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Protein

Sodium- and chloride-dependent glycine transporter 2

Gene

Slc6a5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Terminates the action of glycine by its high affinity sodium-dependent reuptake into presynaptic terminals. May be responsible for the termination of neurotransmission at strychnine-sensitive glycinergic synapses.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi208 – 2081Sodium 1; via carbonyl oxygenBy similarity
Metal bindingi210 – 2101Sodium 2; via carbonyl oxygenBy similarity
Metal bindingi211 – 2111Sodium 1; via carbonyl oxygenBy similarity
Metal bindingi215 – 2151Sodium 2By similarity
Metal bindingi479 – 4791Sodium 2By similarity
Metal bindingi511 – 5111Sodium 2By similarity
Metal bindingi576 – 5761Sodium 1; via carbonyl oxygenBy similarity
Metal bindingi579 – 5791Sodium 1By similarity

GO - Molecular functioni

GO - Biological processi

  • glycine transport Source: RGD
Complete GO annotation...

Keywords - Biological processi

Neurotransmitter transport, Symport, Transport

Keywords - Ligandi

Metal-binding, Sodium

Enzyme and pathway databases

ReactomeiR-RNO-442660. Na+/Cl- dependent neurotransmitter transporters.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium- and chloride-dependent glycine transporter 2
Short name:
GlyT-2
Short name:
GlyT2
Alternative name(s):
Solute carrier family 6 member 5
Gene namesi
Name:Slc6a5
Synonyms:Glyt2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi621824. Slc6a5.

Subcellular locationi

  • Cell membrane 1 Publication; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 201201CytoplasmicSequence analysisAdd
BLAST
Transmembranei202 – 22221Helical; Name=1Sequence analysisAdd
BLAST
Transmembranei230 – 24920Helical; Name=2Sequence analysisAdd
BLAST
Transmembranei273 – 29321Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini294 – 395102ExtracellularSequence analysisAdd
BLAST
Transmembranei396 – 41419Helical; Name=4Sequence analysisAdd
BLAST
Transmembranei423 – 44018Helical; Name=5Sequence analysisAdd
BLAST
Transmembranei476 – 49318Helical; Name=6Sequence analysisAdd
BLAST
Transmembranei505 – 52622Helical; Name=7Sequence analysisAdd
BLAST
Transmembranei559 – 57820Helical; Name=8Sequence analysisAdd
BLAST
Transmembranei606 – 62419Helical; Name=9Sequence analysisAdd
BLAST
Transmembranei640 – 66021Helical; Name=10Sequence analysisAdd
BLAST
Transmembranei681 – 70020Helical; Name=11Sequence analysisAdd
BLAST
Transmembranei719 – 73719Helical; Name=12Sequence analysisAdd
BLAST
Topological domaini738 – 79962CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL3195.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 799799Sodium- and chloride-dependent glycine transporter 2PRO_0000214764Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei58 – 581PhosphoserineCombined sources
Modified residuei59 – 591PhosphothreonineBy similarity
Modified residuei86 – 861PhosphoserineBy similarity
Disulfide bondi313 ↔ 322By similarity
Glycosylationi345 – 3451N-linked (GlcNAc...)Sequence analysis
Glycosylationi355 – 3551N-linked (GlcNAc...)Sequence analysis
Glycosylationi360 – 3601N-linked (GlcNAc...)Sequence analysis
Glycosylationi366 – 3661N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP58295.
PRIDEiP58295.

PTM databases

iPTMnetiP58295.

Expressioni

Tissue specificityi

Specifically expressed in spinal cord, brain stem, and to a lesser extent in the cerebellum.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000043665.

Chemistry

BindingDBiP58295.

Structurei

3D structure databases

ProteinModelPortaliP58295.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3660. Eukaryota.
COG0733. LUCA.
GeneTreeiENSGT00760000118857.
HOGENOMiHOG000116406.
HOVERGENiHBG071421.
InParanoidiP58295.
KOiK05038.

Family and domain databases

InterProiIPR000175. Na/ntran_symport.
[Graphical view]
PANTHERiPTHR11616. PTHR11616. 2 hits.
PfamiPF00209. SNF. 1 hit.
[Graphical view]
PRINTSiPR00176. NANEUSMPORT.
PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: P58295-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDCSAPKEMN KPPTNILEAT VPGHRDSPRA PRTSPEQDLP AAAPAAAVQP
60 70 80 90 100
PRVPRSASTG AQTFQSADAR ACEAQRPGVG FCKLSSPQAQ ATSAALRDLS
110 120 130 140 150
EGHSAQANPP SGAAGAGNAL HCKIPALRGP EEDENVSVGK GTLEHNNTPA
160 170 180 190 200
VGWVNMSQST VVLGTDGIAS VLPGSVATTT IPEDEQGDEN KARGNWSSKL
210 220 230 240 250
DFILSMVGYA VGLGNVWRFP YLAFQNGGGA FLIPYLMMLA LAGLPIFFLE
260 270 280 290 300
VSLGQFASQG PVSVWKAIPA LQGCGIAMLI ISVLIAIYYN VIICYTLFYL
310 320 330 340 350
FASFVSVLPW GSCNNPWNTP ECKDKTKLLL DSCVIGDHPK IQIKNSTFCM
360 370 380 390 400
TAYPNLTMVN FTSQANKTFV SGSEEYFKYF VLKISAGIEY PGEIRWPLAF
410 420 430 440 450
CLFLAWVIVY ASLAKGIKTS GKVVYFTATF PYVVLVILLI RGVTLPGAGA
460 470 480 490 500
GIWYFITPKW EKLTDATVWK DAATQIFFSL SAAWGGLITL SSYNKFHNNC
510 520 530 540 550
YRDTLIVTCT NSATSIFAGF VIFSVIGFMA NERKVNIENV ADQGPGIAFV
560 570 580 590 600
VYPEALTRLP LSPFWAIIFF LMLLTLGLDT MFATIETIVT SISDEFPKYL
610 620 630 640 650
RTHKPVFTLG CCICFFIMGF PMITQGGIYM FQLVDTYAAS YALVIIAIFE
660 670 680 690 700
LVGISYVYGL QRFCEDIEMM IGFQPNIFWK VCWAFVTPTI LTFILCFSFY
710 720 730 740 750
QWEPMTYGSY RYPNWSMVLG WLMLACSVIW IPIMFVIKMY LAPGRFIERL
760 770 780 790
KLVCSPQPDW GPFLAQHRGE RYKNMIDPLG TSSLGLKLPV KDLELGTQC
Length:799
Mass (Da):87,842
Last modified:January 20, 2016 - v2
Checksum:i5F0D87B3D2DBE662
GO
Isoform b (identifier: P58295-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-8: Missing.

Show »
Length:791
Mass (Da):86,980
Checksum:i92D1A1C302EF3726
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti139 – 1391G → A in AAS19315 (PubMed:8226790).Curated
Sequence conflicti139 – 1391G → A in AAS49497 (Ref. 2) Curated
Sequence conflicti150 – 1501A → P in AAS19315 (PubMed:8226790).Curated
Sequence conflicti150 – 1501A → P in AAS49497 (Ref. 2) Curated
Sequence conflicti399 – 3991A → P in AAS19315 (PubMed:8226790).Curated
Sequence conflicti399 – 3991A → P in AAS49497 (Ref. 2) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 88Missing in isoform b. 1 PublicationVSP_011610

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L21672 mRNA. Translation: AAS19315.1.
AY547309 mRNA. Translation: AAS49497.1.
AC141159 Genomic DNA. No translation available.
PIRiA48716.
RefSeqiNP_976079.1. NM_203334.1.
UniGeneiRn.34566.

Genome annotation databases

EnsembliENSRNOT00000041950; ENSRNOP00000043665; ENSRNOG00000031662. [P58295-1]
ENSRNOT00000089693; ENSRNOP00000074102; ENSRNOG00000031662. [P58295-2]
GeneIDi171148.
KEGGirno:171148.
UCSCiRGD:621824. rat. [P58295-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L21672 mRNA. Translation: AAS19315.1.
AY547309 mRNA. Translation: AAS49497.1.
AC141159 Genomic DNA. No translation available.
PIRiA48716.
RefSeqiNP_976079.1. NM_203334.1.
UniGeneiRn.34566.

3D structure databases

ProteinModelPortaliP58295.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000043665.

Chemistry

BindingDBiP58295.
ChEMBLiCHEMBL3195.

PTM databases

iPTMnetiP58295.

Proteomic databases

PaxDbiP58295.
PRIDEiP58295.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000041950; ENSRNOP00000043665; ENSRNOG00000031662. [P58295-1]
ENSRNOT00000089693; ENSRNOP00000074102; ENSRNOG00000031662. [P58295-2]
GeneIDi171148.
KEGGirno:171148.
UCSCiRGD:621824. rat. [P58295-1]

Organism-specific databases

CTDi9152.
RGDi621824. Slc6a5.

Phylogenomic databases

eggNOGiKOG3660. Eukaryota.
COG0733. LUCA.
GeneTreeiENSGT00760000118857.
HOGENOMiHOG000116406.
HOVERGENiHBG071421.
InParanoidiP58295.
KOiK05038.

Enzyme and pathway databases

ReactomeiR-RNO-442660. Na+/Cl- dependent neurotransmitter transporters.

Miscellaneous databases

NextBioi35575857.
PROiP58295.

Family and domain databases

InterProiIPR000175. Na/ntran_symport.
[Graphical view]
PANTHERiPTHR11616. PTHR11616. 2 hits.
PfamiPF00209. SNF. 1 hit.
[Graphical view]
PRINTSiPR00176. NANEUSMPORT.
PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of a spinal cord- and brain-specific glycine transporter with novel structural features."
    Liu Q.-R., Lopez-Corcuera B., Mandiyan S., Nelson H., Nelson N.
    J. Biol. Chem. 268:22802-22808(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, SUBCELLULAR LOCATION.
    Tissue: Brain.
  2. "Isoforms of sodium dependent type 2 glycine transporter."
    Liu Q.-R., Li Q.-F.
    Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
    Strain: Sprague-Dawley.
  3. "Genome sequence of the Brown Norway rat yields insights into mammalian evolution."
    Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M.
    , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S.
    Nature 428:493-521(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  4. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-58, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSC6A5_RAT
AccessioniPrimary (citable) accession number: P58295
Secondary accession number(s): F1LNM6, Q6QDB3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: January 20, 2016
Last modified: May 11, 2016
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.