P58185 (LPXK_RHILO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tetraacyldisaccharide 4'-kinase
EC=2.7.1.130

Alternative name(s):
Lipid A 4'-kinase

Gene names

Name:	lpxK
Ordered Locus Names:	mlr8270

Organism

Rhizobium loti (strain MAFF303099) (Mesorhizobium loti) [Complete proteome] [HAMAP]

Taxonomic identifier

266835 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Phyllobacteriaceae › Mesorhizobium

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA) By similarity. HAMAP MF_00409
Catalytic activity	ATP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-4-O-phosphono-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate). HAMAP MF_00409
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 6/6. HAMAP MF_00409
Sequence similarities	Belongs to the LpxK family.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tetraacyldisaccharide 4'-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Tetraacyldisaccharide 4'-kinase HAMAP MF_00409		PRO_0000190941
Regions
	Nucleotide binding	54 – 61	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P58185 [UniParc]. Last modified June 20, 2001. Version 1. Checksum: 296E739A338120F4	FASTA	341	36,941
	10 20 30 40 50 60 MASEAPPFWW EEPDWKVLAL SPLSAVYALV AGRVMRRARR EKIEAPVLCV GNFTVGGTGK 70 80 90 100 110 120 TPVAIALAQQ AKRMQLKPGF LSRGHGGSFA EPHVVDAHHD AAKHVGDEPL LLAEHAPVAV 130 140 150 160 170 180 TPNRAAGARL LMAKHGCDFL IMDDGFQSAR IHIDYALIVV DARYGIGNGR VIPGGPLRAK 190 200 210 220 230 240 IVDQLVFTSG LLKMGEGTAA DAVVRQAARA GRPIFEARAE PISKAGLAGK RFLAFAGIGH 250 260 270 280 290 300 PDKFFDTVRE AGGEVVLSKP FPDHHFYAED ELAELAAVAR AEGLGLITTA KDAARLRHGA 310 320 330 340 SQDFLNRLEV LEIDTVFELD HAPERIIEET LDAWRQRKLR S

References

[1]

"Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti." Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S., Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y., Nakayama S. , Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M., Tabata S. DNA Res. 7:331-338(2000) [PubMed: 11214968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MAFF303099.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000012 Genomic DNA. Translation: BAB53863.1.
RefSeq	NP_108402.1. NC_002678.2.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1231054.
GenomeReviews	Gene locus mlr8270 in contig BA000012_GR.
KEGG	mlo:mlr8270.
NMPDR	fig|266835.1.peg.6501.
PATRIC	22487469. VBIMesLot2464_6608.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG663914.
OMA	VILMDDG.
ProtClustDB	PRK00652.
Family and domain databases
HAMAP	MF_00409. LpxK. [Tree]
InterPro	IPR003758. Tetraacyldisaccharide_4-kinase. [Graphical view]
KO	K00912.
Pfam	PF02606. LpxK. 1 hit. [Graphical view]
TIGRFAMs	TIGR00682. LpxK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LPXK_RHILO

Accession

Primary (citable) accession number: P58185

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	June 20, 2001
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program