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Protein

Glycerol-3-phosphate acyltransferase

Gene

plsB

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.

Pathwayi: CDP-diacylglycerol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glycerol-3-phosphate acyltransferase (plsB)
  2. no protein annotated in this organism
  3. Phosphatidate cytidylyltransferase (cdsA), Phosphatidate cytidylyltransferase (ECs2011)
This subpathway is part of the pathway CDP-diacylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00557; UER00612.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate acyltransferase (EC:2.3.1.15)
Short name:
GPAT
Gene namesi
Name:plsB
Ordered Locus Names:Z5640, ECs5024
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001952202 – 807Glycerol-3-phosphate acyltransferaseAdd BLAST806

Proteomic databases

PRIDEiP58130.

Interactioni

Protein-protein interaction databases

STRINGi155864.Z5640.

Structurei

3D structure databases

ProteinModelPortaliP58130.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi306 – 311HXXXXD motif6

Domaini

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.By similarity

Sequence similaritiesi

Belongs to the GPAT/DAPAT family.Curated

Phylogenomic databases

eggNOGiENOG4105E55. Bacteria.
COG2937. LUCA.
HOGENOMiHOG000218231.
KOiK00631.
OMAiEVIYVPC.

Family and domain databases

HAMAPiMF_00393. Glyc3P_acyltrans. 1 hit.
InterProiIPR022284. GPAT/DHAPAT.
IPR028354. GPAT_PlsB.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PANTHERiPTHR12563. PTHR12563. 2 hits.
PfamiPF01553. Acyltransferase. 1 hit.
[Graphical view]
PIRSFiPIRSF500064. GPAT. 1 hit.
PIRSF000437. GPAT_DHAPAT. 1 hit.
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03703. plsB. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P58130-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGWPRIYYK LLNLPLSILV KSKSIPADPA PELGLDTSRP IMYVLPYNSK
60 70 80 90 100
ADLLTLRAQC LAHDLPDPLE PLEIDGTLLP RYVFIHGGPR VFTYYTPKEE
110 120 130 140 150
SIKLFHDYLD LHRSNPNLDV QMVPVSVMFG RAPGREKGEV NPPLRMLNGV
160 170 180 190 200
QKFFAVLWLG RDSFVRFSPS VSLRRMADEH GTDKTIAQKL ARVARMHFAR
210 220 230 240 250
QRLAAVGPRL PARQDLFNKL LASRAIAKAV EDEARSKKIS HEKAQQNAIA
260 270 280 290 300
LMEEIAANFS YEMIRLTDRI LGFTWNRLYQ GINVHNAERV RQLAHDGHEL
310 320 330 340 350
VYVPCHRSHM DYLLLSYVLY HQGLVPPHIA AGINLNFWPA GPIFRRLGAF
360 370 380 390 400
FIRRTFKGNK LYSTVFREYL GELFSRGYSV EYFVEGGRSR TGRLLDPKTG
410 420 430 440 450
TLSMTIQAML RGGTRPITLI PIYIGYEHVM EVGTYAKELR GATKEKESLP
460 470 480 490 500
QMLRGLSKLR NLGQGYVNFG EPMPLMTYLN QHVPDWRESI DPIEAVRPAW
510 520 530 540 550
LTPTVNNIAA DLMVRINNAG AANAMNLCCT ALLASRQRSL TREQLTEQLN
560 570 580 590 600
CYLDLMRNVP YSTDSTVPSA SASELIDHAL QMNKFEVEKD TIGDIIILPR
610 620 630 640 650
EQAVLMTYYR NNIAHMLVLP SLMAAIVTQH RHISRDVLME HVNVLYPMLK
660 670 680 690 700
AELFLRWDRD ELPDVIDALA NEMQRQGLIT LQDDELHINP VHSRTLQLLA
710 720 730 740 750
AGARETLQRY AITFWLLSAN PSINRGTLEK ESRTVAQRLS VLHGINAPEF
760 770 780 790 800
FDKAVFSSLV LTLRDEGYIS DSGDAEPAET MKVYQLLAEL ITSDVRLTIE

SATQGEG
Length:807
Mass (Da):91,409
Last modified:January 23, 2007 - v2
Checksum:i26FC7F929639F593
GO

Sequence cautioni

The sequence AAG59240 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB38447 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG59240.1. Different initiation.
BA000007 Genomic DNA. Translation: BAB38447.1. Different initiation.
PIRiD86097.
H91256.
RefSeqiNP_313051.2. NC_002695.1.
WP_001301502.1. NZ_LPWC02000002.1.

Genome annotation databases

EnsemblBacteriaiAAG59240; AAG59240; Z5640.
BAB38447; BAB38447; BAB38447.
GeneIDi915055.
KEGGiece:Z5640.
ecs:ECs5024.
PATRICi18359643. VBIEscCol44059_4958.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG59240.1. Different initiation.
BA000007 Genomic DNA. Translation: BAB38447.1. Different initiation.
PIRiD86097.
H91256.
RefSeqiNP_313051.2. NC_002695.1.
WP_001301502.1. NZ_LPWC02000002.1.

3D structure databases

ProteinModelPortaliP58130.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi155864.Z5640.

Proteomic databases

PRIDEiP58130.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG59240; AAG59240; Z5640.
BAB38447; BAB38447; BAB38447.
GeneIDi915055.
KEGGiece:Z5640.
ecs:ECs5024.
PATRICi18359643. VBIEscCol44059_4958.

Phylogenomic databases

eggNOGiENOG4105E55. Bacteria.
COG2937. LUCA.
HOGENOMiHOG000218231.
KOiK00631.
OMAiEVIYVPC.

Enzyme and pathway databases

UniPathwayiUPA00557; UER00612.

Family and domain databases

HAMAPiMF_00393. Glyc3P_acyltrans. 1 hit.
InterProiIPR022284. GPAT/DHAPAT.
IPR028354. GPAT_PlsB.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PANTHERiPTHR12563. PTHR12563. 2 hits.
PfamiPF01553. Acyltransferase. 1 hit.
[Graphical view]
PIRSFiPIRSF500064. GPAT. 1 hit.
PIRSF000437. GPAT_DHAPAT. 1 hit.
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03703. plsB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPLSB_ECO57
AccessioniPrimary (citable) accession number: P58130
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.