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Protein

Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3

Gene

B3gat3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Glycosaminoglycans biosynthesis. Involved in forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate. Transfers a glucuronic acid moiety from the uridine diphosphate-glucuronic acid (UDP-GlcUA) to the common linkage region trisaccharide Gal-beta-1,3-Gal-beta-1,4-Xyl covalently bound to a Ser residue at the glycosaminylglycan attachment site of proteoglycans. Can also play a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins. Stimulates 2-phosphoxylose phosphatase activity of PXYLP1 in presence of uridine diphosphate-glucuronic acid (UDP-GlcUA) during completion of linkage region formation.By similarity

Catalytic activityi

UDP-glucuronate + 3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein = UDP + 3-beta-D-glucuronosyl-3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein.

Cofactori

Mn2+By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei113 – 1131UDP-glucuronateBy similarity
Binding sitei156 – 1561UDP-glucuronateBy similarity
Binding sitei161 – 1611UDP-glucuronateBy similarity
Metal bindingi196 – 1961ManganeseBy similarity
Sitei227 – 2271Interaction with galactose moiety of substrate glycoproteinBy similarity
Active sitei281 – 2811Proton donor/acceptorBy similarity
Sitei318 – 3181Interaction with galactose moiety of substrate glycoproteinBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi82 – 843UDP-glucuronate bindingBy similarity
Nucleotide bindingi194 – 1963UDP-glucuronate bindingBy similarity
Nucleotide bindingi308 – 3103UDP-glucuronate bindingBy similarity

GO - Molecular functioni

  1. galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity Source: UniProtKB-EC
  2. glucuronosyltransferase activity Source: MGI
  3. metal ion binding Source: UniProtKB-KW
  4. protein phosphatase activator activity Source: UniProtKB

GO - Biological processi

  1. chondroitin sulfate proteoglycan biosynthetic process Source: MGI
  2. dermatan sulfate proteoglycan biosynthetic process Source: MGI
  3. glycosaminoglycan biosynthetic process Source: UniProtKB
  4. heparan sulfate proteoglycan biosynthetic process Source: MGI
  5. positive regulation of catalytic activity Source: UniProtKB
  6. positive regulation of intracellular protein transport Source: UniProtKB
  7. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_326072. A tetrasaccharide linker sequence is required for GAG synthesis.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT43. Glycosyltransferase Family 43.

Names & Taxonomyi

Protein namesi
Recommended name:
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3 (EC:2.4.1.135)
Alternative name(s):
Beta-1,3-glucuronyltransferase 3
Glucuronosyltransferase I
Short name:
GlcAT-I
UDP-GlcUA:Gal beta-1,3-Gal-R glucuronyltransferase
Short name:
GlcUAT-I
Gene namesi
Name:B3gat3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1919977. B3gat3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77CytoplasmicSequence Analysis
Transmembranei8 – 2821Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini29 – 335307LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cis-Golgi network Source: MGI
  2. extracellular vesicular exosome Source: MGI
  3. Golgi apparatus Source: MGI
  4. Golgi membrane Source: UniProtKB-SubCell
  5. integral component of membrane Source: UniProtKB-KW
  6. membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 335335Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3PRO_0000195177Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi33 – 33InterchainBy similarity
Glycosylationi300 – 3001N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP58158.
PaxDbiP58158.
PRIDEiP58158.

PTM databases

PhosphoSiteiP58158.

Expressioni

Tissue specificityi

Expressed in heart, aorta, bone, and also in osteoblasts.1 Publication

Gene expression databases

BgeeiP58158.
GenevestigatoriP58158.

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Interacts with PXYLP1; the interaction increases the 2-phosphoxylose phosphatase activity of PXYLP1 during completion of linkage region formation in a B3GAT3-mediated manner.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000093962.

Structurei

3D structure databases

ProteinModelPortaliP58158.
SMRiP58158. Positions 76-335.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni243 – 25210Interaction with galactose moiety of substrate glycoproteinBy similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 43 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG310844.
GeneTreeiENSGT00390000017640.
HOGENOMiHOG000261693.
HOVERGENiHBG050650.
InParanoidiP58158.
KOiK10158.
OMAiRAANCTQ.
OrthoDBiEOG7QG44X.
PhylomeDBiP58158.
TreeFamiTF313522.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR005027. Glyco_trans_43.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR10896. PTHR10896. 1 hit.
PfamiPF03360. Glyco_transf_43. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

P58158-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLKLKNVFL AYFLVSIAGL LYALVQLGQP CDCLPPLRAA AEQLRQKDLR
60 70 80 90 100
ISQLQADLRR PPPVPAQPPE PEALPTIYVI TPTYARLVQK AELVRLSQTL
110 120 130 140 150
SLVPRLHWLL VEDAESPTPL VSGLLAASGL LFTHLAVLTP KAQRLREGEP
160 170 180 190 200
GWVRPRGVEQ RNKALDWLRG KGGAVGGEKD PPPPGTQGVV YFADDDNTYS
210 220 230 240 250
RELFKEMRWT RGVSVWPVGL VGGLRFEGPQ VQDGRVVGFH TAWEPNRPFP
260 270 280 290 300
LDMAGFAVAL PLLLAKPNAQ FDATAPRGHL ESSLLSHLVD PKDLEPRAAN
310 320 330
CTQVLVWHTR TEKPKMKQEE QLQRQGQGSD PAIEV
Length:335
Mass (Da):37,067
Last modified:June 1, 2001 - v1
Checksum:i906EED2AB672F1EC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC002103 mRNA. Translation: AAH02103.1.
BC004038 mRNA. Translation: AAH04038.1.
BC012930 mRNA. Translation: AAH12930.1.
CCDSiCCDS29558.1.
RefSeqiNP_077218.1. NM_024256.2.
UniGeneiMm.259.

Genome annotation databases

EnsembliENSMUST00000096243; ENSMUSP00000093962; ENSMUSG00000071649.
GeneIDi72727.
KEGGimmu:72727.
UCSCiuc008gob.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC002103 mRNA. Translation: AAH02103.1.
BC004038 mRNA. Translation: AAH04038.1.
BC012930 mRNA. Translation: AAH12930.1.
CCDSiCCDS29558.1.
RefSeqiNP_077218.1. NM_024256.2.
UniGeneiMm.259.

3D structure databases

ProteinModelPortaliP58158.
SMRiP58158. Positions 76-335.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000093962.

Protein family/group databases

CAZyiGT43. Glycosyltransferase Family 43.

PTM databases

PhosphoSiteiP58158.

Proteomic databases

MaxQBiP58158.
PaxDbiP58158.
PRIDEiP58158.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000096243; ENSMUSP00000093962; ENSMUSG00000071649.
GeneIDi72727.
KEGGimmu:72727.
UCSCiuc008gob.1. mouse.

Organism-specific databases

CTDi26229.
MGIiMGI:1919977. B3gat3.

Phylogenomic databases

eggNOGiNOG310844.
GeneTreeiENSGT00390000017640.
HOGENOMiHOG000261693.
HOVERGENiHBG050650.
InParanoidiP58158.
KOiK10158.
OMAiRAANCTQ.
OrthoDBiEOG7QG44X.
PhylomeDBiP58158.
TreeFamiTF313522.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiREACT_326072. A tetrasaccharide linker sequence is required for GAG synthesis.

Miscellaneous databases

NextBioi336810.
PROiP58158.
SOURCEiSearch...

Gene expression databases

BgeeiP58158.
GenevestigatoriP58158.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR005027. Glyco_trans_43.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR10896. PTHR10896. 1 hit.
PfamiPF03360. Glyco_transf_43. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Eye and Mammary tumor.
  2. Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiB3GA3_MOUSE
AccessioniPrimary (citable) accession number: P58158
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: June 1, 2001
Last modified: April 1, 2015
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.