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P58127

- RTCA_ECO57

UniProt

P58127 - RTCA_ECO57

Protein

RNA 3'-terminal phosphate cyclase

Gene

rtcA

Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity.By similarity

    Catalytic activityi

    ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei103 – 1031ATPBy similarity
    Active sitei308 – 3081Tele-AMP-histidine intermediateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi283 – 2875ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. RNA-3'-phosphate cyclase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Ligase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciECOL386585:GJFA-4233-MONOMER.
    ECOO157:Z4778-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA 3'-terminal phosphate cyclase (EC:6.5.1.4)
    Short name:
    RNA cyclase
    Short name:
    RNA-3'-phosphate cyclase
    Gene namesi
    Name:rtcA
    Ordered Locus Names:Z4778, ECs4263
    OrganismiEscherichia coli O157:H7
    Taxonomic identifieri83334 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 342342RNA 3'-terminal phosphate cyclasePRO_0000156418Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi155864.Z4778.

    Structurei

    3D structure databases

    ProteinModelPortaliP58127.
    SMRiP58127. Positions 4-337.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0430.
    HOGENOMiHOG000015264.
    KOiK01974.
    OMAiAEMLLRN.
    OrthoDBiEOG6RNQDX.

    Family and domain databases

    Gene3Di3.30.360.20. 1 hit.
    3.65.10.20. 2 hits.
    HAMAPiMF_00200. RTC.
    InterProiIPR013791. RNA3'-term_phos_cycl_insert.
    IPR023797. RNA3'_phos_cyclase_dom.
    IPR000228. RNA3'_term_phos_cyc.
    IPR017770. RNA3'_term_phos_cyc_type_1.
    IPR020719. RNA3'_term_phos_cycl-like_CS.
    IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
    [Graphical view]
    PANTHERiPTHR11096. PTHR11096. 1 hit.
    PfamiPF01137. RTC. 1 hit.
    PF05189. RTC_insert. 1 hit.
    [Graphical view]
    SUPFAMiSSF52913. SSF52913. 1 hit.
    SSF55205. SSF55205. 2 hits.
    TIGRFAMsiTIGR03399. RNA_3prim_cycl. 1 hit.
    PROSITEiPS01287. RTC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P58127-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKRMIALDGA QGEGGGQIMR SALSLSMITG QPFTITGIRA GRAKPGLLRQ    50
    HLTAVKAATE ICGATVEGAE LGSQRLVFRP GTVRGGDYRF AIGSAGSCTL 100
    VLQTVLPALW FADGPSRVEV SGGTDNPSAP PADFIRRVLE PLLAKIGIHQ 150
    QTTLLRHGFY PAGGGVVATE VSPVASFNTL QLGERGNIVQ MRGEVLLAGV 200
    PRHVAEREIA TLAASFSLHE QNIHNLPRDQ GPGNTVSLEV ESENITERFF 250
    VVGEKRVSAE VVAAQLVKEV KRYLASPAAV GEYLADQLVL PMALAGAGQF 300
    TVAHPSCHLL TNIAVVERFL PVRFTLAETD GVTRVMITKL TD 342
    Length:342
    Mass (Da):36,332
    Last modified:June 1, 2001 - v1
    Checksum:i783FE7FAD7160846
    GO

    Sequence cautioni

    The sequence BAB37686.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005174 Genomic DNA. Translation: AAG58524.1.
    BA000007 Genomic DNA. Translation: BAB37686.1. Different initiation.
    PIRiG91161.
    H86007.
    RefSeqiNP_289963.1. NC_002655.2.
    NP_312290.1. NC_002695.1.

    Genome annotation databases

    EnsemblBacteriaiAAG58524; AAG58524; Z4778.
    BAB37686; BAB37686; BAB37686.
    GeneIDi915880.
    958908.
    KEGGiece:Z4778.
    ecs:ECs4263.
    PATRICi18358089. VBIEscCol44059_4211.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005174 Genomic DNA. Translation: AAG58524.1 .
    BA000007 Genomic DNA. Translation: BAB37686.1 . Different initiation.
    PIRi G91161.
    H86007.
    RefSeqi NP_289963.1. NC_002655.2.
    NP_312290.1. NC_002695.1.

    3D structure databases

    ProteinModelPortali P58127.
    SMRi P58127. Positions 4-337.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 155864.Z4778.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG58524 ; AAG58524 ; Z4778 .
    BAB37686 ; BAB37686 ; BAB37686 .
    GeneIDi 915880.
    958908.
    KEGGi ece:Z4778.
    ecs:ECs4263.
    PATRICi 18358089. VBIEscCol44059_4211.

    Phylogenomic databases

    eggNOGi COG0430.
    HOGENOMi HOG000015264.
    KOi K01974.
    OMAi AEMLLRN.
    OrthoDBi EOG6RNQDX.

    Enzyme and pathway databases

    BioCyci ECOL386585:GJFA-4233-MONOMER.
    ECOO157:Z4778-MONOMER.

    Family and domain databases

    Gene3Di 3.30.360.20. 1 hit.
    3.65.10.20. 2 hits.
    HAMAPi MF_00200. RTC.
    InterProi IPR013791. RNA3'-term_phos_cycl_insert.
    IPR023797. RNA3'_phos_cyclase_dom.
    IPR000228. RNA3'_term_phos_cyc.
    IPR017770. RNA3'_term_phos_cyc_type_1.
    IPR020719. RNA3'_term_phos_cycl-like_CS.
    IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
    [Graphical view ]
    PANTHERi PTHR11096. PTHR11096. 1 hit.
    Pfami PF01137. RTC. 1 hit.
    PF05189. RTC_insert. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52913. SSF52913. 1 hit.
    SSF55205. SSF55205. 2 hits.
    TIGRFAMsi TIGR03399. RNA_3prim_cycl. 1 hit.
    PROSITEi PS01287. RTC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
    2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
      Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
      , Kuhara S., Shiba T., Hattori M., Shinagawa H.
      DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

    Entry informationi

    Entry nameiRTCA_ECO57
    AccessioniPrimary (citable) accession number: P58127
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 2001
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3