Reviewed, UniProtKB/Swiss-Prot P58380 (FABI1_RHIME)

Names and origin

Protein names

Recommended name:
    Enoyl-[acyl-carrier-protein] reductase [NADH] 1
    EC=1.3.1.9
Alternative name(s):
    NADH-dependent enoyl-ACP reductase 1

Gene names

Name:	fabI1
Ordered Locus Names:	R00898
ORF Names:	SMc00005

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acyl-[acyl-carrier-protein] + NAD+ = trans-2,3-dehydroacyl-[acyl-carrier-protein] + NADH.
Pathway	Lipid metabolism; fatty acid biosynthesis.
Subcellular location	Cell inner membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	binding Inferred from electronic annotation. Source: InterPro enoyl-[acyl-carrier-protein] reductase (NADH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 272	272	Enoyl-[acyl-carrier-protein] reductase [NADH] 1		PRO_0000054905
Regions
	Nucleotide binding	14 – 40	27	NAD By similarity
Sites
	Active site	159	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P58380-1 [UniParc]. Last modified November 16, 2001. Version 1. Checksum: 1E0FA18A22CDBE36	FASTA	272	29,148
	10 20 30 40 50 60 MAQASGLMNG KRGVIMGVAN NRSIAWGIAK ALAEAGAEIA LTWQGDALKK RVEPLAQELG 70 80 90 100 110 120 AFMAGHCDVT DLATIDAVFS ALEEKWGKID FVVHAIAFSD KDELTGRYLD TSRDNFARTM 130 140 150 160 170 180 DISVYSFTAV AARADRVMND GGSILTLTYY GAEKVMPHYN VMGVAKAALE ASVRYLAVDL 190 200 210 220 230 240 GNRGIRVNAI SAGPIKTLAA SGIGDFRYIL KWNEYNAPLK RTVSIEEVGN SALYLLSDLS 250 260 270 SGVTGEVHHV DSGYHTVGMK AVDAPDISVL KD

References

[1]

"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed: 11481430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL591688 Genomic DNA. Translation: CAC45470.1.
RefSeq	NP_385004.1.
3D structure databases
HSSP	HSSP built from PDB template 1I30 based on UniProtKB P29132.
ModBase	Search...
Genome annotation databases
GeneID	1232539.
GenomeReviews	Gene locus R00898 in contig AL591688_GR.
KEGG	sme:SMc00005.
NMPDR	fig|266834.1.peg.2192.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P58380.
OMA	P58380. LVHCLAF.
Enzyme and pathway databases
BioCyc	SMEL266834:SMC00005-MON.
BRENDA	1.3.1.9. 142.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR014358. Enoyl-ACP_Rdtase_NADH. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit. PTHR19410:SF12. Enoyl-ACP_rdct. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH.
ProtoNet	Search...

Entry information

Entry name

FABI1_RHIME

Accession

Primary (citable) accession number: P58380

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	November 16, 2001
Last modified:	June 16, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)