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P58158

- B3GA3_MOUSE

UniProt

P58158 - B3GA3_MOUSE

Protein

Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3

Gene

B3gat3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Glycosaminoglycans biosynthesis. Involved in forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate. Transfers a glucuronic acid moiety from the uridine diphosphate-glucuronic acid (UDP-GlcUA) to the common linkage region trisaccharide Gal-beta-1,3-Gal-beta-1,4-Xyl covalently bound to a Ser residue at the glycosaminylglycan attachment site of proteoglycans. Can also play a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins By similarity.By similarity

    Catalytic activityi

    UDP-glucuronate + 3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein = UDP + 3-beta-D-glucuronosyl-3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein.

    Cofactori

    Manganese.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei113 – 1131UDP-glucuronateBy similarity
    Binding sitei156 – 1561UDP-glucuronateBy similarity
    Binding sitei161 – 1611UDP-glucuronateBy similarity
    Metal bindingi196 – 1961ManganeseBy similarity
    Sitei227 – 2271Interaction with galactose moiety of substrate glycoproteinBy similarity
    Active sitei281 – 2811Proton donor/acceptorBy similarity
    Sitei318 – 3181Interaction with galactose moiety of substrate glycoproteinBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi82 – 843UDP-glucuronate bindingBy similarity
    Nucleotide bindingi194 – 1963UDP-glucuronate bindingBy similarity
    Nucleotide bindingi308 – 3103UDP-glucuronate bindingBy similarity

    GO - Molecular functioni

    1. galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. chondroitin sulfate proteoglycan biosynthetic process Source: Ensembl
    2. dermatan sulfate proteoglycan biosynthetic process Source: Ensembl
    3. heparan sulfate proteoglycan biosynthetic process Source: MGI
    4. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_196489. A tetrasaccharide linker sequence is required for GAG synthesis.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT43. Glycosyltransferase Family 43.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3 (EC:2.4.1.135)
    Alternative name(s):
    Beta-1,3-glucuronyltransferase 3
    Glucuronosyltransferase I
    Short name:
    GlcAT-I
    UDP-GlcUA:Gal beta-1,3-Gal-R glucuronyltransferase
    Short name:
    GlcUAT-I
    Gene namesi
    Name:B3gat3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:1919977. B3gat3.

    Subcellular locationi

    GO - Cellular componenti

    1. cis-Golgi network Source: Ensembl
    2. Golgi membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 335335Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3PRO_0000195177Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi33 – 33InterchainBy similarity
    Glycosylationi300 – 3001N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    N-glycosylated.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiP58158.
    PRIDEiP58158.

    PTM databases

    PhosphoSiteiP58158.

    Expressioni

    Tissue specificityi

    Expressed in heart, aorta, bone, and also in osteoblasts.1 Publication

    Gene expression databases

    BgeeiP58158.
    GenevestigatoriP58158.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000093962.

    Structurei

    3D structure databases

    ProteinModelPortaliP58158.
    SMRiP58158. Positions 76-335.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 77CytoplasmicSequence Analysis
    Topological domaini29 – 335307LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei8 – 2821Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni243 – 25210Interaction with galactose moiety of substrate glycoproteinBy similarity

    Sequence similaritiesi

    Belongs to the glycosyltransferase 43 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG310844.
    GeneTreeiENSGT00390000017640.
    HOGENOMiHOG000261693.
    HOVERGENiHBG050650.
    InParanoidiP58158.
    KOiK10158.
    OMAiPNAQFDS.
    OrthoDBiEOG7QG44X.
    PhylomeDBiP58158.
    TreeFamiTF313522.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR005027. Glyco_trans_43.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR10896. PTHR10896. 1 hit.
    PfamiPF03360. Glyco_transf_43. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P58158-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLKLKNVFL AYFLVSIAGL LYALVQLGQP CDCLPPLRAA AEQLRQKDLR    50
    ISQLQADLRR PPPVPAQPPE PEALPTIYVI TPTYARLVQK AELVRLSQTL 100
    SLVPRLHWLL VEDAESPTPL VSGLLAASGL LFTHLAVLTP KAQRLREGEP 150
    GWVRPRGVEQ RNKALDWLRG KGGAVGGEKD PPPPGTQGVV YFADDDNTYS 200
    RELFKEMRWT RGVSVWPVGL VGGLRFEGPQ VQDGRVVGFH TAWEPNRPFP 250
    LDMAGFAVAL PLLLAKPNAQ FDATAPRGHL ESSLLSHLVD PKDLEPRAAN 300
    CTQVLVWHTR TEKPKMKQEE QLQRQGQGSD PAIEV 335
    Length:335
    Mass (Da):37,067
    Last modified:June 1, 2001 - v1
    Checksum:i906EED2AB672F1EC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC002103 mRNA. Translation: AAH02103.1.
    BC004038 mRNA. Translation: AAH04038.1.
    BC012930 mRNA. Translation: AAH12930.1.
    CCDSiCCDS29558.1.
    RefSeqiNP_077218.1. NM_024256.2.
    UniGeneiMm.259.

    Genome annotation databases

    EnsembliENSMUST00000096243; ENSMUSP00000093962; ENSMUSG00000071649.
    GeneIDi72727.
    KEGGimmu:72727.
    UCSCiuc008gob.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC002103 mRNA. Translation: AAH02103.1 .
    BC004038 mRNA. Translation: AAH04038.1 .
    BC012930 mRNA. Translation: AAH12930.1 .
    CCDSi CCDS29558.1.
    RefSeqi NP_077218.1. NM_024256.2.
    UniGenei Mm.259.

    3D structure databases

    ProteinModelPortali P58158.
    SMRi P58158. Positions 76-335.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000093962.

    Protein family/group databases

    CAZyi GT43. Glycosyltransferase Family 43.

    PTM databases

    PhosphoSitei P58158.

    Proteomic databases

    PaxDbi P58158.
    PRIDEi P58158.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000096243 ; ENSMUSP00000093962 ; ENSMUSG00000071649 .
    GeneIDi 72727.
    KEGGi mmu:72727.
    UCSCi uc008gob.1. mouse.

    Organism-specific databases

    CTDi 26229.
    MGIi MGI:1919977. B3gat3.

    Phylogenomic databases

    eggNOGi NOG310844.
    GeneTreei ENSGT00390000017640.
    HOGENOMi HOG000261693.
    HOVERGENi HBG050650.
    InParanoidi P58158.
    KOi K10158.
    OMAi PNAQFDS.
    OrthoDBi EOG7QG44X.
    PhylomeDBi P58158.
    TreeFami TF313522.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    Reactomei REACT_196489. A tetrasaccharide linker sequence is required for GAG synthesis.

    Miscellaneous databases

    NextBioi 336810.
    PROi P58158.
    SOURCEi Search...

    Gene expression databases

    Bgeei P58158.
    Genevestigatori P58158.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR005027. Glyco_trans_43.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    PANTHERi PTHR10896. PTHR10896. 1 hit.
    Pfami PF03360. Glyco_transf_43. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Eye and Mammary tumor.
    2. Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiB3GA3_MOUSE
    AccessioniPrimary (citable) accession number: P58158
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 2001
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 124 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3