Reviewed,
UniProtKB/Swiss-Prot P58158 (B3GA3_MOUSE)
Last modified
June 16, 2009.
Version 79.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3 EC=2.4.1.135 Alternative name(s): Beta-1,3-glucuronyltransferase 3 Glucuronosyltransferase-I Short name=GlcAT-I UDP-GlcUA:Gal beta-1,3-Gal-R glucuronyltransferase Short name=GlcUAT-I | ||
| Gene names |
| ||
| Organism | Mus musculus (Mouse) | ||
| Taxonomic identifier | 10090 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 335 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Glycosaminoglycans biosynthesis. Involved in forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate. Transfers a glucuronic acid moiety from the uridine diphosphate-glucuronic acid (UDP-GlcUA) to the common linkage region trisaccharide Gal-beta-1,3-Gal-beta-1,4-Xyl covalently bound to a Ser residue at the glycosaminylglycan attachment site of proteoglycans. Can also play a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins By similarity. |
| Catalytic activity | UDP-glucuronate + 3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein = UDP + 3-beta-D-glucuronosyl-3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein. |
| Cofactor | Manganese By similarity. |
| Pathway | |
| Subunit structure | Homodimer; disulfide-linked By similarity. |
| Subcellular location | Golgi apparatus membrane; Single-pass type II membrane protein By similarity. |
| Post-translational modification | N-glycosylated By similarity. |
| Sequence similarities | Belongs to the glycosyltransferase 43 family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Golgi apparatus Membrane |
| Domain | Signal-anchor Transmembrane |
| Ligand | Manganese Metal-binding |
| Molecular function | Transferase |
| PTM | Disulfide bond Glycoprotein |
| Gene Ontology (GO) | |
| Cellular component | Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membraneInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity Inferred from electronic annotation. Source: EC manganese ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 335 | 335 | Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3 | PRO_0000195177 | |||||
Regions | |||||||||
| Topological domain | 1 – 7 | 7 | Cytoplasmic Potential | ||||||
| Transmembrane | 8 – 28 | 21 | Signal-anchor for type II membrane protein Potential | ||||||
| Topological domain | 29 – 335 | 307 | Lumenal Potential | ||||||
Sites | |||||||||
| Active site | 281 | 1 | Proton acceptor By similarity | ||||||
| Metal binding | 196 | 1 | Manganese By similarity | ||||||
Amino acid modifications | |||||||||
| Glycosylation | 300 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Disulfide bond | 33 | Interchain By similarity | |||||||
Sequences
| ||||||||||||||||||
References
| [1] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Eye and Mammary tumor. |
Cross-references
Sequence databases | |
|---|---|
| BC002103 mRNA. Translation: AAH02103.1. BC004038 mRNA. Translation: AAH04038.1. BC012930 mRNA. Translation: AAH12930.1. | |
| IPI | IPI00116744. |
| RefSeq | NP_077218.1. |
| UniGene | Mm.259 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1KWS based on UniProtKB O94766. |
| SMR | P58158. Positions 76-335. |
| ModBase | Search... |
Protein family/group databases | |
| CAZy | GT43. Glycosyltransferase Family 43. |
PTM databases | |
| PhosphoSite | P58158. |
Proteomic databases | |
| PRIDE | P58158. |
Genome annotation databases | |
| Ensembl | ENSMUSG00000071649. Mus musculus. [Contig view] |
| GeneID | 72727. |
| KEGG | mmu:72727. |
Organism-specific databases | |
| MGI | MGI:1919977. B3gat3. |
Phylogenomic databases | |
| HOGENOM | P58158. |
| HOVERGEN | P58158. |
| OMA | P58158. REGEPGW. |
Enzyme and pathway databases | |
| BRENDA | 2.4.1.135. 244. |
Gene expression databases | |
| Bgee | P58158. |
| GermOnline | ENSMUSG00000071649. Mus musculus. |
Family and domain databases | |
| InterPro | IPR005027. Glyco_trans_43. [Graphical view] |
| PANTHER | PTHR10896. Glyco_trans_43. 1 hit. |
| Pfam | PF03360. Glyco_transf_43. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 336810. |
| SOURCE | Search... |
Entry information
| Entry name | B3GA3_MOUSE | ||||||||
| Accession | Primary (citable) accession number: P58158 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


