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Protein

Enoyl-[acyl-carrier-protein] reductase [NADH] 2

Gene

fabI2

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

Pathway: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei14 – 141NAD; via carbonyl oxygenBy similarity
Binding sitei41 – 411NADBy similarity
Binding sitei93 – 931NAD; via carbonyl oxygenBy similarity
Active sitei146 – 1461Proton acceptorBy similarity
Active sitei156 – 1561Proton acceptorBy similarity
Binding sitei163 – 1631NADBy similarity
Sitei204 – 2041Involved in acyl-ACP bindingBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi20 – 212NADBy similarity
Nucleotide bindingi65 – 662NADBy similarity
Nucleotide bindingi192 – 1965NADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciSMEL266834:GJF6-255-MONOMER.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH] 2 (EC:1.3.1.9)
Alternative name(s):
NADH-dependent enoyl-ACP reductase 2
Gene namesi
Name:fabI2
Ordered Locus Names:R00246
ORF Names:SMc00326
OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifieri266834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
ProteomesiUP000001976 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 268268Enoyl-[acyl-carrier-protein] reductase [NADH] 2PRO_0000054906Add
BLAST

Proteomic databases

PRIDEiP58381.

Interactioni

Protein-protein interaction databases

STRINGi266834.SMc00326.

Structurei

3D structure databases

ProteinModelPortaliP58381.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0623.
KOiK00208.
OMAiGKKWDGL.
OrthoDBiEOG6HF644.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.
PRINTSiPR00081. GDHRDH.

Sequencei

Sequence statusi: Complete.

P58381-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNGLMNGKRG LIMGVANSHS IAWGIAKSLA AQGAELAFTY QGEALGKRVK
60 70 80 90 100
PLAAEVNSDF LLPCDVEDIG SVDAVVDAIK ERWGKLDFVV HAIGFSDKNE
110 120 130 140 150
LKGLYADTTR DNFSRTMVIS CFSFTEIAKR AAELMSEGGT MLTLTYGGSM
160 170 180 190 200
RVMPNYNVMG VAKAALEASV RYLAADYGSR GIRVNAISAG PIRTLAGAGI
210 220 230 240 250
SDARAMLSWQ QKNSPLRRTV TIEDVGSSAL YLLSDLSRGV TGEIHYVDSG
260
YNITSMPTLE ALRVADAD
Length:268
Mass (Da):28,672
Last modified:November 16, 2001 - v1
Checksum:i83D2127C7D76628C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591688 Genomic DNA. Translation: CAC41683.1.
RefSeqiNP_384352.1. NC_003047.1.
WP_003534375.1. NC_003047.1.

Genome annotation databases

EnsemblBacteriaiCAC41683; CAC41683; SMc00326.
GeneIDi1231878.
KEGGisme:SMc00326.
PATRICi23629683. VBISinMel96828_1613.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591688 Genomic DNA. Translation: CAC41683.1.
RefSeqiNP_384352.1. NC_003047.1.
WP_003534375.1. NC_003047.1.

3D structure databases

ProteinModelPortaliP58381.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi266834.SMc00326.

Proteomic databases

PRIDEiP58381.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC41683; CAC41683; SMc00326.
GeneIDi1231878.
KEGGisme:SMc00326.
PATRICi23629683. VBISinMel96828_1613.

Phylogenomic databases

eggNOGiCOG0623.
KOiK00208.
OMAiGKKWDGL.
OrthoDBiEOG6HF644.

Enzyme and pathway databases

UniPathwayiUPA00094.
BioCyciSMEL266834:GJF6-255-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.
PRINTSiPR00081. GDHRDH.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1021.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1021.

Entry informationi

Entry nameiFABI2_RHIME
AccessioniPrimary (citable) accession number: P58381
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: November 16, 2001
Last modified: May 27, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.