Reviewed, UniProtKB/Swiss-Prot P58072 (G3P3_ECO57)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase C
      Short name=GAPDH-C
    EC=1.2.1.12

Gene names

Name:	gapC
Ordered Locus Names:	Z2304, ECs2022

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Homotetramer By similarity.
Subcellular location	CytoplasmPotential.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 333	333	Glyceraldehyde-3-phosphate dehydrogenase C		PRO_0000145652
Regions
	Nucleotide binding	11 – 12	2	NAD By similarity
	Region	149 – 151	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	209 – 210	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	150	1	Nucleophile By similarity
	Binding site	34	1	NAD By similarity
	Binding site	78	1	NAD; via carbonyl oxygen By similarity
	Binding site	180	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	232	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	315	1	NAD By similarity
	Site	177	1	Activates thiol group during catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P58072-1 [UniParc]. Last modified May 4, 2001. Version 1. Checksum: A2F77CB2E773E64C	FASTA	333	35,763
	10 20 30 40 50 60 MSKVGINGFG RIGRLVLRRL LEVKSNIDVV AINDLTSPKI LAYLLKHDSN YGPFPWSVDF 70 80 90 100 110 120 TEDSLIVDGK SIAVYAEKEA KNIPWKAKGA EIIVECTGFY TSAEKSQAHL DAGAKKVLIS 130 140 150 160 170 180 APAGEMKTIV YKVNDDTLDG NDTIVSVASC TTNCLAPMAK ALHDSFGIEV GTMTTIHAYT 190 200 210 220 230 240 GTQSLVDGPR GKDLRASRAA AENIIPHTTG AAKAIGLVIP ELSGKLKGHA QRVPVKTGSV 250 260 270 280 290 300 TELVSILGKK VTAEEVNNAL KQATTNNESF GYTDEEIVSS DIIGSHFGSV FDATQTEITA 310 320 330 VGDLQLVKTV AWYDNEYGFV TQLIRTLEKF AKL

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005174 Genomic DNA. Translation: AAG56359.1. BA000007 Genomic DNA. Translation: BAB35445.1.
PIR	C85737. F90881.
RefSeq	NP_287745.1. NP_310049.1.
3D structure databases
HSSP	HSSP built from PDB template 1HDG based on UniProtKB P17721.
ModBase	Search...
Genome annotation databases
GeneID	917219. 960993.
GenomeReviews	Gene locus ECs2022 in contig BA000007_GR. Gene locus Z2304 in contig AE005174_GR.
KEGG	ece:Z2304. ecs:ECs2022.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P58072.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS2022-MON.
Family and domain databases
InterPro	IPR000173. GlycerAld_3-P_DHase. IPR006424. Glyceraldehyde-3-P_DHase_1. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	P58072.

Entry information

Entry name

G3P3_ECO57

Accession

Primary (citable) accession number: P58072

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 4, 2001
Last sequence update:	May 4, 2001
Last modified:	November 25, 2008
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)