P58314 (ALF1_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fructose-bisphosphate aldolase class 1
EC=4.1.2.13

Alternative name(s):
Fructose-biphosphate aldolase class I
Short name=FBP aldolase

Gene names

Name:	fba
Ordered Locus Names:	PF1956

Organism

Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

Taxonomic identifier

186497 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.
Enzyme regulation	Activated by citrate.
Subunit structure	Homooctamer.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the DeoC/FbaB aldolase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	fructose-bisphosphate aldolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 281	281	Fructose-bisphosphate aldolase class 1		PRO_0000138949
Sites
	Active site	191	1	Schiff-base intermediate with dihydroxyacetone-P
Experimental info
	Mutagenesis	191	1	K → A: Loss of activity. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P58314 [UniParc]. Last modified October 18, 2001. Version 1. Checksum: 19637AD0DA8CBD2F	FASTA	281	31,135
	10 20 30 40 50 60 MEAPQNVGIK RRLKRFFRRD GRALIFAMDH GFEHGPTDFE PVWEHVNPRV IIRKVVRAGI 70 80 90 100 110 120 DGVMMLPGLA RIAGDEVKPE VGLMIKLTSK TNLRPKPEQL LQSQLGFVDD AIKLGADAIA 130 140 150 160 170 180 ATVYWGSPQE DVMMRQFAEI VSYAHDLGYP VVQFAYPRGP YIDEKYGKKE DYRVVMYGAR 190 200 210 220 230 240 AAAESGADMI KTYWTGSKET FAKVVEAAAG VPVLLSGGAK TENPVDFLKV VWEVIEAGGA 250 260 270 280 GAVVGRNIFQ RENPEPMIKA LIRVIHRNED PEEAAKAEGL I

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Archaeal fructose-1,6-bisphosphate aldolases constitute a new family of archaeal type class I aldolases." Siebers B., Brinkmann H., Doerr C., Tjaden B., Lilie H., van der Oost J., Verhees C.H. J. Biol. Chem. 276:28710-28718(2001) [PubMed: 11387336] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION, MUTAGENESIS OF LYS-191. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
[2]	"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences." Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T. Genetics 152:1299-1305(1999) [PubMed: 10430560] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF368256 Genomic DNA. Translation: AAK83936.1. AE009950 Genomic DNA. Translation: AAL82080.1.
RefSeq	NP_579685.1. NC_003413.1.
3D structure databases
ProteinModelPortal	P58314.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000004924; EBPYRP00000004785; EBPYRG00000004923.
GeneID	1469838.
GenomeReviews	Gene locus PF1956 in contig AE009950_GR.
KEGG	pfu:PF1956.
NMPDR	fig|186497.1.peg.2008.
Phylogenomic databases
GeneTree	EBGT00050000022638.
HOGENOM	HBG553580.
OMA	RVIHRNE.
PhylomeDB	P58314.
ProtClustDB	PRK07226.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-11810.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR002915. DeoC/AroFGH_arch. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01623.
Pfam	PF01791. DeoC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALF1_PYRFU

Accession

Primary (citable) accession number: P58314

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	October 18, 2001
Last modified:	December 14, 2011
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program