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Protein

Ubiquitin carboxyl-terminal hydrolase isozyme L4

Gene

Uchl4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Ubiquitin-protein hydrolase is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei95 – 951NucleophileBy similarity
Active sitei172 – 1721Proton donorBy similarity
Sitei187 – 1871Important for enzyme activityBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC12.007.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase isozyme L4 (EC:3.4.19.12)
Short name:
UCH-L4
Alternative name(s):
Ubiquitin thioesterase L4
Gene namesi
Name:Uchl4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1890440. Uchl4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 233233Ubiquitin carboxyl-terminal hydrolase isozyme L4PRO_0000211064Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei133 – 1331PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP58321.
PaxDbiP58321.
PRIDEiP58321.

PTM databases

PhosphoSiteiP58321.

Expressioni

Tissue specificityi

Expressed in various tissues at low level.

Gene expression databases

BgeeiP58321.
CleanExiMM_UCHL4.
ExpressionAtlasiP58321. baseline and differential.
GenevisibleiP58321. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000045208.

Structurei

3D structure databases

ProteinModelPortaliP58321.
SMRiP58321. Positions 5-233.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni8 – 136Interaction with ubiquitinBy similarity
Regioni222 – 2276Interaction with ubiquitinBy similarity

Sequence similaritiesi

Belongs to the peptidase C12 family.Curated

Phylogenomic databases

eggNOGiNOG327708.
GeneTreeiENSGT00510000046640.
HOGENOMiHOG000182400.
HOVERGENiHBG075483.
InParanoidiP58321.
KOiK05609.
OMAiYIKQTIG.
OrthoDBiEOG7S7SFK.
PhylomeDBiP58321.
TreeFamiTF316166.

Family and domain databases

Gene3Di3.40.532.10. 1 hit.
InterProiIPR001578. Peptidase_C12_UCH.
[Graphical view]
PANTHERiPTHR10589. PTHR10589. 1 hit.
PfamiPF01088. Peptidase_C12. 1 hit.
[Graphical view]
PRINTSiPR00707. UBCTHYDRLASE.

Sequencei

Sequence statusi: Complete.

P58321-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMESE LLSIIPRPVC
60 70 80 90 100
AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGT
110 120 130 140 150
IGLIHAIANN KDKVHFESGS TLKKFLEESV SMSPEERAKY LENYDAIRVT
160 170 180 190 200
HETSAHEGQT EAPSIDEKVD LHFIALVHVD GHLYELDGWK PFPINHGKTS
210 220 230
DETLLEDVIK VCKKFMERDP DELRFNAIAL SAA
Length:233
Mass (Da):26,450
Last modified:October 18, 2001 - v1
Checksum:i9D7899AD70C27819
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB035420 mRNA. Translation: BAB47122.1.
CCDSiCCDS23278.1.
PIRiJC7689.
RefSeqiNP_291085.1. NM_033607.1.
UniGeneiMm.390180.

Genome annotation databases

EnsembliENSMUST00000039011; ENSMUSP00000045208; ENSMUSG00000035337.
GeneIDi93841.
KEGGimmu:93841.
UCSCiuc009qbq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB035420 mRNA. Translation: BAB47122.1.
CCDSiCCDS23278.1.
PIRiJC7689.
RefSeqiNP_291085.1. NM_033607.1.
UniGeneiMm.390180.

3D structure databases

ProteinModelPortaliP58321.
SMRiP58321. Positions 5-233.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000045208.

Protein family/group databases

MEROPSiC12.007.

PTM databases

PhosphoSiteiP58321.

Proteomic databases

MaxQBiP58321.
PaxDbiP58321.
PRIDEiP58321.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000039011; ENSMUSP00000045208; ENSMUSG00000035337.
GeneIDi93841.
KEGGimmu:93841.
UCSCiuc009qbq.1. mouse.

Organism-specific databases

CTDi93841.
MGIiMGI:1890440. Uchl4.

Phylogenomic databases

eggNOGiNOG327708.
GeneTreeiENSGT00510000046640.
HOGENOMiHOG000182400.
HOVERGENiHBG075483.
InParanoidiP58321.
KOiK05609.
OMAiYIKQTIG.
OrthoDBiEOG7S7SFK.
PhylomeDBiP58321.
TreeFamiTF316166.

Miscellaneous databases

NextBioi351701.
PROiP58321.
SOURCEiSearch...

Gene expression databases

BgeeiP58321.
CleanExiMM_UCHL4.
ExpressionAtlasiP58321. baseline and differential.
GenevisibleiP58321. MM.

Family and domain databases

Gene3Di3.40.532.10. 1 hit.
InterProiIPR001578. Peptidase_C12_UCH.
[Graphical view]
PANTHERiPTHR10589. PTHR10589. 1 hit.
PfamiPF01088. Peptidase_C12. 1 hit.
[Graphical view]
PRINTSiPR00707. UBCTHYDRLASE.
ProtoNetiSearch...

Publicationsi

  1. "Cloning, expression, and mapping of a mouse gene, Uchl4, highly homologous to human and mouse Uchl3."
    Osawa Y., Wang Y.-L., Osaka H., Aoki S., Wada K.
    Biochem. Biophys. Res. Commun. 283:627-633(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.

Entry informationi

Entry nameiUCHL4_MOUSE
AccessioniPrimary (citable) accession number: P58321
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: October 18, 2001
Last modified: July 22, 2015
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.