P58407 (MDH_SULTO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	STK_18110

Organism

Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) [Complete proteome] [HAMAP]

Taxonomic identifier

273063 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus › Sulfolobus tokodaii

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_00487
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_00487
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	Probable malate dehydrogenase HAMAP-Rule MF_00487		PRO_0000113494
Regions
	Nucleotide binding	8 – 13	6	NAD By similarity
	Nucleotide binding	118 – 120	3	NAD By similarity
Sites
	Active site	172	1	Proton acceptor By similarity
	Binding site	33	1	NAD By similarity
	Binding site	82	1	Substrate By similarity
	Binding site	88	1	Substrate By similarity
	Binding site	95	1	NAD By similarity
	Binding site	120	1	Substrate By similarity
	Binding site	148	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P58407 [UniParc]. Last modified November 16, 2001. Version 1. Checksum: 6ECE66B30DD1ACEE	FASTA	308	34,244
	10 20 30 40 50 60 MTKIAFIGVG KIGQTIAFNT IMDGYADEVM IYDIIPELPE KFEHELRHAL ASKRLKVELL 70 80 90 100 110 120 STNNLDDVAG ADIVVITAGK PRKPGMSRRD LFVDNAKIMM DLANKLPKKN HGAVYIMVSN 130 140 150 160 170 180 PVDMMASVFA RYSREFVIST GDQVETMRLR AYIAKKLKIP VYRVNGFVGG EHGEDAVVLW 190 200 210 220 230 240 STVTVNGKPF SEDLGVTKAE VEDYVKKIPG EIIRVMGGTT WGPGTIIAEL IRAVALNENK 250 260 270 280 290 300 VMSIATPRQF EDEIIHVSVP TVVGSSIGPS LENLLDEKDR WNLMASMKDF YNVYKENLKH LETSIQAQ

References

[1]

"Complete genome sequence of an aerobic thermoacidophilic Crenarchaeon, Sulfolobus tokodaii strain7." Kawarabayasi Y., Hino Y., Horikawa H., Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., Nishijima K., Otsuka R., Nakazawa H., Takamiya M., Kato Y., Yoshizawa T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Masuda S., Yanagii M., Nishimura M., Yamagishi A., Oshima T., Kikuchi H. DNA Res. 8:123-140(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 16993 / JCM 10545 / NBRC 100140 / 7.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000023 Genomic DNA. Translation: BAB66902.2.
RefSeq	NP_377793.1. NC_003106.2.
3D structure databases
ProteinModelPortal	P58407.
ModBase	Search...
Protein-protein interaction databases
STRING	273063.ST1811.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB66902; BAB66902; STK_18110.
GeneID	1459867.
KEGG	sto:ST1811.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0039.
HOGENOM	HOG000213793.
KO	K00024.
ProtClustDB	CLSK883804.
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.110.10. 1 hit.
HAMAP	MF_00487. Malate_dehydrog_3. Divergent sequence.
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11540. PTHR11540. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MDH_SULTO

Accession

Primary (citable) accession number: P58407

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	November 16, 2001
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program