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P58314

- ALF1_PYRFU

UniProt

P58314 - ALF1_PYRFU

Protein

Fructose-bisphosphate aldolase class 1

Gene

fba

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (18 Oct 2001)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

    Enzyme regulationi

    Activated by citrate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei191 – 1911Schiff-base intermediate with dihydroxyacetone-P

    GO - Molecular functioni

    1. fructose-bisphosphate aldolase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    Schiff base

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-11810.
    SABIO-RKP58314.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fructose-bisphosphate aldolase class 1 (EC:4.1.2.13)
    Alternative name(s):
    Fructose-bisphosphate aldolase class I
    Short name:
    FBP aldolase
    Gene namesi
    Name:fba
    Ordered Locus Names:PF1956
    OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
    Taxonomic identifieri186497 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    ProteomesiUP000001013: Chromosome

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi191 – 1911K → A: Loss of activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 281281Fructose-bisphosphate aldolase class 1PRO_0000138949Add
    BLAST

    Proteomic databases

    PRIDEiP58314.

    Interactioni

    Subunit structurei

    Homooctamer.

    Protein-protein interaction databases

    STRINGi186497.PF1956.

    Structurei

    3D structure databases

    ProteinModelPortaliP58314.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the DeoC/FbaB aldolase family.Curated

    Phylogenomic databases

    eggNOGiCOG1830.
    HOGENOMiHOG000224790.
    KOiK11645.
    OMAiATEGEYI.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR002915. DeoC/FbaB/lacD_aldolase.
    [Graphical view]
    PfamiPF01791. DeoC. 1 hit.
    [Graphical view]
    PIRSFiPIRSF038992. Aldolase_Ia. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P58314-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEAPQNVGIK RRLKRFFRRD GRALIFAMDH GFEHGPTDFE PVWEHVNPRV    50
    IIRKVVRAGI DGVMMLPGLA RIAGDEVKPE VGLMIKLTSK TNLRPKPEQL 100
    LQSQLGFVDD AIKLGADAIA ATVYWGSPQE DVMMRQFAEI VSYAHDLGYP 150
    VVQFAYPRGP YIDEKYGKKE DYRVVMYGAR AAAESGADMI KTYWTGSKET 200
    FAKVVEAAAG VPVLLSGGAK TENPVDFLKV VWEVIEAGGA GAVVGRNIFQ 250
    RENPEPMIKA LIRVIHRNED PEEAAKAEGL I 281
    Length:281
    Mass (Da):31,135
    Last modified:October 18, 2001 - v1
    Checksum:i19637AD0DA8CBD2F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF368256 Genomic DNA. Translation: AAK83936.1.
    AE009950 Genomic DNA. Translation: AAL82080.1.
    RefSeqiNP_579685.1. NC_003413.1.
    WP_011013098.1. NC_003413.1.

    Genome annotation databases

    EnsemblBacteriaiAAL82080; AAL82080; PF1956.
    GeneIDi1469838.
    KEGGipfu:PF1956.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF368256 Genomic DNA. Translation: AAK83936.1 .
    AE009950 Genomic DNA. Translation: AAL82080.1 .
    RefSeqi NP_579685.1. NC_003413.1.
    WP_011013098.1. NC_003413.1.

    3D structure databases

    ProteinModelPortali P58314.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 186497.PF1956.

    Proteomic databases

    PRIDEi P58314.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL82080 ; AAL82080 ; PF1956 .
    GeneIDi 1469838.
    KEGGi pfu:PF1956.

    Phylogenomic databases

    eggNOGi COG1830.
    HOGENOMi HOG000224790.
    KOi K11645.
    OMAi ATEGEYI.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-11810.
    SABIO-RK P58314.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR002915. DeoC/FbaB/lacD_aldolase.
    [Graphical view ]
    Pfami PF01791. DeoC. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF038992. Aldolase_Ia. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Archaeal fructose-1,6-bisphosphate aldolases constitute a new family of archaeal type class I aldolases."
      Siebers B., Brinkmann H., Doerr C., Tjaden B., Lilie H., van der Oost J., Verhees C.H.
      J. Biol. Chem. 276:28710-28718(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION, MUTAGENESIS OF LYS-191.
      Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
    2. "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
      Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
      Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

    Entry informationi

    Entry nameiALF1_PYRFU
    AccessioniPrimary (citable) accession number: P58314
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 18, 2001
    Last sequence update: October 18, 2001
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3