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P58158

- B3GA3_MOUSE

UniProt

P58158 - B3GA3_MOUSE

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Protein
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3
Gene
B3gat3
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Glycosaminoglycans biosynthesis. Involved in forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate. Transfers a glucuronic acid moiety from the uridine diphosphate-glucuronic acid (UDP-GlcUA) to the common linkage region trisaccharide Gal-beta-1,3-Gal-beta-1,4-Xyl covalently bound to a Ser residue at the glycosaminylglycan attachment site of proteoglycans. Can also play a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins By similarity.

Catalytic activityi

UDP-glucuronate + 3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein = UDP + 3-beta-D-glucuronosyl-3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein.

Cofactori

Manganese By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei113 – 1131UDP-glucuronate By similarity
Binding sitei156 – 1561UDP-glucuronate By similarity
Binding sitei161 – 1611UDP-glucuronate By similarity
Metal bindingi196 – 1961Manganese By similarity
Sitei227 – 2271Interaction with galactose moiety of substrate glycoprotein By similarity
Active sitei281 – 2811Proton donor/acceptor By similarity
Sitei318 – 3181Interaction with galactose moiety of substrate glycoprotein By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi82 – 843UDP-glucuronate binding By similarity
Nucleotide bindingi194 – 1963UDP-glucuronate binding By similarity
Nucleotide bindingi308 – 3103UDP-glucuronate binding By similarity

GO - Molecular functioni

  1. galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. chondroitin sulfate proteoglycan biosynthetic process Source: Ensembl
  2. dermatan sulfate proteoglycan biosynthetic process Source: Ensembl
  3. heparan sulfate proteoglycan biosynthetic process Source: MGI
  4. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_196489. A tetrasaccharide linker sequence is required for GAG synthesis.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT43. Glycosyltransferase Family 43.

Names & Taxonomyi

Protein namesi
Recommended name:
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3 (EC:2.4.1.135)
Alternative name(s):
Beta-1,3-glucuronyltransferase 3
Glucuronosyltransferase I
Short name:
GlcAT-I
UDP-GlcUA:Gal beta-1,3-Gal-R glucuronyltransferase
Short name:
GlcUAT-I
Gene namesi
Name:B3gat3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 19

Organism-specific databases

MGIiMGI:1919977. B3gat3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77Cytoplasmic Reviewed prediction
Transmembranei8 – 2821Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST
Topological domaini29 – 335307Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi membrane Source: UniProtKB-SubCell
  2. cis-Golgi network Source: Ensembl
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 335335Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3
PRO_0000195177Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi33 – 33Interchain By similarity
Glycosylationi300 – 3001N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

N-glycosylated By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP58158.
PRIDEiP58158.

PTM databases

PhosphoSiteiP58158.

Expressioni

Tissue specificityi

Expressed in heart, aorta, bone, and also in osteoblasts.1 Publication

Gene expression databases

BgeeiP58158.
GenevestigatoriP58158.

Interactioni

Subunit structurei

Homodimer; disulfide-linked By similarity.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000093962.

Structurei

3D structure databases

ProteinModelPortaliP58158.
SMRiP58158. Positions 76-335.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni243 – 25210Interaction with galactose moiety of substrate glycoprotein By similarity

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG310844.
GeneTreeiENSGT00390000017640.
HOGENOMiHOG000261693.
HOVERGENiHBG050650.
InParanoidiP58158.
KOiK10158.
OMAiPNAQFDS.
OrthoDBiEOG7QG44X.
PhylomeDBiP58158.
TreeFamiTF313522.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR005027. Glyco_trans_43.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR10896. PTHR10896. 1 hit.
PfamiPF03360. Glyco_transf_43. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

P58158-1 [UniParc]FASTAAdd to Basket

« Hide

MKLKLKNVFL AYFLVSIAGL LYALVQLGQP CDCLPPLRAA AEQLRQKDLR    50
ISQLQADLRR PPPVPAQPPE PEALPTIYVI TPTYARLVQK AELVRLSQTL 100
SLVPRLHWLL VEDAESPTPL VSGLLAASGL LFTHLAVLTP KAQRLREGEP 150
GWVRPRGVEQ RNKALDWLRG KGGAVGGEKD PPPPGTQGVV YFADDDNTYS 200
RELFKEMRWT RGVSVWPVGL VGGLRFEGPQ VQDGRVVGFH TAWEPNRPFP 250
LDMAGFAVAL PLLLAKPNAQ FDATAPRGHL ESSLLSHLVD PKDLEPRAAN 300
CTQVLVWHTR TEKPKMKQEE QLQRQGQGSD PAIEV 335
Length:335
Mass (Da):37,067
Last modified:June 1, 2001 - v1
Checksum:i906EED2AB672F1EC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC002103 mRNA. Translation: AAH02103.1.
BC004038 mRNA. Translation: AAH04038.1.
BC012930 mRNA. Translation: AAH12930.1.
CCDSiCCDS29558.1.
RefSeqiNP_077218.1. NM_024256.2.
UniGeneiMm.259.

Genome annotation databases

EnsembliENSMUST00000096243; ENSMUSP00000093962; ENSMUSG00000071649.
GeneIDi72727.
KEGGimmu:72727.
UCSCiuc008gob.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC002103 mRNA. Translation: AAH02103.1 .
BC004038 mRNA. Translation: AAH04038.1 .
BC012930 mRNA. Translation: AAH12930.1 .
CCDSi CCDS29558.1.
RefSeqi NP_077218.1. NM_024256.2.
UniGenei Mm.259.

3D structure databases

ProteinModelPortali P58158.
SMRi P58158. Positions 76-335.
ModBasei Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000093962.

Protein family/group databases

CAZyi GT43. Glycosyltransferase Family 43.

PTM databases

PhosphoSitei P58158.

Proteomic databases

PaxDbi P58158.
PRIDEi P58158.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000096243 ; ENSMUSP00000093962 ; ENSMUSG00000071649 .
GeneIDi 72727.
KEGGi mmu:72727.
UCSCi uc008gob.1. mouse.

Organism-specific databases

CTDi 26229.
MGIi MGI:1919977. B3gat3.

Phylogenomic databases

eggNOGi NOG310844.
GeneTreei ENSGT00390000017640.
HOGENOMi HOG000261693.
HOVERGENi HBG050650.
InParanoidi P58158.
KOi K10158.
OMAi PNAQFDS.
OrthoDBi EOG7QG44X.
PhylomeDBi P58158.
TreeFami TF313522.

Enzyme and pathway databases

UniPathwayi UPA00378 .
Reactomei REACT_196489. A tetrasaccharide linker sequence is required for GAG synthesis.

Miscellaneous databases

NextBioi 336810.
PROi P58158.
SOURCEi Search...

Gene expression databases

Bgeei P58158.
Genevestigatori P58158.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR005027. Glyco_trans_43.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
PANTHERi PTHR10896. PTHR10896. 1 hit.
Pfami PF03360. Glyco_transf_43. 1 hit.
[Graphical view ]
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Eye and Mammary tumor.
  2. Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiB3GA3_MOUSE
AccessioniPrimary (citable) accession number: P58158
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: June 1, 2001
Last modified: September 3, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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