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Reviewed, UniProtKB/Swiss-Prot P58363 (ARCB_ECO57)

Last modified November 3, 2009. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Aerobic respiration control sensor protein arcB
    EC=2.7.13.3
Gene names
Name: arcB
Ordered Locus Names: Z4574, ECs4089
OrganismEscherichia coli O157:H7 [Complete proteome] [HAMAP]
Taxonomic identifier83334 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length778 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Member of the two-component regulatory system arcB/arcA. Sensor-regulator protein for anaerobic repression of the arc modulon. Activates arcA via a four-step phosphorelay. ArcB can also dephosphorylate arcA by a reverse phosphorelay involving His-717 and Asp-576 By similarity.

Catalytic activity

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Subcellular location

Cell inner membrane; Multi-pass membrane protein By similarity.

Post-translational modification

Activation requires a sequential transfer of a phosphate group from a His in the primary transmitter domain, to an Asp in the receiver domain and to a His in the secondary transmitter domain By similarity.

Sequence similarities

Contains 1 histidine kinase domain.

Contains 1 HPt domain.

Contains 1 PAC (PAS-associated C-terminal) domain.

Contains 1 PAS (PER-ARNT-SIM) domain.

Contains 1 response regulatory domain.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 778778Aerobic respiration control sensor protein arcB
PRO_0000074685

Regions

Topological domain1 – 2525Cytoplasmic Potential
Transmembrane26 – 4621 Potential
Topological domain47 – 5711Periplasmic Potential
Transmembrane58 – 7821 Potential
Topological domain79 – 778700Cytoplasmic Potential
Domain153 – 22371PAS
Domain226 – 27853PAC
Domain289 – 507219Histidine kinase
Domain527 – 643117Response regulatory
Domain678 – 77194HPt

Amino acid modifications

Modified residue2921Phosphohistidine; by autocatalysis By similarity
Modified residue57614-aspartylphosphate By similarity
Modified residue7171Phosphohistidine By similarity

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Sequences

Sequence LengthMass (Da)Tools
P58363-1 [UniParc].

Last modified November 16, 2001. Version 1.
Checksum: C8AE004B007F9D30

FASTA77888,010
        10         20         30         40         50         60 
MKQIRLLAQY YVDLMMKLGL VRFSMLLALA LVVLAIVVQM AVTMVLHGQV ESIDVIRSIF 

        70         80         90        100        110        120 
FGLLITPWAV YFLSVVVEQL EESRQRLSRL VQKLEEMRER DLSLNVQLKD NIAQLNQEIA 

       130        140        150        160        170        180 
VREKAEAELQ ETFGQLKIEI KEREETQIQL EQQSSFLRSF LDASPDLVFY RNEDKEFSGC 

       190        200        210        220        230        240 
NRAMELLTGK SEKQLVHLKP ADVYSPEAAA KVIETDEKVF RHNVSLTYEQ WLDYPDGRKA 

       250        260        270        280        290        300 
CFEIRKVPYY DRVGKRHGLM GFGRDITERK RYQDALERAS RDKTTFISTI SHELRTPLNG 

       310        320        330        340        350        360 
IVGLSRILLD TELTAEQEKY LKTIHVSAVT LGNIFNDIID MDKMERRKVQ LDNQPVDFTS 

       370        380        390        400        410        420 
FLADLENLSA LQAQQKGLRF NLEPTLPLPH QVITDGTRLR QILWNLISNA VKFTQQGQVT 

       430        440        450        460        470        480 
VRVRYDEGDM LHFEVEDSGI GIPQDELDKI FAMYYQVKDS HGGKPATGTG IGLAVSRRLA 

       490        500        510        520        530        540 
KNMGGDITVT SEQGKGSTFT LTIHAPSVAE EVDDAFDEDD MPLPALNVLL VEDIELNVIV 

       550        560        570        580        590        600 
ARSVLEKLGN SVDVAMTGKA ALEMFKPGEY DLVLLDIQLP DMTGLDISRE LTKRYPREDL 

       610        620        630        640        650        660 
PPLVALTANV LKDKQEYLNA GMDDVLSKPL SVPALTAMIK KFWDTQDDEE STVTTEENSK 

       670        680        690        700        710        720 
SEALLDIPML EQYLELVGPK LITDGLAVFE KMMPGYVNVL ESNLTAQDKK GIVEEGHKIK 

       730        740        750        760        770 
GAAGSVGLRH LQQLGQQIQS PDLPAWEDNV GEWIEEMKEE WRHDVEVLKA WVAKATKK

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References

[1]"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7."
Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. expand/collapse author list , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R.
Nature 409:529-533(2001) [PubMed: 11206551] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
[2]"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. expand/collapse author list , Kuhara S., Shiba T., Hattori M., Shinagawa H.
DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

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Cross-references

Sequence databases

AE005174 Genomic DNA. Translation: AAG58344.1.
BA000007 Genomic DNA. Translation: BAB37512.1.
PIRA91140.
D85985.
RefSeqNP_289784.1.
NP_312116.1.

3D structure databases

HSSPHSSP built from PDB template 1FR0 based on UniProtKB P22763.
SMRP58363. Positions 659-776.
ModBaseSearch...

Genome annotation databases

GeneID916062.
958650.
GenomeReviewsGene locus Z4574 in contig AE005174_GR.
Gene locus ECs4089 in contig BA000007_GR.
KEGGece:Z4574.
ecs:ECs4089.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMP58363.
OMAVVEWIEE.

Enzyme and pathway databases

BioCycECOL83334:ECS4089-MON.
BRENDA2.7.13.3. 246.

Family and domain databases

InterProIPR003594. ATP_bd_ATPase.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR013767. PAS_fold.
IPR004358. Sig_transdc_His_kin-like_C.
IPR014409. Sig_transdc_His_kin_hyb_ArcB.
IPR008207. Sig_transdc_His_kin_P-trf.
IPR003661. Sig_transdc_His_kin_sub1_dim/P.
IPR005467. Sig_transdc_His_kinase_core.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
Gene3DG3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
PfamPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF01627. Hpt. 1 hit.
PF00989. PAS. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PIRSFPIRSF003182. ArcB. 1 hit.
PRINTSPR00344. BCTRLSENSOR.
ProDomPD000039. Response_reg. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00073. HPT. 1 hit.
SM00091. PAS. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
TIGRFAMsTIGR00229. sensory_box. 1 hit.
PROSITEPS50109. HIS_KIN. 1 hit.
PS50894. HPT. 1 hit.
PS50113. PAC. 1 hit.
PS50112. PAS. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameARCB_ECO57
AccessionPrimary (citable) accession number: P58363
Entry history
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: November 16, 2001
Last modified: November 3, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents