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P58032

- FLPA_SULSO

UniProt

P58032 - FLPA_SULSO

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Protein
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase
Gene
flpA, SSO0940, C33_014
Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in pre-rRNA and tRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in rRNA and tRNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA.3 Publications

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-HAMAP
  2. methyltransferase activity Source: UniProtKB-HAMAP
  3. protein binding Source: IntAct

GO - Biological processi

  1. rRNA processing Source: UniProtKB-HAMAP
  2. tRNA processing Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing, tRNA processing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciSSOL273057:GCH2-893-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase (EC:2.1.1.-)
Short name:
FIB
Gene namesi
Name:flpA
Ordered Locus Names:SSO0940
ORF Names:C33_014
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
ProteomesiUP000001974: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi85 – 851A → V: Loss of methyltransferase activity. 1 Publication
Mutagenesisi129 – 1291P → A: Decreased methyltransferase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 232232Fibrillarin-like rRNA/tRNA 2'-O-methyltransferaseUniRule annotation
PRO_0000148549Add
BLAST

Interactioni

Subunit structurei

Interacts with nop5. Component of box C/D small ribonucleoprotein (sRNP) particles that contain rpl7ae, FlpA and nop5, plus a guide RNA. These sRNP particles form homodimers, giving rise to an asymmetric holoenzyme.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SSO0939Q97ZH32EBI-2944159,EBI-2944135

Protein-protein interaction databases

DIPiDIP-48939N.
IntActiP58032. 2 interactions.
STRINGi273057.SSO0940.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 94
Beta strandi15 – 195
Beta strandi21 – 233
Beta strandi25 – 306
Beta strandi38 – 403
Beta strandi43 – 464
Beta strandi49 – 535
Turni56 – 583
Helixi60 – 667
Beta strandi79 – 835
Helixi89 – 9810
Turni99 – 1013
Beta strandi102 – 1087
Helixi111 – 12313
Beta strandi127 – 1315
Helixi137 – 1393
Turni140 – 1434
Beta strandi147 – 1526
Helixi159 – 17012
Beta strandi171 – 18111
Helixi183 – 1864
Beta strandi188 – 1903
Beta strandi192 – 1954
Helixi198 – 2058
Beta strandi208 – 2158
Turni217 – 2193
Beta strandi224 – 2307

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ID5X-ray4.01B/F1-232[»]
3ID6X-ray2.60C1-232[»]
3PLAX-ray3.15E/F/M1-232[»]
ProteinModelPortaliP58032.
SMRiP58032. Positions 4-229.

Miscellaneous databases

EvolutionaryTraceiP58032.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni89 – 902S-adenosyl-L-methionine bindingUniRule annotation
Regioni108 – 1092S-adenosyl-L-methionine bindingUniRule annotation
Regioni133 – 1342S-adenosyl-L-methionine bindingUniRule annotation
Regioni153 – 1564S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1889.
HOGENOMiHOG000106741.
KOiK04795.
OMAiWNPNKSK.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00351. RNA_methyltransf_FlpA.
InterProiIPR000692. Fibrillarin.
IPR020813. Fibrillarin_CS.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF01269. Fibrillarin. 1 hit.
[Graphical view]
PIRSFiPIRSF006540. Nop17p. 1 hit.
PRINTSiPR00052. FIBRILLARIN.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00566. FIBRILLARIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P58032-1 [UniParc]FASTAAdd to Basket

« Hide

MSEVITVKQT NMENIYECEF NDGSFRLCTR NLVPNFNVYG ERLIKYEGVE    50
YREWNAFRSK LAGAILKGLK TNPIRKGTKV LYLGAASGTT ISHVSDIIEL 100
NGKAYGVEFS PRVVRELLLV AQRRPNIFPL LADARFPQSY KSVVENVDVL 150
YVDIAQPDQT DIAIYNAKFF LKVNGDMLLV IKARSIDVTK DPKEIYKTEV 200
EKLENSNFET IQIINLDPYD KDHAIVLSKY KG 232
Length:232
Mass (Da):26,422
Last modified:April 27, 2001 - v1
Checksum:iCFFB34D2D4FA9CF2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006641 Genomic DNA. Translation: AAK41216.1.
PIRiA99245.
RefSeqiNP_342426.1. NC_002754.1.
WP_009992372.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK41216; AAK41216; SSO0940.
GeneIDi1455183.
KEGGisso:SSO0940.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006641 Genomic DNA. Translation: AAK41216.1 .
PIRi A99245.
RefSeqi NP_342426.1. NC_002754.1.
WP_009992372.1. NC_002754.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3ID5 X-ray 4.01 B/F 1-232 [» ]
3ID6 X-ray 2.60 C 1-232 [» ]
3PLA X-ray 3.15 E/F/M 1-232 [» ]
ProteinModelPortali P58032.
SMRi P58032. Positions 4-229.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-48939N.
IntActi P58032. 2 interactions.
STRINGi 273057.SSO0940.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK41216 ; AAK41216 ; SSO0940 .
GeneIDi 1455183.
KEGGi sso:SSO0940.

Phylogenomic databases

eggNOGi COG1889.
HOGENOMi HOG000106741.
KOi K04795.
OMAi WNPNKSK.

Enzyme and pathway databases

BioCyci SSOL273057:GCH2-893-MONOMER.

Miscellaneous databases

EvolutionaryTracei P58032.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_00351. RNA_methyltransf_FlpA.
InterProi IPR000692. Fibrillarin.
IPR020813. Fibrillarin_CS.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF01269. Fibrillarin. 1 hit.
[Graphical view ]
PIRSFi PIRSF006540. Nop17p. 1 hit.
PRINTSi PR00052. FIBRILLARIN.
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS00566. FIBRILLARIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
  2. "In vitro reconstitution and activity of a C/D box methylation guide ribonucleoprotein complex."
    Omer A.D., Ziesche S., Ebhardt H., Dennis P.P.
    Proc. Natl. Acad. Sci. U.S.A. 99:5289-5294(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF ALA-85 AND PRO-129, SUBUNIT.
  3. "RNA-guided nucleotide modification of ribosomal and non-ribosomal RNAs in Archaea."
    Ziesche S.M., Omer A.D., Dennis P.P.
    Mol. Microbiol. 54:980-993(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Structural organization of box C/D RNA-guided RNA methyltransferase."
    Ye K., Jia R., Lin J., Ju M., Peng J., Xu A., Zhang L.
    Proc. Natl. Acad. Sci. U.S.A. 106:13808-13813(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) IN COMPLEXES WITH RNA; S-ADENOSYL-L-METHIONINE; NOP5 AND RPL7AE, SUBUNIT.
  5. "Structural basis for site-specific ribose methylation by box C/D RNA protein complexes."
    Lin J., Lai S., Jia R., Xu A., Zhang L., Lu J., Ye K.
    Nature 469:559-563(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.15 ANGSTROMS) IN COMPLEX WITH GUIDE RNA; SUBSTRATE RNA AND RPL7AE, SUBUNIT, FUNCTION.

Entry informationi

Entry nameiFLPA_SULSO
AccessioniPrimary (citable) accession number: P58032
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: April 27, 2001
Last modified: September 3, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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