P58032 (FLPA_SULSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase
Short name=FIB
EC=2.1.1.-

Gene names

Name:	flpA
Ordered Locus Names:	SSO0940
ORF Names:	C33_014

Organism

Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) [Reference proteome] [HAMAP]

Taxonomic identifier

273057 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus › Sulfolobus solfataricus

Protein attributes

Sequence length	232 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in pre-rRNA and tRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in rRNA and tRNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Ref.2 Ref.3
Subunit structure	Interacts with nop5. Component of box C/D small ribonucleoprotein (sRNP) particles that contain rpl7ae, FlpA and nop5, plus a guide RNA. Ref.2
Sequence similarities	Belongs to the methyltransferase superfamily. Fibrillarin family.

Ontologies

Keywords
Biological process	rRNA processing tRNA processing
Ligand	RNA-binding
Molecular function	Methyltransferase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	rRNA processing Inferred from electronic annotation. Source: HAMAP tRNA processing Inferred from electronic annotation. Source: HAMAP
Molecular_function	RNA binding Inferred from electronic annotation. Source: HAMAP methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SSO0939	Q97ZH3	2	EBI-2944159,EBI-2944135

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 232

232

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase HAMAP-Rule MF_00351

PRO_0000148549

Regions

Region

89 – 90

2

S-adenosyl-L-methionine binding By similarity

Region

108 – 109

2

S-adenosyl-L-methionine binding By similarity

Region

133 – 134

2

S-adenosyl-L-methionine binding By similarity

Sites

Binding site

60

1

S-adenosyl-L-methionine By similarity

Binding site

153

1

S-adenosyl-L-methionine By similarity

Experimental info

Mutagenesis

85

1

A → V: Loss of methyltransferase activity. Ref.2

Mutagenesis

129

1

P → A: Decreased methyltransferase activity. Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

232

Helix Strand Turn

Details...

Beta strand

6 – 9

4

Beta strand

15 – 19

5

Beta strand

21 – 23

3

Beta strand

25 – 30

6

Beta strand

38 – 40

3

Beta strand

43 – 46

4

Beta strand

49 – 53

5

Turn

56 – 58

3

Helix

60 – 66

7

Beta strand

79 – 83

5

Helix

89 – 98

10

Turn

99 – 101

3

Beta strand

102 – 108

7

Helix

111 – 123

13

Beta strand

127 – 131

5

Helix

137 – 139

3

Turn

140 – 143

4

Beta strand

147 – 152

6

Helix

159 – 170

12

Beta strand

171 – 181

11

Helix

183 – 186

4

Beta strand

188 – 190

3

Beta strand

192 – 195

4

Helix

198 – 205

8

Beta strand

208 – 215

8

Turn

217 – 219

3

Beta strand

224 – 230

7

Sequences

	Sequence		Length	Mass (Da)
	P58032 [UniParc]. Last modified April 27, 2001. Version 1. Checksum: CFFB34D2D4FA9CF2	FASTA	232	26,422
	10 20 30 40 50 60 MSEVITVKQT NMENIYECEF NDGSFRLCTR NLVPNFNVYG ERLIKYEGVE YREWNAFRSK 70 80 90 100 110 120 LAGAILKGLK TNPIRKGTKV LYLGAASGTT ISHVSDIIEL NGKAYGVEFS PRVVRELLLV 130 140 150 160 170 180 AQRRPNIFPL LADARFPQSY KSVVENVDVL YVDIAQPDQT DIAIYNAKFF LKVNGDMLLV 190 200 210 220 230 IKARSIDVTK DPKEIYKTEV EKLENSNFET IQIINLDPYD KDHAIVLSKY KG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome of the crenarchaeon Sulfolobus solfataricus P2." She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J. Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
[2]	"In vitro reconstitution and activity of a C/D box methylation guide ribonucleoprotein complex." Omer A.D., Ziesche S., Ebhardt H., Dennis P.P. Proc. Natl. Acad. Sci. U.S.A. 99:5289-5294(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF ALA-85 AND PRO-129, SUBUNIT.
[3]	"RNA-guided nucleotide modification of ribosomal and non-ribosomal RNAs in Archaea." Ziesche S.M., Omer A.D., Dennis P.P. Mol. Microbiol. 54:980-993(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE006641 Genomic DNA. Translation: AAK41216.1.

PIR

A99245.

RefSeq

NP_342426.1. NC_002754.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3ID5	X-ray	4.01	B/F	3-232	[»]
3ID6	X-ray	2.60	C	3-232	[»]
3PLA	X-ray	3.15	E/F/M	3-232	[»]

ProteinModelPortal

P58032.

SMR

P58032. Positions 4-229.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-48939N.

IntAct

P58032. 2 interactions.

STRING

273057.SSO0940.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAK41216; AAK41216; SSO0940.

GeneID

1455183.

KEGG

sso:SSO0940.

Phylogenomic databases

eggNOG

COG1889.

HOGENOM

HOG000106741.

KO

K04795.

OMA

EFAPRIM.

ProtClustDB

PRK04266.

Family and domain databases

HAMAP

MF_00351. RNA_methyltransf_FlpA.

InterPro

IPR000692. Fibrillarin.
IPR020813. Fibrillarin_CS.
[Graphical view]

PANTHER

PTHR10335. PTHR10335. 1 hit.

Pfam

PF01269. Fibrillarin. 1 hit.
[Graphical view]

PIRSF

PIRSF006540. Nop17p. 1 hit.

PRINTS

PR00052. FIBRILLARIN.

PROSITE

PS00566. FIBRILLARIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P58032.

Entry information

Entry name

FLPA_SULSO

Accession

Primary (citable) accession number: P58032

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	April 27, 2001
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program