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P58127

- RTCA_ECO57

UniProt

P58127 - RTCA_ECO57

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Protein

RNA 3'-terminal phosphate cyclase

Gene
rtcA, Z4778, ECs4263
Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity.UniRule annotation

Catalytic activityi

ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei103 – 1031ATP By similarity
Active sitei308 – 3081Tele-AMP-histidine intermediate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi283 – 2875ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA-3'-phosphate cyclase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciECOL386585:GJFA-4233-MONOMER.
ECOO157:Z4778-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 3'-terminal phosphate cyclase (EC:6.5.1.4)
Short name:
RNA cyclase
Short name:
RNA-3'-phosphate cyclase
Gene namesi
Name:rtcA
Ordered Locus Names:Z4778, ECs4263
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 342342RNA 3'-terminal phosphate cyclaseUniRule annotationPRO_0000156418Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi155864.Z4778.

Structurei

3D structure databases

ProteinModelPortaliP58127.
SMRiP58127. Positions 4-337.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0430.
HOGENOMiHOG000015264.
KOiK01974.
OMAiAEMLLRN.
OrthoDBiEOG6RNQDX.

Family and domain databases

Gene3Di3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
HAMAPiMF_00200. RTC.
InterProiIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERiPTHR11096. PTHR11096. 1 hit.
PfamiPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
SUPFAMiSSF52913. SSF52913. 1 hit.
SSF55205. SSF55205. 2 hits.
TIGRFAMsiTIGR03399. RNA_3prim_cycl. 1 hit.
PROSITEiPS01287. RTC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P58127-1 [UniParc]FASTAAdd to Basket

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MKRMIALDGA QGEGGGQIMR SALSLSMITG QPFTITGIRA GRAKPGLLRQ    50
HLTAVKAATE ICGATVEGAE LGSQRLVFRP GTVRGGDYRF AIGSAGSCTL 100
VLQTVLPALW FADGPSRVEV SGGTDNPSAP PADFIRRVLE PLLAKIGIHQ 150
QTTLLRHGFY PAGGGVVATE VSPVASFNTL QLGERGNIVQ MRGEVLLAGV 200
PRHVAEREIA TLAASFSLHE QNIHNLPRDQ GPGNTVSLEV ESENITERFF 250
VVGEKRVSAE VVAAQLVKEV KRYLASPAAV GEYLADQLVL PMALAGAGQF 300
TVAHPSCHLL TNIAVVERFL PVRFTLAETD GVTRVMITKL TD 342
Length:342
Mass (Da):36,332
Last modified:June 1, 2001 - v1
Checksum:i783FE7FAD7160846
GO

Sequence cautioni

The sequence BAB37686.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005174 Genomic DNA. Translation: AAG58524.1.
BA000007 Genomic DNA. Translation: BAB37686.1. Different initiation.
PIRiG91161.
H86007.
RefSeqiNP_289963.1. NC_002655.2.
NP_312290.1. NC_002695.1.

Genome annotation databases

EnsemblBacteriaiAAG58524; AAG58524; Z4778.
BAB37686; BAB37686; BAB37686.
GeneIDi915880.
958908.
KEGGiece:Z4778.
ecs:ECs4263.
PATRICi18358089. VBIEscCol44059_4211.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005174 Genomic DNA. Translation: AAG58524.1 .
BA000007 Genomic DNA. Translation: BAB37686.1 . Different initiation.
PIRi G91161.
H86007.
RefSeqi NP_289963.1. NC_002655.2.
NP_312290.1. NC_002695.1.

3D structure databases

ProteinModelPortali P58127.
SMRi P58127. Positions 4-337.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 155864.Z4778.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG58524 ; AAG58524 ; Z4778 .
BAB37686 ; BAB37686 ; BAB37686 .
GeneIDi 915880.
958908.
KEGGi ece:Z4778.
ecs:ECs4263.
PATRICi 18358089. VBIEscCol44059_4211.

Phylogenomic databases

eggNOGi COG0430.
HOGENOMi HOG000015264.
KOi K01974.
OMAi AEMLLRN.
OrthoDBi EOG6RNQDX.

Enzyme and pathway databases

BioCyci ECOL386585:GJFA-4233-MONOMER.
ECOO157:Z4778-MONOMER.

Family and domain databases

Gene3Di 3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
HAMAPi MF_00200. RTC.
InterProi IPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view ]
PANTHERi PTHR11096. PTHR11096. 1 hit.
Pfami PF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view ]
SUPFAMi SSF52913. SSF52913. 1 hit.
SSF55205. SSF55205. 2 hits.
TIGRFAMsi TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITEi PS01287. RTC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Entry informationi

Entry nameiRTCA_ECO57
AccessioniPrimary (citable) accession number: P58127
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: June 1, 2001
Last modified: May 14, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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