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Protein

RNA 3'-terminal phosphate cyclase

Gene

rtcA

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing (By similarity).By similarity

Catalytic activityi

ATP + (RNA)-3'-(3'-phospho-ribonucleoside) = AMP + diphosphate + (RNA)-3'-(2',3'-cyclophospho)-ribonucleoside.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei103ATPBy similarity1
Active sitei308Tele-AMP-histidine intermediateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi283 – 287ATPBy similarity5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 3'-terminal phosphate cyclase (EC:6.5.1.4)
Short name:
RNA cyclase
Short name:
RNA-3'-phosphate cyclase
Gene namesi
Name:rtcA
Ordered Locus Names:Z4778, ECs4263
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001564181 – 342RNA 3'-terminal phosphate cyclaseAdd BLAST342

Interactioni

Protein-protein interaction databases

STRINGi155864.Z4778

Structurei

3D structure databases

ProteinModelPortaliP58127
SMRiP58127
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CKX Bacteria
COG0430 LUCA
HOGENOMiHOG000015264
KOiK01974
OMAiANRPKPG

Family and domain databases

Gene3Di3.30.360.20, 1 hit
3.65.10.20, 2 hits
HAMAPiMF_00200 RTC, 1 hit
InterProiView protein in InterPro
IPR013791 RNA3'-term_phos_cycl_insert
IPR023797 RNA3'_phos_cyclase_dom
IPR037136 RNA3'_phos_cyclase_dom_sf
IPR000228 RNA3'_term_phos_cyc
IPR017770 RNA3'_term_phos_cyc_type_1
IPR020719 RNA3'_term_phos_cycl-like_CS
IPR013792 RNA3'P_cycl/enolpyr_Trfase_a/b
IPR036553 RPTC_insert
PANTHERiPTHR11096 PTHR11096, 1 hit
PTHR11096:SF0 PTHR11096:SF0, 1 hit
PfamiView protein in Pfam
PF01137 RTC, 1 hit
PF05189 RTC_insert, 1 hit
PIRSFiPIRSF005378 RNA3'_term_phos_cycl_euk, 1 hit
SUPFAMiSSF52913 SSF52913, 1 hit
SSF55205 SSF55205, 2 hits
TIGRFAMsiTIGR03399 RNA_3prim_cycl, 1 hit
PROSITEiView protein in PROSITE
PS01287 RTC, 1 hit

Sequencei

Sequence statusi: Complete.

P58127-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRMIALDGA QGEGGGQIMR SALSLSMITG QPFTITGIRA GRAKPGLLRQ
60 70 80 90 100
HLTAVKAATE ICGATVEGAE LGSQRLVFRP GTVRGGDYRF AIGSAGSCTL
110 120 130 140 150
VLQTVLPALW FADGPSRVEV SGGTDNPSAP PADFIRRVLE PLLAKIGIHQ
160 170 180 190 200
QTTLLRHGFY PAGGGVVATE VSPVASFNTL QLGERGNIVQ MRGEVLLAGV
210 220 230 240 250
PRHVAEREIA TLAASFSLHE QNIHNLPRDQ GPGNTVSLEV ESENITERFF
260 270 280 290 300
VVGEKRVSAE VVAAQLVKEV KRYLASPAAV GEYLADQLVL PMALAGAGQF
310 320 330 340
TVAHPSCHLL TNIAVVERFL PVRFTLAETD GVTRVMITKL TD
Length:342
Mass (Da):36,332
Last modified:June 1, 2001 - v1
Checksum:i783FE7FAD7160846
GO

Sequence cautioni

The sequence BAB37686 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA Translation: AAG58524.1
BA000007 Genomic DNA Translation: BAB37686.1 Different initiation.
PIRiG91161
H86007
RefSeqiNP_312290.2, NC_002695.1
WP_000827117.1, NZ_NOKN01000002.1

Genome annotation databases

EnsemblBacteriaiAAG58524; AAG58524; Z4778
BAB37686; BAB37686; BAB37686
GeneIDi915880
KEGGiece:Z4778
ecs:ECs4263
PATRICifig|386585.9.peg.4453

Similar proteinsi

Entry informationi

Entry nameiRTCA_ECO57
AccessioniPrimary (citable) accession number: P58127
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: June 1, 2001
Last modified: April 25, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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