNP FVWSSCSRDY ITSFLDSGLG
460 470 480 490 500
LCLNNRPPRQ DFVYPTVAPG QAYDADEQCR FQHGVKSRQC KYGEVCSELW
510 520 530 540 550
CLSKSNRCIT NSIPAAEGTL CQTHTIDKGW CYKRVCVPFG SRPEGVDGAW
560 570 580 590 600
GPWTPWGDCS RSCGGGVSSS SRHCDSPRPT IGGKYCLGER RRHRSCNTND
610 620 630 640 650
CPPGSQDFRE MQCSEFDSVP FRGKFYTWKT YRGGGVKACS LTCLAEGFNF
660 670 680 690 700
YTERAAAVVD GTPCRPDTVD ICVSGECKHV GCDRVLGSDL REDKCRVCGG
710 720 730 740 750
DGSACETIEG VFSPALPGTG YEDVVWIPKG SVHIFIQDLN LSLSHLALKG
760 770 780 790 800
DQESLLLEGL PGTPQPHRLP LAGTTFHLRQ GPDQAQSLEA LGPINASLII
810 820 830 840 850
MVLAQAELPA LHYRFNAPIA RDALPPYSWH YAPWTKCSAQ CAGGSQVQVV
860 870 880 890 900
ECRNQLDSSA VAPHYCSGHS KLPKRQRACN TEPCPPDWVV GNWSRCSRSC
910 920 930 940 950
DAGVRSRSVV CQRRVSAAEE KALDDSACPQ PRPPVLEACQ GPMCPPEWAT
960 970 980 990 1000
LDWSECTPSC GPGLRHRVVL CKSADQRSTL PPGHCLPAAK PPSTMRCNLR
1010 1020 1030 1040 1050
RCPPARWVTS EWGECSTQCG LGQQQRTVRC TSHTGQPSRE CTEALRPSTM
1060 1070 1080 1090 1100
QQCEAKCDSV VPPGDGPEEC KDVNKVAYCP LVLKFQFCSR AYFRQMCCKT

CQGR
Length:1,104
Mass (Da):121,086
Last modified:June 12, 2007 - v3
Checksum:i57811356FCB390FF
GO
Isoform 2 (identifier: P58459-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     146-147: HG → VS
     148-1104: Missing.

Note: May be due to intron retention. No experimental confirmation available.

Show »
Length:147
Mass (Da):16,041
Checksum:iE2833F8A51970103
GO
Isoform 3 (identifier: P58459-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     80-145: Missing.
     272-279: AKLFQDSS → SAFLGLES
     280-1104: Missing.

Show »
Length:213
Mass (Da):23,990
Checksum:i7DA18F920CAF53BF
GO

Sequence cautioni

The sequence AAI00559.1 differs from that shown. Reason: Frameshift at position 261.
The sequence AAI00559.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
The sequence AAO17380.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei80 – 14566Missing in isoform 3. 1 PublicationVSP_026108Add
BLAST
Alternative sequencei146 – 1472HG → VS in isoform 2. 1 PublicationVSP_026109
Alternative sequencei148 – 1104957Missing in isoform 2. 1 PublicationVSP_026110Add
BLAST
Alternative sequencei272 – 2798AKLFQDSS → SAFLGLES in isoform 3. 1 PublicationVSP_026111
Alternative sequencei280 – 1104825Missing in isoform 3. 1 PublicationVSP_026112Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK046333 mRNA. Translation: BAC32684.1.
AF528163 Genomic DNA. Translation: AAO17380.1. Sequence problems.
BC100558 mRNA. Translation: AAI00559.1. Sequence problems.
AF302012 mRNA. Translation: AAK97226.1.
CCDSiCCDS37566.1. [P58459-1]
RefSeqiNP_766207.2. NM_172619.3. [P58459-1]
UniGeneiMm.29304.

Genome annotation databases

EnsembliENSMUST00000087623; ENSMUSP00000084905; ENSMUSG00000024299. [P58459-1]
GeneIDi224697.
KEGGimmu:224697.
UCSCiuc008bym.2. mouse. [P58459-2]
uc008byn.2. mouse. [P58459-3]
uc008byp.2. mouse. [P58459-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK046333 mRNA. Translation: BAC32684.1 .
AF528163 Genomic DNA. Translation: AAO17380.1 . Sequence problems.
BC100558 mRNA. Translation: AAI00559.1 . Sequence problems.
AF302012 mRNA. Translation: AAK97226.1 .
CCDSi CCDS37566.1. [P58459-1 ]
RefSeqi NP_766207.2. NM_172619.3. [P58459-1 ]
UniGenei Mm.29304.

3D structure databases

ProteinModelPortali P58459.
SMRi P58459. Positions 236-749.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi M12.235.

PTM databases

PhosphoSitei P58459.

Proteomic databases

PaxDbi P58459.
PRIDEi P58459.

Protocols and materials databases

DNASUi 224697.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000087623 ; ENSMUSP00000084905 ; ENSMUSG00000024299 . [P58459-1 ]
GeneIDi 224697.
KEGGi mmu:224697.
UCSCi uc008bym.2. mouse. [P58459-2 ]
uc008byn.2. mouse. [P58459-3 ]
uc008byp.2. mouse. [P58459-1 ]

Organism-specific databases

CTDi 81794.
MGIi MGI:2449112. Adamts10.

Phylogenomic databases

eggNOGi NOG288089.
GeneTreei ENSGT00760000118880.
HOGENOMi HOG000004800.
HOVERGENi HBG004315.
InParanoidi P58459.
KOi K08625.
OMAi GRRDIEQ.
OrthoDBi EOG78WKQV.
PhylomeDBi P58459.
TreeFami TF313537.

Miscellaneous databases

NextBioi 377312.
PROi P58459.
SOURCEi Search...

Gene expression databases

Bgeei P58459.
ExpressionAtlasi P58459. baseline and differential.
Genevestigatori P58459.

Family and domain databases

Gene3Di 3.40.390.10. 1 hit.
InterProi IPR010294. ADAM_spacer1.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR013273. Peptidase_M12B_ADAM-TS.
IPR002870. Peptidase_M12B_N.
IPR010909. PLAC.
IPR000884. Thrombospondin_1_rpt.
[Graphical view ]
Pfami PF05986. ADAM_spacer1. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF08686. PLAC. 1 hit.
PF01421. Reprolysin. 1 hit.
PF00090. TSP_1. 5 hits.
[Graphical view ]
PRINTSi PR01857. ADAMTSFAMILY.
SMARTi SM00209. TSP1. 5 hits.
[Graphical view ]
SUPFAMi SSF82895. SSF82895. 5 hits.
PROSITEi PS50215. ADAM_MEPRO. 1 hit.
PS50900. PLAC. 1 hit.
PS50092. TSP1. 5 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Corpora quadrigemina.
  2. "Gene content of the 750-kb critical region for mouse embryonic ectoderm lethal tcl-w5."
    Abe K., Yuzuriha M., Sugimoto M., Ko M.S., Brathwaite M.E., Waeltz P., Nagaraja R.
    Mamm. Genome 15:265-276(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 129/Sv.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Strain: Czech II.
    Tissue: Lung.
  4. "Discovery and characterization of a novel, widely expressed metalloprotease, ADAMTS10, and its proteolytic activation."
    Somerville R.P.T., Jungers K.A., Apte S.S.
    J. Biol. Chem. 279:51208-51217(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 655-1104 (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiATS10_MOUSE
AccessioniPrimary (citable) accession number: P58459
Secondary accession number(s): Q497H1, Q78TI1, Q8CG28
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: June 12, 2007
Last modified: October 29, 2014
This is version 112 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3