Reviewed, UniProtKB/Swiss-Prot P58284 (MTA1_AZOBR)

Names and origin

Protein names

Recommended name:
    Modification methylase AbrI
      Short name=M.AbrI
    EC=2.1.1.72
Alternative name(s):
    Adenine-specific methyltransferase AbrI

Gene names

Name:

abrIM

Organism

Azospirillum brasilense

Taxonomic identifier

192 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Rhodospirillaceae › Azospirillum

Protein attributes

Sequence length	140 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence CTCGAG, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the AbrI endonuclease Potential.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 140	›140	Modification methylase AbrI		PRO_0000087942
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P58284-1 [UniParc]. Last modified September 26, 2001. Version 1. Checksum: 7B5BC7D96697E174	FASTA	140	15,900
	10 20 30 40 50 60 YRTIDRIYPA LTREHKLLIP DIKGEAHIVY EEGRLYPHHN LYYITSEIWD LRALQAVLLS 70 80 90 100 110 120 GIARLFVSVY STKMHGGFLR FQAQYLRRIR VPNWSQVPAA VRQELITAGA KPDLAACNRA 130 140 VFALYAMTAE ERAALGGNGD

References

[1]	"AbrI restriction modification system from Azospirillium brasilense, molecular cloning and characterization of its genes." Schwabe G., Helke A., Klingmueller W. Submitted (OCT-1991) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 29711 / DSM 1844.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X62690 Genomic DNA. No translation available.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.1.1.72. 1315.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

MTA1_AZOBR

Accession

Primary (citable) accession number: P58284

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	September 26, 2001
Last modified:	January 20, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)