Reviewed, UniProtKB/Swiss-Prot P58341 (ATCU1_RHIME)

Names and origin

Protein names

Recommended name:
    Copper-transporting ATPase 1
    EC=3.6.3.4

Gene names

Name:	actP1
Synonyms:	actP
Ordered Locus Names:	RA0548
ORF Names:	SMa1013

Encoded on

Plasmid pSymA (megaplasmid 1)

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	826 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in copper transport By similarity.
Catalytic activity	ATP + H(2)O + Cu(2+)(In) = ADP + phosphate + Cu(2+)(Out).
Subcellular location	Cell membrane; Multi-pass membrane proteinBy similarity.
Sequence similarities	Belongs to the cation transport ATPase (P-type) family. Type IB subfamily. Contains 2 HMA domains.

Ontologies

Keywords
Biological process	Copper transport Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Repeat Transmembrane
Ligand	ATP-binding Copper Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	copper ion transport Inferred from electronic annotation. Source: InterPro metabolic process Inferred from electronic annotation. Source: InterPro proton transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro copper ion binding Inferred from electronic annotation. Source: InterPro copper-exporting ATPase activity Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 826	826	Copper-transporting ATPase 1		PRO_0000046327
Regions
	Transmembrane	172 – 192	21	Potential
	Transmembrane	209 – 229	21	Potential
	Transmembrane	246 – 266	21	Potential
	Transmembrane	270 – 290	21	Potential
	Transmembrane	429 – 449	21	Potential
	Transmembrane	457 – 477	21	Potential
	Transmembrane	772 – 792	21	Potential
	Transmembrane	795 – 815	21	Potential
	Domain	16 – 81	66	HMA 1
	Domain	83 – 148	66	HMA 2
Sites
	Active site	514	1	4-aspartylphosphate intermediate By similarity
	Metal binding	26	1	Copper Potential
	Metal binding	29	1	Copper Potential
	Metal binding	93	1	Copper Potential
	Metal binding	96	1	Copper Potential
	Metal binding	713	1	Magnesium By similarity
	Metal binding	717	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	P58341-1 [UniParc]. Last modified November 2, 2001. Version 1. Checksum: 91966D720D870F70	FASTA	826	86,285
	10 20 30 40 50 60 MTAFTQIEKS AAVPAPTDFG IEGMTCASCV RRVEKAISAV PGVASATVNL ATERASVQFT 70 80 90 100 110 120 GAPDTGGVLL AIEKAGYEPK VIIQEFGIEG MTCASCVSRV EKALRTVPGV ADASVNLATE 130 140 150 160 170 180 KGTVRFVSGV DVAAIEAAVR DAGYDVRKAK ASGATAEPED RRELETRTLK RLVILSAVLT 190 200 210 220 230 240 LPLFLVEMGS HFMPGVHEWI MENIGMRHNL YIQFALATAV LFGPGLRFFR KGVPNLLRWT 250 260 270 280 290 300 PDMNSLVVLG TTAAWGYSVV ATFASGLLPS GTANVYYEAA AVIVTLILLG RYLEARAKGR 310 320 330 340 350 360 TSQAIKRLLG LQPKTAFVAH GDEFVEIQIS DVVVGDVIRI RPGEKIPVDG TVLDGNSYVD 370 380 390 400 410 420 ESMITGEPVP VQKAAGAEVV GGTINKNGSF TFRATKVGGD TLLAQIIKMV ETAQGSKLPI 430 440 450 460 470 480 QALVDKVTAW FVPAVILVAV LTFAAWYVFG PSPALTFALV NAVAVLIIAC PCAMGLATPT 490 500 510 520 530 540 SIMVGTGRAA ELGILFRKGE ALQSLREADV IALDKTGTLT KGRPELTDIV PADGFEADEV 550 560 570 580 590 600 LSFVASLEAL SEHPIAEAIV SAAKSRGIAL VPATDFEATP GFGVRGAVSG LPVQVGADRA 610 620 630 640 650 660 FSGVGIDVSP FVVEAERLGN SGKSPLYAAI DGRLAAIIAV SDPIKDTTPQ AIKALHDLGL 670 680 690 700 710 720 KVAMITGDNR RTADAIARQL GIDEVVAEVL PDGKVDAVKR LREGGRKVAF IGDGINDAPA 730 740 750 760 770 780 LTEADVGIAV GTGTDIAIES ADVVLMSGDL IGVPKAIALS KATIRNIKQN LFWAFAYNVS 790 800 810 820 LVPVAAGVLY PLNGTLLSPI LAAAAMAMSS VFVLGNALRL RSVNPA

References

[1]

"Nucleotide sequence and predicted functions of the entire Sinorhizobium meliloti pSymA megaplasmid." Barnett M.J., Fisher R.F., Jones T., Komp C., Abola A.P., Barloy-Hubler F., Bowser L., Capela D., Galibert F., Gouzy J., Gurjal M., Hong A., Huizar L., Hyman R.W., Kahn D., Kahn M.L., Kalman S., Keating D.H. , Palm C., Peck M.C., Surzycki R., Wells D.H., Yeh K.-C., Davis R.W., Federspiel N.A., Long S.R. Proc. Natl. Acad. Sci. U.S.A. 98:9883-9888(2001) [PubMed: 11481432] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE006469 Genomic DNA. Translation: AAK65206.1.
PIR	D95330.
RefSeq	NP_435794.1.
3D structure databases
HSSP	HSSP built from PDB template 1JWW based on UniProtKB O32220.
ModBase	Search...
Genome annotation databases
GeneID	1235584.
GenomeReviews	Gene locus RA0548 in contig AE006469_GR.
KEGG	sme:SMa1013.
NMPDR	fig|266834.1.peg.548.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P58341.
Enzyme and pathway databases
BioCyc	SMEL266834:SMA1013-MON.
Family and domain databases
InterPro	IPR006416. ATPase-IB_hvy. IPR001757. ATPase_P. IPR006403. ATPase_P_cat/Cu. IPR006122. Cu_ion_bd. IPR005834. Dehalogen-like_hydro. IPR008250. E1-E2_ATPase_reg. IPR000695. H_ATPase. IPR006121. HeavyMe_transpt. [Graphical view]
PANTHER	PTHR11939. ATPase_P. 1 hit.
Pfam	PF00122. E1-E2_ATPase. 1 hit. PF00403. HMA. 2 hits. PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE. PR00120. HATPASE.
TIGRFAMs	TIGR01511. ATPase-IB1_Cu. 1 hit. TIGR01525. ATPase-IB_hvy. 1 hit. TIGR01494. ATPase_P-type. 2 hits. TIGR00003. Cu_ion_bd. 2 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. PS01047. HMA_1. 2 hits. PS50846. HMA_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATCU1_RHIME

Accession

Primary (citable) accession number: P58341

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 2, 2001
Last sequence update:	November 2, 2001
Last modified:	November 25, 2008
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)