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P58389

- PTPA_MOUSE

UniProt

P58389 - PTPA_MOUSE

Protein

Serine/threonine-protein phosphatase 2A activator

Gene

Ppp2r4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (16 Nov 2001)
      Previous versions | rss
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    Functioni

    PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Acts as a regulatory subunit for serine/threonine-protein phosphatase 2A (PP2A) modulating its activity or substrate specificity, probably by inducing a conformational change in the catalytic subunit, a proposed direct target of the PPIase. Can reactivate inactive phosphatase PP2A-phosphatase methylesterase complexes (PP2A(i)) in presence of ATP and Mg2+. Reversibly stimulates the variable phosphotyrosyl phosphatase activity of PP2A core heterodimer PP2A(D) in presence of ATP and Mg2+ (in vitro). The phosphotyrosyl phosphatase activity is dependent of an ATPase activity of the PP2A(D):PPP2R4 complex. Is involved in apoptosis; the function appears to be independent from PP2A By similarity.By similarity

    Catalytic activityi

    Peptidylproline (omega=180) = peptidylproline (omega=0).

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi148 – 1547ATPBy similarity
    Nucleotide bindingi205 – 2073ATPBy similarity
    Nucleotide bindingi307 – 3082ATPBy similarity

    GO - Molecular functioni

    1. ATPase activity Source: Ensembl
    2. ATP binding Source: HGNC
    3. peptidyl-prolyl cis-trans isomerase activity Source: RefGenome
    4. protein homodimerization activity Source: HGNC
    5. protein phosphatase 2A binding Source: HGNC
    6. protein phosphatase type 2A regulator activity Source: HGNC
    7. protein tyrosine phosphatase activator activity Source: HGNC

    GO - Biological processi

    1. mitotic spindle organization in nucleus Source: RefGenome
    2. negative regulation of phosphoprotein phosphatase activity Source: HGNC
    3. negative regulation of protein dephosphorylation Source: HGNC
    4. positive regulation of apoptotic process Source: Ensembl
    5. positive regulation of phosphoprotein phosphatase activity Source: HGNC
    6. positive regulation of protein dephosphorylation Source: HGNC
    7. protein folding Source: UniProtKB-KW
    8. protein peptidyl-prolyl isomerization Source: GOC
    9. regulation of phosphoprotein phosphatase activity Source: HGNC

    Keywords - Molecular functioni

    Isomerase, Rotamase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase 2A activator (EC:5.2.1.8)
    Alternative name(s):
    PP2A, subunit B', PR53 isoform
    Phosphotyrosyl phosphatase activator
    Short name:
    PTPA
    Serine/threonine-protein phosphatase 2A regulatory subunit 4
    Serine/threonine-protein phosphatase 2A regulatory subunit B'
    Gene namesi
    Name:Ppp2r4
    Synonyms:Ptpa
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:1346006. Ppp2r4.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity

    GO - Cellular componenti

    1. calcium channel complex Source: Ensembl
    2. cytoplasm Source: RefGenome
    3. nucleus Source: RefGenome
    4. protein phosphatase type 2A complex Source: RefGenome

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 323322Serine/threonine-protein phosphatase 2A activatorPRO_0000071525Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP58389.
    PaxDbiP58389.
    PRIDEiP58389.

    PTM databases

    PhosphoSiteiP58389.

    Expressioni

    Gene expression databases

    ArrayExpressiP58389.
    BgeeiP58389.
    GenevestigatoriP58389.

    Interactioni

    Subunit structurei

    Associates with PP2A heterodimeric core enzyme PP2A(D), composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A) By similarity. Interacts with PPP2CB.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi225960. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP58389.
    SMRiP58389. Positions 23-322.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PTPA-type PPIase family.Curated

    Phylogenomic databases

    eggNOGiCOG5057.
    HOGENOMiHOG000205736.
    HOVERGENiHBG019168.
    InParanoidiP58389.
    KOiK17605.
    OMAiGSHGVWS.
    OrthoDBiEOG7KQ21W.
    PhylomeDBiP58389.
    TreeFamiTF105555.

    Family and domain databases

    InterProiIPR004327. Phstyr_phstse_ac.
    [Graphical view]
    PANTHERiPTHR10012. PTHR10012. 1 hit.
    PfamiPF03095. PTPA. 1 hit.
    [Graphical view]
    PIRSFiPIRSF016325. Phstyr_phstse_ac. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P58389-1 [UniParc]FASTAAdd to Basket

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    MAEGERQPPP DSSEETPPTT QNFIIPKKEI HTVPDMGKWK RSQAYADYIG    50
    FILTLNEGVK GKKLTFDYKV SEAIEKLVAL LDTLDRWIDE TPPVDQPSRF 100
    GNKAYRTWYA KLDQEAENLV ATVVPTHLAA AVPEVAVYLK EAVGNSTRID 150
    YGTGHEAAFA AFLCCLCKIG VLRVDDQVAI VFKVFDRYLE VMRKLQKTYR 200
    MEPAGSQGVW GLDDFQFLPF IWGSSQLIDH PHLEPRHFVD EKAVSENHKD 250
    YMFLQCILFI TEMKTGPFAE HSNQLWNISA VPSWSKVNQG LIRMYKAECL 300
    EKFPVIQHFK FGSLLPIHPV TSG 323
    Length:323
    Mass (Da):36,710
    Last modified:November 16, 2001 - v1
    Checksum:i1C7FA6C357A35F24
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti10 – 101Missing in AAK62028. 1 PublicationCurated
    Sequence conflicti196 – 1961Q → L in AAK62028. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY035997 mRNA. Translation: AAK62028.1.
    BC006626 mRNA. Translation: AAH06626.1.
    BC052852 mRNA. Translation: AAH52852.1.
    CCDSiCCDS15883.1.
    RefSeqiNP_620087.3. NM_138748.5.
    UniGeneiMm.275393.

    Genome annotation databases

    EnsembliENSMUST00000042055; ENSMUSP00000046837; ENSMUSG00000039515.
    GeneIDi110854.
    KEGGimmu:110854.
    UCSCiuc008jcn.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY035997 mRNA. Translation: AAK62028.1 .
    BC006626 mRNA. Translation: AAH06626.1 .
    BC052852 mRNA. Translation: AAH52852.1 .
    CCDSi CCDS15883.1.
    RefSeqi NP_620087.3. NM_138748.5.
    UniGenei Mm.275393.

    3D structure databases

    ProteinModelPortali P58389.
    SMRi P58389. Positions 23-322.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 225960. 1 interaction.

    PTM databases

    PhosphoSitei P58389.

    Proteomic databases

    MaxQBi P58389.
    PaxDbi P58389.
    PRIDEi P58389.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000042055 ; ENSMUSP00000046837 ; ENSMUSG00000039515 .
    GeneIDi 110854.
    KEGGi mmu:110854.
    UCSCi uc008jcn.2. mouse.

    Organism-specific databases

    CTDi 5524.
    MGIi MGI:1346006. Ppp2r4.

    Phylogenomic databases

    eggNOGi COG5057.
    HOGENOMi HOG000205736.
    HOVERGENi HBG019168.
    InParanoidi P58389.
    KOi K17605.
    OMAi GSHGVWS.
    OrthoDBi EOG7KQ21W.
    PhylomeDBi P58389.
    TreeFami TF105555.

    Miscellaneous databases

    NextBioi 364789.
    PROi P58389.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P58389.
    Bgeei P58389.
    Genevestigatori P58389.

    Family and domain databases

    InterProi IPR004327. Phstyr_phstse_ac.
    [Graphical view ]
    PANTHERi PTHR10012. PTHR10012. 1 hit.
    Pfami PF03095. PTPA. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF016325. Phstyr_phstse_ac. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the mouse homolog of the human phosphotyrosyl phosphatase activator, PTPA."
      David J., Yang X., Hashimoto K., Ramotar D.
      Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C3H/He and FVB/N.
      Tissue: Mammary gland and Osteoblast.
    3. "A novel and essential mechanism determining specificity and activity of protein phosphatase 2A (PP2A) in vivo."
      Fellner T., Lackner D.H., Hombauer H., Piribauer P., Mudrak I., Zaragoza K., Juno C., Ogris E.
      Genes Dev. 17:2138-2150(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PPP2CB.

    Entry informationi

    Entry nameiPTPA_MOUSE
    AccessioniPrimary (citable) accession number: P58389
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 16, 2001
    Last sequence update: November 16, 2001
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3