P58349 (RBS_RHIME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribulose bisphosphate carboxylase small chain
Short name=RuBisCO small subunit
EC=4.1.1.39

Gene names

Name:	cbbS
Ordered Locus Names:	RB0190
ORF Names:	SMb20197

Encoded on

Plasmid pSymB (megaplasmid 2)

Organism

Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

266834 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium › Rhizobium meliloti

Protein attributes

Sequence length	129 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site By similarity.
Catalytic activity	2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O. 3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.
Subunit structure	8 large chains + 8 small chains.
Sequence similarities	Belongs to the RuBisCO small chain family.

Ontologies

Keywords
Biological process	Calvin cycle Carbon dioxide fixation
Molecular function	Lyase Monooxygenase Oxidoreductase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological_process	reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW ribulose-bisphosphate carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 129	129	Ribulose bisphosphate carboxylase small chain		PRO_0000198619

Sequences

	Sequence		Length	Mass (Da)
	P58349 [UniParc]. Last modified November 16, 2001. Version 1. Checksum: B3A4DF100179F3FE	FASTA	129	15,147
	10 20 30 40 50 60 MRITQGCFSF LPDLTDEQIT AQVQYCLGKG WAIGVEYTDD PHPRNTYWEM WGNPMFDLKD 70 80 90 100 110 120 AKGVMMELED CRKAHPQDYI RLNAFDSSRG LETVTMSFIV NRPENEPSLR MTRTESNGRS QHYMWETQR

References

[1]

"The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing endosymbiont Sinorhizobium meliloti." Finan T.M., Weidner S., Wong K., Buhrmester J., Chain P., Vorhoelter F.J., Hernandez-Lucas I., Becker A., Cowie A., Gouzy J., Golding B., Puehler A. Proc. Natl. Acad. Sci. U.S.A. 98:9889-9894(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

[2]

"The composite genome of the legume symbiont Sinorhizobium meliloti." Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F., Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G., Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P., Cowie A. , Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S., Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I., Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S., Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C., Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R., Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H., Wong K., Yeh K.-C., Batut J. Science 293:668-672(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL591985 Genomic DNA. Translation: CAC48590.1.
PIR	F95865.
RefSeq	NP_436730.1. NC_003078.1.
3D structure databases
ProteinModelPortal	P58349.
SMR	P58349. Positions 1-127.
ModBase	Search...
Protein-protein interaction databases
STRING	266834.SM_b20197.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAC48590; CAC48590; SM_b20197.
GeneID	1236521.
KEGG	sme:SM_b20197.
PATRIC	23636769. VBISinMel96828_5106.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG4451.
KO	K01602.
OMA	WAVNIEF.
ProtClustDB	CLSK892105.
Enzyme and pathway databases
BioCyc	SMEL266834:GJF6-3623-MONOMER.
Family and domain databases
Gene3D	3.30.190.10. 1 hit.
InterPro	IPR000894. RuBisCO_sc_dom. [Graphical view]
Pfam	PF00101. RuBisCO_small. 1 hit. [Graphical view]
SMART	SM00961. RuBisCO_small. 1 hit. [Graphical view]
SUPFAM	SSF55239. RuBisCO_small. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RBS_RHIME

Accession

Primary (citable) accession number: P58349

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	November 16, 2001
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program