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P58321

- UCHL4_MOUSE

UniProt

P58321 - UCHL4_MOUSE

Protein

Ubiquitin carboxyl-terminal hydrolase isozyme L4

Gene

Uchl4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (18 Oct 2001)
      Previous versions | rss
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    Functioni

    Ubiquitin-protein hydrolase is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin.

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei95 – 951NucleophileBy similarity
    Active sitei172 – 1721Proton donorBy similarity
    Sitei187 – 1871Important for enzyme activityBy similarity

    GO - Molecular functioni

    1. ubiquitin-specific protease activity Source: InterPro

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC12.007.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase isozyme L4 (EC:3.4.19.12)
    Short name:
    UCH-L4
    Alternative name(s):
    Ubiquitin thioesterase L4
    Gene namesi
    Name:Uchl4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:1890440. Uchl4.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 233233Ubiquitin carboxyl-terminal hydrolase isozyme L4PRO_0000211064Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei133 – 1331PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP58321.
    PaxDbiP58321.
    PRIDEiP58321.

    PTM databases

    PhosphoSiteiP58321.

    Expressioni

    Tissue specificityi

    Expressed in various tissues at low level.

    Gene expression databases

    BgeeiP58321.
    CleanExiMM_UCHL4.
    GenevestigatoriP58321.

    Structurei

    3D structure databases

    ProteinModelPortaliP58321.
    SMRiP58321. Positions 5-233.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni8 – 136Interaction with ubiquitinBy similarity
    Regioni222 – 2276Interaction with ubiquitinBy similarity

    Sequence similaritiesi

    Belongs to the peptidase C12 family.Curated

    Phylogenomic databases

    eggNOGiNOG327708.
    GeneTreeiENSGT00510000046640.
    HOGENOMiHOG000182400.
    HOVERGENiHBG075483.
    InParanoidiP58321.
    KOiK05609.
    OMAiYGMESEL.
    OrthoDBiEOG7S7SFK.
    PhylomeDBiP58321.
    TreeFamiTF316166.

    Family and domain databases

    Gene3Di3.40.532.10. 1 hit.
    InterProiIPR001578. Peptidase_C12_UCH.
    [Graphical view]
    PANTHERiPTHR10589. PTHR10589. 1 hit.
    PfamiPF01088. Peptidase_C12. 1 hit.
    [Graphical view]
    PRINTSiPR00707. UBCTHYDRLASE.

    Sequencei

    Sequence statusi: Complete.

    P58321-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMESE LLSIIPRPVC    50
    AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGT 100
    IGLIHAIANN KDKVHFESGS TLKKFLEESV SMSPEERAKY LENYDAIRVT 150
    HETSAHEGQT EAPSIDEKVD LHFIALVHVD GHLYELDGWK PFPINHGKTS 200
    DETLLEDVIK VCKKFMERDP DELRFNAIAL SAA 233
    Length:233
    Mass (Da):26,450
    Last modified:October 18, 2001 - v1
    Checksum:i9D7899AD70C27819
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB035420 mRNA. Translation: BAB47122.1.
    CCDSiCCDS23278.1.
    PIRiJC7689.
    RefSeqiNP_291085.1. NM_033607.1.
    UniGeneiMm.390180.

    Genome annotation databases

    EnsembliENSMUST00000039011; ENSMUSP00000045208; ENSMUSG00000035337.
    GeneIDi93841.
    KEGGimmu:93841.
    UCSCiuc009qbq.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB035420 mRNA. Translation: BAB47122.1 .
    CCDSi CCDS23278.1.
    PIRi JC7689.
    RefSeqi NP_291085.1. NM_033607.1.
    UniGenei Mm.390180.

    3D structure databases

    ProteinModelPortali P58321.
    SMRi P58321. Positions 5-233.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C12.007.

    PTM databases

    PhosphoSitei P58321.

    Proteomic databases

    MaxQBi P58321.
    PaxDbi P58321.
    PRIDEi P58321.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000039011 ; ENSMUSP00000045208 ; ENSMUSG00000035337 .
    GeneIDi 93841.
    KEGGi mmu:93841.
    UCSCi uc009qbq.1. mouse.

    Organism-specific databases

    CTDi 93841.
    MGIi MGI:1890440. Uchl4.

    Phylogenomic databases

    eggNOGi NOG327708.
    GeneTreei ENSGT00510000046640.
    HOGENOMi HOG000182400.
    HOVERGENi HBG075483.
    InParanoidi P58321.
    KOi K05609.
    OMAi YGMESEL.
    OrthoDBi EOG7S7SFK.
    PhylomeDBi P58321.
    TreeFami TF316166.

    Miscellaneous databases

    NextBioi 351701.
    PROi P58321.
    SOURCEi Search...

    Gene expression databases

    Bgeei P58321.
    CleanExi MM_UCHL4.
    Genevestigatori P58321.

    Family and domain databases

    Gene3Di 3.40.532.10. 1 hit.
    InterProi IPR001578. Peptidase_C12_UCH.
    [Graphical view ]
    PANTHERi PTHR10589. PTHR10589. 1 hit.
    Pfami PF01088. Peptidase_C12. 1 hit.
    [Graphical view ]
    PRINTSi PR00707. UBCTHYDRLASE.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, expression, and mapping of a mouse gene, Uchl4, highly homologous to human and mouse Uchl3."
      Osawa Y., Wang Y.-L., Osaka H., Aoki S., Wada K.
      Biochem. Biophys. Res. Commun. 283:627-633(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6J.

    Entry informationi

    Entry nameiUCHL4_MOUSE
    AccessioniPrimary (citable) accession number: P58321
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 18, 2001
    Last sequence update: October 18, 2001
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3