Reviewed, UniProtKB/Swiss-Prot P58054 (YBEM_ECO57)

Names and origin

Protein names

Recommended name:
    UPF0012 hydrolase ybeM
    EC=3.5.-.-

Gene names

Name:	ybeM
Ordered Locus Names:	Z0771, ECs0664

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	262 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Sequence similarities

Belongs to the UPF0012 family. Contains 1 CN hydrolase domain.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nitrogen compound metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 262	262	UPF0012 hydrolase ybeM		PRO_0000213259
Regions
	Domain	1 – 260	260	CN hydrolase
Sites
	Active site	40	1	Proton acceptor Potential
	Active site	110	1	By similarity
	Active site	147	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	P58054-1 [UniParc]. Last modified May 4, 2001. Version 1. Checksum: 74CF04FD3B9CC1DF	FASTA	262	28,681
	10 20 30 40 50 60 MLVAAGQFAV TSVWEKNAEI CASLMAQAAE NDVSLFVLPE ALLARDDHDA DLSVKSAQLL 70 80 90 100 110 120 EGEFLGRLRR ESKRNMMTTI LTIHVPSTPG RAWNMLVALQ AGNIVARYAK LHLYDAFAIQ 130 140 150 160 170 180 ESRRVDAGNE IAPLLEVEGM KVGLMTCYDL RFPELALAQA LQGAEILVLP AAWVRGPLKE 190 200 210 220 230 240 HHWSTLLAAR ALDTTCYMVA AGECGNKNIG QSRIIDPFGV TIAAASEMPA LIMAEVTPER 250 260 VRQVRAQLPV LNNRRFAPPQ LL

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005174 Genomic DNA. Translation: AAG54960.1. Different initiation. BA000007 Genomic DNA. Translation: BAB34087.1.
PIR	D85562. H90711.
RefSeq	NP_286352.1. NP_308691.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	917024. 957658.
GenomeReviews	Gene locus Z0771 in contig AE005174_GR. Gene locus ECs0664 in contig BA000007_GR.
KEGG	ece:Z0771. ecs:ECs0664.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG742418.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS0664-MON.
Family and domain databases
InterPro	IPR003010. Ntlse/CNhydtse. IPR001110. UPF0012_CS. [Graphical view]
Gene3D	G3DSA:3.60.110.10. Ntlse/CNhydtse. 1 hit.
Pfam	PF00795. CN_hydrolase. 1 hit. [Graphical view]
PROSITE	PS50263. CN_HYDROLASE. 1 hit. PS01227. UPF0012. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YBEM_ECO57

Accession

Primary (citable) accession number: P58054

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 4, 2001
Last sequence update:	May 4, 2001
Last modified:	December 15, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)