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P58067

- NAGZ_ECO57

UniProt

P58067 - NAGZ_ECO57

Protein

Beta-hexosaminidase

Gene

nagZ

Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (04 May 2001)
      Previous versions | rss
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    Functioni

    Plays a role in peptidoglycan recycling by cleaving the terminal beta-1,4-linked N-acetylglucosamine (GlcNAc) from peptide-linked peptidoglycan fragments, giving rise to free GlcNAc, anhydro-N-acetylmuramic acid and anhydro-N-acetylmuramic acid-linked peptides.UniRule annotation

    Catalytic activityi

    Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei62 – 621SubstrateUniRule annotation
    Binding sitei70 – 701SubstrateUniRule annotation
    Binding sitei133 – 1331SubstrateUniRule annotation
    Sitei174 – 1741Important for catalytic activityUniRule annotation
    Active sitei176 – 1761Proton donor/acceptorUniRule annotation
    Active sitei248 – 2481NucleophileUniRule annotation

    GO - Molecular functioni

    1. beta-N-acetylhexosaminidase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. cell cycle Source: UniProtKB-KW
    3. cell division Source: UniProtKB-KW
    4. peptidoglycan biosynthetic process Source: UniProtKB-KW
    5. peptidoglycan turnover Source: UniProtKB-HAMAP
    6. regulation of cell shape Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

    Enzyme and pathway databases

    BioCyciECOL386585:GJFA-1471-MONOMER.
    UniPathwayiUPA00544.

    Protein family/group databases

    CAZyiGH3. Glycoside Hydrolase Family 3.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-hexosaminidaseUniRule annotation (EC:3.2.1.52UniRule annotation)
    Alternative name(s):
    Beta-N-acetylhexosaminidaseUniRule annotation
    N-acetyl-beta-glucosaminidaseUniRule annotation
    Gene namesi
    Name:nagZUniRule annotation
    Ordered Locus Names:Z1746, ECs1485
    OrganismiEscherichia coli O157:H7
    Taxonomic identifieri83334 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 341341Beta-hexosaminidasePRO_0000210787Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.Curated

    Protein-protein interaction databases

    STRINGi155864.Z1746.

    Structurei

    3D structure databases

    ProteinModelPortaliP58067.
    SMRiP58067. Positions 1-325.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni163 – 1642Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 3 family. NagZ subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1472.
    HOGENOMiHOG000248526.
    KOiK01207.
    OMAiAHDIDLS.
    OrthoDBiEOG6BCT06.

    Family and domain databases

    Gene3Di3.20.20.300. 1 hit.
    HAMAPiMF_00364. NagZ.
    InterProiIPR022956. Beta_hexosaminidase_bac.
    IPR019800. Glyco_hydro_3_AS.
    IPR001764. Glyco_hydro_3_N.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00933. Glyco_hydro_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00775. GLYCOSYL_HYDROL_F3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P58067-1 [UniParc]FASTAAdd to Basket

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    MGPVMLDVEG YELDAEEREI LAHPLVGGLI LFTRNYHDPA QLRELVRQIR    50
    AASRNHLVVA VDQEGGRVQR FREGFTRLPA AQSFAALLGM EEGGKLAQEA 100
    GWLMASEMIA MDIDISFAPV LDVGHISAAI GERSYHADPE KALAIASRFI 150
    DGMHEAGMKT TGKHFPGHGA VTADSHKETP CDPRPQAEIR AKDMSVFSTL 200
    IRENKLDAIM PAHVIYSDVD PRPASGSSYW LKTVLRQELG FDGVIFSDDL 250
    SMEGAAIMGS YAERGQVSLD AGCDMILVCN NRKGAVSVLD NLSPIKAERV 300
    TRLYHKGSFS RQELMDSARW KAISARLNQL HERWQEEKAG H 341
    Length:341
    Mass (Da):37,605
    Last modified:May 4, 2001 - v1
    Checksum:i71E28C6C39E8CAE0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005174 Genomic DNA. Translation: AAG55853.1.
    BA000007 Genomic DNA. Translation: BAB34908.1.
    PIRiA85674.
    E90814.
    RefSeqiNP_287241.1. NC_002655.2.
    NP_309512.1. NC_002695.1.

    Genome annotation databases

    EnsemblBacteriaiAAG55853; AAG55853; Z1746.
    BAB34908; BAB34908; BAB34908.
    GeneIDi913266.
    959459.
    KEGGiece:Z1746.
    ecs:ECs1485.
    PATRICi18352211. VBIEscCol44059_1332.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005174 Genomic DNA. Translation: AAG55853.1 .
    BA000007 Genomic DNA. Translation: BAB34908.1 .
    PIRi A85674.
    E90814.
    RefSeqi NP_287241.1. NC_002655.2.
    NP_309512.1. NC_002695.1.

    3D structure databases

    ProteinModelPortali P58067.
    SMRi P58067. Positions 1-325.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 155864.Z1746.

    Protein family/group databases

    CAZyi GH3. Glycoside Hydrolase Family 3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG55853 ; AAG55853 ; Z1746 .
    BAB34908 ; BAB34908 ; BAB34908 .
    GeneIDi 913266.
    959459.
    KEGGi ece:Z1746.
    ecs:ECs1485.
    PATRICi 18352211. VBIEscCol44059_1332.

    Phylogenomic databases

    eggNOGi COG1472.
    HOGENOMi HOG000248526.
    KOi K01207.
    OMAi AHDIDLS.
    OrthoDBi EOG6BCT06.

    Enzyme and pathway databases

    UniPathwayi UPA00544 .
    BioCyci ECOL386585:GJFA-1471-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.300. 1 hit.
    HAMAPi MF_00364. NagZ.
    InterProi IPR022956. Beta_hexosaminidase_bac.
    IPR019800. Glyco_hydro_3_AS.
    IPR001764. Glyco_hydro_3_N.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF00933. Glyco_hydro_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00775. GLYCOSYL_HYDROL_F3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
    2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
      Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
      , Kuhara S., Shiba T., Hattori M., Shinagawa H.
      DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

    Entry informationi

    Entry nameiNAGZ_ECO57
    AccessioniPrimary (citable) accession number: P58067
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 4, 2001
    Last sequence update: May 4, 2001
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3