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Protein

Orexin receptor type 2

Gene

Hcrtr2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Nonselective, high-affinity receptor for both orexin-A and orexin-B neuropeptides.

GO - Molecular functioni

  1. neuropeptide receptor activity Source: GO_Central
  2. orexin receptor activity Source: MGI
  3. peptide binding Source: GO_Central
  4. peptide hormone binding Source: Ensembl

GO - Biological processi

  1. cellular response to hormone stimulus Source: GO_Central
  2. circadian sleep/wake cycle process Source: InterPro
  3. feeding behavior Source: GO_Central
  4. neuropeptide signaling pathway Source: GO_Central
  5. phospholipase C-activating G-protein coupled receptor signaling pathway Source: MGI
  6. regulation of circadian sleep/wake cycle, sleep Source: GO_Central
  7. response to peptide Source: GO_Central
  8. synaptic transmission Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_329572. Orexin and neuropeptides FF and QRFP bind to their respective receptors.
REACT_347934. G alpha (q) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Orexin receptor type 2
Short name:
Ox-2-R
Short name:
Ox2-R
Short name:
Ox2R
Alternative name(s):
Hypocretin receptor type 2
Gene namesi
Name:Hcrtr2
Synonyms:Mox2r
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:2680765. Hcrtr2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5454ExtracellularSequence AnalysisAdd
BLAST
Transmembranei55 – 7521Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini76 – 8813CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei89 – 11022Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini111 – 12717ExtracellularSequence AnalysisAdd
BLAST
Transmembranei128 – 15023Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini151 – 17222CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei173 – 19321Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini194 – 22431ExtracellularSequence AnalysisAdd
BLAST
Transmembranei225 – 24723Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini248 – 30457CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei305 – 32723Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini328 – 34215ExtracellularSequence AnalysisAdd
BLAST
Transmembranei343 – 36624Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini367 – 46094CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 460460Orexin receptor type 2PRO_0000069990Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi14 – 141N-linked (GlcNAc...)Sequence Analysis
Glycosylationi22 – 221N-linked (GlcNAc...)Sequence Analysis
Glycosylationi202 – 2021N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP58308.

PTM databases

PhosphoSiteiP58308.

Expressioni

Tissue specificityi

Widely expressed. Isoform 2 not detected in skeletal muscle and kidney.1 Publication

Gene expression databases

BgeeiP58308.
CleanExiMM_HCRTR2.
ExpressionAtlasiP58308. baseline.
GenevestigatoriP58308.

Interactioni

Structurei

3D structure databases

ProteinModelPortaliP58308.
SMRiP58308. Positions 56-376.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG253280.
GeneTreeiENSGT00760000118781.
HOGENOMiHOG000230498.
HOVERGENiHBG101173.
InParanoidiP58308.
KOiK04239.
OMAiNSIVVIW.
OrthoDBiEOG7CG72H.
PhylomeDBiP58308.
TreeFamiTF315303.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000204. Orexin_rcpt.
IPR004060. Orexin_rcpt_2.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
PF03827. Orexin_rec2. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01522. OREXIN2R.
PR01064. OREXINR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P58308-1) [UniParc]FASTAAdd to basket

Also known as: 2b

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSTKLEDSL SRRNWSSASE LNETQEPFLN PTDYDDEEFL RYLWREYLHP
60 70 80 90 100
KEYEWVLIAG YIIVFVVALI GNVLVCVAVW KNHHMRTVTN YFIVNLSLAD
110 120 130 140 150
VLVTITCLPA TLVVDITETW FFGQSLCKVI PYLQTVSVSV SVLTLSCIAL
160 170 180 190 200
DRWYAICHPL MFKSTAKRAR NSIVVIWIVS CIIMIPQAIV MECSSMLPGL
210 220 230 240 250
ANKTTLFTVC DEHWGGEVYP KMYHICFFLV TYMAPLCLMI LAYLQIFRKL
260 270 280 290 300
WCRQIPGTSS VVQRKWKQQQ PVSQPRGSGQ QSKARISAVA AEIKQIRARR
310 320 330 340 350
KTARMLMVVL LVFAICYLPI SILNVLKRVF GMFTHTEDRE TVYAWFTFSH
360 370 380 390 400
WLVYANSAAN PIIYNFLSGK FREEFKAAFS CCLGVHHRQG DRLARGRTST
410 420 430 440 450
ESRKSLTTQI SNFDNVSKLS EHVVLTSIST LPAANGAGPL QNWYLQQGVP
460
SSLLSTWLEV
Length:460
Mass (Da):52,461
Last modified:May 30, 2003 - v2
Checksum:iD62A67C15BA67DCC
GO
Isoform 2 (identifier: P58308-2) [UniParc]FASTAAdd to basket

Also known as: 2a

The sequence of this isoform differs from the canonical sequence as follows:
     444-460: Missing.

Show »
Length:443
Mass (Da):50,559
Checksum:i794736A669463283
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti201 – 2011A → T in AAK71327 (Ref. 3) Curated
Sequence conflicti240 – 2401I → V in AAK71327 (Ref. 3) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei444 – 46017Missing in isoform 2. 1 PublicationVSP_016466Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY336084 mRNA. Translation: AAR01327.1.
AY336085 mRNA. Translation: AAR01328.1.
AY339390
, AY339383, AY339384, AY339385, AY339386, AY339387, AY339388, AY339389 Genomic DNA. Translation: AAR11293.1.
AY339389
, AY339383, AY339384, AY339385, AY339386, AY339387, AY339388 Genomic DNA. Translation: AAR11294.1.
AK038551 mRNA. Translation: BAC30039.1.
AK048781 mRNA. Translation: BAC33457.1.
AF394597 mRNA. Translation: AAK71327.1.
CCDSiCCDS23350.1. [P58308-1]
RefSeqiNP_945200.1. NM_198962.3. [P58308-1]
UniGeneiMm.335300.

Genome annotation databases

EnsembliENSMUST00000063140; ENSMUSP00000058230; ENSMUSG00000032360. [P58308-1]
ENSMUST00000184757; ENSMUSP00000139377; ENSMUSG00000032360. [P58308-2]
GeneIDi387285.
KEGGimmu:387285.
UCSCiuc009qsz.1. mouse. [P58308-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY336084 mRNA. Translation: AAR01327.1.
AY336085 mRNA. Translation: AAR01328.1.
AY339390
, AY339383, AY339384, AY339385, AY339386, AY339387, AY339388, AY339389 Genomic DNA. Translation: AAR11293.1.
AY339389
, AY339383, AY339384, AY339385, AY339386, AY339387, AY339388 Genomic DNA. Translation: AAR11294.1.
AK038551 mRNA. Translation: BAC30039.1.
AK048781 mRNA. Translation: BAC33457.1.
AF394597 mRNA. Translation: AAK71327.1.
CCDSiCCDS23350.1. [P58308-1]
RefSeqiNP_945200.1. NM_198962.3. [P58308-1]
UniGeneiMm.335300.

3D structure databases

ProteinModelPortaliP58308.
SMRiP58308. Positions 56-376.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

BindingDBiP58308.
ChEMBLiCHEMBL2434818.
GuidetoPHARMACOLOGYi322.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP58308.

Proteomic databases

PRIDEiP58308.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000063140; ENSMUSP00000058230; ENSMUSG00000032360. [P58308-1]
ENSMUST00000184757; ENSMUSP00000139377; ENSMUSG00000032360. [P58308-2]
GeneIDi387285.
KEGGimmu:387285.
UCSCiuc009qsz.1. mouse. [P58308-1]

Organism-specific databases

CTDi3062.
MGIiMGI:2680765. Hcrtr2.

Phylogenomic databases

eggNOGiNOG253280.
GeneTreeiENSGT00760000118781.
HOGENOMiHOG000230498.
HOVERGENiHBG101173.
InParanoidiP58308.
KOiK04239.
OMAiNSIVVIW.
OrthoDBiEOG7CG72H.
PhylomeDBiP58308.
TreeFamiTF315303.

Enzyme and pathway databases

ReactomeiREACT_329572. Orexin and neuropeptides FF and QRFP bind to their respective receptors.
REACT_347934. G alpha (q) signalling events.

Miscellaneous databases

ChiTaRSiHcrtr2. mouse.
NextBioi405814.
PROiP58308.
SOURCEiSearch...

Gene expression databases

BgeeiP58308.
CleanExiMM_HCRTR2.
ExpressionAtlasiP58308. baseline.
GenevestigatoriP58308.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000204. Orexin_rcpt.
IPR004060. Orexin_rcpt_2.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
PF03827. Orexin_rec2. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01522. OREXIN2R.
PR01064. OREXINR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genomic organization of mouse orexin receptors: characterization of two novel tissue-specific splice variants."
    Chen J., Randeva H.S.
    Mol. Endocrinol. 18:2790-2804(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
    Strain: BALB/c.
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Cerebellum and Hypothalamus.
  3. "Cloning of mouse orexin receptors."
    Szendro P.I., Maevers K., Eichele G.
    Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 100-311 (ISOFORMS 1/2).
    Strain: C57BL/6.
  4. "Hypocretin/orexin, sleep and narcolepsy."
    Hungs M., Mignot E.
    Bioessays 23:397-408(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  5. "To eat or to sleep? Orexin in the regulation of feeding and wakefulness."
    Willie J.T., Chemelli R.M., Sinton C.M., Yanagisawa M.
    Annu. Rev. Neurosci. 24:429-458(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiOX2R_MOUSE
AccessioniPrimary (citable) accession number: P58308
Secondary accession number(s): Q5SGC8, Q6VLX3, Q8BG12
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: May 30, 2003
Last modified: April 1, 2015
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.