Reviewed, UniProtKB/Swiss-Prot P58342 (ATCU2_RHIME)

Names and origin

Protein names

Recommended name:
    Copper-transporting ATPase 2
    EC=3.6.3.4

Gene names

Name:	actP2
Synonyms:	atcU2
Ordered Locus Names:	RB1018
ORF Names:	SMb21578

Encoded on

Plasmid pSymB (megaplasmid 2)

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	827 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in copper transport By similarity.
Catalytic activity	ATP + H(2)O + Cu(2+)(In) = ADP + phosphate + Cu(2+)(Out).
Subcellular location	Cell membrane; Multi-pass membrane proteinBy similarity.
Sequence similarities	Belongs to the cation transport ATPase (P-type) family. Type IB subfamily. Contains 2 HMA domains.

Ontologies

Keywords
Biological process	Copper transport Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Repeat Transmembrane
Ligand	ATP-binding Copper Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	copper ion transport Inferred from electronic annotation. Source: InterPro metabolic process Inferred from electronic annotation. Source: InterPro proton transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro copper ion binding Inferred from electronic annotation. Source: InterPro copper-exporting ATPase activity Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 827	827	Copper-transporting ATPase 2		PRO_0000046328
Regions
	Transmembrane	174 – 194	21	Potential
	Transmembrane	210 – 230	21	Potential
	Transmembrane	246 – 266	21	Potential
	Transmembrane	271 – 291	21	Potential
	Transmembrane	430 – 450	21	Potential
	Transmembrane	458 – 478	21	Potential
	Transmembrane	771 – 793	23	Potential
	Transmembrane	797 – 819	23	Potential
	Domain	16 – 81	66	HMA 1
	Domain	83 – 149	67	HMA 2
Sites
	Active site	515	1	4-aspartylphosphate intermediate By similarity
	Metal binding	26	1	Copper Potential
	Metal binding	29	1	Copper Potential
	Metal binding	93	1	Copper Potential
	Metal binding	96	1	Copper Potential
	Metal binding	714	1	Magnesium By similarity
	Metal binding	718	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	P58342-1 [UniParc]. Last modified November 2, 2001. Version 1. Checksum: A3D8DFFDD1315FCB	FASTA	827	85,862
	10 20 30 40 50 60 MTALKKIEKA APLPVSTNFG IEGMTCASCV RRVEKAITAV PGVASANVNL ATERATVQFD 70 80 90 100 110 120 GEPDTLAVLH AIEKAGYAPR IATEELQIEG MTCASCVSRV EKALKAVPGV ADAAVNLATE 130 140 150 160 170 180 KATVSLISGT ADLSALEAAV RGAGYELRKT KPAEASAGDE DHRAAELGSL KSAVTISVLM 190 200 210 220 230 240 TLPLFLMEMG SHFISGVHEL IMGTIGMRNN LYLQFALATL VLFGPGLRFF RKGVPNLLRW 250 260 270 280 290 300 TPDMNSLVVL GTTAAWGYSV VATFVPRVLP SGTANVYYEA AAVIVTLVLL GRYLESRAKG 310 320 330 340 350 360 RTSQAIKRLV GLQPKTAFVL RGGEFVEAQI SEVVAGDVIR IRPGEKIPVD GTVIDGSSYV 370 380 390 400 410 420 DEAMITGEPL PVQKTADSAV VGGTINKTGS ITFKATKVGS DTLLAQIIKL VETAQGSKLP 430 440 450 460 470 480 IQALVDRVTG WFVPAVILAA VLTFAAWYTF GPSPALSFAL VNAVAVLIIA CPCAMGLATP 490 500 510 520 530 540 TSIMVGTGRA AELGILFRKG EALQRLRDAD VVALDKTGTL TKGRPELTDL VAAEGFEADE 550 560 570 580 590 600 VLFLVASLET LSEHPIAEAI VSAAKSKGIA TAAVNGFEAT PGFGVSGSVS GRQVLVGADR 610 620 630 640 650 660 ALATNGIDVS GFSTEAELLG ASGKSPLYAA IEGRLAAIVA VSDPVKETTP QAIRSLHELG 670 680 690 700 710 720 LKVAMITGDN RRTAEAIARK LGIDEVVAEV LPEGKVEAIR KLRQGGRSVA FIGDGINDAP 730 740 750 760 770 780 ALAEADVGIA VGTGTDIAIE SADVVLMSGD LNGVAKAIAL SKATILNIKQ NLFWAFAYNV 790 800 810 820 SLIPVAAGVL YPVTGILLSP IFAAAAMAMS SVFVLGNALR LKSVNPA

References

[1]

"The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing endosymbiont Sinorhizobium meliloti." Finan T.M., Weidner S., Wong K., Buhrmester J., Chain P., Vorhoelter F.J., Hernandez-Lucas I., Becker A., Cowie A., Gouzy J., Golding B., Puehler A. Proc. Natl. Acad. Sci. U.S.A. 98:9889-9894(2001) [PubMed: 11481431] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL591985 Genomic DNA. Translation: CAC49418.1.
PIR	B95969.
RefSeq	NP_437558.1.
3D structure databases
HSSP	HSSP built from PDB template 1KQK based on UniProtKB O32220.
ModBase	Search...
Genome annotation databases
GeneID	1237349.
GenomeReviews	Gene locus RB1018 in contig AL591985_GR.
KEGG	sme:SMb21578.
NMPDR	fig|266834.1.peg.5653.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P58342.
Enzyme and pathway databases
BioCyc	SMEL266834:SMB21578-MON.
Family and domain databases
InterPro	IPR006416. ATPase-IB_hvy. IPR001757. ATPase_P. IPR006403. ATPase_P_cat/Cu. IPR006122. Cu_ion_bd. IPR005834. Dehalogen-like_hydro. IPR008250. E1-E2_ATPase_reg. IPR000695. H_ATPase. IPR006121. HeavyMe_transpt. [Graphical view]
PANTHER	PTHR11939. ATPase_P. 1 hit.
Pfam	PF00122. E1-E2_ATPase. 1 hit. PF00403. HMA. 2 hits. PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE. PR00120. HATPASE.
TIGRFAMs	TIGR01511. ATPase-IB1_Cu. 1 hit. TIGR01525. ATPase-IB_hvy. 1 hit. TIGR01494. ATPase_P-type. 2 hits. TIGR00003. Cu_ion_bd. 2 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. PS01047. HMA_1. 2 hits. PS50846. HMA_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATCU2_RHIME

Accession

Primary (citable) accession number: P58342

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 2, 2001
Last sequence update:	November 2, 2001
Last modified:	November 25, 2008
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)