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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei75SubstrateUniRule annotation1
Metal bindingi216ManganeseUniRule annotation1
Metal bindingi236Manganese; via tele nitrogenUniRule annotation1
Binding sitei258SubstrateUniRule annotation1
Active sitei260UniRule annotation1
Metal bindingi287ManganeseUniRule annotation1
Binding sitei376GTPUniRule annotation1
Binding sitei407GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi259 – 264GTPUniRule annotation6
Nucleotide bindingi500 – 503GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:TV0200
ORF Names:TVG0204657
OrganismiThermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)
Taxonomic identifieri273116 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
Proteomesi
  • UP000001017 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001036251 – 589Phosphoenolpyruvate carboxykinase [GTP]Add BLAST589

Interactioni

Protein-protein interaction databases

STRINGi273116.TVN0200.

Structurei

3D structure databases

ProteinModelPortaliP58306.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni207 – 209Substrate bindingUniRule annotation3
Regioni374 – 376Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG05865. Archaea.
COG1274. LUCA.
KOiK01596.
OMAiYGENMRV.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiIPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.

Sequencei

Sequence statusi: Complete.

P58306-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLALDETEIN DGAKRWIVSI AKHLNPSSIY VCDGTKEEFD DLASAMVKDG
60 70 80 90 100
EMIKLNEKNF KNSYLYRSNA TDVARTEERT FISARDKSFV GPLNNFLPLD
110 120 130 140 150
RVKQVWNQFF KGAYTGKTMF VIPYALSSPE SKFADFGIEV TDSKYVVLNL
160 170 180 190 200
HYITRMGKGV IEKIGDRFIK GVHATGNLDP GNKFIIHMPW EKPEGVDADI
210 220 230 240 250
LSVNTNYGGN ALLSKKCHAL RIASVRAREE GWLAEHMLLL EVKDPQGKSH
260 270 280 290 300
FITGAFPSAS GKTNLAMISP PKDYRDAGWS TRLISDDISW IKIVDGKFMA
310 320 330 340 350
TNPENGFFAV VPGTNYNTNK NAMATLSKNT IFTNVGLTMS GDPWWEGLDP
360 370 380 390 400
VYDELYDWKG NKRKIGGEPI AHPNSRYTSP LTNYPYLSDL YEDPEGVPVS
410 420 430 440 450
AILFGGRRAT LIPLVFEAFN WNHGVFLGAT MGVERTAASE GKVGDLRRDP
460 470 480 490 500
MAMRPFCGYN INEYFRHWIE IGERSKNKPK IFYVNWFRRR ADGTFIWPGF
510 520 530 540 550
AENFRVLEWI IYRTSHNDNA VETPIGYIPE SLNLAGLNIS EEDVKELFRI
560 570 580
DKEGWKEEMN EIQKYFSELG NVPEEILKEF ELEKKRLGY
Length:589
Mass (Da):66,908
Last modified:September 26, 2001 - v1
Checksum:iE339F1A18012962B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000011 Genomic DNA. Translation: BAB59342.1.
RefSeqiWP_010916456.1. NC_002689.2.

Genome annotation databases

EnsemblBacteriaiBAB59342; BAB59342; BAB59342.
GeneIDi1441686.
KEGGitvo:TVG0204657.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000011 Genomic DNA. Translation: BAB59342.1.
RefSeqiWP_010916456.1. NC_002689.2.

3D structure databases

ProteinModelPortaliP58306.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273116.TVN0200.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB59342; BAB59342; BAB59342.
GeneIDi1441686.
KEGGitvo:TVG0204657.

Phylogenomic databases

eggNOGiarCOG05865. Archaea.
COG1274. LUCA.
KOiK01596.
OMAiYGENMRV.

Enzyme and pathway databases

UniPathwayiUPA00138.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiIPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPCKG_THEVO
AccessioniPrimary (citable) accession number: P58306
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: September 26, 2001
Last modified: November 2, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.