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Protein

Kalata-B7

Gene

OAK3

Organism
Oldenlandia affinis
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probably participates in a plant defense mechanism. Has hemolytic activity.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cytolysis, Hemolysis, Plant defense

Names & Taxonomyi

Protein namesi
Recommended name:
Kalata-B7
Gene namesi
Name:OAK3
OrganismiOldenlandia affinis
Taxonomic identifieri60225 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsGentianalesRubiaceaeRubioideaeSpermacoceaeHedyotis-Oldenlandia complexOldenlandia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Sequence analysisAdd
BLAST
Propeptidei29 – 75471 PublicationPRO_0000006632Add
BLAST
Peptidei76 – 10429Kalata-B7PRO_0000006633Add
BLAST
Propeptidei105 – 1117PRO_0000006634

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki76 ↔ 104Cyclopeptide (Gly-Asn)
Disulfide bondi80 ↔ 94PROSITE-ProRule annotation
Disulfide bondi84 ↔ 96PROSITE-ProRule annotation
Disulfide bondi89 ↔ 101PROSITE-ProRule annotation

Post-translational modificationi

Kalata-B7 is a cyclic peptide.

Keywords - PTMi

Disulfide bond

Structurei

Secondary structure

1
111
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi76 – 783Combined sources
Beta strandi85 – 873Combined sources
Beta strandi90 – 934Combined sources
Beta strandi95 – 973Combined sources
Beta strandi100 – 1056Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2JWMNMR-A80-109[»]
2M9ONMR-A76-104[»]
2MW0NMR-A76-96[»]
ProteinModelPortaliP58457.
SMRiP58457. Positions 80-107.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP58457.

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.By similarity

Sequence similaritiesi

Belongs to the cyclotide family. Moebius subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Knottin, Signal

Family and domain databases

InterProiIPR005535. Cyclotide.
IPR012324. Cyclotide_moebius_CS.
[Graphical view]
PfamiPF03784. Cyclotide. 1 hit.
[Graphical view]
SUPFAMiSSF57038. SSF57038. 1 hit.
PROSITEiPS51052. CYCLOTIDE. 1 hit.
PS60009. CYCLOTIDE_MOEBIUS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P58457-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKFTNCLAL CLLLAAVVGA FGVELSEADK SAVVNEIAEK MALQEMLDGV
60 70 80 90 100
DKLFLRKMKS SETTLTMFLK EMQLKGLPVC GETCTLGTCY TQGCTCSWPI
110
CKRNGLPDVA A
Length:111
Mass (Da):11,959
Last modified:December 5, 2001 - v1
Checksum:iC0DA8E645B1F8D0B
GO

Mass spectrometryi

Molecular mass is 3071.6 Da from positions 76 - 104. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF393827 mRNA. Translation: AAL05479.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF393827 mRNA. Translation: AAL05479.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2JWMNMR-A80-109[»]
2M9ONMR-A76-104[»]
2MW0NMR-A76-96[»]
ProteinModelPortaliP58457.
SMRiP58457. Positions 80-107.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP58457.

Family and domain databases

InterProiIPR005535. Cyclotide.
IPR012324. Cyclotide_moebius_CS.
[Graphical view]
PfamiPF03784. Cyclotide. 1 hit.
[Graphical view]
SUPFAMiSSF57038. SSF57038. 1 hit.
PROSITEiPS51052. CYCLOTIDE. 1 hit.
PS60009. CYCLOTIDE_MOEBIUS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Biosynthesis and insecticidal properties of plant cyclotides: the cyclic knotted proteins from Oldenlandia affinis."
    Jennings C.V., West J., Waine C., Craik D.J., Anderson M.A.
    Proc. Natl. Acad. Sci. U.S.A. 98:10614-10619(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The cyclotide fingerprint in Oldenlandia affinis: elucidation of chemically modified, linear and novel macrocyclic peptides."
    Plan M.R.R., Goeransson U., Clark R.J., Daly N.L., Colgrave M.L., Craik D.J.
    ChemBioChem 8:1001-1011(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 76-104, MASS SPECTROMETRY.

Entry informationi

Entry nameiKAB7_OLDAF
AccessioniPrimary (citable) accession number: P58457
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: December 5, 2001
Last modified: April 13, 2016
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.