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Protein

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase

Gene

flpA

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in pre-rRNA and tRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in rRNA and tRNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA.UniRule annotation3 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing, tRNA processing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferaseUniRule annotation (EC:2.1.1.-UniRule annotation)
Short name:
FIB
Gene namesi
Name:flpAUniRule annotation
Ordered Locus Names:SSO0940
ORF Names:C33_014
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001974 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi85A → V: Loss of methyltransferase activity. 1 Publication1
Mutagenesisi129P → A: Decreased methyltransferase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001485491 – 232Fibrillarin-like rRNA/tRNA 2'-O-methyltransferaseAdd BLAST232

Interactioni

Subunit structurei

Interacts with nop5. Component of box C/D small ribonucleoprotein (sRNP) particles that contain rpl7ae, FlpA and nop5, plus a guide RNA. These sRNP particles form homodimers, giving rise to an asymmetric holoenzyme.UniRule annotation3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SSO0939Q97ZH32EBI-2944159,EBI-2944135

Protein-protein interaction databases

DIPiDIP-48939N.
IntActiP58032. 2 interactors.
STRINGi273057.SSO0940.

Structurei

Secondary structure

1232
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 9Combined sources4
Beta strandi15 – 19Combined sources5
Beta strandi21 – 23Combined sources3
Beta strandi25 – 30Combined sources6
Beta strandi38 – 40Combined sources3
Beta strandi43 – 46Combined sources4
Beta strandi49 – 53Combined sources5
Turni56 – 58Combined sources3
Helixi60 – 66Combined sources7
Beta strandi79 – 83Combined sources5
Helixi89 – 98Combined sources10
Turni99 – 101Combined sources3
Beta strandi102 – 108Combined sources7
Helixi111 – 123Combined sources13
Beta strandi127 – 131Combined sources5
Helixi137 – 139Combined sources3
Turni140 – 143Combined sources4
Beta strandi147 – 152Combined sources6
Helixi159 – 170Combined sources12
Beta strandi171 – 181Combined sources11
Helixi183 – 186Combined sources4
Beta strandi188 – 190Combined sources3
Beta strandi192 – 195Combined sources4
Helixi198 – 205Combined sources8
Beta strandi208 – 215Combined sources8
Turni217 – 219Combined sources3
Beta strandi224 – 230Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ID5X-ray4.01B/F1-232[»]
3ID6X-ray2.60C1-232[»]
3PLAX-ray3.15E/F/M1-232[»]
5JPQelectron microscopy7.30W/X1-232[»]
ProteinModelPortaliP58032.
SMRiP58032.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP58032.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni89 – 90S-adenosyl-L-methionine binding2
Regioni108 – 109S-adenosyl-L-methionine binding2
Regioni133 – 134S-adenosyl-L-methionine binding2
Regioni153 – 156S-adenosyl-L-methionine binding4

Sequence similaritiesi

Belongs to the methyltransferase superfamily. Fibrillarin family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00078. Archaea.
COG1889. LUCA.
HOGENOMiHOG000106741.
InParanoidiP58032.
KOiK04795.
OMAiEYREWNL.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00351. RNA_methyltransf_FlpA. 1 hit.
InterProiIPR000692. Fibrillarin.
IPR020813. Fibrillarin_CS.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01269. Fibrillarin. 1 hit.
[Graphical view]
PIRSFiPIRSF006540. Nop17p. 1 hit.
PRINTSiPR00052. FIBRILLARIN.
SMARTiSM01206. Fibrillarin. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00566. FIBRILLARIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P58032-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEVITVKQT NMENIYECEF NDGSFRLCTR NLVPNFNVYG ERLIKYEGVE
60 70 80 90 100
YREWNAFRSK LAGAILKGLK TNPIRKGTKV LYLGAASGTT ISHVSDIIEL
110 120 130 140 150
NGKAYGVEFS PRVVRELLLV AQRRPNIFPL LADARFPQSY KSVVENVDVL
160 170 180 190 200
YVDIAQPDQT DIAIYNAKFF LKVNGDMLLV IKARSIDVTK DPKEIYKTEV
210 220 230
EKLENSNFET IQIINLDPYD KDHAIVLSKY KG
Length:232
Mass (Da):26,422
Last modified:April 27, 2001 - v1
Checksum:iCFFB34D2D4FA9CF2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK41216.1.
PIRiA99245.
RefSeqiWP_009992372.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK41216; AAK41216; SSO0940.
GeneIDi27427256.
KEGGisso:SSO0940.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK41216.1.
PIRiA99245.
RefSeqiWP_009992372.1. NC_002754.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ID5X-ray4.01B/F1-232[»]
3ID6X-ray2.60C1-232[»]
3PLAX-ray3.15E/F/M1-232[»]
5JPQelectron microscopy7.30W/X1-232[»]
ProteinModelPortaliP58032.
SMRiP58032.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48939N.
IntActiP58032. 2 interactors.
STRINGi273057.SSO0940.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK41216; AAK41216; SSO0940.
GeneIDi27427256.
KEGGisso:SSO0940.

Phylogenomic databases

eggNOGiarCOG00078. Archaea.
COG1889. LUCA.
HOGENOMiHOG000106741.
InParanoidiP58032.
KOiK04795.
OMAiEYREWNL.

Miscellaneous databases

EvolutionaryTraceiP58032.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00351. RNA_methyltransf_FlpA. 1 hit.
InterProiIPR000692. Fibrillarin.
IPR020813. Fibrillarin_CS.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01269. Fibrillarin. 1 hit.
[Graphical view]
PIRSFiPIRSF006540. Nop17p. 1 hit.
PRINTSiPR00052. FIBRILLARIN.
SMARTiSM01206. Fibrillarin. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00566. FIBRILLARIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFLPA_SULSO
AccessioniPrimary (citable) accession number: P58032
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: April 27, 2001
Last modified: November 2, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.