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P58391 (KCNN3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Small conductance calcium-activated potassium channel protein 3

Short name=SK3
Short name=SKCa 3
Short name=SKCa3
Alternative name(s):
KCa2.3
Gene names
Name:Kcnn3
Synonyms:Sk3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length732 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Forms a voltage-independent potassium channel activated by intracellular calcium. Activation is followed by membrane hyperpolarization. Thought to regulate neuronal excitability by contributing to the slow component of synaptic afterhyperpolarization. The channel is blocked by apamin By similarity.

Subunit structure

Heterooligomer. The complex is composed of 4 channel subunits each of which binds to a calmodulin subunit which regulates the channel activity through calcium-binding By similarity.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Expressed at low levels in atrial and ventricular myocytes (at protein level). Ref.5

Sequence similarities

Belongs to the potassium channel KCNN family. KCa2.3/KCNN3 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 732732Small conductance calcium-activated potassium channel protein 3
PRO_0000155014

Regions

Transmembrane289 – 30921Helical; Name=Segment S1; Potential
Transmembrane316 – 33621Helical; Name=Segment S2; Potential
Transmembrane367 – 38721Helical; Name=Segment S3; Potential
Transmembrane406 – 42621Helical; Name=Segment S4; Potential
Transmembrane455 – 47521Helical; Name=Segment S5; Potential
Intramembrane495 – 51521Pore-forming; Name=Segment H5; Potential
Transmembrane524 – 54421Helical; Name=Segment S6; Potential
Region562 – 63877Calmodulin-binding By similarity
Compositional bias19 – 6143Pro-rich
Compositional bias30 – 9566Gln-rich
Compositional bias684 – 6885Poly-Gln
Compositional bias728 – 7314Poly-Ser

Experimental info

Sequence conflict801Missing in AAK48902. Ref.1
Sequence conflict3651L → F in AAK48902. Ref.1
Sequence conflict5741A → T in AAK48902. Ref.1
Sequence conflict5831I → V in AAK48902. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P58391 [UniParc].

Last modified October 16, 2013. Version 2.
Checksum: 62347E83FFE9DC9B

FASTA73281,333
        10         20         30         40         50         60 
MDTSGHFHDS GVGDLDEDPK CPCPSSGDEQ QQQQQPPPPP APPAVPQQPP GPLLQPQPPQ 

        70         80         90        100        110        120 
PQQQQSQQQQ QQQSQQQQQQ APLHPLPQLA QLQSQLVHPG LLHSSPTAFR APTSANSTAI 

       130        140        150        160        170        180 
LHPSSRQGSQ LNLNDHLLGH SPSSTATSGP GGGSRHRQAS PLVHRRDSNP FTEIAMSSCK 

       190        200        210        220        230        240 
YSGGVMKPLS RLSASRRNLI EAEPEGQPLQ LFSPSNPPEI IISSREDNHA HQTLLHHPNA 

       250        260        270        280        290        300 
THNHQHAGTT AGSTTFPKAN KRKNQNIGYK LGHRRALFEK RKRLSDYALI FGMFGIVVMV 

       310        320        330        340        350        360 
IETELSWGLY SKDSMFSLAL KCLISLSTVI LLGLIIAYHT REVQLFVIDN GADDWRIAMT 

       370        380        390        400        410        420 
YERILYISLE MLVCAIHPIP GEYKFFWTAR LAFSYTPSRA EADVDIILSI PMFLRLYLIA 

       430        440        450        460        470        480 
RVMLLHSKLF TDASSRSIGA LNKINFNTRF VMKTLMTICP GTVLLVFSIS LWIIAAWTVR 

       490        500        510        520        530        540 
VCERYHDQQD VTSNFLGAMW LISITFLSIG YGDMVPHTYC GKGVCLLTGI MGAGCTALVV 

       550        560        570        580        590        600 
AVVARKLELT KAEKHVHNFM MDTQLTKRIK NAAANVLRET WLIYKHTKLL KKIDHAKVRK 

       610        620        630        640        650        660 
HQRKFLQAIH QLRGVKMEQR KLSDQANTLV DLSKMQNVMY DLITELNDRS EDLEKQIGSL 

       670        680        690        700        710        720 
ESKLEHLTAS FNSLPLLIAD TLRQQQQQLL TAFVEARGIS VAVGTSHAPP SDSPIGISST 

       730 
SFPTPYTSSS SC 

« Hide

References

« Hide 'large scale' references
[1]"Molecular properties of small-conductance Ca2+-activated K+ channels expressed in murine colonic smooth muscle."
Ro S., Hatton W.J., Koh S.D., Horowitz B.
Am. J. Physiol. 281:G964-G973(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Colon.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Skin.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"Differential expression of small-conductance Ca2+-activated K+ channels SK1, SK2, and SK3 in mouse atrial and ventricular myocytes."
Tuteja D., Xu D., Timofeyev V., Lu L., Sharma D., Zhang Z., Xu Y., Nie L., Vazquez A.E., Young J.N., Glatter K.A., Chiamvimonvat N.
Am. J. Physiol. 289:H2714-H2723(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF357241 mRNA. Translation: AAK48902.1.
AK137738 mRNA. Translation: BAE23484.1.
AC166364 Genomic DNA. No translation available.
AC171273 Genomic DNA. No translation available.
BC106769 mRNA. Translation: AAI06770.1.
BC106770 mRNA. Translation: AAI06771.1.
CCDSCCDS38495.1.
RefSeqNP_536714.2. NM_080466.2.
UniGeneMm.120250.

3D structure databases

ProteinModelPortalP58391.
SMRP58391. Positions 473-675.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP58391. 2 interactions.
MINTMINT-4099712.
STRING10090.ENSMUSP00000000811.

PTM databases

PhosphoSiteP58391.

Proteomic databases

PaxDbP58391.
PRIDEP58391.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000000811; ENSMUSP00000000811; ENSMUSG00000000794.
GeneID140493.
KEGGmmu:140493.
UCSCuc012cso.1. mouse.

Organism-specific databases

CTD3782.
MGIMGI:2153183. Kcnn3.

Phylogenomic databases

eggNOGNOG320393.
GeneTreeENSGT00500000044784.
HOVERGENHBG052241.
InParanoidP58391.
KOK04944.
OMAVYNALFY.
OrthoDBEOG73FQMC.
PhylomeDBP58391.
TreeFamTF315015.

Gene expression databases

GenevestigatorP58391.

Family and domain databases

InterProIPR013099. 2pore_dom_K_chnl_dom.
IPR004178. CaM-bd_dom.
IPR015449. K_chnl_Ca-activ_SK.
[Graphical view]
PANTHERPTHR10153. PTHR10153. 1 hit.
PfamPF02888. CaMBD. 1 hit.
PF07885. Ion_trans_2. 1 hit.
PF03530. SK_channel. 1 hit.
[Graphical view]
PRINTSPR01451. SKCHANNEL.
SMARTSM01053. CaMBD. 1 hit.
[Graphical view]
SUPFAMSSF81327. SSF81327. 1 hit.
ProtoNetSearch...

Other

NextBio369816.
PROP58391.
SOURCESearch...

Entry information

Entry nameKCNN3_MOUSE
AccessionPrimary (citable) accession number: P58391
Secondary accession number(s): Q3UUY9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: October 16, 2013
Last modified: July 9, 2014
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot