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Protein

ATP-binding cassette sub-family G member 8

Gene

Abcg8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

ABCG5 and ABCG8 form an obligate heterodimer that mediates Mg2+- and ATP-dependent sterol transport across the cell membrane. Plays an essential role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile. Required for normal sterol homeostasis. The heterodimer with ABCG5 has ATPase activity.By similarity

Caution

Seems to have a defective ATP-binding region.Curated

Cofactori

Mg2+By similarity

GO - Molecular functioni

GO - Biological processi

  • cholesterol efflux Source: Ensembl
  • cholesterol homeostasis Source: Ensembl
  • excretion Source: Ensembl
  • phospholipid transport Source: Ensembl
  • response to drug Source: RGD
  • response to nutrient Source: RGD

Keywordsi

Biological processLipid transport, Transport
LigandMagnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-1369062 ABC transporters in lipid homeostasis

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-binding cassette sub-family G member 8
Alternative name(s):
Sterolin-2
Gene namesi
Name:Abcg8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 6

Organism-specific databases

RGDi620300 Abcg8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 437CytoplasmicBy similarityAdd BLAST437
Transmembranei438 – 458Helical; Name=1By similarityAdd BLAST21
Topological domaini459 – 468ExtracellularBy similarity10
Transmembranei469 – 489Helical; Name=2By similarityAdd BLAST21
Topological domaini490 – 518CytoplasmicBy similarityAdd BLAST29
Transmembranei519 – 539Helical; Name=3By similarityAdd BLAST21
Topological domaini540 – 548ExtracellularBy similarity9
Transmembranei549 – 569Helical; Name=4By similarityAdd BLAST21
Topological domaini570 – 576CytoplasmicBy similarity7
Transmembranei577 – 597Helical; Name=5By similarityAdd BLAST21
Topological domaini598 – 660ExtracellularBy similarityAdd BLAST63
Transmembranei661 – 681Helical; Name=6By similarityAdd BLAST21
Topological domaini682 – 694CytoplasmicBy similarityAdd BLAST13

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000933981 – 694ATP-binding cassette sub-family G member 8Add BLAST694

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi640N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

N-glycosylated. N-glycosylation is important for efficient export out of the endoplasmic reticulum.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP58428
PRIDEiP58428

Expressioni

Tissue specificityi

Highest expression in liver, with lower levels in small intestine and colon.1 Publication

Gene expression databases

BgeeiENSRNOG00000005420
GenevisibleiP58428 RN

Interactioni

Subunit structurei

Heterodimer with ABCG8.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000058587

Structurei

3D structure databases

ProteinModelPortaliP58428
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini91 – 335ABC transporterPROSITE-ProRule annotationAdd BLAST245
Domaini436 – 684ABC transmembrane type-2Add BLAST249

Domaini

A functional Walker motif (consensus sequence G-X-X-G-X-G-K-[ST]-T) is expected to bind ATP. The essential Lys in this region is not conserved in ABCG8 (G-S-S-G-C-R-A-S) and is not required for transport activity mediated by the heterodimer with ABCG5.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0061 Eukaryota
COG1131 LUCA
GeneTreeiENSGT00740000114855
HOVERGENiHBG050444
InParanoidiP58428
KOiK05684
OMAiLLPTFHM
OrthoDBiEOG091G0E38
PhylomeDBiP58428

Family and domain databases

InterProiView protein in InterPro
IPR013525 ABC_2_trans
IPR003439 ABC_transporter-like
IPR017871 ABC_transporter_CS
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF01061 ABC2_membrane, 1 hit
PF00005 ABC_tran, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00211 ABC_TRANSPORTER_1, 1 hit
PS50893 ABC_TRANSPORTER_2, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 3 (identifier: P58428-3) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEKTKEETQ LWNGTVLQDA SSLQDSVFSS ESDNSLYFTY SGQSNTLEVR
60 70 80 90 100
DLTYQGGTCL RSWGQEDPHM SLGLSESVDM ASQVPWFEQL AQFKLPWRSR
110 120 130 140 150
GSQDSWDLGI RNLSFKVRSG QMLAIIGSAG CGRATLLDVI TGRDHGGKMK
160 170 180 190 200
SGQIWINGQP STPQLIQKCV AHVRQQDQLL PNLTVRETLT FIAQMRLPKT
210 220 230 240 250
FSQAQRDKRV EDVIAELRLR QCANTRVGNT YVRGVSGGER RRVSIGVQLL
260 270 280 290 300
WNPGILILDE PTSGLDSFTA HNLVRTLSRL AKGNRLVLIS LHQPRSDIFR
310 320 330 340 350
LFDLVLLMTS GTPIYLGVAQ HMVQYFTSIG YPCPRYSNPA DFYVDLTSID
360 370 380 390 400
RRSKEQEVAT MEKARLLAAL FLEKVQGFDD FLWKAEAKSL DTGTYAVSQT
410 420 430 440 450
LTQDTNCGTA AELPGMIQQF TTLIRRQISN DFRDLPTLFI HGAEACLMSL
460 470 480 490 500
IIGFLYYGHA DKPLSFMDMA ALLFMIGALI PFNVILDVVS KCHSERSLLY
510 520 530 540 550
YELEDGLYTA GPYFFAKVLG ELPEHCAYVI IYGMPIYWLT NLRPGPELFL
560 570 580 590 600
LHFMLLWLVV FCCRTMALAA SAMLPTFHMS SFCCNALYNS FYLTAGFMIN
610 620 630 640 650
LNNLWIVPAW ISKMSFLRWC FSGLMQIQFN GHIYTTQIGN LTFSVPGDAM
660 670 680 690
VTAMDLNSHP LYAIYLIVIG ISCGFLSLYY LSLKFIKQKS IQDW
Length:694
Mass (Da):78,236
Last modified:October 31, 2003 - v2
Checksum:i67F67C195F417587
GO
Isoform 1 (identifier: P58428-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     56-77: Missing.

Show »
Length:672
Mass (Da):75,907
Checksum:i2FE0846E71BD9D47
GO
Isoform 2 (identifier: P58428-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     56-77: Missing.
     398-398: Missing.

Note: No experimental confirmation available.
Show »
Length:671
Mass (Da):75,820
Checksum:i5BD06E61C14763FD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3 – 4EK → QT in AAK85393 (PubMed:12783625).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00876756 – 77Missing in isoform 1 and isoform 2. 1 PublicationAdd BLAST22
Alternative sequenceiVSP_000054398Missing in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF351785 mRNA Translation: AAK84831.2
AY145899 Genomic DNA Translation: AAN64276.1
AF404109 Genomic DNA Translation: AAK85393.1
RefSeqiNP_569098.2, NM_130414.2 [P58428-1]
UniGeneiRn.161892

Genome annotation databases

EnsembliENSRNOT00000007638; ENSRNOP00000007638; ENSRNOG00000005420 [P58428-1]
ENSRNOT00000061871; ENSRNOP00000058587; ENSRNOG00000005420 [P58428-3]
GeneIDi155192
KEGGirno:155192
UCSCiRGD:620300 rat [P58428-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiABCG8_RAT
AccessioniPrimary (citable) accession number: P58428
Secondary accession number(s): Q8CIQ5, Q923R7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: October 31, 2003
Last modified: May 23, 2018
This is version 117 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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