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P58032

- FLPA_SULSO

UniProt

P58032 - FLPA_SULSO

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Protein

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase

Gene

flpA

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Involved in pre-rRNA and tRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in rRNA and tRNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA.3 PublicationsUniRule annotation

GO - Molecular functioni

  1. methyltransferase activity Source: UniProtKB-HAMAP
  2. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. rRNA processing Source: UniProtKB-HAMAP
  2. tRNA processing Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing, tRNA processing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciSSOL273057:GCH2-893-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferaseUniRule annotation (EC:2.1.1.-UniRule annotation)
Short name:
FIB
Gene namesi
Name:flpAUniRule annotation
Ordered Locus Names:SSO0940
ORF Names:C33_014
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
ProteomesiUP000001974: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi85 – 851A → V: Loss of methyltransferase activity. 1 Publication
Mutagenesisi129 – 1291P → A: Decreased methyltransferase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 232232Fibrillarin-like rRNA/tRNA 2'-O-methyltransferasePRO_0000148549Add
BLAST

Interactioni

Subunit structurei

Interacts with nop5. Component of box C/D small ribonucleoprotein (sRNP) particles that contain rpl7ae, FlpA and nop5, plus a guide RNA. These sRNP particles form homodimers, giving rise to an asymmetric holoenzyme.3 PublicationsUniRule annotation

Binary interactionsi

WithEntry#Exp.IntActNotes
SSO0939Q97ZH32EBI-2944159,EBI-2944135

Protein-protein interaction databases

DIPiDIP-48939N.
IntActiP58032. 2 interactions.
STRINGi273057.SSO0940.

Structurei

Secondary structure

1
232
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 94Combined sources
Beta strandi15 – 195Combined sources
Beta strandi21 – 233Combined sources
Beta strandi25 – 306Combined sources
Beta strandi38 – 403Combined sources
Beta strandi43 – 464Combined sources
Beta strandi49 – 535Combined sources
Turni56 – 583Combined sources
Helixi60 – 667Combined sources
Beta strandi79 – 835Combined sources
Helixi89 – 9810Combined sources
Turni99 – 1013Combined sources
Beta strandi102 – 1087Combined sources
Helixi111 – 12313Combined sources
Beta strandi127 – 1315Combined sources
Helixi137 – 1393Combined sources
Turni140 – 1434Combined sources
Beta strandi147 – 1526Combined sources
Helixi159 – 17012Combined sources
Beta strandi171 – 18111Combined sources
Helixi183 – 1864Combined sources
Beta strandi188 – 1903Combined sources
Beta strandi192 – 1954Combined sources
Helixi198 – 2058Combined sources
Beta strandi208 – 2158Combined sources
Turni217 – 2193Combined sources
Beta strandi224 – 2307Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ID5X-ray4.01B/F1-232[»]
3ID6X-ray2.60C1-232[»]
3PLAX-ray3.15E/F/M1-232[»]
ProteinModelPortaliP58032.
SMRiP58032. Positions 4-229.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP58032.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni89 – 902S-adenosyl-L-methionine binding
Regioni108 – 1092S-adenosyl-L-methionine binding
Regioni133 – 1342S-adenosyl-L-methionine binding
Regioni153 – 1564S-adenosyl-L-methionine binding

Sequence similaritiesi

Belongs to the methyltransferase superfamily. Fibrillarin family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1889.
HOGENOMiHOG000106741.
InParanoidiP58032.
KOiK04795.
OMAiWNPNKSK.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00351. RNA_methyltransf_FlpA.
InterProiIPR000692. Fibrillarin.
IPR020813. Fibrillarin_CS.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF01269. Fibrillarin. 1 hit.
[Graphical view]
PIRSFiPIRSF006540. Nop17p. 1 hit.
PRINTSiPR00052. FIBRILLARIN.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00566. FIBRILLARIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P58032-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSEVITVKQT NMENIYECEF NDGSFRLCTR NLVPNFNVYG ERLIKYEGVE
60 70 80 90 100
YREWNAFRSK LAGAILKGLK TNPIRKGTKV LYLGAASGTT ISHVSDIIEL
110 120 130 140 150
NGKAYGVEFS PRVVRELLLV AQRRPNIFPL LADARFPQSY KSVVENVDVL
160 170 180 190 200
YVDIAQPDQT DIAIYNAKFF LKVNGDMLLV IKARSIDVTK DPKEIYKTEV
210 220 230
EKLENSNFET IQIINLDPYD KDHAIVLSKY KG
Length:232
Mass (Da):26,422
Last modified:April 27, 2001 - v1
Checksum:iCFFB34D2D4FA9CF2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK41216.1.
PIRiA99245.
RefSeqiNP_342426.1. NC_002754.1.
WP_009992372.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK41216; AAK41216; SSO0940.
GeneIDi1455183.
KEGGisso:SSO0940.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK41216.1 .
PIRi A99245.
RefSeqi NP_342426.1. NC_002754.1.
WP_009992372.1. NC_002754.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3ID5 X-ray 4.01 B/F 1-232 [» ]
3ID6 X-ray 2.60 C 1-232 [» ]
3PLA X-ray 3.15 E/F/M 1-232 [» ]
ProteinModelPortali P58032.
SMRi P58032. Positions 4-229.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-48939N.
IntActi P58032. 2 interactions.
STRINGi 273057.SSO0940.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK41216 ; AAK41216 ; SSO0940 .
GeneIDi 1455183.
KEGGi sso:SSO0940.

Phylogenomic databases

eggNOGi COG1889.
HOGENOMi HOG000106741.
InParanoidi P58032.
KOi K04795.
OMAi WNPNKSK.

Enzyme and pathway databases

BioCyci SSOL273057:GCH2-893-MONOMER.

Miscellaneous databases

EvolutionaryTracei P58032.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_00351. RNA_methyltransf_FlpA.
InterProi IPR000692. Fibrillarin.
IPR020813. Fibrillarin_CS.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF01269. Fibrillarin. 1 hit.
[Graphical view ]
PIRSFi PIRSF006540. Nop17p. 1 hit.
PRINTSi PR00052. FIBRILLARIN.
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS00566. FIBRILLARIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
  2. "In vitro reconstitution and activity of a C/D box methylation guide ribonucleoprotein complex."
    Omer A.D., Ziesche S., Ebhardt H., Dennis P.P.
    Proc. Natl. Acad. Sci. U.S.A. 99:5289-5294(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF ALA-85 AND PRO-129, SUBUNIT.
  3. "RNA-guided nucleotide modification of ribosomal and non-ribosomal RNAs in Archaea."
    Ziesche S.M., Omer A.D., Dennis P.P.
    Mol. Microbiol. 54:980-993(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Structural organization of box C/D RNA-guided RNA methyltransferase."
    Ye K., Jia R., Lin J., Ju M., Peng J., Xu A., Zhang L.
    Proc. Natl. Acad. Sci. U.S.A. 106:13808-13813(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) IN COMPLEXES WITH RNA; S-ADENOSYL-L-METHIONINE; NOP5 AND RPL7AE, SUBUNIT.
  5. "Structural basis for site-specific ribose methylation by box C/D RNA protein complexes."
    Lin J., Lai S., Jia R., Xu A., Zhang L., Lu J., Ye K.
    Nature 469:559-563(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.15 ANGSTROMS) IN COMPLEX WITH GUIDE RNA; SUBSTRATE RNA AND RPL7AE, SUBUNIT, FUNCTION.

Entry informationi

Entry nameiFLPA_SULSO
AccessioniPrimary (citable) accession number: P58032
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: April 27, 2001
Last modified: November 26, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3