Reviewed, UniProtKB/Swiss-Prot P58350 (AATB1_RHIME)

Names and origin

Protein names

Recommended name:
    Aspartate aminotransferase B
      Short name=AspAT
    EC=2.6.1.1
Alternative name(s):
    Transaminase A

Gene names

Name:	aatB
Ordered Locus Names:	R03291
ORF Names:	SMc04386

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	410 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in symbiotic nitrogen fixation By similarity.
Catalytic activity	L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.
Cofactor	Pyridoxal phosphate By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	1-aminocyclopropane-1-carboxylate synthase activity Inferred from electronic annotation. Source: InterPro L-aspartate:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 410	410	Aspartate aminotransferase B		PRO_0000123848
Amino acid modifications
	Modified residue	249	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P58350-1 [UniParc]. Last modified November 16, 2001. Version 1. Checksum: DC720126C0C654DB	FASTA	410	44,375
	10 20 30 40 50 60 MTINATVKEA GFQPASRISS IGVSEILKIG ARAAAMKREG KPVIILGAGE PDFDTPEHVK 70 80 90 100 110 120 QAASDAIHRG ETKYTALDGT PELKKAIREK FQRENGLAYE LDEITVATGA KQILFNAMMA 130 140 150 160 170 180 SLDPGDEVII PTPYWTSYSD IVHICEGKPV LIACDASSGF RLTAEKLEAA ITPRTRWVLL 190 200 210 220 230 240 NSPSNPSGAA YSAADYRPLL EVLLRHPHVW LLVDDMYEHI VYDGFRFVTP AQLEPGLKNR 250 260 270 280 290 300 TLTVNGVSKA YAMTGWRIGY AGGPRELIKA MAVVQSQATS CPSSISQAAS VAALNGPQDF 310 320 330 340 350 360 LKERTESFQR RRDLVVNGLN AIDGLDCRVP EGAFYTFSGC AGVLGKVTPS GKRIKTDTDF 370 380 390 400 410 CAYLLEDAHV AVVPGSAFGL SPFFRISYAT SEAELKEALE RIAAACDRLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sinorhizobium meliloti aatB gene region." Watson R.J., Heys R. Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: JJ1c10.
[2]	"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed: 11481430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF448466 Genomic DNA. Translation: AAL41013.1. AL591688 Genomic DNA. Translation: CAC47870.1.
RefSeq	NP_387397.1.
3D structure databases
HSSP	HSSP built from PDB template 1BKG based on UniProtKB Q56232.
ModBase	Search...
Genome annotation databases
GeneID	1234985.
GenomeReviews	Gene locus R03291 in contig AL591688_GR.
KEGG	sme:SMc04386.
NMPDR	fig|266834.1.peg.4585.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P58350.
OMA	KERTESF.
Enzyme and pathway databases
BioCyc	SMEL266834:SMC04386-MON.
BRENDA	2.6.1.1. 142.
Family and domain databases
InterPro	IPR001176. ACC_synthase. IPR004839. Aminotransferase_I/II. IPR004838. NHTrfase_class1_PyrdxlP-BS. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
PRINTS	PR00753. ACCSYNTHASE.
PROSITE	PS00105. AA_TRANSFER_CLASS_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AATB1_RHIME

Accession

Primary (citable) accession number: P58350

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	November 16, 2001
Last modified:	November 3, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)