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P58032

- FLPA_SULSO

UniProt

P58032 - FLPA_SULSO

Protein

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase

Gene

flpA

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (27 Apr 2001)
      Previous versions | rss
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    Functioni

    Involved in pre-rRNA and tRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in rRNA and tRNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA.3 PublicationsUniRule annotation

    GO - Molecular functioni

    1. methyltransferase activity Source: UniProtKB-HAMAP
    2. protein binding Source: IntAct
    3. RNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. rRNA processing Source: UniProtKB-HAMAP
    2. tRNA processing Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    rRNA processing, tRNA processing

    Keywords - Ligandi

    RNA-binding

    Enzyme and pathway databases

    BioCyciSSOL273057:GCH2-893-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fibrillarin-like rRNA/tRNA 2'-O-methyltransferaseUniRule annotation (EC:2.1.1.-UniRule annotation)
    Short name:
    FIB
    Gene namesi
    Name:flpAUniRule annotation
    Ordered Locus Names:SSO0940
    ORF Names:C33_014
    OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
    Taxonomic identifieri273057 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
    ProteomesiUP000001974: Chromosome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi85 – 851A → V: Loss of methyltransferase activity. 1 Publication
    Mutagenesisi129 – 1291P → A: Decreased methyltransferase activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 232232Fibrillarin-like rRNA/tRNA 2'-O-methyltransferasePRO_0000148549Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with nop5. Component of box C/D small ribonucleoprotein (sRNP) particles that contain rpl7ae, FlpA and nop5, plus a guide RNA. These sRNP particles form homodimers, giving rise to an asymmetric holoenzyme.3 PublicationsUniRule annotation

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SSO0939Q97ZH32EBI-2944159,EBI-2944135

    Protein-protein interaction databases

    DIPiDIP-48939N.
    IntActiP58032. 2 interactions.
    STRINGi273057.SSO0940.

    Structurei

    Secondary structure

    1
    232
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi6 – 94
    Beta strandi15 – 195
    Beta strandi21 – 233
    Beta strandi25 – 306
    Beta strandi38 – 403
    Beta strandi43 – 464
    Beta strandi49 – 535
    Turni56 – 583
    Helixi60 – 667
    Beta strandi79 – 835
    Helixi89 – 9810
    Turni99 – 1013
    Beta strandi102 – 1087
    Helixi111 – 12313
    Beta strandi127 – 1315
    Helixi137 – 1393
    Turni140 – 1434
    Beta strandi147 – 1526
    Helixi159 – 17012
    Beta strandi171 – 18111
    Helixi183 – 1864
    Beta strandi188 – 1903
    Beta strandi192 – 1954
    Helixi198 – 2058
    Beta strandi208 – 2158
    Turni217 – 2193
    Beta strandi224 – 2307

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3ID5X-ray4.01B/F1-232[»]
    3ID6X-ray2.60C1-232[»]
    3PLAX-ray3.15E/F/M1-232[»]
    ProteinModelPortaliP58032.
    SMRiP58032. Positions 4-229.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP58032.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni89 – 902S-adenosyl-L-methionine binding
    Regioni108 – 1092S-adenosyl-L-methionine binding
    Regioni133 – 1342S-adenosyl-L-methionine binding
    Regioni153 – 1564S-adenosyl-L-methionine binding

    Sequence similaritiesi

    Belongs to the methyltransferase superfamily. Fibrillarin family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1889.
    HOGENOMiHOG000106741.
    KOiK04795.
    OMAiWNPNKSK.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_00351. RNA_methyltransf_FlpA.
    InterProiIPR000692. Fibrillarin.
    IPR020813. Fibrillarin_CS.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF01269. Fibrillarin. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006540. Nop17p. 1 hit.
    PRINTSiPR00052. FIBRILLARIN.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS00566. FIBRILLARIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P58032-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEVITVKQT NMENIYECEF NDGSFRLCTR NLVPNFNVYG ERLIKYEGVE    50
    YREWNAFRSK LAGAILKGLK TNPIRKGTKV LYLGAASGTT ISHVSDIIEL 100
    NGKAYGVEFS PRVVRELLLV AQRRPNIFPL LADARFPQSY KSVVENVDVL 150
    YVDIAQPDQT DIAIYNAKFF LKVNGDMLLV IKARSIDVTK DPKEIYKTEV 200
    EKLENSNFET IQIINLDPYD KDHAIVLSKY KG 232
    Length:232
    Mass (Da):26,422
    Last modified:April 27, 2001 - v1
    Checksum:iCFFB34D2D4FA9CF2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006641 Genomic DNA. Translation: AAK41216.1.
    PIRiA99245.
    RefSeqiNP_342426.1. NC_002754.1.
    WP_009992372.1. NC_002754.1.

    Genome annotation databases

    EnsemblBacteriaiAAK41216; AAK41216; SSO0940.
    GeneIDi1455183.
    KEGGisso:SSO0940.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006641 Genomic DNA. Translation: AAK41216.1 .
    PIRi A99245.
    RefSeqi NP_342426.1. NC_002754.1.
    WP_009992372.1. NC_002754.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3ID5 X-ray 4.01 B/F 1-232 [» ]
    3ID6 X-ray 2.60 C 1-232 [» ]
    3PLA X-ray 3.15 E/F/M 1-232 [» ]
    ProteinModelPortali P58032.
    SMRi P58032. Positions 4-229.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-48939N.
    IntActi P58032. 2 interactions.
    STRINGi 273057.SSO0940.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK41216 ; AAK41216 ; SSO0940 .
    GeneIDi 1455183.
    KEGGi sso:SSO0940.

    Phylogenomic databases

    eggNOGi COG1889.
    HOGENOMi HOG000106741.
    KOi K04795.
    OMAi WNPNKSK.

    Enzyme and pathway databases

    BioCyci SSOL273057:GCH2-893-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P58032.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_00351. RNA_methyltransf_FlpA.
    InterProi IPR000692. Fibrillarin.
    IPR020813. Fibrillarin_CS.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF01269. Fibrillarin. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006540. Nop17p. 1 hit.
    PRINTSi PR00052. FIBRILLARIN.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS00566. FIBRILLARIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
    2. "In vitro reconstitution and activity of a C/D box methylation guide ribonucleoprotein complex."
      Omer A.D., Ziesche S., Ebhardt H., Dennis P.P.
      Proc. Natl. Acad. Sci. U.S.A. 99:5289-5294(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF ALA-85 AND PRO-129, SUBUNIT.
    3. "RNA-guided nucleotide modification of ribosomal and non-ribosomal RNAs in Archaea."
      Ziesche S.M., Omer A.D., Dennis P.P.
      Mol. Microbiol. 54:980-993(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "Structural organization of box C/D RNA-guided RNA methyltransferase."
      Ye K., Jia R., Lin J., Ju M., Peng J., Xu A., Zhang L.
      Proc. Natl. Acad. Sci. U.S.A. 106:13808-13813(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) IN COMPLEXES WITH RNA; S-ADENOSYL-L-METHIONINE; NOP5 AND RPL7AE, SUBUNIT.
    5. "Structural basis for site-specific ribose methylation by box C/D RNA protein complexes."
      Lin J., Lai S., Jia R., Xu A., Zhang L., Lu J., Ye K.
      Nature 469:559-563(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.15 ANGSTROMS) IN COMPLEX WITH GUIDE RNA; SUBSTRATE RNA AND RPL7AE, SUBUNIT, FUNCTION.

    Entry informationi

    Entry nameiFLPA_SULSO
    AccessioniPrimary (citable) accession number: P58032
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 27, 2001
    Last sequence update: April 27, 2001
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3