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Protein

Modification methylase AbrI

Gene

abrIM

Organism
Azospirillum brasilense
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence CTCGAG, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the AbrI endonuclease.Curated

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi4924. M.AbrI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase AbrI (EC:2.1.1.72)
Short name:
M.AbrI
Alternative name(s):
Adenine-specific methyltransferase AbrI
Gene namesi
Name:abrIM
OrganismiAzospirillum brasilense
Taxonomic identifieri192 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesRhodospirillaceaeAzospirillum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 140›140Modification methylase AbrIPRO_0000087942Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP58284.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Sequencei

Sequence statusi: Fragment.

P58284-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
YRTIDRIYPA LTREHKLLIP DIKGEAHIVY EEGRLYPHHN LYYITSEIWD
60 70 80 90 100
LRALQAVLLS GIARLFVSVY STKMHGGFLR FQAQYLRRIR VPNWSQVPAA
110 120 130 140
VRQELITAGA KPDLAACNRA VFALYAMTAE ERAALGGNGD
Length:140
Mass (Da):15,900
Last modified:September 26, 2001 - v1
Checksum:i7B5BC7D96697E174
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62690 Genomic DNA. No translation available.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62690 Genomic DNA. No translation available.

3D structure databases

ProteinModelPortaliP58284.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi4924. M.AbrI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

ProtoNetiSearch...

Publicationsi

  1. "AbrI restriction modification system from Azospirillium brasilense, molecular cloning and characterization of its genes."
    Schwabe G., Helke A., Klingmueller W.
    Submitted (OCT-1991) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 29711 / DSM 1844.

Entry informationi

Entry nameiMTA1_AZOBR
AccessioniPrimary (citable) accession number: P58284
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: September 26, 2001
Last modified: October 1, 2014
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.