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Protein

ATP-binding cassette sub-family G member 8

Gene

Abcg8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transporter that appears to play an indispensable role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile.

GO - Molecular functioni

  • ATPase activity, coupled to transmembrane movement of substances Source: GO_Central
  • ATP binding Source: Ensembl
  • cholesterol transporter activity Source: RGD
  • sterol transporter activity Source: RGD

GO - Biological processi

  • cholesterol efflux Source: GO_Central
  • cholesterol homeostasis Source: Ensembl
  • cholesterol transport Source: RGD
  • excretion Source: Ensembl
  • negative regulation of intestinal cholesterol absorption Source: Ensembl
  • negative regulation of intestinal phytosterol absorption Source: Ensembl
  • phospholipid transport Source: Ensembl
  • regulation of intestinal cholesterol absorption Source: RGD
  • response to drug Source: RGD
  • response to nutrient Source: RGD
  • sterol transport Source: RGD
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiR-RNO-1369062. ABC transporters in lipid homeostasis.
R-RNO-265473. Trafficking of dietary sterols.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-binding cassette sub-family G member 8
Alternative name(s):
Sterolin-2
Gene namesi
Name:Abcg8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 6

Organism-specific databases

RGDi620300. Abcg8.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 434434CytoplasmicSequence analysisAdd
BLAST
Transmembranei435 – 45521Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini456 – 46813ExtracellularSequence analysisAdd
BLAST
Transmembranei469 – 48921Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini490 – 51728CytoplasmicSequence analysisAdd
BLAST
Transmembranei518 – 53821Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini539 – 5479ExtracellularSequence analysis
Transmembranei548 – 56821Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini569 – 59022CytoplasmicSequence analysisAdd
BLAST
Transmembranei591 – 61121Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini612 – 65039ExtracellularSequence analysisAdd
BLAST
Transmembranei651 – 67121Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini672 – 69423CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • apical plasma membrane Source: GO_Central
  • ATP-binding cassette (ABC) transporter complex Source: RGD
  • receptor complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 694694ATP-binding cassette sub-family G member 8PRO_0000093398Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi640 – 6401N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP58428.
PRIDEiP58428.

Expressioni

Tissue specificityi

Highest expression in liver, with lower levels in small intestine and colon.1 Publication

Gene expression databases

BgeeiENSRNOG00000005420.
GenevisibleiP58428. RN.

Interactioni

Subunit structurei

May form heterodimers with ABCG5 or be tightly coupled to ABCG5 along a pathway regulating diatery-sterol absorption and excretion.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000058587.

Structurei

3D structure databases

ProteinModelPortaliP58428.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini91 – 335245ABC transporterPROSITE-ProRule annotationAdd
BLAST
Domaini436 – 684249ABC transmembrane type-2Add
BLAST

Sequence similaritiesi

Contains 1 ABC transporter domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0061. Eukaryota.
COG1131. LUCA.
GeneTreeiENSGT00740000114855.
HOVERGENiHBG050444.
InParanoidiP58428.
KOiK05684.
OMAiDVVSKCH.
OrthoDBiEOG091G0E38.
PhylomeDBiP58428.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR013525. ABC_2_trans.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01061. ABC2_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 3 (identifier: P58428-3) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEKTKEETQ LWNGTVLQDA SSLQDSVFSS ESDNSLYFTY SGQSNTLEVR
60 70 80 90 100
DLTYQGGTCL RSWGQEDPHM SLGLSESVDM ASQVPWFEQL AQFKLPWRSR
110 120 130 140 150
GSQDSWDLGI RNLSFKVRSG QMLAIIGSAG CGRATLLDVI TGRDHGGKMK
160 170 180 190 200
SGQIWINGQP STPQLIQKCV AHVRQQDQLL PNLTVRETLT FIAQMRLPKT
210 220 230 240 250
FSQAQRDKRV EDVIAELRLR QCANTRVGNT YVRGVSGGER RRVSIGVQLL
260 270 280 290 300
WNPGILILDE PTSGLDSFTA HNLVRTLSRL AKGNRLVLIS LHQPRSDIFR
310 320 330 340 350
LFDLVLLMTS GTPIYLGVAQ HMVQYFTSIG YPCPRYSNPA DFYVDLTSID
360 370 380 390 400
RRSKEQEVAT MEKARLLAAL FLEKVQGFDD FLWKAEAKSL DTGTYAVSQT
410 420 430 440 450
LTQDTNCGTA AELPGMIQQF TTLIRRQISN DFRDLPTLFI HGAEACLMSL
460 470 480 490 500
IIGFLYYGHA DKPLSFMDMA ALLFMIGALI PFNVILDVVS KCHSERSLLY
510 520 530 540 550
YELEDGLYTA GPYFFAKVLG ELPEHCAYVI IYGMPIYWLT NLRPGPELFL
560 570 580 590 600
LHFMLLWLVV FCCRTMALAA SAMLPTFHMS SFCCNALYNS FYLTAGFMIN
610 620 630 640 650
LNNLWIVPAW ISKMSFLRWC FSGLMQIQFN GHIYTTQIGN LTFSVPGDAM
660 670 680 690
VTAMDLNSHP LYAIYLIVIG ISCGFLSLYY LSLKFIKQKS IQDW
Length:694
Mass (Da):78,236
Last modified:October 31, 2003 - v2
Checksum:i67F67C195F417587
GO
Isoform 1 (identifier: P58428-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     56-77: Missing.

Show »
Length:672
Mass (Da):75,907
Checksum:i2FE0846E71BD9D47
GO
Isoform 2 (identifier: P58428-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     56-77: Missing.
     398-398: Missing.

Note: No experimental confirmation available.
Show »
Length:671
Mass (Da):75,820
Checksum:i5BD06E61C14763FD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti3 – 42EK → QT in AAK85393 (PubMed:12783625).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei56 – 7722Missing in isoform 1 and isoform 2. 1 PublicationVSP_008767Add
BLAST
Alternative sequencei398 – 3981Missing in isoform 2. 1 PublicationVSP_000054

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF351785 mRNA. Translation: AAK84831.2.
AY145899 Genomic DNA. Translation: AAN64276.1.
AF404109 Genomic DNA. Translation: AAK85393.1.
RefSeqiNP_569098.2. NM_130414.2. [P58428-1]
UniGeneiRn.161892.

Genome annotation databases

EnsembliENSRNOT00000007638; ENSRNOP00000007638; ENSRNOG00000005420. [P58428-1]
ENSRNOT00000061871; ENSRNOP00000058587; ENSRNOG00000005420. [P58428-3]
GeneIDi155192.
KEGGirno:155192.
UCSCiRGD:620300. rat. [P58428-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF351785 mRNA. Translation: AAK84831.2.
AY145899 Genomic DNA. Translation: AAN64276.1.
AF404109 Genomic DNA. Translation: AAK85393.1.
RefSeqiNP_569098.2. NM_130414.2. [P58428-1]
UniGeneiRn.161892.

3D structure databases

ProteinModelPortaliP58428.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000058587.

Proteomic databases

PaxDbiP58428.
PRIDEiP58428.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000007638; ENSRNOP00000007638; ENSRNOG00000005420. [P58428-1]
ENSRNOT00000061871; ENSRNOP00000058587; ENSRNOG00000005420. [P58428-3]
GeneIDi155192.
KEGGirno:155192.
UCSCiRGD:620300. rat. [P58428-3]

Organism-specific databases

CTDi64241.
RGDi620300. Abcg8.

Phylogenomic databases

eggNOGiKOG0061. Eukaryota.
COG1131. LUCA.
GeneTreeiENSGT00740000114855.
HOVERGENiHBG050444.
InParanoidiP58428.
KOiK05684.
OMAiDVVSKCH.
OrthoDBiEOG091G0E38.
PhylomeDBiP58428.

Enzyme and pathway databases

ReactomeiR-RNO-1369062. ABC transporters in lipid homeostasis.
R-RNO-265473. Trafficking of dietary sterols.

Miscellaneous databases

PROiP58428.

Gene expression databases

BgeeiENSRNOG00000005420.
GenevisibleiP58428. RN.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR013525. ABC_2_trans.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01061. ABC2_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiABCG8_RAT
AccessioniPrimary (citable) accession number: P58428
Secondary accession number(s): Q8CIQ5, Q923R7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: October 31, 2003
Last modified: September 7, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Seems to have a defective ATP-binding region.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.