P58382 (ACKA_RHIME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetate kinase
EC=2.7.2.1

Alternative name(s):
Acetokinase

Gene names

Name:	ackA
Ordered Locus Names:	RB0850
ORF Names:	SMb21184

Encoded on

Plasmid pSymB (megaplasmid 2)

Organism

Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

266834 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + acetate = ADP + acetyl phosphate. HAMAP MF_00020 ATP + propanoate = ADP + propanoyl phosphate. HAMAP MF_00020
Pathway	Metabolic intermediate biosynthesis; acetyl-CoA biosynthesis; acetyl-CoA from acetate: step 1/2. HAMAP MF_00020
Subcellular location	Cytoplasm By similarity HAMAP MF_00020.
Sequence similarities	Belongs to the acetokinase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	organic acid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 392	392	Acetate kinase HAMAP MF_00020		PRO_0000107603

Sequences

	Sequence		Length	Mass (Da)
	P58382 [UniParc]. Last modified November 16, 2001. Version 1. Checksum: 5D7813B36567BD32	FASTA	392	42,023
	10 20 30 40 50 60 MEALLVINAG SSSLKFQIFG IATAGLERQV RAKLDGIATQ PRLKATAADG TELINRRLDA 70 80 90 100 110 120 TAVPDLPEAL SVARDWLATL RGFDLRAIGH RVVHGGPDYV RPVLIETTVL DRLASYQDLA 130 140 150 160 170 180 PLHQPNNLAP IRLAMDIKPD VPQVACFDTA FHRGRTEHTD CYALPRTFYE QGVRRYGFHG 190 200 210 220 230 240 ISYEYIAGRL REVAPEVARG RVIVAHLGSG ASMCALKDGR SVETTMGFTA LDGLPMGTRP 250 260 270 280 290 300 GQLDPGVVLH LLTDQGMSAQ AVSDLLYHRS GLKGLSGISN DMRELLGSDD PRASFAIDHF 310 320 330 340 350 360 VHRCALDAGM LAAALGGLDA FVFTAGIGEN SAPIRARVSE GLAWLGAELD PAANDAGASV 370 380 390 ISKAGSRVAL HVMPTDEELM IARHTLAIIR AR

References

[1]	"The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing endosymbiont Sinorhizobium meliloti." Finan T.M., Weidner S., Wong K., Buhrmester J., Chain P., Vorhoelter F.J., Hernandez-Lucas I., Becker A., Cowie A., Gouzy J., Golding B., Puehler A. Proc. Natl. Acad. Sci. U.S.A. 98:9889-9894(2001) [PubMed: 11481431] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.
[2]	"The composite genome of the legume symbiont Sinorhizobium meliloti." Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F., Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G., Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P., Cowie A. , Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S., Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I., Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S., Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C., Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R., Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H., Wong K., Yeh K.-C., Batut J. Science 293:668-672(2001) [PubMed: 11474104] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL591985 Genomic DNA. Translation: CAC49250.1.
PIR	B95948.
RefSeq	NP_437390.1. NC_003078.1.
3D structure databases
ProteinModelPortal	P58382.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1237181.
GenomeReviews	Gene locus RB0850 in contig AL591985_GR.
KEGG	sme:SM_b21184.
NMPDR	fig|266834.1.peg.5485.
PATRIC	23638120. VBISinMel96828_5781.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285863.
OMA	EAIDYFV.
ProtClustDB	PRK00180.
Enzyme and pathway databases
BioCyc	SMEL266834:SMB21184-MONOMER.
Family and domain databases
HAMAP	MF_00020. Acetate_kinase. [Tree]
InterPro	IPR004372. Ac/Proprionate_kinase. IPR000890. Aliphatic_acid_kin_short-chain. IPR023865. Aliphatic_acid_kinase_CS. [Graphical view]
KO	K00925.
PANTHER	PTHR21060. Acetate_kin. 1 hit.
Pfam	PF00871. Acetate_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000722. Acetate_prop_kin. 1 hit.
PRINTS	PR00471. ACETATEKNASE.
TIGRFAMs	TIGR00016. AckA. 1 hit.
PROSITE	PS01075. ACETATE_KINASE_1. 1 hit. PS01076. ACETATE_KINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACKA_RHIME

Accession

Primary (citable) accession number: P58382

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	November 16, 2001
Last modified:	January 25, 2012
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program