P58067 (NAGZ_ECO57) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-hexosaminidase
EC=3.2.1.52

Alternative name(s):
Beta-N-acetylhexosaminidase
N-acetyl-beta-glucosaminidase

Gene names

Name:	nagZ
Ordered Locus Names:	Z1746, ECs1485

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves GlcNAc linked beta-1,4 to MurNAc tripeptides By similarity. HAMAP MF_00364
Catalytic activity	Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides. HAMAP MF_00364
Pathway	Cell wall biogenesis; peptidoglycan recycling. HAMAP MF_00364
Subunit structure	Monomer Potential.
Subcellular location	Cytoplasm By similarity HAMAP MF_00364.
Sequence similarities	Belongs to the glycosyl hydrolase 3 family. NagZ subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-N-acetylhexosaminidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Beta-hexosaminidase HAMAP MF_00364		PRO_0000210787
Sites
	Active site	248	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P58067 [UniParc]. Last modified May 4, 2001. Version 1. Checksum: 71E28C6C39E8CAE0	FASTA	341	37,605
	10 20 30 40 50 60 MGPVMLDVEG YELDAEEREI LAHPLVGGLI LFTRNYHDPA QLRELVRQIR AASRNHLVVA 70 80 90 100 110 120 VDQEGGRVQR FREGFTRLPA AQSFAALLGM EEGGKLAQEA GWLMASEMIA MDIDISFAPV 130 140 150 160 170 180 LDVGHISAAI GERSYHADPE KALAIASRFI DGMHEAGMKT TGKHFPGHGA VTADSHKETP 190 200 210 220 230 240 CDPRPQAEIR AKDMSVFSTL IRENKLDAIM PAHVIYSDVD PRPASGSSYW LKTVLRQELG 250 260 270 280 290 300 FDGVIFSDDL SMEGAAIMGS YAERGQVSLD AGCDMILVCN NRKGAVSVLD NLSPIKAERV 310 320 330 340 TRLYHKGSFS RQELMDSARW KAISARLNQL HERWQEEKAG H

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG55853.1. BA000007 Genomic DNA. Translation: BAB34908.1.
PIR	A85674. E90814.
RefSeq	NP_287241.1. NC_002655.2. NP_309512.1. NC_002695.1.
3D structure databases
ProteinModelPortal	P58067.
SMR	P58067. Positions 1-325.
ModBase	Search...
Protein family/group databases
CAZy	GH3. Glycoside Hydrolase Family 3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000027083; EBESCP00000025976; EBESCG00000026135. EBESCT00000059942; EBESCP00000057770; EBESCG00000058989.
GeneID	913266. 959459.
GenomeReviews	Gene locus Z1746 in contig AE005174_GR. Gene locus ECs1485 in contig BA000007_GR.
KEGG	ece:Z1746. ecs:ECs1485.
PATRIC	18352211. VBIEscCol44059_1332.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000011903.
HOGENOM	HBG617255.
OMA	DLTMEGA.
ProtClustDB	PRK05337.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS1485-MONOMER.
Family and domain databases
HAMAP	MF_00364. NagZ. [Tree]
InterPro	IPR022956. Beta_hexosaminidase. IPR019800. Glyco_hydro_3_AS. IPR001764. Glyco_hydro_3_N. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.300. Glyco_hydro_3_N. 1 hit.
KO	K01207.
Pfam	PF00933. Glyco_hydro_3. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00775. GLYCOSYL_HYDROL_F3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAGZ_ECO57

Accession

Primary (citable) accession number: P58067

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 4, 2001
Last sequence update:	May 4, 2001
Last modified:	January 25, 2012
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program