P58321 (UCHL4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase isozyme L4
Short name=UCH-L4
EC=3.4.19.12

Alternative name(s):
Ubiquitin thioesterase L4

Gene names

Name:

Uchl4

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	233 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ubiquitin-protein hydrolase is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Expressed in various tissues at low level.
Sequence similarities	Belongs to the peptidase C12 family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm
Molecular function	Hydrolase Protease Thiol protease
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 233	233	Ubiquitin carboxyl-terminal hydrolase isozyme L4		PRO_0000211064
Regions
	Region	8 – 13	6	Interaction with ubiquitin By similarity
	Region	222 – 227	6	Interaction with ubiquitin By similarity
Sites
	Active site	95	1	Nucleophile By similarity
	Active site	172	1	Proton donor By similarity
	Site	187	1	Important for enzyme activity By similarity
Amino acid modifications
	Modified residue	133	1	Phosphoserine Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P58321 [UniParc]. Last modified October 18, 2001. Version 1. Checksum: 9D7899AD70C27819	FASTA	233	26,450
	10 20 30 40 50 60 MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMESE LLSIIPRPVC AVLLLFPITE 70 80 90 100 110 120 KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGT IGLIHAIANN KDKVHFESGS 130 140 150 160 170 180 TLKKFLEESV SMSPEERAKY LENYDAIRVT HETSAHEGQT EAPSIDEKVD LHFIALVHVD 190 200 210 220 230 GHLYELDGWK PFPINHGKTS DETLLEDVIK VCKKFMERDP DELRFNAIAL SAA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, expression, and mapping of a mouse gene, Uchl4, highly homologous to human and mouse Uchl3." Osawa Y., Wang Y.-L., Osaka H., Aoki S., Wada K. Biochem. Biophys. Res. Commun. 283:627-633(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6J.
[2]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-133, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB035420 mRNA. Translation: BAB47122.1.
IPI	IPI00118499.
PIR	JC7689.
RefSeq	NP_291085.1. NM_033607.1.
UniGene	Mm.390180.
3D structure databases
ProteinModelPortal	P58321.
SMR	P58321. Positions 5-233.
ModBase	Search...
Protein family/group databases
MEROPS	C12.007.
PTM databases
PhosphoSite	P58321.
Proteomic databases
PaxDb	P58321.
PRIDE	P58321.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000039011; ENSMUSP00000045208; ENSMUSG00000035337.
GeneID	93841.
KEGG	mmu:93841.
Organism-specific databases
CTD	93841.
MGI	MGI:1890440. Uchl4.
Phylogenomic databases
eggNOG	NOG327708.
GeneTree	ENSGT00510000046640.
HOGENOM	HOG000182400.
HOVERGEN	HBG075483.
InParanoid	P58321.
KO	K05609.
OMA	GMESELL.
OrthoDB	EOG4HX51V.
Gene expression databases
ArrayExpress	P58321.
Bgee	P58321.
CleanEx	MM_UCHL4.
Genevestigator	P58321.
GermOnline	ENSMUSG00000035337. Mus musculus.
Family and domain databases
Gene3D	3.40.532.10. 1 hit.
InterPro	IPR001578. Peptidase_C12. [Graphical view]
PANTHER	PTHR10589. PTHR10589. 1 hit.
Pfam	PF01088. Peptidase_C12. 1 hit. [Graphical view]
PRINTS	PR00707. UBCTHYDRLASE.
PROSITE	PS00140. UCH_1. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	351701.
SOURCE	Search...

Entry information

Entry name

UCHL4_MOUSE

Accession

Primary (citable) accession number: P58321

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	October 18, 2001
Last modified:	April 3, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program