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Protein

Cycloviolacin-O11

Gene

Voc2

Organism
Viola odorata (Sweet violet)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probably participates in a plant defense mechanism.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Plant defense

Names & Taxonomyi

Protein namesi
Recommended name:
Cycloviolacin-O11
Alternative name(s):
Cyclotide c2
Gene namesi
Name:Voc2
OrganismiViola odorata (Sweet violet)
Taxonomic identifieri97441 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesViolaceaeViola

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Propeptidei23 – 8462PRO_0000294938Add
BLAST
Peptidei85 – 11531Cycloviolacin-O11PRO_0000044704Add
BLAST
Propeptidei116 – 1183PRO_0000294939

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki85 ↔ 115Cyclopeptide (Gly-Asn)
Disulfide bondi89 ↔ 105
Disulfide bondi93 ↔ 107
Disulfide bondi98 ↔ 112

Post-translational modificationi

Cycloviolacin-O11 is a cyclic peptide.

Keywords - PTMi

Disulfide bond

Structurei

3D structure databases

ProteinModelPortaliP58443.
SMRiP58443. Positions 89-117.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Sequence similaritiesi

Belongs to the cyclotide family. Bracelet subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Knottin, Signal

Family and domain databases

InterProiIPR005535. Cyclotide.
IPR012323. Cyclotide_bracelet_CS.
[Graphical view]
PfamiPF03784. Cyclotide. 1 hit.
[Graphical view]
SUPFAMiSSF57038. SSF57038. 1 hit.
PROSITEiPS51052. CYCLOTIDE. 1 hit.
PS60008. CYCLOTIDE_BRACELET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P58443-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEMKNMVVGL FLIAAFALPA LATSFEKDFI THETVQAILK KVGPSSNGML
60 70 80 90 100
DEQAISALTG KTIISNPVLE EALLTHSNSI NALGGTLPCG ESCVWIPCIS
110
AVVGCSCKSK VCYKNSLA
Length:118
Mass (Da):12,404
Last modified:July 10, 2007 - v2
Checksum:iCADD4E1C663A46BD
GO

Mass spectrometryi

Molecular mass is 3209.5 Da from positions 85 - 115. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY630563 mRNA. Translation: AAU04392.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY630563 mRNA. Translation: AAU04392.1.

3D structure databases

ProteinModelPortaliP58443.
SMRiP58443. Positions 89-117.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR005535. Cyclotide.
IPR012323. Cyclotide_bracelet_CS.
[Graphical view]
PfamiPF03784. Cyclotide. 1 hit.
[Graphical view]
SUPFAMiSSF57038. SSF57038. 1 hit.
PROSITEiPS51052. CYCLOTIDE. 1 hit.
PS60008. CYCLOTIDE_BRACELET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Conserved structural and sequence elements implicated in the processing of gene-encoded circular proteins."
    Dutton J.L., Renda R.F., Waine C., Clark R.J., Daly N.L., Jennings C.V., Anderson M.A., Craik D.J.
    J. Biol. Chem. 279:46858-46867(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Leaf.
  2. "Plant cyclotides: a unique family of cyclic and knotted proteins that defines the cyclic cystine knot structural motif."
    Craik D.J., Daly N.L., Bond T., Waine C.
    J. Mol. Biol. 294:1327-1336(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 85-115.
  3. "A novel suite of cyclotides from Viola odorata: sequence variation and the implications for structure, function and stability."
    Ireland D.C., Colgrave M.L., Craik D.J.
    Biochem. J. 400:1-12(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 85-115, MASS SPECTROMETRY.

Entry informationi

Entry nameiCYO11_VIOOD
AccessioniPrimary (citable) accession number: P58443
Secondary accession number(s): Q5USP0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: July 10, 2007
Last modified: December 9, 2015
This is version 44 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.