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P58336

- ALF_RHIME

UniProt

P58336 - ALF_RHIME

Protein

Fructose-bisphosphate aldolase

Gene

cbbA

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (02 Nov 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.By similarity

    Catalytic activityi

    D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

    Cofactori

    Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei50 – 501Glyceraldehyde 3-phosphateBy similarity
    Active sitei83 – 831Proton donorBy similarity
    Metal bindingi84 – 841Zinc 1; catalyticBy similarity
    Metal bindingi105 – 1051Zinc 2By similarity
    Metal bindingi142 – 1421Zinc 2By similarity
    Metal bindingi198 – 1981Zinc 1; catalyticBy similarity
    Binding sitei199 – 1991Dihydroxyacetone phosphate; via amide nitrogenBy similarity
    Metal bindingi232 – 2321Zinc 1; catalyticBy similarity

    GO - Molecular functioni

    1. fructose-bisphosphate aldolase activity Source: UniProtKB-EC
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway
    2. reductive pentose-phosphate cycle Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Calvin cycle, Glycolysis

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciSMEL266834:GJF6-3625-MONOMER.
    UniPathwayiUPA00109; UER00183.
    UPA00116.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fructose-bisphosphate aldolase (EC:4.1.2.13)
    Short name:
    FBP aldolase
    Short name:
    FBPA
    Alternative name(s):
    Fructose-1,6-bisphosphate aldolase
    Gene namesi
    Name:cbbA
    Ordered Locus Names:RB0192
    ORF Names:SMb20199
    Encoded oniPlasmid pSymB (megaplasmid 2)0 Publication
    OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
    Taxonomic identifieri266834 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
    ProteomesiUP000001976: Plasmid pSymB

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 359359Fructose-bisphosphate aldolasePRO_0000178729Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi266834.SM_b20199.

    Structurei

    3D structure databases

    ProteinModelPortaliP58336.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni233 – 2353Dihydroxyacetone phosphate bindingBy similarity
    Regioni275 – 2784Dihydroxyacetone phosphate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0191.
    KOiK01624.
    OrthoDBiEOG6HXJ7B.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR006412. Fruct_bisP_Calv.
    IPR000771. Ketose_bisP_aldolase_II.
    [Graphical view]
    PfamiPF01116. F_bP_aldolase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
    TIGRFAMsiTIGR00167. cbbA. 1 hit.
    TIGR01521. FruBisAldo_II_B. 1 hit.
    PROSITEiPS00602. ALDOLASE_CLASS_II_1. 1 hit.
    PS00806. ALDOLASE_CLASS_II_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P58336-1 [UniParc]FASTAAdd to Basket

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    MARITLRQLL DHAAERSYGV PAFNINNMEQ GLAIMEAARA SDAPVILQVS    50
    RGARSYANDV MLAKMMEALE EMYPDIPLCI HQDHGNNVAT CLTAIQHGFT 100
    SVMMDGSLKE DAKTPADYDY NVSITAEVSR LAHMVGASVE GELGCLGSLE 150
    TGHGEAEDGH GFEGALDRSQ LLTDPDEAAR FVAETGVDAL AVAIGTSHGA 200
    YKFTRKPTGE VLAMDVIEKI HERLPDTHIV MHGSSSVPQE WQDVFNAHGG 250
    QMRETYGVPV EEIVRGIRFG VRKVNIDTDL RLAAAAAFRR VADTSRSEFD 300
    PRKFLKPAMD AMSAVCKARF EAFGTAGNAS RIKVVPMPEM ARRYASGSLK 350
    PQSARSEAA 359
    Length:359
    Mass (Da):38,909
    Last modified:November 2, 2001 - v1
    Checksum:i2589C30A0EC28DD3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL591985 Genomic DNA. Translation: CAC48592.1.
    PIRiH95865.
    RefSeqiNP_436732.1. NC_003078.1.

    Genome annotation databases

    EnsemblBacteriaiCAC48592; CAC48592; SM_b20199.
    GeneIDi1236523.
    KEGGisme:SM_b20199.
    PATRICi23636773. VBISinMel96828_5108.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL591985 Genomic DNA. Translation: CAC48592.1 .
    PIRi H95865.
    RefSeqi NP_436732.1. NC_003078.1.

    3D structure databases

    ProteinModelPortali P58336.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 266834.SM_b20199.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAC48592 ; CAC48592 ; SM_b20199 .
    GeneIDi 1236523.
    KEGGi sme:SM_b20199.
    PATRICi 23636773. VBISinMel96828_5108.

    Phylogenomic databases

    eggNOGi COG0191.
    KOi K01624.
    OrthoDBi EOG6HXJ7B.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00183 .
    UPA00116 .
    BioCyci SMEL266834:GJF6-3625-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR006412. Fruct_bisP_Calv.
    IPR000771. Ketose_bisP_aldolase_II.
    [Graphical view ]
    Pfami PF01116. F_bP_aldolase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001359. F_bP_aldolase_II. 1 hit.
    TIGRFAMsi TIGR00167. cbbA. 1 hit.
    TIGR01521. FruBisAldo_II_B. 1 hit.
    PROSITEi PS00602. ALDOLASE_CLASS_II_1. 1 hit.
    PS00806. ALDOLASE_CLASS_II_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing endosymbiont Sinorhizobium meliloti."
      Finan T.M., Weidner S., Wong K., Buhrmester J., Chain P., Vorhoelter F.J., Hernandez-Lucas I., Becker A., Cowie A., Gouzy J., Golding B., Puehler A.
      Proc. Natl. Acad. Sci. U.S.A. 98:9889-9894(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1021.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1021.

    Entry informationi

    Entry nameiALF_RHIME
    AccessioniPrimary (citable) accession number: P58336
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 2, 2001
    Last sequence update: November 2, 2001
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Plasmid, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3