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Protein

Sodium/potassium-transporting ATPase subunit alpha-3

Gene

atp1a3

Organism
Oreochromis mossambicus (Mozambique tilapia) (Tilapia mossambica)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

Catalytic activityi

ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei3634-aspartylphosphate intermediateBy similarity1
Metal bindingi704MagnesiumBy similarity1
Metal bindingi708MagnesiumBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/potassium-transporting ATPase subunit alpha-3 (EC:3.6.3.9)
Short name:
Na(+)/K(+) ATPase alpha-3 subunit
Alternative name(s):
Na(+)/K(+) ATPase alpha(III) subunit
Sodium pump subunit alpha-3
Gene namesi
Name:atp1a3
OrganismiOreochromis mossambicus (Mozambique tilapia) (Tilapia mossambica)
Taxonomic identifieri8127 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiNeoteleosteiAcanthomorphataOvalentariaCichlomorphaeCichliformesCichlidaeAfrican cichlidsPseudocrenilabrinaeOreochrominiOreochromis

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 74CytoplasmicSequence analysisAdd BLAST74
Transmembranei75 – 95HelicalSequence analysisAdd BLAST21
Topological domaini96 – 118ExtracellularSequence analysisAdd BLAST23
Transmembranei119 – 139HelicalSequence analysisAdd BLAST21
Topological domaini140 – 275CytoplasmicSequence analysisAdd BLAST136
Transmembranei276 – 295HelicalSequence analysisAdd BLAST20
Topological domaini296 – 307ExtracellularSequence analysisAdd BLAST12
Transmembranei308 – 325HelicalSequence analysisAdd BLAST18
Topological domaini326 – 759CytoplasmicSequence analysisAdd BLAST434
Transmembranei760 – 779HelicalSequence analysisAdd BLAST20
Topological domaini780 – 789ExtracellularSequence analysis10
Transmembranei790 – 810HelicalSequence analysisAdd BLAST21
Topological domaini811 – 830CytoplasmicSequence analysisAdd BLAST20
Transmembranei831 – 853HelicalSequence analysisAdd BLAST23
Topological domaini854 – 905ExtracellularSequence analysisAdd BLAST52
Transmembranei906 – 925HelicalSequence analysisAdd BLAST20
Topological domaini926 – 938CytoplasmicSequence analysisAdd BLAST13
Transmembranei939 – 957HelicalSequence analysisAdd BLAST19
Topological domaini958 – 972ExtracellularSequence analysisAdd BLAST15
Transmembranei973 – 993HelicalSequence analysisAdd BLAST21
Topological domaini994 – 1010CytoplasmicSequence analysisAdd BLAST17

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000463021 – 1010Sodium/potassium-transporting ATPase subunit alpha-3Add BLAST1010

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei930Phosphoserine; by PKABy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP58312.

Interactioni

Subunit structurei

Composed of three subunits: alpha (catalytic), beta and gamma.

Structurei

3D structure databases

ProteinModelPortaliP58312.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni69 – 71Interaction with phosphoinositide-3 kinaseBy similarity3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi10 – 13Poly-Lys4

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG004298.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005775. P-type_ATPase_IIC.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P58312-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDKDDRFPK KKKGGTKDMD ALKKEVPITE HKMSVEEVCR KFQTDVVQGL
60 70 80 90 100
TNAKAAEFLL RDGPNALTPP PTTPEWVKFC RQLFGGFSIL LWTGAILCFL
110 120 130 140 150
AYAIQAATED EPAGDNLYLG IVLTAVVVIT GCFSYFQEAK SSKIMESFKN
160 170 180 190 200
MVPQQALVIR EGEKVQVNAE EVMAGDLIEV KGGDRIPADI RVTSAHGCKV
210 220 230 240 250
DNSSLTGESE PQSRSPDCTH DNPLETRNIA FFSTNCVEGT ARGIVICTGD
260 270 280 290 300
RTVMGRIATL TSGLETGKTP IAVEIEHFIH IITGVAVFLG VTFFILAIIL
310 320 330 340 350
GYTWLKAVIF LIGIIVANVP EGLLATVTVC LTLTAKRMAK KNCLVKNLEA
360 370 380 390 400
VETLGSTSTI CSDKTGTLTQ NRMTVAHMWF DNQIHEADTT EDQSGAAFDK
410 420 430 440 450
SSVTWLSLSR VAPLCNRAQF KPRQDSVSIL KRDVAGDASE SALLKCIELS
460 470 480 490 500
CGSVRMMRDK NKKVAEIPFN PTNKYQLSIH ETEDPNDNRY LLVMKGALER
510 520 530 540 550
ILDRCSTIML QGKEQPMDEE MKEAFQNAYM ELGGLGERVL GFRHLLLPED
560 570 580 590 600
QYPKGFAFDT DDVNFQTDNL CFVGLMSMID PPRAAVPDAV GKCRSAGIKV
610 620 630 640 650
IMVTGDHPIT AKAIAKGVGI ISEGNETVED IAARLNIPVS QVNPRDAKAC
660 670 680 690 700
VIHGSDLKDL SQDQMDDILR NHTEIVFART SPQQKLIIVE GCQRLGAIVA
710 720 730 740 750
VTGDGVNDSP ALKKADIGVA MGISGSDVSK QAADMILLDD NFASIVTGVE
760 770 780 790 800
EGRLIFDNLK KSIAYTLTSN IPEITPFLFF IIVNIPLALG TITILCIDLG
810 820 830 840 850
TDMGSAISLA YETAESDIMK RQPRNPCRDK LVNERLISIA YGQIGMIQAL
860 870 880 890 900
GGFFSYFVIL AENGFLPSQL VGIRLNWDDR SLNDLEDSYG QQWTYEQRKI
910 920 930 940 950
VEFTCHTAFF VSIVVVQWAD LIICKTRRNS VFQQGMKNKI LIFGLFEETA
960 970 980 990 1000
LAAFLSYCPG MDVALRMYPL KPTWWFWAFP YSFLIFVYDE ARKLILCRNP
1010
GGWVEKETYY
Length:1,010
Mass (Da):111,507
Last modified:October 18, 2001 - v1
Checksum:i9FEDB07C7547F0D1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF109409 mRNA. Translation: AAF75108.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF109409 mRNA. Translation: AAF75108.1.

3D structure databases

ProteinModelPortaliP58312.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP58312.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG004298.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005775. P-type_ATPase_IIC.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAT1A3_OREMO
AccessioniPrimary (citable) accession number: P58312
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: October 18, 2001
Last modified: October 5, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.