Reviewed, UniProtKB/Swiss-Prot P58381 (FABI2_RHIME)

Names and origin

Protein names

Recommended name:
    Enoyl-[acyl-carrier-protein] reductase [NADH] 2
    EC=1.3.1.9
Alternative name(s):
    NADH-dependent enoyl-ACP reductase 2

Gene names

Name:	fabI2
Ordered Locus Names:	R00246
ORF Names:	SMc00326

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acyl-[acyl-carrier-protein] + NAD+ = trans-2,3-dehydroacyl-[acyl-carrier-protein] + NADH.
Pathway	Lipid metabolism; fatty acid biosynthesis.
Subcellular location	Cell inner membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	binding Inferred from electronic annotation. Source: InterPro enoyl-[acyl-carrier-protein] reductase (NADH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 268	268	Enoyl-[acyl-carrier-protein] reductase [NADH] 2		PRO_0000054906
Regions
	Nucleotide binding	11 – 37	27	NAD By similarity
Sites
	Active site	156	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P58381-1 [UniParc]. Last modified November 16, 2001. Version 1. Checksum: 83D2127C7D76628C	FASTA	268	28,672
	10 20 30 40 50 60 MNGLMNGKRG LIMGVANSHS IAWGIAKSLA AQGAELAFTY QGEALGKRVK PLAAEVNSDF 70 80 90 100 110 120 LLPCDVEDIG SVDAVVDAIK ERWGKLDFVV HAIGFSDKNE LKGLYADTTR DNFSRTMVIS 130 140 150 160 170 180 CFSFTEIAKR AAELMSEGGT MLTLTYGGSM RVMPNYNVMG VAKAALEASV RYLAADYGSR 190 200 210 220 230 240 GIRVNAISAG PIRTLAGAGI SDARAMLSWQ QKNSPLRRTV TIEDVGSSAL YLLSDLSRGV 250 260 TGEIHYVDSG YNITSMPTLE ALRVADAD

References

[1]

"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed: 11481430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL591688 Genomic DNA. Translation: CAC41683.1.
RefSeq	NP_384352.1.
3D structure databases
HSSP	HSSP built from PDB template 1I30 based on UniProtKB P29132.
ModBase	Search...
Genome annotation databases
GeneID	1231878.
GenomeReviews	Gene locus R00246 in contig AL591688_GR.
KEGG	sme:SMc00326.
NMPDR	fig|266834.1.peg.1540.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P58381.
OMA	P58381. ADTTREN.
Enzyme and pathway databases
BioCyc	SMEL266834:SMC00326-MON.
BRENDA	1.3.1.9. 142.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR014358. Enoyl-ACP_Rdtase_NADH. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit. PTHR19410:SF12. Enoyl-ACP_rdct. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH.
ProtoNet	Search...

Entry information

Entry name

FABI2_RHIME

Accession

Primary (citable) accession number: P58381

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	November 16, 2001
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)