P58057 (PPNK_ECO57) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable inorganic polyphosphate/ATP-NAD kinase
Short name=Poly(P)/ATP NAD kinase
EC=2.7.1.23

Gene names

Name:	ppnK
Ordered Locus Names:	Z3908, ECs3477

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus By similarity. HAMAP-Rule MF_00361
Catalytic activity	ATP + NAD+ = ADP + NADP+. HAMAP-Rule MF_00361
Cofactor	Divalent metal ions By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the NAD kinase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding NAD NADP Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	NAD metabolic process Inferred from electronic annotation. Source: InterPro NADP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ kinase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	Probable inorganic polyphosphate/ATP-NAD kinase HAMAP-Rule MF_00361		PRO_0000120618

Sequences

	Sequence		Length	Mass (Da)
	P58057 [UniParc]. Last modified May 4, 2001. Version 1. Checksum: 30BE8E44DA060915	FASTA	292	32,596
	10 20 30 40 50 60 MNNHFKCIGI VGHPRHPTAL TTHEMLYRWL CTKGYEVIVE QQIAHELQLK NVKTGTLAEI 70 80 90 100 110 120 GQLADLAVVV GGDGNMLGAT RTLARYDIKV IGINRGNLGF LTDLDPDNAQ QQLADVLEGH 130 140 150 160 170 180 YISEKRFLLE AQVCQQDCQK RISTAINEVV LHPGKVAHMI EFEVYIDEIF AFSQRSDGLI 190 200 210 220 230 240 ISTPTGSTAY SLSAGGPILT PSLDAITLVP MFPHTLSARP LVINSSSTIR LRFSHRRNDL 250 260 270 280 290 EISCDSQIAL PIQEGEDVLI RRCDYHLNLI HPKDYSYFNT LSTKLGWSKK LF

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG57725.1. BA000007 Genomic DNA. Translation: BAB36900.1.
PIR	A85908. E91063.
RefSeq	NP_289167.1. NC_002655.2. NP_311504.1. NC_002695.1.
3D structure databases
ProteinModelPortal	P58057.
SMR	P58057. Positions 4-292.
ModBase	Search...
Protein-protein interaction databases
STRING	155864.Z3908.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAG57725; AAG57725; Z3908. BAB36900; BAB36900; BAB36900.
GeneID	914811. 958034.
KEGG	ece:Z3908. ecs:ECs3477.
PATRIC	18356400. VBIEscCol44059_3390.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0061.
HOGENOM	HOG000227221.
KO	K00858.
OMA	LLWGESP.
ProtClustDB	PRK03378.
Enzyme and pathway databases
BioCyc	ECOL386585:GJFA-3443-MONOMER.
Family and domain databases
Gene3D	2.60.200.30. 1 hit. 3.40.50.10330. 1 hit.
HAMAP	MF_00361. NAD_kinase.
InterPro	IPR017438. ATP-NAD_kinase_dom_1. IPR016064. ATP-NAD_kinase_PpnK-typ. IPR017437. ATP-NAD_kinase_PpnK-typ_all-b. IPR002504. PolyP/ATP_NADK_prd. [Graphical view]
PANTHER	PTHR20275. PTHR20275. 1 hit.
Pfam	PF01513. NAD_kinase. 1 hit. [Graphical view]
SUPFAM	SSF111331. ATP-NAD_kinase_PpnK-typ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PPNK_ECO57

Accession

Primary (citable) accession number: P58057

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 4, 2001
Last sequence update:	May 4, 2001
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program