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P58321 (UCHL4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase isozyme L4

Short name=UCH-L4
EC=3.4.19.12
Alternative name(s):
Ubiquitin thioesterase L4
Gene names
Name:Uchl4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length233 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Ubiquitin-protein hydrolase is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subcellular location

Cytoplasm By similarity.

Tissue specificity

Expressed in various tissues at low level.

Sequence similarities

Belongs to the peptidase C12 family.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Cellular componentCytoplasm
   Molecular functionHydrolase
Protease
Thiol protease
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processubiquitin-dependent protein catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionubiquitin-specific protease activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 233233Ubiquitin carboxyl-terminal hydrolase isozyme L4
PRO_0000211064

Regions

Region8 – 136Interaction with ubiquitin By similarity
Region222 – 2276Interaction with ubiquitin By similarity

Sites

Active site951Nucleophile By similarity
Active site1721Proton donor By similarity
Site1871Important for enzyme activity By similarity

Amino acid modifications

Modified residue1331Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
P58321 [UniParc].

Last modified October 18, 2001. Version 1.
Checksum: 9D7899AD70C27819

FASTA23326,450
        10         20         30         40         50         60 
MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMESE LLSIIPRPVC AVLLLFPITE 

        70         80         90        100        110        120 
KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGT IGLIHAIANN KDKVHFESGS 

       130        140        150        160        170        180 
TLKKFLEESV SMSPEERAKY LENYDAIRVT HETSAHEGQT EAPSIDEKVD LHFIALVHVD 

       190        200        210        220        230 
GHLYELDGWK PFPINHGKTS DETLLEDVIK VCKKFMERDP DELRFNAIAL SAA 

« Hide

References

[1]"Cloning, expression, and mapping of a mouse gene, Uchl4, highly homologous to human and mouse Uchl3."
Osawa Y., Wang Y.-L., Osaka H., Aoki S., Wada K.
Biochem. Biophys. Res. Commun. 283:627-633(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6J.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB035420 mRNA. Translation: BAB47122.1.
CCDSCCDS23278.1.
PIRJC7689.
RefSeqNP_291085.1. NM_033607.1.
UniGeneMm.390180.

3D structure databases

ProteinModelPortalP58321.
SMRP58321. Positions 5-233.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSC12.007.

PTM databases

PhosphoSiteP58321.

Proteomic databases

MaxQBP58321.
PaxDbP58321.
PRIDEP58321.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000039011; ENSMUSP00000045208; ENSMUSG00000035337.
GeneID93841.
KEGGmmu:93841.
UCSCuc009qbq.1. mouse.

Organism-specific databases

CTD93841.
MGIMGI:1890440. Uchl4.

Phylogenomic databases

eggNOGNOG327708.
GeneTreeENSGT00510000046640.
HOGENOMHOG000182400.
HOVERGENHBG075483.
InParanoidP58321.
KOK05609.
OMAYGMESEL.
OrthoDBEOG7S7SFK.
PhylomeDBP58321.
TreeFamTF316166.

Gene expression databases

BgeeP58321.
CleanExMM_UCHL4.
GenevestigatorP58321.

Family and domain databases

Gene3D3.40.532.10. 1 hit.
InterProIPR001578. Peptidase_C12_UCH.
[Graphical view]
PANTHERPTHR10589. PTHR10589. 1 hit.
PfamPF01088. Peptidase_C12. 1 hit.
[Graphical view]
PRINTSPR00707. UBCTHYDRLASE.
ProtoNetSearch...

Other

NextBio351701.
PROP58321.
SOURCESearch...

Entry information

Entry nameUCHL4_MOUSE
AccessionPrimary (citable) accession number: P58321
Entry history
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: October 18, 2001
Last modified: July 9, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot