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P58281

- OPA1_MOUSE

UniProt

P58281 - OPA1_MOUSE

Protein

Dynamin-like 120 kDa protein, mitochondrial

Gene

Opa1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (26 Sep 2001)
      Previous versions | rss
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    Functioni

    Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.
    Dynamin-like 120 kDa protein, form S1: Inactive form produced by cleavage at S1 position by OMA1 following stress conditions that induce loss of mitochondrial membrane potential, leading to negative regulation of mitochondrial fusion.

    Catalytic activityi

    GTP + H2O = GDP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi295 – 3028GTPSequence Analysis
    Nucleotide bindingi398 – 4025GTPSequence Analysis
    Nucleotide bindingi467 – 4704GTPSequence Analysis

    GO - Molecular functioni

    1. GTPase activity Source: UniProtKB
    2. GTP binding Source: UniProtKB-KW
    3. protein binding Source: UniProtKB

    GO - Biological processi

    1. apoptotic process Source: UniProtKB-KW
    2. GTP catabolic process Source: GOC
    3. inner mitochondrial membrane organization Source: UniProtKB
    4. mitochondrial fusion Source: MGI
    5. mitochondrion organization Source: UniProtKB
    6. negative regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
    7. negative regulation of release of cytochrome c from mitochondria Source: UniProtKB
    8. neural tube closure Source: MGI
    9. response to stimulus Source: UniProtKB-KW
    10. visual perception Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Apoptosis, Sensory transduction, Vision

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dynamin-like 120 kDa protein, mitochondrial (EC:3.6.5.5)
    Alternative name(s):
    Large GTP-binding protein
    Short name:
    LargeG
    Optic atrophy protein 1 homolog
    Cleaved into the following chain:
    Gene namesi
    Name:Opa1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 16

    Organism-specific databases

    MGIiMGI:1921393. Opa1.

    Subcellular locationi

    Mitochondrion inner membrane 1 Publication; Single-pass membrane protein 1 Publication. Mitochondrion intermembrane space 1 Publication

    GO - Cellular componenti

    1. dendrite Source: UniProtKB
    2. integral component of membrane Source: UniProtKB-KW
    3. mitochondrial crista Source: UniProtKB
    4. mitochondrial inner membrane Source: UniProtKB
    5. mitochondrial intermembrane space Source: UniProtKB
    6. mitochondrial outer membrane Source: UniProtKB
    7. mitochondrion Source: MGI

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    Pathology & Biotechi

    Disruption phenotypei

    Embryonic fibroblasts show a defect in apoptosis in response to intrinsic signals. This defect can be complemented by a soluble form of Opa1 targeted to the mitochondrial intermembrane space.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 8787MitochondrionBy similarityAdd
    BLAST
    Chaini88 – 960873Dynamin-like 120 kDa protein, mitochondrialPRO_0000007398Add
    BLAST
    Chaini195 – 960766Dynamin-like 120 kDa protein, form S1By similarityPRO_0000253480Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei228 – 2281N6-acetyllysineBy similarity

    Post-translational modificationi

    PARL-dependent proteolytic processing releases an antiapoptotic soluble form not required for mitochondrial fusion. Cleaved by OMA1 at position S1 following stress conditions.2 Publications

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP58281.
    PaxDbiP58281.
    PRIDEiP58281.

    PTM databases

    PhosphoSiteiP58281.

    Expressioni

    Gene expression databases

    ArrayExpressiP58281.
    BgeeiP58281.
    CleanExiMM_OPA1.
    GenevestigatoriP58281.

    Interactioni

    Subunit structurei

    Oligomeric complex consisting of membrane-bound and soluble forms of OPA1. Interacts with CHCHD3 and IMMT; these interactions occur preferentially with soluble OPA1 forms. Binds PARL. Interacts with PRELID1.3 Publications

    Protein-protein interaction databases

    IntActiP58281. 7 interactions.
    MINTiMINT-4105859.

    Structurei

    3D structure databases

    ProteinModelPortaliP58281.
    SMRiP58281. Positions 262-601.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini88 – 969Mitochondrial matrixSequence Analysis
    Topological domaini114 – 960847Mitochondrial intermembraneSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei97 – 11317HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini285 – 561277Dynamin-type GAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili210 – 25445Sequence AnalysisAdd
    BLAST
    Coiled coili895 – 96066Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Coiled coil, Transit peptide, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0699.
    GeneTreeiENSGT00550000074851.
    HOVERGENiHBG019108.
    KOiK17079.
    OMAiKRHKWNE.
    OrthoDBiEOG7TMZR4.
    PhylomeDBiP58281.
    TreeFamiTF314250.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR001401. Dynamin_GTPase.
    IPR022812. Dynamin_SF.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR11566. PTHR11566. 1 hit.
    PfamiPF00350. Dynamin_N. 1 hit.
    [Graphical view]
    PRINTSiPR00195. DYNAMIN.
    SMARTiSM00053. DYNc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS51718. G_DYNAMIN_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P58281-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MWRAGRAAVA CEVCQSLVKH SSGIQRNVPL QKLHLVSRSI YRSHHPALKL    50
    QRPQLRTPFQ QFSSLTHLSL HKLKLSPIKY GYQPRRNFWP ARLAARLLKL 100
    RYIILGSAVG GGYTAKKTFD EWKDMIPDLS DYKWIVPDFI WEIDEYIDLE 150
    KIRKALPSSE DLASLAPDLD KITESLSLLK DFFTAGSPGE TAFRATDHGS 200
    ESDKHYRKVS DKEKIDQLQE ELLHTQLKYQ RILERLEKEN KELRKLVLQK 250
    DDKGIHHRKL KKSLIDMYSE VLDVLSDYDA SYNTQDHLPR VVVVGDQSAG 300
    KTSVLEMIAQ ARIFPRGSGE MMTRSPVKVT LSEGPHHVAL FKDSSREFDL 350
    TKEEDLAALR HEIELRMRKN VKEGCTVSPE TISLNVKGPG LQRMVLVDLP 400
    GVINTVTSGM APDTKETIFS ISKAYMQNPN AIILCIQDGS VDAERSIVTD 450
    LVSQMDPHGR RTIFVLTKVD LAEKNVASPS RIQQIIEGKL FPMKALGYFA 500
    VVTGKGNSSE SIEAIREYEE EFFQNSKLLK TSMLKAHQVT TRNLSLAVSD 550
    CFWKMVRESV EQQADSFKAT RFNLETEWKN NYPRLRELDR NELFEKAKNE 600
    ILDEVISLSQ VTPKHWEEIL QQSLWERVST HVIENIYLPA AQTMNSGTFN 650
    TTVDIKLKQW TDKQLPNKAV EVAWETLQEE FSRFMTEPKG KEHDDIFDKL 700
    KEAVKEESIK RHKWNDFAED SLRVIQHNAL EDRSISDKQQ WDAAIYFMEE 750
    ALQGRLKDTE NAIENMIGPD WKKRWMYWKN RTQEQCVHNE TKNELEKMLK 800
    VNDEHPAYLA SDEITTVRKN LESRGVEVDP SLIKDTWHQV YRRHFLKTAL 850
    NHCNLCRRGF YYYQRHFIDS ELECNDVVLF WRIQRMLAIT ANTLRQQLTN 900
    TEVRRLEKNV KEVLEDFAED GEKKVKLLTG KRVQLAEDLK KVREIQEKLD 950
    AFIEALHQEK 960
    Length:960
    Mass (Da):111,339
    Last modified:September 26, 2001 - v1
    Checksum:i0F103FB1FD570F49
    GO
    Isoform 2 (identifier: P58281-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         208-208: K → KGLLGELILLQQQIQEHEEEARRAAGQYSTSYAQQKRK

    Show »
    Length:997
    Mass (Da):115,593
    Checksum:iFADCB1430E585522
    GO

    Sequence cautioni

    The sequence AAH25160.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti236 – 2361L → P in BAC30002. (PubMed:16141072)Curated
    Sequence conflicti330 – 3312TL → NN in BAC32021. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei208 – 2081K → KGLLGELILLQQQIQEHEEE ARRAAGQYSTSYAQQKRK in isoform 2. 1 PublicationVSP_021037

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB044138 mRNA. Translation: BAB59000.1.
    AK029157 mRNA. Translation: BAC26331.1.
    AK038446 mRNA. Translation: BAC30002.1.
    AK044657 mRNA. Translation: BAC32021.1.
    AK050383 mRNA. Translation: BAC34224.1.
    AK145620 mRNA. Translation: BAE26544.1.
    BC025160 mRNA. Translation: AAH25160.1. Different initiation.
    BC138665 mRNA. Translation: AAI38666.1.
    BC145959 mRNA. Translation: AAI45960.1.
    CCDSiCCDS28096.1. [P58281-1]
    RefSeqiNP_598513.1. NM_133752.3. [P58281-1]
    XP_006522720.1. XM_006522657.1. [P58281-2]
    UniGeneiMm.274285.

    Genome annotation databases

    EnsembliENSMUST00000160597; ENSMUSP00000124223; ENSMUSG00000038084. [P58281-1]
    ENSMUST00000161186; ENSMUSP00000123880; ENSMUSG00000038084. [P58281-2]
    GeneIDi74143.
    KEGGimmu:74143.
    UCSCiuc007ywf.2. mouse. [P58281-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB044138 mRNA. Translation: BAB59000.1 .
    AK029157 mRNA. Translation: BAC26331.1 .
    AK038446 mRNA. Translation: BAC30002.1 .
    AK044657 mRNA. Translation: BAC32021.1 .
    AK050383 mRNA. Translation: BAC34224.1 .
    AK145620 mRNA. Translation: BAE26544.1 .
    BC025160 mRNA. Translation: AAH25160.1 . Different initiation.
    BC138665 mRNA. Translation: AAI38666.1 .
    BC145959 mRNA. Translation: AAI45960.1 .
    CCDSi CCDS28096.1. [P58281-1 ]
    RefSeqi NP_598513.1. NM_133752.3. [P58281-1 ]
    XP_006522720.1. XM_006522657.1. [P58281-2 ]
    UniGenei Mm.274285.

    3D structure databases

    ProteinModelPortali P58281.
    SMRi P58281. Positions 262-601.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P58281. 7 interactions.
    MINTi MINT-4105859.

    PTM databases

    PhosphoSitei P58281.

    Proteomic databases

    MaxQBi P58281.
    PaxDbi P58281.
    PRIDEi P58281.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000160597 ; ENSMUSP00000124223 ; ENSMUSG00000038084 . [P58281-1 ]
    ENSMUST00000161186 ; ENSMUSP00000123880 ; ENSMUSG00000038084 . [P58281-2 ]
    GeneIDi 74143.
    KEGGi mmu:74143.
    UCSCi uc007ywf.2. mouse. [P58281-1 ]

    Organism-specific databases

    CTDi 4976.
    MGIi MGI:1921393. Opa1.

    Phylogenomic databases

    eggNOGi COG0699.
    GeneTreei ENSGT00550000074851.
    HOVERGENi HBG019108.
    KOi K17079.
    OMAi KRHKWNE.
    OrthoDBi EOG7TMZR4.
    PhylomeDBi P58281.
    TreeFami TF314250.

    Miscellaneous databases

    ChiTaRSi OPA1. mouse.
    NextBioi 339890.
    PROi P58281.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P58281.
    Bgeei P58281.
    CleanExi MM_OPA1.
    Genevestigatori P58281.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR001401. Dynamin_GTPase.
    IPR022812. Dynamin_SF.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR11566. PTHR11566. 1 hit.
    Pfami PF00350. Dynamin_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00195. DYNAMIN.
    SMARTi SM00053. DYNc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS51718. G_DYNAMIN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of a dynamin-related mouse mitochondrial GTPase and its distribution in brain, subcellular localization, and effect on mitochondrial morphology."
      Misaka T., Miyashita T., Kubo Y.
      J. Biol. Chem. 277:15834-15842(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Brain.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-365 (ISOFORM 2).
      Strain: C57BL/6J.
      Tissue: Blastocyst, Hypothalamus, Liver, Retina and Skin.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: Czech II.
      Tissue: Brain and Mammary gland.
    4. "Mitochondrial rhomboid PARL regulates cytochrome c release during apoptosis via OPA1-dependent cristae remodeling."
      Cipolat S., Rudka T., Hartmann D., Costa V., Serneels L., Craessaerts K., Metzger K., Frezza C., Annaert W., D'Adamio L., Derks C., Dejaegere T., Pellegrini L., D'Hooge R., Scorrano L., De Strooper B.
      Cell 126:163-175(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH PARL, DISRUPTION PHENOTYPE.
    5. Cited for: FUNCTION, SUBUNIT.
    6. "Regulation of OPA1 processing and mitochondrial fusion by m-AAA protease isoenzymes and OMA1."
      Ehses S., Raschke I., Mancuso G., Bernacchia A., Geimer S., Tondera D., Martinou J.C., Westermann B., Rugarli E.I., Langer T.
      J. Cell Biol. 187:1023-1036(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION (DYNAMIN-LIKE 120 KDA PROTEIN; FORM S1), PROTEOLYTIC PROCESSING.
    7. "ChChd3, an inner mitochondrial membrane protein, is essential for maintaining crista integrity and mitochondrial function."
      Darshi M., Mendiola V.L., Mackey M.R., Murphy A.N., Koller A., Perkins G.A., Ellisman M.H., Taylor S.S.
      J. Biol. Chem. 286:2918-2932(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CHCHD3, IMMT.
    8. "Loss of mitochondrial protease OMA1 alters processing of the GTPase OPA1 and causes obesity and defective thermogenesis in mice."
      Quiros P.M., Ramsay A.J., Sala D., Fernandez-Vizarra E., Rodriguez F., Peinado J.R., Fernandez-Garcia M.S., Vega J.A., Enriquez J.A., Zorzano A., Lopez-Otin C.
      EMBO J. 31:2117-2133(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION (DYNAMIN-LIKE 120 KDA PROTEIN; FORM S1), PROTEOLYTIC PROCESSING.

    Entry informationi

    Entry nameiOPA1_MOUSE
    AccessioniPrimary (citable) accession number: P58281
    Secondary accession number(s): A6H6Q3
    , Q3ULA5, Q8BKU7, Q8BLL3, Q8BM08, Q8R3J7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: September 26, 2001
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3